This facility provides a full proteomics infrastructure for the identification and characterisation of proteins.
The Proteomics Core Facility maintains and uses equipment for protein and peptide separation, as well as for sample preparation for mass spectrometry.
We are equipped with state-of-the-art chromatography systems and mass spectrometers for LC, MS and LC-MS/MS, with a wide range of bioinformatics tools for data interpretation and evaluation.
Tribrid linear ion trap-quadrupole-Orbitrap mass spectrometer. The tribrid architecture, with an independent ion-routing multipole which enables parallelization, significantly increases scan rates, and maximizes versatility. Any of three dissociation techniques – CID, HCD, and ETD – can be performed at any fragmentation stage, followed by analysis in either the linear ion trap or Orbitrap mass analyzer.
Hybrid quadrupole-Orbitrap mass spectrometer. Used for protein identification and quantification by LC-MS/MS. Principally used for complex samples and identification of post-translational modifications. HCD (high resolution) fragmentation allows for use of MS2 quantification strategies (eg. TMT labelling).
Quadrupole Time-of-Flight electrospray mass spectrometer. Used in combination with HPLC (C4 column) for mass determination of intact proteins
Nano HPLC System – used for peptide separation interfaced directly to the mass spectrometer for LC-MS/MS
Offline analytical HPLC systems with quarternary pumps, variable wavelength UV detector and micro fraction collector. For pre-fractionation of complex protein digests prior to LC-MS/MS. Operates in High pH reverse phase mode.
We perform our sample preparation in a semi-automated way using the KingFisher Apex (Thermo Fisher Scientific). The SP3 protocol and PTM enrichment (phosphorylation) are conducted with this system.