This facility offers a collection of state-of-the-art light microscopy equipment and image processing tools.
This macro calculates the coefficient of variation (CV, ratio of standard deviation to mean) of the intensity in the confocal image acquired in standardized conditions. CV is a good indicator of the overall sensitivity of the confocal detector and can be used for characterizing and comparing detectors inside one and across different confocal systems and specific spectral ranges. The detector with highest sensitivity has lowest CV value. The input for macro is two consecutive images, detailed measurement protocol is below.
This macro is inspired by work of Zucker: R.M. Zucker and O.Price, Cytometry 44:273–294 (2001). Big thanks to Kota Miura for helpful suggestions regarding the macro programming.
Author:
Source:
download zip-file containing detector_CV_macro_260413.ijm, description_detector_CV.docx and sample image.
Installation:
Unzip the “detector_CV_macro_260413.ijm” file into your ImageJ Macro folder and run it in ImageJ
Requirements:
Further information:
If you have problems in installing / using this distribution just let us know. Please also don’t hesitate to report bugs.