Publications – Protein Expression and Purification Core Facility

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Jumbo phage killer immune system targets early infection of nucleus-forming phages.

Yuping L, Guan L, Becher I, Makarova KS, Cao X, Hareendranath S, Guan J, Stein F, Yang S, Boergel A, Lapouge K, Remans K, Agard D, Savitski M, Typas A, Koonin EV, Feng Y, Bondy-Denomy J

Cell, 2025

doi:10.1016/j.cell.2025.02.016.

Structure of the nucleosome-bound human BCL7A.

Martin F, Kazrani AA, Lafouge J, Diaz-Jimenez DM, Siebert S, Fabbro-Burtschell L, Maillard E, Lapouge K, Mertens HDT, Sauter C, Leitner A, Ochsenbein F, Blais A, Bergamin E

Nucleic acids research, 2025

doi:10.1093/nar/gkaf273.

Time course and determinants of the antibody response to SARS-CoV-2 in Costa Rica: the RESPIRA study.

Herrero R, Fantin R, Loría V, Aparicio A, Prevots DR, Zúñiga M, Wong R, Morera M, Butt J, Binder M, Abdelnour A, Calderón A, Castro R, Cortes B, Ocampo R, Vanegas JC, Gail MH, Pfeiffer RM, Flock J, Remans K, Eberhardt L, Rastgou S, Magalhaes V, Porras C, Hildesheim A, Waterboer T, RESPIRA study group

BMC infectious diseases, 2025

doi:10.1186/s12879-025-10742-8.

TR-FRET assay for profiling HDAC10 inhibitors and PROTACs.

Remans K, Sehr P, Steimbach RR, Gunkel N, Miller AK

Methods in enzymology, 2025

doi:10.1016/bs.mie.2025.01.061.

The Drosophila RNA binding protein Hrp48 binds a specific RNA sequence of the msl-2 mRNA 3' UTR to regulate translation.

Lomoschitz A, Meyer J, Guitart T, Krepl M, Lapouge K, Hayn C, Schweimer K, Simon B, Šponer J, Gebauer F, Hennig J

Biophysical chemistry, 2025

doi:10.1016/j.bpc.2024.107346.

The molecular dissection of TRIM25's RNA-binding mechanism provides key insights into its antiviral activity.

Álvarez L, Haubrich K, Iselin L, Gillioz L, Ruscica V, Lapouge K, Augsten S, Huppertz I, Choudhury NR, Simon B, Masiewicz P, Lethier M, Cusack S, Rittinger K, Gabel F, Leitner A, Michlewski G, Hentze MW, Allain FHT, Castello A, Hennig J

Nature communications, 2024

doi:10.1038/s41467-024-52918-x.

Trim66's paternal deficiency causes intrauterine overgrowth.

Mielnicka M, Tabaro F, Sureka R, Acurzio B, Paoletti R, Scavizzi F, Raspa M, Crevenna AH, Lapouge K, Remans K, Boulard M

Life science alliance, 2024

doi:10.26508/lsa.202302512.

Revised whole genome and DNA methylome of Mycobacterium marinum type strain ATCC 927T.

Savijoki K, Deptula P, Roberts RJ, Hammarén M, Yli-Kauhaluoma J, Varmanen P, Parikka M

Microbiology resource announcements, 2024

doi:10.1128/mra.01016-23.

Tropomyosin 1-I/C coordinates kinesin-1 and dynein motors during oskar mRNA transport.

Heber S, McClintock MA, Simon B, Mehtab E, Lapouge K, Hennig J, Bullock SL, Ephrussi A

Nature structural & molecular biology, 2024

doi:10.1038/s41594-024-01212-x.

The use of baculovirus-mediated gene expression in mammalian cells for recombinant protein production.

Flock J, Xie Y, Lemaitre R, Lapouge K, Remans K

Methods in molecular biology (Clifton, N.J.), 2024

doi:10.1007/978-1-0716-3878-1_3.

Structural basis of RNA-induced autoregulation of the DExH-type RNA helicase maleless.

Jagtap PKA, Müller M, Kiss AE, Thomae AW, Lapouge K, Beck M, Becker PB, Hennig J

Molecular cell, 2023

doi:10.1016/j.molcel.2023.10.026.

A concise guide to choosing suitable gene expression systems for recombinant protein production.

Schütz A, Bernhard F, Berrow N, Buyel JF, Ferreira-da-Silva F, Haustraete J, van den Heuvel J, Hoffmann JE, de Marco A, Peleg Y, Suppmann S, Unger T, Vanhoucke M, Witt S, Remans K

STAR protocols, 2023

doi:10.1016/j.xpro.2023.102572.

Estimating the cumulative incidence of SARS-CoV-2 infection in Costa Rica: modelling seroprevalence data in a population-based cohort.

Fantin R, Agarwala N, Aparicio A, Pfeiffer R, Waterboer T, Abdelnour A, Butt J, Flock J, Remans K, Prevots DR, Porras C, Hildesheim A, Loria V, Gail MH, Herrero R, RESPIRA Study Group

Lancet regional health. Americas, 2023

doi:10.1016/j.lana.2023.100616.

In vitro and ex vivo proteomics of Mycobacterium marinum biofilms and the development of biofilm-binding synthetic nanobodies.

Hammarén MM, Luukinen H, Sillanpää A, Remans K, Lapouge K, Custódio T, Löw C, Myllymäki H, Montonen T, Seeger M, Robertson J, Nyman TA, Savijoki K, Parikka M

mSystems, 2023

doi:10.1128/msystems.01073-22.

Immunodominant surface epitopes power immune evasion in the African trypanosome.

Gkeka A, Aresta-Branco F, Triller G, Vlachou EP, van Straaten M, Lilic M, Olinares PDB, Perez K, Chait BT, Blatnik R, Ruppert T, Verdi JP, Stebbins CE, Papavasiliou FN

Cell reports, 2023

doi:10.1016/j.celrep.2023.112262.

In vitro and in cellulo ApoE particle formation, isolation, and characterization.

Lindner K, van Ek LC, Awwad K, Hanke F, Korepanova AV, Rybin V, Beckenbauer K, Gavin AC

STAR protocols, 2022

doi:10.1016/j.xpro.2022.101894.

Rational design of the zonulin inhibitor AT1001 derivatives as potential anti SARS-CoV-2.

Di Micco S, Rahimova R, Sala M, Scala MC, Vivenzio G, Musella S, Andrei G, Remans K, Mammri L, Snoeck R, Bifulco G, Di Matteo F, Vestuto V, Campiglia P, Márquez JA, Fasano A

European journal of medicinal chemistry, 2022

doi:10.1016/j.ejmech.2022.114857.

Extended N-terminal acetyltransferase Naa50 in filamentous fungi adds to Naa50 diversity.

Weidenhausen J, Kopp J, Ruger-Herreros C, Stein F, Haberkant P, Lapouge K, Sinning I

International journal of molecular sciences, 2022

doi:10.3390/ijms231810805.

Protein purification strategies must consider downstream applications and individual biological characteristics.

Remans K, Lebendiker M, Abreu C, Maffei M, Sellathurai S, May MM, Vaněk O, de Marco A

Microbial cell factories, 2022

doi:10.1186/s12934-022-01778-5.

Isoform- and cell-state-specific lipidation of ApoE in astrocytes.

Lindner K, Beckenbauer K, van Ek LC, Titeca K, de Leeuw SM, Awwad K, Hanke F, Korepanova AV, Rybin V, van der Kam EL, Mohler EG, Tackenberg C, Lakics V, Gavin AC

Cell reports, 2022

doi:10.1016/j.celrep.2022.110435.

Protein abundance and folding rather than the redox state of Kelch13 determine the artemisinin susceptibility of Plasmodium falciparum.

Schumann R, Bischoff E, Klaus S, Möhring S, Flock J, Keller S, Remans K, Ganter M, Deponte M

Redox biology, 2021

doi:10.1016/j.redox.2021.102177.

Deamidation drives molecular aging of the SARS-CoV-2 spike protein receptor-binding motif.

Lorenzo R, Defelipe LA, Aliperti L, Niebling S, Custódio TF, Löw C, Schwarz JJ, Remans K, Craig PO, Otero LH, Klinke S, García-Alai M, Sánchez IE, Alonso LG

The Journal of biological chemistry, 2021

doi:10.1016/j.jbc.2021.101175.

Structure and dynamics of the quaternary hunchback mRNA translation repression complex.

Macošek J, Simon B, Linse JB, Jagtap PKA, Winter SL, Foot J, Lapouge K, Perez K, Rettel M, Ivanović MT, Masiewicz P, Murciano B, Savitski MM, Loedige I, Hub JS, Gabel F, Hennig J

Nucleic acids research, 2021

doi:10.1093/nar/gkab635.

Molecular basis of mRNA transport by a kinesin-1-atypical tropomyosin complex.

Dimitrova-Paternoga L, Jagtap PKA, Cyrklaff A, Vaishali, Lapouge K, Sehr P, Perez K, Heber S, Löw C, Hennig J, Ephrussi A

Genes & development, 2021

doi:10.1101/gad.348443.121.

Dynamic, variable oligomerization and the trafficking of variant surface glycoproteins of Trypanosoma brucei.

Umaer K, Aresta-Branco F, Chandra M, van Straaten M, Zeelen J, Lapouge K, Waxman B, Stebbins CE, Bangs JD

Traffic (Copenhagen, Denmark), 2021

doi:10.1111/tra.12806.

Quality control of protein reagents for the improvement of research data reproducibility.

de Marco A, Berrow N, Lebendiker M, Garcia-Alai M, Knauer SH, Lopez-Mendez B, Matagne A, Parret A, Remans K, Uebel S, Raynal B

Nature communications, 2021

doi:10.1038/s41467-021-23167-z.

Quality control of purified proteins to improve data quality and reproducibility: results from a large-scale survey.

Berrow N, de Marco A, Lebendiker M, Garcia-Alai M, Knauer SH, Lopez-Mendez B, Matagne A, Parret A, Remans K, Uebel S, Raynal B

European biophysics journal : EBJ, 2021

doi:10.1007/s00249-021-01528-2.

Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study.

López-Méndez B, Baron B, Brautigam CA, Jowitt TA, Knauer SH, Uebel S, Williams MA, Sedivy A, Abian O, Abreu C, Adamczyk M, Bal W, Berger S, Buell AK, Carolis C, Daviter T, Fish A, Garcia-Alai M, Guenther C, Hamacek J, Holková J, Houser J, Johnson C, Kelly S, Leech A, Mas C, Matulis D, McLaughlin SH, Montserret R, Nasreddine R, Nehmé R, Nguyen Q, Ortega-Alarcón D, Perez K, Pirc K, Piszczek G, Podobnik M, Rodrigo N, Rokov-Plavec J, Schaefer S, Sharpe T, Southall J, Staunton D, Tavares P, Vanek O, Weyand M, Wu D

European biophysics journal : EBJ, 2021

doi:10.1007/s00249-021-01532-6.

Community-wide experimental evaluation of the PROSS stability-design method.

Peleg Y, Vincentelli R, Collins BM, Chen KE, Livingstone EK, Weeratunga S, Leneva N, Guo Q, Remans K, Perez K, E K Bjerga G, Larsen Ø, Vaněk O, Skořepa O, Jacquemin S, Poterszman A, Kjaer S, Christodoulou E, Albeck S, Dym O, Ainbinder E, Unger T, Schuetz A, Matthes S, Bader M, de Marco A, Storici P, Semrau MS, Stolt-Bergner P, Aigner C, Suppmann S, Goldenzweig A, Fleishman SJ

Journal of molecular biology, 2021

doi:10.1016/j.jmb.2021.166964.

Fast and inexpensive whole-genome sequencing library preparation from intact yeast cells.

Vonesch SC, Li S, Szu Tu C, Hennig BP, Dobrev N, Steinmetz LM

G3 (Bethesda, Md.), 2021

doi:10.1093/g3journal/jkaa009.

Structure of trypanosome coat protein VSGsur and function in suramin resistance.

Zeelen J, van Straaten M, Verdi J, Hempelmann A, Hashemi H, Perez K, Jeffrey PD, Hälg S, Wiedemar N, Mäser P, Papavasiliou FN, Stebbins CE

Nature microbiology, 2021

doi:10.1038/s41564-020-00844-1.

Extensive OMICS resource for Sf21 and Tni cell lines.

Galik B, Landry JM, Kirkpatrick J, Fritz M, Baying B, Blake J, Haase B, Collier PG, Hercog R, Pavlinic D, Stolt-Bergner P, Besir H, Remans K, Gyenesei A, Benes V

2021

doi:10.1101/2021.04.06.438574.

Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2.

Custódio TF, Das H, Sheward DJ, Hanke L, Pazicky S, Pieprzyk J, Sorgenfrei M, Schroer MA, Gruzinov AY, Jeffries CM, Graewert MA, Svergun DI, Dobrev N, Remans K, Seeger MA, McInerney GM, Murrell B, Hällberg BM, Löw C

Nature communications, 2020

doi:10.1038/s41467-020-19204-y.

An HPF1/PARP1-based chemical biology strategy for exploring ADP-ribosylation.

Bonfiglio JJ, Leidecker O, Dauben H, Longarini EJ, Colby T, San Segundo-Acosta P, Perez KA, Matic I

Cell, 2020

doi:10.1016/j.cell.2020.09.055.

Dodecin as carrier protein for immunizations and bioengineering applications.

Bourdeaux F, Kopp Y, Lautenschläger J, Gößner I, Besir H, Vabulas RM, Grininger M

Scientific reports, 2020

doi:10.1038/s41598-020-69990-0.

Substitutions of residues in a loop surrounding the active site of a P-I snake venom metalloproteinase abrogates its hemorrhagic activity.

Camacho E, Rucavado A, Escalante T, Gutiérrez JM, Remans K

Toxicon : official journal of the International Society on Toxinology, 2020

doi:10.1016/j.toxicon.2019.12.123.

McQ – an open-source multiplexed SARS-CoV-2 quantification platform.

Vonesch SC, Bredikhin D, Dobrev N, Villacorta L, Kleinendorst R, Cacace E, Flock J, Frank M, Jung F, Kornienko J, Mitosch K, Osuna-Lopez M, Zimmermann J, Goettig S, Hamprecht A, Kraeusslich H, Knop M, Typas A, Steinmetz LM, Benes V, Remans K, Krebs AR

medRxiv, 2020

doi:10.1101/2020.12.02.20242628.

Structure-based screening of binding affinities via small-angle X-ray scattering.

Chen PC, Masiewicz P, Perez K, Hennig J

2020

doi:10.1107/S2052252520004169.

Quality control of purified proteins to improve research data reproducibility.

Uebel S, de Marco A, Berrow N, Lebendiker M, Garcia-Alai M, Knauer SH, Lopez-Mendez B, Matagne A, Parret A, Remans K, Raynal B

Journal of biomolecular techniques : JBT, 2019

doi:.

Site mutation of residues in a loop surrounding the active site of a PI snake venom metalloproteinase abrogates its hemorrhagic activity.

Camacho E, Escalante T, Remans K, Gutiérrez JM, Rucavado A

Biochemical and biophysical research communications, 2019

doi:10.1016/j.bbrc.2019.03.152.

Baculovirus-driven protein expression in insect cells: a benchmarking study.

Stolt-Bergner P, Benda C, Bergbrede T, Besir H, Celie PHN, Chang C, Drechsel D, Fischer A, Geerlof A, Giabbai B, van den Heuvel J, Huber G, Knecht W, Lehner A, Lemaitre R, Nordén K, Pardee G, Racke I, Remans K, Sander A, Scholz J, Stadnik M, Storici P, Weinbruch D, Zaror I, Lua LHL, Suppmann S

Journal of structural biology, 2018

doi:10.1016/j.jsb.2018.03.004.

A general small-angle X-ray scattering-based screening protocol validated for protein-RNA interactions.

Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J

ACS combinatorial science, 2018

doi:10.1021/acscombsci.8b00007.

Large-scale low-cost NGS library preparation using a robust Tn5 purification and tagmentation protocol.

Hennig BP, Velten L, Racke I, Szu Tu C, Thoms M, Rybin V, Besir H, Remans K, Steinmetz LM

G3 (Bethesda, Md.), 2018

doi:10.1534/g3.117.300257.

Structural Basis for a Safety-Belt Mechanism That Anchors Condensin to Chromosomes.

Kschonsak M, Merkel F, Bisht S, Metz J, Rybin V, Hassler M, Haering CH

Cell, 2017

doi:10.1016/j.cell.2017.09.008.

Intermolecular base stacking mediates RNA-RNA interaction in a crystal structure of the RNA chaperone Hfq

Schulz EC, Seiler M, Zuliani C, Voigt F, Rybin V, Pogenberg V, Mücke N, Wilmanns M, Gibson TJ, Barabas O.

2017

doi:10.1038/s41598-017-10085-8.

A genome scan for milk production traits in dairy goats reveals two new mutations in Dgat1 reducing milk fat content.

Martin P, Palhière I, Maroteau C, Bardou P, Canale-Tabet K, Sarry J, Woloszyn F, Bertrand-Michel J, Racke I, Besir H, Rupp R, Tosser-Klopp G

Scientific reports, 2017

doi:10.1038/s41598-017-02052-0.

A Generic Protocol for Purifying Disulfide-Bonded Domains and Random Protein Fragments Using Fusion Proteins with SUMO3 and Cleavage by SenP2 Protease.

Besir H

Methods in molecular biology (Clifton, N.J.), 2017

doi:10.1007/978-1-4939-6887-9_9.

A bromodomain-DNA interaction facilitates acetylation-dependent bivalent nucleosome recognition by the BET protein BRDT

Miller TC, Simon B, Rybin V, Grötsch H, Curtet S, Khochbin S, Carlomagno T, Müller CW

NATURE COMMUNICATIONS, 2016

doi:10.1038/ncomms13855.

Genetic code expansion for multiprotein complex engineering.

Koehler C, Sauter PF, Wawryszyn M, Girona GE, Gupta K, Landry JJ, Fritz MH, Radic K, Hoffmann JE, Chen ZA, Zou J, Tan PS, Galik B, Junttila S, Stolt-Bergner P, Pruneri G, Gyenesei A, Schultz C, Biskup MB, Besir H, Benes V, Rappsilber J, Jechlinger M, Korbel JO, Berger I, Braese S, Lemke EA

Nature methods, 2016

doi:10.1038/nmeth.4032.

Using the fluorescent properties of STO-609 as a tool to assist structure-function analyses of recombinant CaMKK2

Gerner L, Munack S, Temmerman K, Lawrence-Dærner A, Besir H, Wilmanns M, Jensen JK, Thiede B, Mills IG, Morth JP

2016

doi:10.1016/j.bbrc.2016.05.045.

Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli.

Gerner L, Munack S, Temmerman K, Lawrence-Dörner AM, Besir H, Wilmanns M, Jensen JK, Thiede B, Mills IG, Morth JP

Data in brief, 2016

doi:10.1016/j.dib.2016.06.033.

Critical reflections on synthetic gene design for recombinant protein expression.

Parret AH, Besir H, Meijers R

Current opinion in structural biology, 2016

doi:10.1016/j.sbi.2016.07.004.

Lipid Cooperativity as a General Membrane-Recruitment Principle for PH Domains.

Vonkova I, Saliba AE, Deghou S, Anand K, Ceschia S, Doerks T, Galih A, Kugler KG, Maeda K, Rybin V, van Noort V, Ellenberg J, Bork P, Gavin AC

Cell reports, 2015

doi:10.1016/j.celrep.2015.07.054.

Genomic Analysis and Isolation of RNA Polymerase II Dependent Promoters from Spodoptera frugiperda

Bleckmann M, Fritz MHY, Bhuju S, Jarek M, Schurig M, Geffers R, Benes V, Besir H, van den Heuvel J

PLOS ONE, 2015

doi:10.1371/journal.pone.0132898.

The crystal structure of the Drosophila germline inducer oskar identifies two domains with distinct Vasa helicase- and RNA-binding activities.

Jeske M, Bordi M, Glatt S, Müller S, Rybin V, Müller CW, Ephrussi A

Cell Reports, 2015

doi:10.1016/j.celrep.2015.06.055.

An organized co-assembly of clathrin adaptors is essential for endocytosis.

Skruzny M, Desfosses A, Prinz S, Dodonova SO, Gieras A, Uetrecht C, Jakobi AJ, Abella M, Hagen WJ, Schulz J, Meijers R, Rybin V, Briggs JA, Sachse C, Kaksonen M

Developmental cell, 2015

doi:10.1016/j.devcel.2015.02.023.

Structure of the Kti11/Kti13 Heterodimer and Its Double Role in Modifications of tRNA and Eukaryotic Elongation Factor 2.

Glatt S, Zabel R, Vonkova I, Kumar A, Netz DJ, Pierik AJ, Rybin V, Lill R, Gavin A, Balbach J, Breunig KD, Müller CW

Structure (London, England : 1993), 2015

doi:10.1016/j.str.2014.11.008.

Crystal structures of the phosphorylated BRI1 kinase domain and implications for brassinosteroid signal initiation.

Bojar D, Martinez J, Santiago J, Rybin V, Bayliss R, Hothorn M

The Plant journal : for cell and molecular biology, 2014

doi:10.1111/tpj.12445.

The novel Fh8 and H fusion partners for soluble protein expression in Escherichia coli: a comparison with the traditional gene fusion technology

Costa SJ, Almeida A, Castro A, Domingues L, Besir H

Applied microbiology and biotechnology, 2013

doi:10.1007/s00253-012-4559-1.

Structural basis of histone H2A-H2B recognition by the essential chaperone FACT

Hondele M, Stuwe T, Hassler M, Halbach F, Bowman A, Zhang ET, Nijmeijer B, Kotthoff C, Rybin V, Amlacher S, Hurt E, Ladurner AG

Nature, 2013

doi:10.1038/nature12242.

Structural and functional characterization of a phosphatase domain within yeast general transcription factor IIIC

Taylor NM, Glatt S, Hennrich ML, von Scheven G, Grötsch H, Fernández-Tornero C, Rybin V, Gavin AC, Kolb P, Müller CW

The Journal of biological chemistry, 2013

doi:10.1074/jbc.m112.427856.

A family of macrodomain proteins reverses cellular mono-ADP-ribosylation

Jankevicius G, Hassler M, Golia B, Rybin V, Zacharias M, Timinszky G, Ladurner AG

Nature structural & molecular biology, 2013

doi:10.1038/nsmb.2523.

A new method to customize protein expression vectors for fast, efficient and background free parallel cloning

Scholz J, Besir H, Strasser C, Suppmann S

BMC biotechnology, 2013

doi:10.1186/1472-6750-13-12.

Use of the uteroglobin platform for the expression of a bivalent antibody against oncofetal fibronectin in Escherichia coli

Ventura E, Riondato M, Sambuceti G, Salis A, Damonte G, Cordazzo C, Besir H, Pistoia V, Zardi L

PloS one, 2013

doi:10.1371/journal.pone.0082878.

Solution structure analysis of the HPV16 E6 oncoprotein reveals a self-association mechanism required for E6-mediated degradation of p53

Zanier K, ould M'hamed ould Sidi A, Boulade-Ladame C, Rybin V, Chappelle A, Atkinson A, Kieffer B, Travé G

Structure (London, England : 1993), 2012

doi:10.1016/j.str.2012.02.001.

GAGE cancer-germline antigens are recruited to the nuclear envelope by germ cell-less (GCL)

Gjerstorff MF, Rösner HI, Pedersen CB, Greve KB, Schmidt S, Wilson KL, Mollenhauer J, Besir H, Poulsen FM, Møllegaard NE, Ditzel HJ

PloS one, 2012

doi:10.1371/journal.pone.0045819.

The protein interaction network of a taxis signal transduction system in a halophilic archaeon

Schlesner M, Miller A, Besir H, Aivaliotis M, Streif J, Scheffer B, Siedler F, Oesterhelt D

BMC microbiology, 2012

doi:10.1186/1471-2180-12-272.

Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium

van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC

Molecular systems biology, 2012

doi:10.1038/msb.2012.4.

Characterization of an antifreeze protein from the polar diatom Fragilariopsis cylindrus and its relevance in sea ice

Bayer-Giraldi M, Weikusat I, Besir H, Dieckmann G

Cryobiology, 2011

doi:10.1016/j.cryobiol.2011.08.006.

The Xist RNA A-repeat comprises a novel AUCG tetraloop fold and a platform for multimerization

Duszczyk MM, Wutz A, Rybin V, Sattler M

RNA (New York, N.Y.), 2011

doi:10.1261/rna.2747411.

Multi-domain conformational selection underlies pre-mRNA splicing regulation by U2AF

Mackereth CD, Madl T, Bonnal S, Simon B, Zanier K, Gasch A, Rybin V, Valcárcel J, Sattler M

Nature, 2011

doi:10.1038/nature10171.

Chromatin-modifying complex component Nurf55/p55 associates with histones H3 and H4 and polycomb repressive complex 2 subunit Su(z)12 through partially overlapping binding sites

Nowak AJ, Alfieri C, Stirnimann CU, Rybin V, Baudin F, Ly-Hartig N, Lindner D, Müller CW

The Journal of biological chemistry, 2011

doi:10.1074/jbc.m110.207407.

Insights into the function of the CRM1 cofactor RanBP3 from the structure of its Ran-binding domain

Langer K, Dian C, Rybin V, Müller CW, Petosa C

PloS one, 2011

doi:10.1371/journal.pone.0017011.

A systematic screen for protein-lipid interactions in Saccharomyces cerevisiae.

Gallego O, Betts MJ, Gvozdenovic-Jeremic J, Maeda K, Matetzki C, Aguilar-Gurrieri C, Beltran-Alvarez P, Bonn S, Fernández-Tornero C, Jensen LJ, Kuhn M, Trott J, Rybin V, Müller CW, Bork P, Kaksonen M, Russell RB, Gavin AC

Molecular systems biology, 2010

doi:10.1038/msb.2010.87.

Expression, purification and characterization of the cancer-germline antigen GAGE12I: a candidate for cancer immunotherapy

Gjerstorff MF, Besir H, Larsen MR, Ditzel HJ

Protein expression and purification, 2010

doi:10.1016/j.pep.2010.05.010.

Structural insights into the pH-controlled targeting of plant cell-wall invertase by a specific inhibitor protein

Hothorn M, Van den Ende W, Lammens W, Rybin V, Scheffzek K

Proceedings of the National Academy of Sciences of the United States of America, 2010

doi:10.1073/pnas.1004481107.

Structure and function of the hetero-oligomeric cysteine synthase complex in plants

Wirtz M, Birke H, Heeg C, Müller C, Hosp F, Throm C, König S, Feldman-Salit A, Rippe K, Petersen G, Wade RC, Rybin V, Scheffzek K, Hell R

The Journal of biological chemistry, 2010

doi:10.1074/jbc.m110.157446.

Analysis of the specific interactions between the lectin domain of malectin and diglucosides

Schallus T, Fehér K, Sternberg U, Rybin V, Muhle-Goll C

Glycobiology, 2010

doi:10.1093/glycob/cwq059.

CHIP participates in protein triage decisions by preferentially ubiquitinating Hsp70-bound substrates

Stankiewicz M, Nikolay R, Rybin V, Mayer MP

The FEBS journal, 2010

doi:10.1111/j.1742-4658.2010.07737.x.

68Ga-labelled recombinant antibody variants for immuno-PET imaging of solid tumours

Eder M, Knackmuss S, Le Gall F, Reusch U, Rybin V, Little M, Haberkorn U, Mier W, Eisenhut M

European journal of nuclear medicine and molecular imaging, 2010

doi:10.1007/s00259-010-1392-6.

Regulation of oscillatory contraction in insect flight muscle by troponin

Krzic U, Rybin V, Leonard KR, Linke WA, Bullard B

Journal of molecular biology, 2010

doi:10.1016/j.jmb.2010.01.039.

E6 proteins from diverse papillomaviruses self-associate both in vitro and in vivo

Zanier K, Ruhlmann C, Melin F, Masson M, Ould M'hamed Ould Sidi A, Bernard X, Fischer B, Brino L, Ristriani T, Rybin V, Baltzinger M, Vande Pol S, Hellwig P, Schultz P, Travé G

Journal of molecular biology, 2010

doi:10.1016/j.jmb.2009.11.022.

Proteome organization in a genome-reduced bacterium

Kühner S, van Noort V, Betts MJ, Leo-Macias A, Batisse C, Rode M, Yamada T, Maier T, Bader S, Beltran-Alvarez P, Castaño-Diez D, Chen WH, Devos D, Güell M, Norambuena T, Racke I, Rybin V, Schmidt A, Yus E, Aebersold R, Herrmann R, Böttcher B, Frangakis AS, Russell RB, Serrano L, Bork P, Gavin AC

Science (New York, N.Y.), 2009

doi:10.1126/science.1176343.

Identification of unusual E6 and E7 proteins within avian papillomaviruses: cellular localization, biophysical characterization, and phylogenetic analysis

Van Doorslaer K, Sidi AO, Zanier K, Rybin V, Deryckère F, Rector A, Burk RD, Lienau EK, van Ranst M, Travé G

Journal of virology, 2009

doi:10.1128/jvi.01777-08.

Molecular recognition of histone lysine methylation by the Polycomb group repressor dSfmbt

Grimm C, Matos R, Ly-Hartig N, Steuerwald U, Lindner D, Rybin V, Müller J, Müller CW

The EMBO journal, 2009

doi:10.1038/emboj.2009.147.

Proteasomal and genetic inactivation of the NF1 tumor suppressor in gliomagenesis

McGillicuddy LT, Fromm JA, Hollstein PE, Kubek S, Beroukhim R, De Raedt T, Johnson BW, Williams SM, Nghiemphu P, Liau LM, Cloughesy TF, Mischel PS, Parret A, Seiler J, Moldenhauer G, Scheffzek K, Stemmer-Rachamimov AO, Sawyers CL, Brennan C, Messiaen L, Mellinghoff IK, Cichowski K

Cancer cell, 2009

doi:10.1016/j.ccr.2009.05.009.

Catalytic core of a membrane-associated eukaryotic polyphosphate polymerase

Hothorn M, Neumann H, Lenherr ED, Wehner M, Rybin V, Hassa PO, Uttenweiler A, Reinhardt M, Schmidt A, Seiler J, Ladurner AG, Herrmann C, Scheffzek K, Mayer A

Science (New York, N.Y.), 2009

doi:10.1126/science.1168120.

Effects of recombinant protein misfolding and aggregation on bacterial membranes

Ami D, Natalello A, Schultz T, Gatti-Lafranconi P, Lotti M, Doglia SM, de Marco A

Biochimica et biophysica acta, 2009

doi:10.1016/j.bbapap.2008.10.015.

Lipids regulate P2X7-receptor-dependent actin assembly by phagosomes via ADP translocation and ATP synthesis in the phagosome lumen

Kuehnel MP, Rybin V, Anand PK, Anes E, Griffiths G

Journal of cell science, 2009

doi:10.1242/jcs.034199.

Dimerization and protein binding specificity of the U2AF homology motif of the splicing factor Puf60

Corsini L, Hothorn M, Stier G, Rybin V, Scheffzek K, Gibson TJ, Sattler M

The Journal of biological chemistry, 2009

doi:10.1074/jbc.m805395200.

Staining of proteins in gels with Coomassie G-250 without organic solvent and acetic acid

Lawrence AM, Besir HU

Journal of visualized experiments : JoVE, 2009

doi:10.3791/1350.

The FACT Spt16 "peptidase" domain is a histone H3-H4 binding module

Stuwe T, Hothorn M, Lejeune E, Rybin V, Bortfeld M, Scheffzek K, Ladurner AG

Proceedings of the National Academy of Sciences of the United States of America, 2008

doi:10.1073/pnas.0712293105.

The redox switch of gamma-glutamylcysteine ligase via a reversible monomer-dimer transition is a mechanism unique to plants

Gromes R, Hothorn M, Lenherr ED, Rybin V, Scheffzek K, Rausch T

The Plant journal : for cell and molecular biology, 2008

doi:10.1111/j.1365-313x.2008.03477.x.

Decoding of methylated histone H3 tail by the Pygo-BCL9 Wnt signaling complex

Fiedler M, Sánchez-Barrena MJ, Nekrasov M, Mieszczanek J, Rybin V, Müller J, Evans P, Bienz M

Molecular cell, 2008

doi:10.1016/j.molcel.2008.03.011.

Physical and chemical perturbations induce the formation of protein aggregates with different structural features

Natalello A, Santarella R, Doglia SM, de Marco A

Protein expression and purification, 2008

doi:10.1016/j.pep.2007.12.002.

Muscle RING-finger protein-1 (MuRF1) as a connector of muscle energy metabolism and protein synthesis

Koyama S, Hata S, Witt CC, Ono Y, Lerche S, Ojima K, Chiba T, Doi N, Kitamura F, Tanaka K, Abe K, Witt SH, Rybin V, Gasch A, Franz T, Labeit S, Sorimachi H

Journal of molecular biology, 2008

doi:10.1016/j.jmb.2007.11.049.

Mutation analysis in Costello syndrome: functional and structural characterization of the HRAS p.Lys117Arg mutation

Denayer E, Parret A, Chmara M, Schubbert S, Vogels A, Devriendt K, Frijns JP, Rybin V, de Ravel TJ, Shannon K, Cools J, Scheffzek K, Legius E

Human mutation, 2008

doi:10.1002/humu.20616.

Structural and functional analyses of methyl-lysine binding by the malignant brain tumour repeat protein Sex comb on midleg

Grimm C, de Ayala Alonso AG, Rybin V, Steuerwald U, Ly-Hartig N, Fischle W, Müller J, Müller CW

EMBO reports, 2007

doi:10.1038/sj.embor.7401085.

Crystal structure of human filamin C domain 23 and small angle scattering model for filamin C 23-24 dimer.

Sjekloća L, Pudas R, Sjöblom B, Konarev P, Carugo O, Rybin V, Kiema TR, Svergun D, Ylänne J, Djinović Carugo K

Journal of molecular biology, 2007

doi:10.1016/j.jmb.2007.02.018.

Structural and biochemical characterization of the yeast exosome component Rrp40

Oddone A, Lorentzen E, Basquin J, Gasch A, Rybin V, Conti E, Sattler M

EMBO reports, 2007

doi:10.1038/sj.embor.7400856.

cAMP synthesis and degradation by phagosomes regulate actin assembly and fusion events: consequences for mycobacteria

Kalamidas SA, Kuehnel MP, Peyron P, Rybin V, Rauch S, Kotoulas OB, Houslay M, Hemmings BA, Gutierrez MG, Anes E, Griffiths G

Journal of cell science, 2006

doi:10.1242/jcs.03091.

HDAC6-p97/VCP controlled polyubiquitin chain turnover

Boyault C, Gilquin B, Zhang Y, Rybin V, Garman E, Meyer-Klaucke W, Matthias P, Müller CW, Khochbin S

The EMBO journal, 2006

doi:10.1038/sj.emboj.7601210.

YfhJ, a molecular adaptor in iron-sulfur cluster formation or a frataxin-like protein?

Pastore C, Adinolfi S, Huynen MA, Rybin V, Martin S, Mayer M, Bukau B, Pastore A

Structure (London, England : 1993), 2006

doi:10.1016/j.str.2006.02.010.

Mouse profilin 2 regulates endocytosis and competes with SH3 ligand binding to dynamin 1

Gareus R, Di Nardo A, Rybin V, Witke W

The Journal of biological chemistry, 2006

doi:10.1074/jbc.m503528200.

The evaluation of the factors that cause aggregation during recombinant expression in E. coli is simplified by the employment of an aggregation-sensitive reporter

Schultz T, Martinez L, de Marco A

Microbial cell factories, 2006

doi:10.1186/1475-2859-5-28.

Substrate access to the active sites in aminopeptidase T, a representative of a new metallopeptidase clan

Odintsov SG, Sabała I, Bourenkov G, Rybin V, Bochtler M

Journal of molecular biology, 2005

doi:10.1016/j.jmb.2005.09.042.

Nup155 regulates nuclear envelope and nuclear pore complex formation in nematodes and vertebrates

Franz C, Askjaer P, Antonin W, Iglesias CL, Haselmann U, Schelder M, de Marco A, Wilm M, Antony C, Mattaj IW

The EMBO journal, 2005

doi:10.1038/sj.emboj.7600825.

Staphylococcus aureus aminopeptidase S is a founding member of a new peptidase clan

Odintsov SG, Sabała I, Bourenkov G, Rybin V, Bochtler M

The Journal of biological chemistry, 2005

doi:10.1074/jbc.m502023200.

Native folding of aggregation-prone recombinant proteins in Escherichia coli by osmolytes, plasmid- or benzyl alcohol-overexpressed molecular chaperones

de Marco A, Vigh L, Diamant S, Goloubinoff P

Cell stress & chaperones, 2005

doi:10.1379/CSC-139R.1.

Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors

Dümmler A, Lawrence AM, de Marco A

Microbial cell factories, 2005

doi:10.1186/1475-2859-4-34.

The solubility and stability of recombinant proteins are increased by their fusion to NusA

De Marco V, Stier G, Blandin S, de Marco A

Biochemical and biophysical research communications, 2004

doi:10.1016/j.bbrc.2004.07.189.

Recombinant proteins fused to thermostable partners can be purified by heat incubation

de Marco A, Casatta E, Savaresi S, Geerlof A

Journal of biotechnology, 2004

doi:10.1016/j.jbiotec.2003.10.008.

Computer-aided design of a PDZ domain to recognize new target sequences

Reina J, Lacroix E, Hobson SD, Fernandez-Ballester G, Rybin V, Schwab MS, Serrano L, Gonzalez C

Nature structural biology, 2002

doi:10.1038/nsb815.

Phosphoinositides regulate membrane-dependent actin assembly by latex bead phagosomes

Defacque H, Bos E, Garvalov B, Barret C, Roy C, Mangeat P, Shin HW, Rybin V, Griffiths G

Molecular biology of the cell, 2002

doi:10.1091/mbc.01-06-0314.

Functional organization of the yeast proteome by systematic analysis of protein complexes

Gavin AC, Bösche M, Krause R, Grandi P, Marzioch M, Bauer A, Schultz J, Rick JM, Michon AM, Cruciat CM, Remor M, Höfert C, Schelder M, Brajenovic M, Ruffner H, Merino A, Klein K, Hudak M, Dickson D, Rudi T, Gnau V, Bauch A, Bastuck S, Huhse B, Leutwein C, Heurtier MA, Copley RR, Edelmann A, Querfurth E, Rybin V, Drewes G, Raida M, Bouwmeester T, Bork P, Seraphin B, Kuster B, Neubauer G, Superti-Furga G

Nature, 2002

doi:10.1038/415141a.

Functional synergy between Rab5 effector Rabaptin-5 and exchange factor Rabex-5 when physically associated in a complex

Lippé R, Miaczynska M, Rybin V, Runge A, Zerial M

Molecular biology of the cell, 2001

doi:10.1091/mbc.12.7.2219.

Vps9, Rabex-5 and DSS4: proteins with weak but distinct nucleotide-exchange activities for Rab proteins

Esters H, Alexandrov K, Iakovenko A, Ivanova T, Thomä N, Rybin V, Zerial M, Scheidig AJ, Goody RS

Journal of molecular biology, 2001

doi:10.1006/jmbi.2001.4735.

ATP-dependent membrane assembly of F-actin facilitates membrane fusion

Jahraus A, Egeberg M, Hinner B, Habermann A, Sackman E, Pralle A, Faulstich H, Rybin V, Defacque H, Griffiths G

Molecular biology of the cell, 2001

doi:10.1091/mbc.12.1.155.

The Eps8 protein coordinates EGF receptor signalling through Rac and trafficking through Rab5

Lanzetti L, Rybin V, Malabarba MG, Christoforidis S, Scita G, Zerial M, Di Fiore PP

Nature, 2000

doi:10.1038/35042605.

Crystal structure of the protein disulfide bond isomerase, DsbC, from Escherichia coli

McCarthy AA, Haebel PW, Törrönen A, Rybin V, Baker EN, Metcalf P

Nature structural biology, 2000

doi:10.1038/73295.

Oligomeric complexes link Rab5 effectors with NSF and drive membrane fusion via interactions between EEA1 and syntaxin 13

McBride HM, Rybin V, Murphy C, Giner A, Teasdale R, Zerial M

Cell, 1999

doi:10.1016/s0092-8674(00)81966-2.

Two distinct effectors of the small GTPase Rab5 cooperate in endocytic membrane fusion

Gournier H, Stenmark H, Rybin V, Lippé R, Zerial M

The EMBO journal, 1998

doi:10.1093/emboj/17.7.1930.

Distinct Rab-binding domains mediate the interaction of Rabaptin-5 with GTP-bound Rab4 and Rab5

Vitale G, Rybin V, Christoforidis S, Thornqvist P, McCaffrey M, Stenmark H, Zerial M

The EMBO journal, 1998

doi:10.1093/emboj/17.7.1941.

A novel Rab5 GDP/GTP exchange factor complexed to Rabaptin-5 links nucleotide exchange to effector recruitment and function

Horiuchi H, Lippé R, McBride HM, Rubino M, Woodman P, Stenmark H, Rybin V, Wilm M, Ashman K, Mann M, Zerial M

Cell, 1997

doi:10.1016/s0092-8674(00)80380-3.

Endosome dynamics regulated by a Rho protein

Murphy C, Saffrich R, Grummt M, Gournier H, Rybin V, Rubino M, Auvinen P, Lütcke A, Parton RG, Zerial M

Nature, 1996

doi:10.1038/384427a0.

GTPase activity of Rab5 acts as a timer for endocytic membrane fusion

Rybin V, Ullrich O, Rubino M, Alexandrov K, Simon I, Seabra MC, Goody R, Zerial M

Nature, 1996

doi:10.1038/383266a0.