17 May - 22 May 2026
Advanced fluorescence imaging techniques
Aliaksandr Halavatyi, Joana Delgado Martins, Manuel Gunkel, Robert Kasper
Description OverviewSpeakersProgrammePractical informationSponsorsMedia kit Overview Course overview This practical course will cover advanced light microscopy techniques and participants will learn how to derive qualitative and quantitative insights into molecular mechanisms in cells tissues and developing organisms Guest researchers together with microscopy experts from EMBL will foster an intensive exchange of information through a balance of lectures and practical workshops The focus of the course will be on the use of fluorescence microscopy to obtain information about the localisation and functions of proteins and other biomolecules at different spatial scales Audience This course is directed towards researchers in the life sciences with access to light microscopy equipment who have an immediate need to apply the techniques they will learn to solve biological problems at the cellular and molecular level in vivo As the course will primarily focus on advanced techniques we expect participants to have basic knowledge of light microscopy Modules resources The course will include lectures and hands on practicals covering multiple fluorescence microscopy techniques Fast time lapse microscopy of living cells Spinning disk and point scanning confocal microscopy of large 3D samples Two photon microscopy and Second Harmonic Generation SHG imaging Photo manipulation as a tool Multicolour single molecule localisation microscopy SMLM with spectral demixing and DNA PAINT Expansion microscopy FRET based techniques Microscope automation For demanding applications we will cover the aspects of image processing and analysis The modules will cover all the necessary knowledge to conduct similar pipelines in your home lab enabling you to answer emerging biological questions Learning outcomes Get an overview of advanced fluorescence imaging techniques Obtain hands on experience setting up and running advanced experiments on modern fluorescence microscopes Discuss the possibilities and drawbacks of experiment planning and data interpretation with experts in the field This course is kindly supported by the core facility EMBL Advanced Light Microscopy Facility Participant commitment The course includes recorded live lectures interactive discussion sessions practicals hands on exercises student presentations and networking Full time attendance is required throughout the course Frequently Asked Questions Will image analysis topics be covered during the course Dedicated image data processing practicals for improving image quality through deconvolution and for post processing data acquired during the practicals will be included in the course Aspects of image analysis will also be part of the microscope automation practical Because of time limitations our course can not include comprehensive image analysis training covering all basic and different advanced image analysis topics Before the course we will provide self training material covering the image analysis knowledge required to follow the course If you are specifically interested in gaining in depth knowledge of image analysis we suggest paying attention to upcoming image analysis courses Can participants bring their image samples to the course The course schedule is very tight Therefore we unfortunately cannot offer sessions where course participants can experiment with their own samples The samples for each practical will be provided by the course organisers For participants who do not have access to the necessary techniques at their home institutions we will include a discussion session on how to access light microscopy facilities or other research infrastructures at EMBL or other institutions to perform high end experiments Which microscopes will be used for hands on practicals Evident together with its partner companies will set up four high end microscopes needed to perform all techniques learned during the course What past participants say about the course I highly recommend this course if you are looking to take your fluorescence microscopy experiments to the next level Josefina Marin Rojas Universidad de Chile Chile Speakers Speakers trainers and staff Niccolo Banterle EMBL Heidelberg Germany Laura Batti Wyss Center for Bio and Neuro Engineering Switzerland S bastien Bellow Abbelight France Steven Coleman VisiTech International UK Joana Delgado Martins University of Zurich Switzerland Ulrike Endesfelder University of Bonn Germany Martin Fritsch EMBL Heidelberg Germany Manuel Gunkel EMBL Heidelberg Germany Aliaksandr Halavatyi EMBL Heidelberg Germany Mark Hink University of Amsterdam The Netherlands Marie Jacobovitz EMBL Heidelberg Germany Robert Kasper Evident Germany Muzamil Khan EMBL Heidelberg Germany Steffen Klein EMBL Heidelberg Germany Simone K hler EMBL Heidelberg Germany Guy Malkinson Universit de Lorraine France Priyesh Parihar EMBL Heidelberg Germany Marianna Poli Euro BioImaging Germany Sinem Saka EMBL Heidelberg Germany Caroline Simon EMBL Heidelberg Germany Mereke Suleimenov EMBL Heidelberg Germany Stefan Terjung EMBL Heidelberg Germany Vishwachi Tripathi EMBL Heidelberg Germany Urs Ziegler University of Zurich Switzerland Anja Zimmermann Rapp OptoElectronic Germany Scientific organisers Joana Delgado Martins University of Zurich Switzerland Manuel Gunkel EMBL Heidelberg Germany Aliaksandr Halavatyi EMBL Heidelberg Germany Robert Kasper Evident Germany Course organisers Luana Ribeiro Course and Conference Officer EMBL Heidelberg Germany Andrea Coti Scientific Training Officer EMBL Heidelberg Germany Ellen Kierstein Scientific Training Officer EMBL Heidelberg Germany Programme Are you on social media Post using EMBLFluorImaging and don t forget to tag EMBLEvents Some pre course work such as watching pre recordings and protocol reading with estimated time 0 10 hours will be required and will be shared in advance of the course Some speakers may need to join virtually to give their lecture or Q A and the programme is subject to change For registered participants recorded talks will be accessible on demand for 4 weeks after the end of the event unless indicated otherwise Day 1 Sunday 17 May 2026 Time Europe Berlin SpeakerLocation15 30 16 00Welcome and house notes and course overview and FAIR data guidelinesISG Hotel16 00 17 00Lecture Concepts in light microscopyUrs Ziegler University of Zurich SwitzerlandISG Hotel17 00 17 30Coffee breakISG Hotel17 30 18 15Lecture Live cell imagingJoana Delgado Martins University of Zurich SwitzerlandISG Hotel18 15 19 15Lecture Adaptive feedback microscopyAliaksandr Halavatyi EMBL Heidelberg GermanyISG Hotel19 15 21 15DinnerISG Hotel Day 2 Monday 18 May 2026 Time Europe Berlin SpeakerLocation09 00 09 05Overview of the dayCourtyard Room A B09 05 09 50Lecture An introduction to image scanning microscopeSteven Coleman VisiTech International UKCourtyard Room A B09 50 10 45Lecture Expansion microscopyNiccolo Banterle EMBL Heidelberg GermanyCourtyard Room A B10 45 11 00Coffee break11 00 12 30Practical Preparation of expansion microscopy samplesCaroline Simon EMBL Heidelberg GermanyTraining lab12 30 13 30LunchEMBL Canteen13 30 13 40Introduction to the practicalsCourtyard Room A B13 40 16 10Practical Session 1 Live cell imagingJoana Delgado University of Zurich SwitzerlandSteven Coleman VisiTech International UK Group 1 3D imaging of model organisms with CLSMMartin Fritsch EMBL Heidelberg GermanyManuel Gunkel EMBL Heidelberg Germany Groups 3 4 Imaging expanded samplesCaroline Simon EMBL Heidelberg Germany Group 2 Microscopy rooms16 10 16 30Coffee break16 30 19 00Practical Session 2 Live cell imagingJoana Delgado University of Zurich SwitzerlandSteven Coleman VisiTech International UK Group 4 3D imaging of model organisms with CLSMMartin Fritsch EMBL Heidelberg GermanyManuel Gunkel EMBL Heidelberg Germany Groups 1 2 Imaging expanded samplesCaroline Simon EMBL Heidelberg Germany Group 3 Microscopy rooms19 00 21 00Pizza dinner and flash talksRooftop Lounge Day 3 Tuesday 19 May 2026 Time Europe Berlin SpeakerLocation09 00 09 05Overview of the dayCourtyard Room A B09 05 10 05Lecture Whole organ lightsheet microscopy as a tool for neuroscienceLaura Batti Wyss Center for Bio and Neuro Engineering SwitzerlandCourtyard Room A B10 05 11 05Lecture Introduction to SMLMS bastien Bellow Abbelight FranceCourtyard Room A B11 05 11 30Coffee break11 30 12 30Lecture Single molecule microscopy and tracking as a quantitative tool for biochemical and cell biological in vivo studies in micro biologyUlrike Endesfelder University of BonnCourtyard Room A B12 30 13 30LunchEMBL canteen13 30 13 40Introduction to the practicalsCourtyard Room A B13 40 16 10Practical Session 3 Live cell imagingJoana Delgado University of Zurich SwitzerlandSteven Coleman VisiTech International UK Group 2 DeconvolutionUrs Ziegler University of Zurich SwitzerlandMartin Fritsch EMBL Heidelberg GermanyVishwachi Tripathi EMBL Heidelberg Germany Groups 3 4 Imaging expanded samplesCaroline Simon EMBL Heidelberg Germany Group 1 Microscopy rooms Computer Training Lab16 10 16 30Coffee break16 30 19 00Practical Session 4 Live cell imagingJoana Delgado University of Zurich SwitzerlandSteven Coleman VisiTech International UK Group 3 DeconvolutionUrs Ziegler University of Zurich SwitzerlandMartin Fritsch EMBL Heidelberg GermanyVishwachi Tripathi EMBL Heidelberg Germany Groups 1 2 Imaging expanded samplesCaroline Simon EMBL Heidelberg Germany Group 4 Microscopy rooms Computer Training Lab19 00 21 00Tapas dinner and flash talks Day 4 Wednesday 20 May 2026 Time Europe Berlin SpeakerLocation09 00 09 05Overview of the dayCourtyard Room A B09 05 10 05Lecture Studying biological tissues using contrast mechanisms generated by two photon excitation microscopyGuy Malkinson Universit de Lorraine FranceCourtyard Room A B10 05 10 45Lecture Photomanipulation techniques and applicationsAnja Zimmermann Rapp OptoElectronic GmbHCourtyard Room A B10 45 11 05Coffee break11 05 11 30Lecture How Euro BioImaging can support your research with access to the best imagingMarianna Childress Poli EuroBioImagingCourtyard Room A B11 30 12 30Lecture Measuring interactions and biosensors using FRETMark Hink University of Amsterdam The NetherlandsCourtyard Room A B12 30 13 30LunchEMBL canteen13 30 13 40Introduction to the practicalsCourtyard Room A B13 40 16 10Practical Session 5 Multicolor single molecule localization microscopy with spectral demixingS bastien Bellow Abbelight FranceManuel Gunkel EMBL Heidelberg Germany Group 2 Multiphoton imaging and Second Harmonic Generation SHG Robert Kasper Evident GermanyGuy Malkinson Universit de Lorraine France Group 1 FRETMark Hink University of Amsterdam The NetherlandsStefan Terjung EMBL Heidelberg Germany Group 4 Applying adaptive feedback microscopy for high throughput photoactivation of specific cell phenotypesAliaksandr Halavatyi EMBL Heidelberg GermanyAnja Zimmermann Rapp OptoElectronic GmbH Germany Group 3 Microscopy rooms16 10 16 30Coffee break16 30 19 00Practical Session 6 Multicolor single molecule localization microscopy with spectral demixingS bastien Bellow Abbelight FranceManuel Gunkel EMBL Heidelberg Germany Group 4 Multiphoton imaging and Second Harmonic Generation SHG Robert Kasper Evident GermanyGuy Malkinson Universit de Lorraine France Group 3 FRETMark Hink University of Amsterdam The NetherlandsStefan Terjung EMBL Heidelberg Germany Group 2 Applying adaptive feedback microscopy for high throughput photoactivation of specific cell phenotypesAliaksandr Halavatyi EMBL Heidelberg GermanyAnja Zimmermann Rapp OptoElectronic GmbH Germany Group 1 Microscopy rooms19 00 22 00Dinner downtown Day 5 Thursday 21 May 2026 Time Europe Berlin SpeakerLocation09 00 09 05Overview of the dayCourtyard Room A B09 05 10 05Lecture Visualizing chromosomes during meiosisSimone K hler EMBL Heidelberg GermanyCourtyard Room A B10 05 11 05Lecture Development of novel protein tags NovoTags for multiplex fluorescence imaging and cryogenic correlative light and electron microscopy cryo CLEM Steffen Klein EMBL Heidelberg GermanyCourtyard Room A B11 05 11 30Coffee break11 30 12 30Lecture A DNA toolbox for spatial biologySinem Saka EMBL Heidelberg GermanyCourtyard Room A B12 30 13 30LunchEMBL Canteen13 30 13 40Introduction to the practicalsCourtyard Room A B13 40 16 10Practical Session 7 Multicolor single molecule localization microscopy with spectral demixingS bastien Bellow Abbelight FranceManuel Gunkel EMBL Heidelberg Germany Group 1 Multiphoton imaging and Second Harmonic Generation SHG Robert Kasper Evident GermanyGuy Malkinson Universit de Lorraine France Group 2 FRETMark Hink University of Amsterdam The NetherlandsStefan Terjung EMBL Heidelberg Germany Group 3 Applying adaptive feedback microscopy for high throughput photoactivation of specific cell phenotypesAliaksandr Halavatyi EMBL Heidelberg GermanyAnja Zimmermann Rapp OptoElectronic GmbH Germany Group 4 Microscopy rooms16 10 16 30Coffee break16 30 19 00Hands on Practical Session 8 Multicolor single molecule localization microscopy with spectral demixingS bastien Bellow Abbelight FranceManuel Gunkel EMBL Heidelberg Germany Group 3 Multiphoton imaging and Second Harmonic Generation SHG Robert Kasper Evident GermanyGuy Malkinson Universit de Lorraine France Group 4 FRETMark Hink University of Amsterdam The NetherlandsStefan Terjung EMBL Heidelberg Germany Group 1 Applying adaptive feedback microscopy for high throughput photoactivation of specific cell phenotypesAliaksandr Halavatyi EMBL Heidelberg GermanyAnja Zimmermann Rapp OptoElectronic GmbH Germany Group 2 Microscopy rooms19 00 20 30DinnerEMBL Canteen Day 6 Friday 22 May 2026 Time Europe Berlin SpeakerLocation09 00 09 05Overview of the dayComputer training lab09 05 10 35Practical FRET data analysisMark Hink University of Amsterdam The NetherlandComputer training lab10 35 11 00Coffee break11 00 12 30Practical SMLM data analysisS bastien Bellow Abbelight FranceManuel Gunkel EMBL Heidelberg GermanyComputer training lab12 30 13 30LunchEMBL Canteen13 30 15 00Preparation of student presentationsComputer training lab15 00 16 30Student presentationsComputer training lab16 30 17 00Wrap up of course and departureComputer training lab Practical information Registration fees The course is limited to 16 participants For selection purposes please note that your application will not be considered without a letter of motivation Registration fees Your registration for this course includes Admission to the event Possibility to submit an abstract if applicable Meals and coffee breaks Course materials Access to the e learning platform A letter to support your visa application for more information check the Travel and accommodation tab Accommodation and travel are not included in the fees participants are expected to book and pay their own expenses Academia 650PhD Student 650Industry 1150 Confirmation and payment The registration fee should be paid only after acceptance to the course The results will be announced approximately 2 3 weeks after the application deadline Cancellation policy If you are no longer able to take part in the course please inform your course contact responsible for this event Your registration and the submitted motivation letter will be deleted Please also check our Terms and Conditions for the cancellation policy Further details For further information about registration please refer to the FAQ page Submitting a motivation letter After you have logged in and successfully registered you will receive an email asking you to submit your motivation letter Click on the link provided and enter your motivation letter in the text box provided Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering Instructions Include your relevant skills experience and qualifications showing why you would be suitable for the course Explain why you would like to attend including what you can contribute and how you think you will benefit from the course Please note The limit of 2 000 characters refers to manually typed text and excludes spaces If an error occurs try using a different web browser preferably Google Chrome or Mozilla Firefox If you copy paste the text into the form hidden formatting might still be included which may cause the text to exceed the 2 000 character limit resulting in an error message We recommend you clear all formatting before pasting in the text If you have special symbols in your text make sure you are using Unicode characters otherwise these will not be recognised For detailed instructions please watch our video on how to submit a course motivation letter Further details For further information about registration and motivation letter submission please refer to the FAQ page Financial assistance Financial assistance Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of registration fee waivers travel grants and childcare grants Your place in the meeting is only confirmed by paying the registration fee which is mandatory even when receiving a fee waiver Registration fee waiver The fee waiver will cover the registration sum that you have paid to attend the course Travel grant The travel grant will cover the cost of travel airfare train bus taxi accommodation visa and is provided up to specified caps which are normally as follows up to 400 for participants travelling to an EMBL Course within Europe up to 1000 for participants travelling to an EMBL Course from outside Europe The organisers may reduce the grant cap to accommodate more participants Recipients will be notified of their travel cap amount when they are informed of the outcome of their application Original receipts must be provided with your signature for all costs incurred within two months of completion of travel Scanned copies cannot be accepted Childcare grants There is the possibility to apply for a childcare grant to offset child care costs incurred by participants speakers trainers and organisers when attending a course Eligible costs include but are not limited to fees for a babysitter or child care facility and travel costs for a caregiver Please note that priority will be given to early stage researchers There is a limited amount of funding available for the childcare grants and funds will be distributed amongst eligible applicants Boehringer Ingelheim Fonds travel grant Applies to selected courses only Availability will be indicated during the abstract or motivation letter submission process This grant covers costs related to your attendance to the course registration travel and accommodation costs The grant is restricted to PhD students and postdocs who conduct basic biomedical research Whether you are eligible to apply for a travel grant depends on when you received your university entrance qualification e g Abitur A Levels High School Diploma Final State Examination for PhD and MD students as well as graduates the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course for postdocs the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course Application You can apply for financial assistance in the submission portal by the abstract deadline Read the instructions on how to apply for financial assistance Only submissions for financial assistance will be accepted Presentation abstracts cannot be submitted here and will be declined In your application you will be asked to summarise your current work answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career Application for financial support will not affect the outcome of your registration application For some events applications for Childcare Grants will still be done by email Information about the grant will be sent out shortly after the abstract motivation letter deadline Please contact the event Conference Officer if you have any questions Selection A selection process will take place with the results announced by email to all applicants submitters We aim to announce 3 weeks 21 days after the abstract submission deadline but delays may occur Selection results do not impact your admission to the meeting Selection for registration fee waivers and travel grants is based on scientific merit your current work or study location the reasons for needing financial support and the impact this event will have on your career Childcare grants will be allocated in the same timeframe 6 8 weeks before the event start date Please note that priority will be given to early stage researchers Further details Check out this list of external funding opportunities For further information about financial assistance please refer to the FAQ page Travel and accommodation Visa We can send you a registration confirmation to support your visa procedure not an official invitation letter Please contact the Conference Officer responsible for your event Read our FAQ for more information Travel If you are travelling to Heidelberg via aeroplane train car or long distance bus you can find useful information on our travel information page Accommodation Accommodation is not included in the registration fee A mix of single and twin rooms have been reserved for 6 nights from 16 May to 22 May at the ISG hotel Single rooms are limited and will be available on a first come first served basis After the selection process selected participants should contact the hotel directly to make a booking with a booking code EVA26 01 by the deadline of 17 April 2026 We recommend that all course participants stay at the dedicated hotel for the course Local transportation Course shuttle buses Shuttle buses will go from the ISG Hotel to EMBL and back mornings and evenings A bus schedule and location of the bus stops will be made available prior to the meeting Other local transportation In addition to the shuttle busses there is also the public bus 28 that serves the EMBL campus and taxis can be easily booked at any time For more information please refer to the local transportation RNV website You may also walk from the ISG Hotel to EMBL takes up to 30 minutes Map to walk from ISG Hotel to EMBL Further details Address EMBL Heidelberg Meyerhofstra e 1 69117 Heidelberg Germany For more information about travel and accommodation please refer to the FAQ page On site information Here you will find useful information for when you are onsite at the event Catering All meals and coffee breaks are included in the registration fee Our catering staff will prepare a variety of vegetarian meals meat and fish dishes soups pasta fresh fruit and vegetables as well as a variety of desserts Please wear your badge at all times when serving yourself No food or drinks are allowed in the auditorium Internet Wi Fi is available on campus using the EMBL Events network and the event specific password which will be provided on site The eduroam network secure worldwide roaming access service developed for the international research and education community is also available Photography During the conference an EMBL Photographer may be taking photos If you would not like to appear in these please inform the photographer or a member of the Course and Conference Office Charging stations There are lockers available next to the stairs leading down into the Auditorium You will find some of those equipped with sockets to charge your smartphone tablet etc In most places the electricity is 220 volts AC 50 cycles An adaptor and a plug that fits the German socket may be needed for your appliances laptop i e American Japanese etc A USB charging station for electronic devices is available at the registration desk Printing We can help to print your boarding pass train ticket Please send it to events embl de and collect your print outs at the registration desk Luggage There are lockers available on site to store your luggage which require a 2 EURO coin to operate There is another luggage room on level E0 which is free to use but remains unlocked during the conference Lost and found Lost and Found items are kept at the registration desk until the end of the conference EMBL merchandise If you are interested in purchasing EMBL merchandise products presented in the glass display in the registration area please email the EMBL shop to place an order Kindly note the EMBL shop is only open upon request and all purchases must be made in cash Euros only Nursing room There is a nursing room available in the ATC Rooftop Lounge on level A29 Room for prayers meditation and yoga There is a room for prayer yoga and meditation located on level E0 behind the Auditorium Please be respectful of others using the room EMBL campus map Health and safety notes Please take a moment to read our health and safety policy Do not smoke in any EMBL building Eating and drinking is prohibited in the Auditorium and all laboratories Do not enter any restricted areas or the laboratories unless instructed to do so If first aid is required The first aid room is located next to the ATC Registration Desk room 10 205 Dial the Emergency number 222 from any EMBL internal phone only Be prepared to answer the following questions Where is the accident What happened How many injured Emergency number 06221 387 7821 from mobile phones Please report all accidents to the conference officer at the registration desk In case of fire Press the nearest fire alarm button or the Emergency number 222 from any internal phone A loud fire alarm will go off if an evacuation is required On hearing the alarm leave the building immediately following the escape route and fire wardens and head to one of the meeting points Do not use the lifts Beyond first aid The Heidelberg University Hospital or Universit tsklinikum has a 24 hour accident and emergency facility and is at Im Neuenheimer Feld 672 It is a 8km or a 20 minute taxi ride away from EMBL In a medical emergency the ambulance service generally transports patients to this hospital Please remember to bring your own medication if needed to the conference Note that the next pharmacy is a 4 minute drive from the EMBL but for many medications you will be required to see a doctor to get a prescription Ensure in advance that your medical insurance will cover you during your visit in the event that you do need to see a doctor while in Heidelberg The EMBL course and conference office team are able to advise you on where to turn for emergency or non emergency medical treatment in Heidelberg and can assist you in making appointments and or arranging transport to get to the pharmacy or a doctor of your choice if necessary Please note we are not however permitted to personally accompany you off campus Sightseeing A variety of 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OverviewSpeakersProgrammePractical informationSponsorsMedia kit Overview Course overview This practical course will cover advanced light microscopy techniques, and participants will learn how to derive qualitative and quantitative insights into molecular mechanisms in cells, tissues and developing organisms. Guest researchers, together with microscopy experts from EMBL, will foster an intensive exchange of information through a balance of lectures and practical workshops. The focus of the course will be on the use of fluorescence microscopy to obtain information about the localisation and functions of proteins and other biomolecules at different spatial scales. Audience This course is directed towards researchers in the life sciences with access to light microscopy equipment, who have an immediate need to apply the techniques they will learn to solve biological problems at the cellular...
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