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Electron Microscopy Core Facility

The facility provides advanced expertise in electron microscopy, from sample preparation to image analysis, for a wide variety of biological samples.

Equipment

Electron Microscopes (EMCF)

These microscopes are maintained and managed by the EM core facility. We provide training and assistance to users who wish to use any of the equipment listed below.

MicroscopeFeaturesRoom
Morgagniscreening microscope, equipped with a side-mounted 1K CCD Camera (SIS)318
Biotwin CM120 Philipsmicroscope for screening and 2D acquisition, equipped with a bottom-mounted 1K CCD Camera (Keen View, SIS)305c
JEM 2100+, JEOLmicroscope for large-scale 2D screening and tomography. Flexible operation from 80-200kV.317
TECNAI F30, 300kV FEG, FEItomography microscope (plastic samples), and cryomicroscope, primarily for cellular tomography application322
CrossBeam 550, ZeissFocused Ion Beam-Scanning Electron Microscope (FIB-SEM) for large-volume 3D imaging.311
CrossBeam 540, ZeissFocused Ion Beam-Scanning Electron Microscope (FIB-SEM) for large-volume 3D imaging.319

Ultramicrotomes

For plastic sections

Conventional UltramicrotomesRoom
UC7, Leicaplastic-1325
UC7, Leicaplastic-2325
UC6, Leicaplastic/cryo327

For cryosections

Cryo-ultra-microtomesRoom
UC6, Leicacryo-3, for Tokuyasu cryosections325
UC6, Leicacryo-1, for frozen hydrated sections (and plastic)327

Sample preparation

Freeze Substitution Devices (FS) ─ room 305A

  • AFS-2a, Leica
    • process samples for manual freeze-substitution and plastic-embedding
  • AFS-2b, Leica
    • process samples for manual freeze-substitution and plastic-embedding
  • AFS-2c, Leica
    • process samples for manual freeze-substitution and plastic-embedding, acquired with the support of the HMLS

Accessory equipment

1 Knife-maker
used to prepare glass knives for ultramicrotomy (Leica) ─ room: 325

1 Freeze Substitution machine
Leica AFS1

Carbon coater
Leica EM ACE 600 acquired with the support of the HMLS ─ room: 312

Glow discharge device
room: 324

Electron Microscopes (SB)

The cryo-electron microscopes are maintained and managed by EMBL’s Structural and Computational Biology unit.

Service for single-particle and tomography cryo-EM for structural biology projects is offered through the cryo-EM service platform.

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