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Hands-on course for sample preparation and data analysis in proteomics – Course and Conference Office

EMBL Course

Hands-on course for sample preparation and data analysis in proteomics

Overview

Course overview

The course will give an introduction to the theoretical aspects of the analysis of proteins/peptides by mass spectrometry. Participants will get hands-on experience in sample preparation for a quantitative comparison across samples and perform a state of the art sample preparation and tandem mass tag labelling. Samples prepared by the participants will be measured by liquid chromatography-tandem mass spectrometry. In a session focused on bioinformatics analysis, participants will use FragPipe and FragPipe-Analyst in order to process the acquired data.

Audience

Applicants should have a basic understanding of protein biology and should know the principle of proteomics. The course is aimed at beginners who are new to proteomics or have minimal practical experience to start applying the acquired knowledge in their own research.

Modules/resources

  • Sample preparation
  • TMT theory and technical aspects
  • Phosphoproteomics
  • Introduction to MS technology
  • From spectra to spreadsheet tables with FragPipe
  • Data assessment (quality control) from spreadsheet outputs
  • Useful online tools for proteomics
  • Differential abundance analysis with FragPipe Analyst
  • Applications in proteomics

Learning outcomes

  • State of the art sample preparation
  • Learning which aspects of sample preparation can influence the outcome of the experiment
  • Getting insights into different proteomics applications
  • Understanding challenges working with PTMs
  • Fundamentals of proteomics data analysis
  • Visualisation of proteomics data

Participant commitment

Full-time attendance is required for the course duration.

What past participants say about the course

“The course was an incredible experience, combining well-structured theoretical sessions with exceptional hands-on wet lab practices. The unique opportunity to work directly in the lab was the highlight for me, making this course stand out among others. Highly recommended!” – Diego Márquez, University of Birmingham, UK

Speakers

Speakers, trainers, and staff

Isabelle Becher

EMBL Heidelberg

Germany

Dominic Helm

The University of Texas MD Anderson Cancer Center

USA

Jasmin Jansen

EMBL Heidelberg

Germany

Michal Nadler-Holly

Thermo Fisher Scientific

Germany

Mandy Rettel

EMBL Heidelberg

Germany

Nina Schmitt

EMBL Heidelberg

Germany

Jennifer Schwarz

EMBL Heidelberg

Germany

Frank Stein

EMBL Heidelberg

Scientific organisers

Dominic Helm

The University of Texas MD Anderson Cancer Center

USA

Mandy Rettel

EMBL Heidelberg

Germany

Frank Stein

EMBL Heidelberg

Germany

Course organisers

Lucía Rodriguez Minutta

EMBL Heidelberg

Germany

Michelle Steinhauer

EMBL Heildelberg

Germany

Programme

Are you on social media? Post using #EMBLProteomics and don’t forget to tag @EMBLEvents.

  • Some speakers may need to join virtually to give their lecture or Q&A, and the programme is subject to change
  • For registered participants, recorded talks will be accessible on demand for 4 weeks after the end of the event, unless indicated otherwise

Day 1: Tuesday, 16 June 2026

Time (Europe/Berlin)SpeakerLocation
08:30 – 09:00Bus from hotel to EMBL ATC EntrancePremier Inn Hotel
09:00 – 09:15Registration and welcomeFlex Lab
A & B
09:15 – 09:30Course overview and introduction to Proteomics Core FacilityFlex Lab
A & B
09:50 – 10:30Flash talks by participantsFlex Lab
A & B
10:30 – 10:45Coffee BreakCourtyard Room A & B
10:45 – 12:00Lecture: Sample preparation
Mandy Rettel – EMBL Heidelberg, Germany
Flex Lab
A & B
12:00 – 13:00LunchEMBL Canteen
13:00 – 13:15
Introduction to PracticalsFlex Lab A & B/ Training Lab B
13:15 – 15:15Practical 1: Cell lysis, Solubility assay, Benzonase treatment, Protein determination by Tryptophan assayTraining Lab B
15:15 – 15:45Coffee BreakCourtyard Room A & B
15:45 – 17:45Practical 2: Reduction & alkylation, SP3 protocol & digestion, Chloroform/Methanol precipitation & digestionTraining Lab B
17:45 – 18:30MS- GameFlex Lab A & B/ Training Lab B
18:30 – 20:30DinnerEMBL Canteen

Day 2: Wednesday, 17 June 2026

Time (Europe/Berlin)SpeakerLocation
09:00 – 09:10Overview of the dayFlex Lab A & B/ Training Lab B
09:10 – 10:25Practical 3: SDC precipitation and desalting Phospho sample, Peptide extraction & TMT labelling of solubility sampleTraining Lab B
10:25 – 10:55Lecture: TMT theory and technical aspects
Michal Nadler-Holly – Thermo Fisher Scientific, Germany
Flex Lab A & B
10:55 – 11:10Coffee break & Quenching at end of coffee break
Courtyard Room A & B
11:10 – 12:10Lecture: Phosphoproteomics
Jennifer Schwarz – EMBL Heidelberg, Germany
Flex Lab A & B
12:10 – 13:10LunchEMBL Canteen
13:10 – 14:10Practical 4: Multiplexing, Sample desalting, Fe-IMAC enrichmentFlex Lab A & B
14:15 – 15:40Lecture: Introduction to MS technology
Dominic Helm – MD Anderson Cancer Center, USA
Flex Lab A & B
15:40 – 16:10Q&A session with coffee
16:10 – 16:55Practical 5: Load samples on Mass spec & Facility tourProteomics Core Facility
16:55 – 17:30Lecture: Workflow overview
Frank Stein – EMBL Heidelberg, Germany
Flex Lab A & B
17:30 –Free evening
Day 3: Thursday, 18 June 2026

Time (Europe/Berlin)SpeakerLocation
09:00 – 09:10Overview of the dayComputer Training Lab
09:10 – 09:40Lecture: Raw file analysis with Freestyle
Michal Nadler Holly – Themo fisher scientific, Germany
Computer Training Lab
09:40 – 10:30Lecture: Raw file processing with FragPipe
Frank Stein – EMBL Heidelberg, Germany
Computer Training Lab
10:30 – 10:50Coffee breakCourtyard Room A & B
10:50 – 12:00Computer Practical: Analysis of own solubility data with FragPipeComputer Training Lab
12:00 – 13:00LunchEMBL Canteen
13:00 – 14:00Lecture: What data can tell you about your experimental workflow?
Frank Stein – EMBL Heidelberg, Germany
Computer Training Lab
14:00 – 14:25Coffee breakCourtyard Room A & B
14:25 – 16:25Practical: Analysis of own Phosphoproteomics data with FragPipeComputer Training Lab
16:25 – 16:50Coffee breakCourtyard Room A & B
16:50 – 17:50Computer Practical: Usage of bioinformatics tools
Jennifer Schwarz –
EMBL Heidelberg, Germany
Computer Training Lab
17:50 – 18:05Bus to restaurant
18:05 – 20:05Outside Dinner
20:05 – 20:20Bus return to hotel
Day 4: Friday, 19 June 2026

Time (Europe/Berlin)SpeakerLocation
09:00 – 09:10Overview of the dayFlex Lab A & B
09:10 – 10:25Lecture: Quantitative and statistical data analysis workflow
Frank Stein – EMBL Heidelberg, Germany
Computer Training Lab
10:25 – 10:45Coffee breakCourtyard Room A & B
10:45 – 11:45Computer Practical: Fragpipe Analyst solubility dataComputer Training Lab
11:45 – 12:45LunchEMBL Canteen
12:45 – 13:45Computer Practical: Fragpipe Analyst phospho dataComputer Training Lab
13:45 – 14:30Lecture: Applications of proteomics
Mikhail Savitski – EMBL Heidelberg, Germany
Flex Lab A & B
14:30 – 16:30Q&A session: Discussion of individual projects with PCFFlex Lab A & B
16:30 – 16:45Final remarks and closingFlex Lab A & B
16:45Bus to Heidelberg train station and city center

Practical information

Registration fees

The course is limited to 16 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.

Registration fees

Your registration for this course includes:

  • Admission to the event
  • Possibility to submit an abstract (if applicable)
  • Meals and coffee breaks
  • Course materials
  • Access to the e-learning platform
  • A letter to support your visa application (for more information, check the ‘Travel and accommodation’ tab)

Accommodation and travel are not included in the fees — participants are expected to book and pay their own expenses.

Academia€615
PhD Student€615
Industry€1125

Confirmation and payment

The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.

Cancellation policy

If you are no longer able to take part in the course, please inform your course contact responsible for this event. Your registration and the submitted motivation letter will be deleted.

Please also check our Terms and Conditions for the cancellation policy.

Further details

For further information about registration, please refer to the FAQ page.

Submitting a motivation letter

After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.

Instructions

  1. Include your relevant skills, experience and qualifications showing why you would be suitable for the course.
  2. Explain why you would like to attend, including what you can contribute and how you think you will benefit from the course.

Please note:

  • The limit of 2,000 characters refers to manually typed text and excludes spaces. If an error occurs try using a different web browser (preferably Google Chrome or Mozilla Firefox).
  • If you copy-paste the text into the form, hidden formatting might still be included which may cause the text to exceed the 2,000 character limit resulting in an error message. We recommend you clear all formatting before pasting in the text.
  • If you have special symbols in your text, make sure you are using Unicode characters, otherwise these will not be recognised.

For detailed instructions, please watch our video on how to submit a course motivation letter.

Further details

For further information about registration and motivation letter submission please refer to the FAQ page.

Financial assistance

Financial assistance

Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of registration fee waiverstravel grants and childcare grants

Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.

Registration fee waiver

The fee waiver will cover the registration sum that you have paid to attend the course.

Travel grant

The travel grant will cover the cost of travel (airfare, train, bus, taxi, accommodation, visa) and is provided up to specified caps which are normally as follows:

– up to €400 for participants travelling to an EMBL Course within Europe.

– up to €1000 for participants travelling to an EMBL Course from outside Europe.

The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.

Childcare grants

There is the possibility to apply for a childcare grant to offset child care costs incurred by participants, speakers, trainers and organisers when attending a course. Eligible costs include (but are not limited to) fees for a babysitter or child-care facility and travel costs for a caregiver. Please note that priority will be given to early-stage researchers. There is a limited amount of funding available for the childcare grants and funds will be distributed amongst eligible applicants.

Boehringer Ingelheim Fonds travel grant

Applies to selected courses only. Availability will be indicated during the abstract or motivation letter submission process.

This grant covers costs related to your attendance to the course (registration, travel and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.
Whether you are eligible to apply for a travel grant, depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):

– for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course
– for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course

Application

You can apply for financial assistance in the submission portal by the abstract deadline. Read the instructions on how to apply for financial assistance. Only submissions for financial assistance will be accepted. Presentation abstracts cannot be submitted here and will be declined. 

In your application you will be asked to summarise your current work,  answer questions regarding why your lab cannot fund your attendance, and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.

*For some events, applications for Childcare Grants will still be done by email. Information about the grant will be sent out shortly after the abstract/motivation letter deadline. Please contact the event Conference Officer if you have any questions.

Selection

A selection process will take place with the results announced by email to all applicants / submitters. We aim to announce 3 weeks (21 days) after the abstract submission deadline but delays may occur.

Selection results do not impact your admission to the meeting. Selection for registration fee waivers and travel grants is based on scientific merit, your current work or study location, the reasons for needing financial support, and the impact this event will have on your career.

Childcare grants will be allocated in the same timeframe (6-8 weeks before the event start date). Please note that priority will be given to early-stage researchers.

Further details

Check out this list of external funding opportunities

For further information about financial assistance please refer to the FAQ page.

Travel and accommodation

Visa

We can send you a registration confirmation to support your visa procedure (not an official invitation letter). Please contact the Conference Officer responsible for your event. Read our FAQ for more information.

Travel

If you are travelling to Heidelberg via aeroplane, train, car, or long-distance bus, you can find useful information on our travel information page.

Accommodation

Accommodation is not included in the registration fee.

A mix of single and twin rooms have been reserved for 3 nights from 16 to 19 June 2026 at the Premier Inn hotel. Single rooms are limited and will be available on a first come, first served basis.

After the selection process, selected participants should contact the hotel directly to make a booking with a booking code (code of your course, e.g. SDP26-01) by the deadline of 19 May 2026.

We recommend that all course participants stay at the dedicated hotel for the course.

Local transportation

Course shuttle buses

Shuttle buses will go from the Premier Inn Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.

Other local transportation

In addition to the shuttle busses, there is also the public bus 28 that serves the EMBL campus and taxis can be easily booked at any time. For more information,  please refer to the local transportation (RNV) website.

You may also walk from the ISG Hotel to EMBL (takes up to 30 minutes)

Map to walk from ISG Hotel to EMBL

Further details

Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

For more information about travel and accommodation, please refer to the FAQ page.

On-site information

Here you will find useful information for when you are onsite at the event.

Catering

All meals and coffee breaks are included in the registration fee. Our catering staff will prepare a variety of vegetarian meals, meat and fish dishes, soups, pasta, fresh fruit and vegetables, as well as a variety of desserts.

Please wear your badge at all times when serving yourself.

No food or drinks are allowed in the auditorium.

Internet

Wi-Fi is available on campus using the EMBL-Events network and the event specific password, which will be provided on site. The eduroam network (secure, worldwide roaming access service developed for the international research and education community) is also available.

Photography

During the conference, an EMBL Photographer may be taking photos. If you would not like to appear in these, please inform the photographer or a member of the Course and Conference Office.

Charging stations

There are lockers available next to the stairs leading down into the Auditorium. You will find some of those equipped with sockets to charge your smartphone/tablet etc.

In most places the electricity is 220 volts AC (50 cycles). An adaptor and a plug that fits the German socket may be needed for your appliances/laptop (i.e. American, Japanese, etc.). A USB charging station for electronic devices is available at the registration desk.

Printing

We can help to print your boarding pass/train ticket. Please send it to events@embl.de and collect your print-outs at the registration desk.

Luggage

There are lockers available on-site to store your luggage, which require a 2 EURO coin to operate. There is another luggage room on level E0, which is free to use but remains unlocked during the conference.

Lost and found

‘’Lost and Found’’ items are kept at the registration desk until the end of the conference.

EMBL merchandise

If you are interested in purchasing EMBL merchandise (products presented in the glass display in the registration area), please email the EMBL shop to place an order.

Kindly note the EMBL shop is only open upon request and all purchases must be made in cash (Euros only).

Nursing room

There is a nursing room available in the ATC Rooftop Lounge on level A29.

Room for prayers, meditation, and yoga

There is a room for prayer, yoga, and meditation, located on level E0 behind the Auditorium. Please be respectful of others using the room.

EMBL campus map

Health and safety notes

Please take a moment to read our health and safety policy.

  • Do not smoke in any EMBL building.
  • Eating and drinking is prohibited in the Auditorium and all laboratories.
  • Do not enter any restricted areas or the laboratories unless instructed to do so.

If first aid is required

  • The first aid room is located next to the ATC Registration Desk (room 10- 205).
  • Dial the Emergency number 222 from any EMBL internal phone only.
  • Be prepared to answer the following questions: Where is the accident? What happened? How many injured?
  • Emergency number 06221-387 7821 from mobile phones.
  • Please report all accidents to the conference officer at the registration desk.

In case of fire

  • Press the nearest fire alarm button or the Emergency number 222 from any internal phone.
  • A loud fire alarm will go off if an evacuation is required. On hearing the alarm leave the building immediately following the escape route and fire wardens and head to one of the meeting points
  • Do not use the lifts.

Beyond first aid

The Heidelberg University Hospital or ‘Universitätsklinikum‘ has a 24 hour accident and emergency facility and is at ‘Im Neuenheimer Feld 672’. It is a 8km or a 20 minute taxi ride away from EMBL. In a medical emergency, the ambulance service generally transports patients to this hospital.

Please remember to bring your own medication, if needed, to the conference. Note that the next pharmacy is a 4-minute drive from the EMBL, but for many medications you will be required to see a doctor to get a prescription.

Ensure in advance that your medical insurance will cover you during your visit in the event that you do need to see a doctor while in Heidelberg.

The EMBL course and conference office team are able to advise you on where to turn for emergency or non-emergency medical treatment in Heidelberg and can assist you in making appointments and / or arranging transport to get to the pharmacy or a doctor of your choice if necessary. Please note, we are not however permitted to personally accompany you off campus.

Sightseeing

A variety of activities in Heidelberg can be found on the website of Heidelberg Marketing.

Technical requirements and event software

(delete if no technical requirements for this course!)

Technical requirements

Below you will find the software and technical requirements for this course. You will need admin rights to be able to install the software on your computer and we recommend you try this before the course. If you do not have admin rights, please contact your technical support team in advance to make sure the software can be installed.

Add your software name here

Add a descritpion about your software here and any other important info

Additional software

The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus. 

Zoom will be used for the live talks and Q&A sessions. More information will be provided closer to the start of the course.

Other information

While using the software during the course, please make sure you have nothing else using your bandwidth. We recommend using a wired connection.

Virtual participation guidelines

DELETE THIS TAB IF COURSE IS NOT HYBRID!!

Guidelines

  • Do not broadcast the course to unregistered participants.
  • Do not share your login details with others.
  • You are encouraged to post on social media about the event. Post unless the speaker specifically says otherwise, but be mindful of unpublished data. 
  • Please do not capture, transmit or redistribute data presented at the meeting.
  • Video library of the recorded talks during the event and for 4 weeks afterwards. Please note: at the request of individual speakers, some talks are not recorded.

Additional information can be found in our Code of Conduct.

How to ask questions

Questions during and after the talks can be asked in the live streaming platform. If time runs out or you think of a question later, you can use the EMBL eCampus forum or send a direct message to the speaker/trainer.

Time zone

The programme is planned based on the Europe/Berlin time zone, unless otherwise stated. As many virtual participants are attending from around the world, we do our best to accommodate as many time zones as possible when creating the programme. Please take your time zone into consideration when planning your attendance.

Additional information

Please find additional information including FAQs and terms and conditions on our Information for Participants page.

Sponsors

Sponsorship opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other conferences, or contact sponsorship@embl.de for further information.

If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.

Warning

EMBL advises sponsors to beware of fraudulent schemes falsely offering sponsorship opportunities. All legitimate communication about sponsorship for EMBL conferences, symposia, and courses comes directly from EMBL via official EMBL email accounts. EMBL does not use third parties for sponsorship acquisition.

– EMBL does not sell attendee lists.
– EMBL will never ask for credit card details or direct payments.
– All payments are processed by invoice only.

If you receive a suspicious message claiming to be from or on behalf of EMBL, please report it to sponsorship@embl.org.

Media Kit

Want to let others know you’re attending this event? Take a look at our shareable media and feel free to use them in your social media channels or presentations.

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Date: 16 - 19 Jun 2026

Location: EMBL Heidelberg

Venue: EMBL Advanced Training Centre


Deadline(s):

Application: 3 Mar 2026


Organisers:


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