Structure and function of RNA-protein complexes
U5 snRNP is one of the key building blocks of the spliceosome, but how it is assembled and recycled remains unknown. In our new paper published in NSMB, we provide new mechanistic insights into this process. https://www.nature.com/articles/s41594-024-01250-5 We investigated the role of the assembly…
We are happy to share a new collaborative work lead by Jana Aupic and Alessandra Magistrato from the National Research Council of Italy (CNR) in Triest. Molecular dynamics simulations of the complete yeast catalytic spliceosome revealed detailed trajectories of the reagents during the first step of…
We are happy to share that our newest collaborative paper on self-damage control and defence system during pathogen infection was just published in Science! Huge congratulations to everyone involved, in particular our collaborators Daniel Fisch and Eva Frickel (https://frickellab.com/) for driving…
Our latest collaboration with Sarah Offley and Alessandro Gardini group from the Wistar Institute provides new insights into the structure and function of the previously uncharacterized protein associated with the Integrator complex. We used functional genomics to show that INTS15 is a core…
Check out our latest review in Current Opinion in Structural Biology summarizing recent progress in mechanistic studies of the spliceosome.
Our brief summary of the recent progress in understanding the structure and function of the Integrator complex was just published in Transcription. Moritz M. Pfleiderer & Wojciech P. Galej (2022) Emerging insights into the function and structure of the Integrator complex. Transcription,…
https://www.science.org/doi/10.1126/science.abm4245
Our new paper on the structure of the human U2 snRNP was just published in Science ! In human cells protein-coding genetic information is punctuated with non-coding segments – Introns, which need to be removed from pre-mRNA by the spliceosome. U2 snRNP recognises a conserved position within…
Moritz Pfleiderer, the first PhD student in the Galej group, has successfully defended his thesis last Thursday. During the PhD, Moritz studied multi-subunit Integrator complex, which is involved in transcription attenuation and snRNAs 3′-end processing. His results were recently published in…
The latest #cryoEM structure from Nagai group at MRC LMB in Cambridge identifying a new spliceosome assembly intermediate (Ci complex) is now published in Molecular Cell. The paper contains the most complete and accurate depiction of the catalytic spliceosome to date and identifies a new functional…