31 August - 5 September 2025
FISHing for RNAs: classical to single molecule approaches
Florence Besse, Imre Gaspar, Emerald Perlas, Elisabeth Zielonka
OverviewSpeakerProgrammePractical informationSponsorsMedia kit Overview Course overview Tight spatio temporal regulation of the number and localisation of RNA molecules is key to ensuring precise control of gene expression State of the art in situ hybridisation ISH technologies enable visualisation and quantification of RNA molecules in a biological context from organism and tissue to cell... OverviewSpeakerProgrammePractical informationSponsorsMedia kit Overview Course overview Tight spatio-temporal regulation of the number and localisation of RNA molecules is key to ensuring precise control of gene expression. State-of-the-art in situ hybridisation (ISH) technologies enable visualisation and quantification of RNA molecules in a biological context, from organism and tissue to cell. Moreover, with sequential ISH technologies (e.g. MER-FISH), the RNA signature of individual cells – or even their sub-compartments – can be identified and characterised in situ. This course – targeting early-stage scientists (PhD students and postdocs) – will highlight three powerful RNA visualisation techniques in histological and whole tissue samples. During this course, the participants will visualise subcellular localisation of selected transcripts through conventional ISH and more sensitive,...
EMBO Practical Course
EMBL Heidelberg
Closed