Edit

X-rays reveal surprising shape of scaffolding protein

The shape of PDZK1, an important scaffolding protein in cells, is full of surprises

IMAGE: Manon Boschard
IMAGE: Manon Boschard

While bioinformatics tools had suggested that the four domains of PDZK1 would behave like beads on a string, moving around in a highly flexible manner, X-ray experiments now show that PDZK1 actually has a relatively well-defined L-shaped conformation with only moderate flexibility.

This discovery was made by a research team led by EMBL group leaders Dmitri Svergun and Christian Löw – who is also a group leader at the Centre for Structural Systems Biology (CSSB) in Hamburg, Germany. The journal Structure published their results on 13 September 2018.

Scaffolding proteins like PDZK1 mediate interactions between proteins on the membranes of human cells. Löw, Svergun and colleagues performed small-angle X-ray scattering (SAXS) studies at DESY’s X-ray light source PETRA III to obtain the new information on the shapes and arrangement of PDKZK1’s domains, as well as on their interactions.

Tags: Hamburg, Löw, PDZK1, Research highlights, SAXS, Svergun, X-ray

More from this category

Picture of the week

To study the effect of commonly used drugs on bacterial envelopes, EMBL scientists applied a biochemical assay using a colour reaction. The deeper the red, the stronger the disruptive effect of the drug.

By  Marius Bruer

A metal rack holding glass test tubes with yellow and red solutions in them.

EMBLetc.

Read the latest Issues of our magazine - EMBLetc.

Looking for past print editions of EMBLetc.? Browse our archive, going back 20 years.

EMBLetc. archive
Edit