Protein Expression and Purification Core Facility

PEPCF expresses proteins in bacteria, insect and mammalian cells and uses a variety of chromatographic and biophysical techniques for protein purification and characterization.

The facility expresses and purifies proteins from E. coli, insect cells and mammalian cells. For the recombinant protein purifications we implement a large array of chromatographic techniques. We also offer biophysical support for protein quality control, protein characterization and various types of interaction studies.


We strive to stay up-to-date with the latest scientific developments and trends in the field and test out new products and protocols for our users. 

We also maintain an extensive collection of expression vectors and bacterial strains and we have various frequently used proteins such as for example proteases and polymerases readily available.

  • Screening of expression constructs in different E. coli strains and culturing conditions using our automated liquid handling platform
  • Baculovirus-mediated protein expression in insect cells
  • Protein expression in mammalian suspension cultures
  • Purification of recombinant proteins using a variety of chromatographic techniques
  • Protein quality control
  • Characterization of proteins, protein complexes and the interactions of proteins with various types of molecules using biophysical techniques
  • Teaching and training of users and visitors in all aspects of protein expression, purification and biophysical characterization techniques

Reagent requests

We maintain a large database of expression vectors at the EMBL Protein Expression and Purification Core Facility. Vectors that are commercially available can only be shared with internal EMBL users, but vectors generated at EMBL are freely available to the entire academic research community via a Material Transfer Agreement. Vectors that can be shared with external users are indicated in yellow in the vector tables.

If you’re interested in obtaining reagents created at EMBL via the MTA, please complete the form and send a signed and dated pdf file to kim.remans@embl.de. Since the contracting partners are the institutes (not the scientists) the contract has to be signed by yourself and by an official representative of your institute who is authorized to sign binding agreements. Please understand that we are not in a position to negotiate changes of the wording in the text of our MTA.


We are open to collaborations with academic and industrial partners to assess new products or technological developments. Furthermore, we are an active member of a network of protein facilities across Europe called Protein Production and Purification Partnership in Europe (P4EU) to improve information exchange and the evaluation of novel technologies.

Community-wide experimental evaluation of the PROSS stability-design method.

Peleg Y, Vincentelli R, Collins BM, Chen KE, Livingstone EK, Weeratunga S, Leneva N, Guo Q, Remans K, Perez K, E K Bjerga G, Larsen Ø, Vaněk O, Skořepa O, Jacquemin S, Poterszman A, Kjaer S, Christodoulou E, Albeck S, Dym O, Ainbinder E, Unger T, Schuetz A, Matthes S, Bader M, de Marco A, Storici P, Semrau MS, Stolt-Bergner P, Aigner C, Suppmann S, Goldenzweig A, Fleishman SJ

Journal of molecular biology 2021



Protein Expression and Purification Core Facility

Phone: +49 6221 387- 8448
Fax: +49 6221 387- 8519

EMBL Heidelberg
Meyerhofstr. 1
69117 Heidelberg

15 Apr 2020

Producing proteins for coronavirus research

The Protein Expression and Purification Core Facility at EMBL Heidelberg will produce proteins for several coronavirus-related research projects, to assist the development of new strategies to fight t…