Protein Expression and Purification Core Facility

PEPCF expresses proteins in bacteria, insect and mammalian cells and uses a variety of chromatographic and biophysical techniques for protein purification and characterization.


Our mission is to provide scientific advice and assistance regarding all matters related to protein expression, purification and characterization.

The Protein Expression and Purification Core Facility (PEPCF) performs protein expression in E. coli, insect cells and mammalian cells.

For the protein purification we make use of a large variety of chromatographic techniques such as affinity chromatography, ion exchange and hydrophobic interaction chromatography and size exclusion chromatography.

The biophysics platform of the facility is dedicated to the characterization of proteins and their interactions with different types of molecules.

More detailed information about the PEPCF services and how to access the facility can be found in our guidelines. If you can’t find the information you are looking for, you are always welcome to contact the facility staff.

Strategy and construct design

The Protein Expression and Purification Core Facility can assist you in deciding which host organism to use for the protein expression and provide support for the design of the most optimal expression construct (i.e. plasmid backbone, protein tags, localization signals etc.) and cloning strategy.

Protein Expression

This section gives an overview of the different methods and strains that we are using for protein expression in E. coli, insect cells and mammalian cells.

Protein purification

The goal of the protein purification process is to obtain highly pure, stable and active protein for downstream experiments. The exact nature of the downstream applications will determine the purity level you need to obtain, the compatible buffer/storage conditions and the necessary quality control tests. In this section you can find some more information about the different steps in the protein purification workflow.

Biophysical support

The biophysics platform provides support for the design, execution and data analysis of biophysical and biochemical experiments aimed at the characterization of proteins, multi-subunit protein and protein-nucleic acid complexes and the interactions between proteins and other types of molecules.

Depending on the individual needs, the experiments can be performed by the PEPCF scientific officer for biophysical support or the users can be trained to operate the equipment independently and perform their own experiments.

General workflow for protein expression and purification experiments