We accept samples for protein identification from coomassie and silverstained gels.
Not all coomassie stains are compatible with mass spectrometry. We strongly recommend that you mix your own coomassie stains (see protocol below for the recipe). We cannot recommend samples that have been stained with any commercial ‘instant’ or ‘ready-to-use’ staining kits.
We also do not accept gels that have been boiled or microwaved to speed up the staining or destaining process. Please never use overhead projector foils for gel scanning as this prevents the gel from being used for MS experiments.
If you have gels from which you like individual bands to be identified by MS, you have two options. First, you can send (or bring) the gel to the facility, and we cut out the band(s) that you indicate.
Alternatively, you can cut out gel bands yourself. In this second scenario, please take precautions to prevent contamination (keratins, etc).
The molecular mass of proteins is best determined under denaturing conditions. Please observe the following guidelines for sample preparation: