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Proteomics Core Facility

This facility provides a full proteomics infrastructure for the identification and characterisation of proteins.

Instrumentation and Software

Sample preparation

KingFisher Apex
(image: ThermoFisher Scientific)

KingFisher Apex

for semi-automated processing of samples.

Sample clean-up via the SP3 workflow (Hughes et al., 2019, PMID: 30464214)
Fe-IMAC enrichment of phosphorylated peptides (Leutert et al., 2019, PMID: 31885202)

Liquid chromatography

Offline HPLC systems

Online HPLC systems

Ultimate 3000 nano HPLC (image: ThermoFisher Scientific)

Vanquish Neo nano HPLC (image: ThermoFisher scientific)

1200 Infinity (Agilent)

high pH reversed phase chromatography
peptide size-exclusion chromatography

Ultimate 3000 (Dionex)

Ultimate 3000 (Dionex)

reverse phase C18 chromatography for online fractionation of peptides prior to mass spectrometry

Vanquish Neo (Thermo Scientific)

reverse phase C18 chromatography for online fractionation of peptides prior to mass spectrometry

Mass spectrometry

QExactive Plus

Q Exactive Plus
(image: ThermoFisher Scientific)

Orbitrap Tribrid Lumos

Orbitrap Tribrid Lumos
(image: ThermoFisher Scientific)

Orbitrap Exploris 480

Orbitrap Exploris 480
(image: ThermoFisher Scientific)

Orbitrap Astral

Orbitrap Astral (image: ThermoFisher Scientific)

Software

MSFragger/FragPipe
DIA-NN
pLink3
MaxQuant
Skyline
R for statistical analysis and data visualization

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