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Proteomics Core Facility

This facility provides a full proteomics infrastructure for the identification and characterisation of proteins.

Instrumentation and Software

The Proteomics Core Facility maintains and uses equipment for protein separation and imaging, as well as for sample preparation for mass spectrometry.

We are equipped with state-of-the-art chromatography systems and mass spectrometers for LC, MS and LC-MS/MS, with a wide range of bioinformatics tools for data interpretation and evaluation.

Mass Spectrometry

Orbitrap Fusion Lumos ETD

Orbitrap Fusion Lumos ETD

Tribrid linear ion trap-quadrupole-Orbitrap mass spectrometer. The tribrid architecture, with an independent ion-routing multipole which enables parallelization, significantly increases scan rates, and maximizes versatility. Any of three dissociation techniques – CID, HCD, and ETD – can be performed at any fragmentation stage, followed by analysis in either the linear ion trap or Orbitrap mass analyzer.

Q-Exactive Plus (Thermo Fisher)

Q-Exactive Plus (Thermo Fisher)

Hybrid quadrupole-Orbitrap mass spectrometer. Used for protein identification and quantification by LC-MS/MS. Principally used for complex samples and identification of post-translational modifications. HCD (high resolution) fragmentation allows for use of MS2 quantification strategies (eg. TMT labelling).

Q-Tof Premier (Waters)

Q-Tof Premier (Waters)

Quadrupole Time-of-Flight electrospray mass spectrometer. Used in combination with HPLC (C4 column) for mass determination of intact proteins


Liquid Chromatography

Ultimate 3000 RSLC System (Dionex)

Ultimate 3000 RSLC System (Dionex)

Nano LC System – used for peptide separation interfaced directly to the Q-Exactive Plus for LC-MS/MS


Offline Sample Fractionation

1200 Infinity HPLC (Agilent) / Ultimate 3000 HPLC (ThermoFisher Scientific)

1200 Infinity HPLC (Agilent) / Ultimate 3000 HPLC (ThermoFisher Scientific)

Offline analytical HPLC systems with quarternary pumps, variable wavelength UV detector and micro fraction collector. For pre-fractionation of complex protein digests prior to LC-MS/MS. Operates in High pH reverse phase mode.

SDS PAGE equipment

SDS PAGE equipment

Calibrated Densitometer (GS-900, Bio-Rad)


Software

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