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Advanced Light Microscopy Facility

This facility offers a collection of state-of-the-art light microscopy equipment and image processing tools.

Publications

QUAREP-LiMi: a community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy.

Nelson G, Boehm U, Bagley S, Bajcsy P, Bischof J, Brown CM, Dauphin A, Dobbie IM, Eriksson JE, Faklaris O, Fernandez-Rodriguez J, Ferrand A, Gelman L, Gheisari A, Hartmann H, Kukat C, Laude A, Mitkovski M, Munck S, North AJ, Rasse TM, Resch-Genger U, Schuetz LC, Seitz A, Strambio-De-Castillia C, Swedlow JR, Alexopoulos I, Aumayr K, Avilov S, Bakker GJ, Bammann RR, Bassi A, Beckert H, Beer S, Belyaev Y, Bierwagen J, Birngruber KA, Bosch M, Breitlow J, Cameron LA, Chalfoun J, Chambers JJ, Chen CL, Conde-Sousa E, Corbett AD, Cordelieres FP, Nery ED, Dietzel R, Eismann F, Fazeli E, Felscher A, Fried H, Gaudreault N, Goh WI, Guilbert T, Hadleigh R, Hemmerich P, Holst GA, Itano MS, Jaffe CB, Jambor HK, Jarvis SC, Keppler A, Kirchenbuechler D, Kirchner M, Kobayashi N, Krens G, Kunis S, Lacoste J, Marcello M, Martins GG, Metcalf DJ, Mitchell CA, Moore J, Mueller T, Nelson MS, Ogg S, Onami S, Palmer AL, Paul-Gilloteaux P, Pimentel JA, Plantard L, Podder S, Rexhepaj E, Royon A, Saari MA, Schapman D, Schoonderwoert V, Schroth-Diez B, Schwartz S, Shaw M, Spitaler M, Stoeckl MT, Sudar D, Teillon J, Terjung S, Thuenauer R, Wilms CD, Wright GD, Nitschke R

Journal of microscopy 2021

34214188. doi:10.1111/jmi.13041.

REMBI: recommended metadata for biological images-enabling reuse of microscopy data in biology.

Sarkans U, Chiu W, Collinson L, Darrow MC, Ellenberg J, Grunwald D, Hériché JK, Iudin A, Martins GG, Meehan T, Narayan K, Patwardhan A, Russell MRG, Saibil HR, Strambio-De-Castillia C, Swedlow JR, Tischer C, Uhlmann V, Verkade P, Barlow M, Bayraktar O, Birney E, Catavitello C, Cawthorne C, Wagner-Conrad S, Duke E, Paul-Gilloteaux P, Gustin E, Harkiolaki M, Kankaanpää P, Lemberger T, McEntyre J, Moore J, Nicholls AW, Onami S, Parkinson H, Parsons M, Romanchikova M, Sofroniew N, Swoger J, Utz N, Voortman LM, Wong F, Zhang P, Kleywegt GJ, Brazma A

Nature methods 2021

34021280. doi:10.1038/s41592-021-01166-8.

A high throughput SEC23 functional interaction screen reveals a role for focal adhesion and extracellular matrix signalling in the regulation of COPII subunit SEC23A

Jung J, Khan MM, Landry J, Halavatyi A, Machado P, Reiss M, Pepperkok R

2021

doi:10.1101/2021.03.16.435679.

Microscopy-based assay for semi-quantitative detection of SARS-CoV-2 specific antibodies in human sera: A semi-quantitative, high throughput, microscopy-based assay expands existing approaches to measure SARS-CoV-2 specific antibody levels in human sera.

Pape C, Remme R, Wolny A, Olberg S, Wolf S, Cerrone L, Cortese M, Klaus S, Lucic B, Ullrich S, Anders-Össwein M, Wolf S, Cerikan B, Neufeldt CJ, Ganter M, Schnitzler P, Merle U, Lusic M, Boulant S, Stanifer M, Bartenschlager R, Hamprecht FA, Kreshuk A, Tischer C, Kräusslich HG, Müller B, Laketa V

BioEssays : news and reviews in molecular, cellular and developmental biology 2020

33377226. doi:10.1002/bies.202000257.

Integrative imaging reveals SARS-CoV-2-induced reshaping of subcellular morphologies.

Cortese M, Lee JY, Cerikan B, Neufeldt CJ, Oorschot VMJ, Köhrer S, Hennies J, Schieber NL, Ronchi P, Mizzon G, Romero-Brey I, Santarella-Mellwig R, Schorb M, Boermel M, Mocaer K, Beckwith MS, Templin RM, Gross V, Pape C, Tischer C, Frankish J, Horvat NK, Laketa V, Stanifer M, Boulant S, Ruggieri A, Chatel-Chaix L, Schwab Y, Bartenschlager R

Cell host & microbe 2020

33245857. doi:10.1016/j.chom.2020.11.003.

A joint action in times of pandemic: the German BioImaging recommendations for operating imaging core facilities during the SARS-Cov-2 emergency.

Dietzel S, Ferrando-May E, Fried H, Kukat C, Naumann A, Nitschke R, Pasierbek P, Peychl J, Rasse TM, Schroth-Diez B, Stöckl MT, Terjung S, Thuenauer R, Tulok S, Weidtkamp-Peters S, German BioImaging-Society for Microscopy and Image Analysis

Cytometry. Part A : the journal of the International Society for Analytical Cytology 2020

32583531. doi:10.1002/cyto.a.24178.

One step ahead: Innovation in core facilities.

Lippens S, D'Enfert C, Farkas L, Kehres A, Korn B, Morales M, Pepperkok R, Premvardhan L, Schlapbach R, Tiran A, Meder D, Van Minnebruggen G

EMBO reports 2019

30872318. doi:10.15252/embr.201948017.

FRAP and other photoperturbation techniques.

Halavatyi A, Terjung S

2017

doi:10.1002/9781119096948.ch5.

Measuring molecular dynamics and interactions by Förster Resonance Energy Transfer (FRET).

Halavatyi A, Terjung S

2017

doi:10.1002/9781119096948.ch4.

Acetylated tubulin is essential for touch sensation in mice

Morley SJ, Qi YM, Iovino L, Andolfi L, Guo D, Kalebic N, Castaldi L, Tischer C, Portulano C, Bolasco G, Shirlekar K, Fusco CM, Asaro A, Fermani F, Sundukova M, Matti U, Reymond L, De Ninno A, Businaro L, Johnsson K, Lazzarino M, Ries J, Schwab Y, Hu J, Heppenstall PA

ELIFE 2016

27976998. doi:10.7554/eLife.20813.

Fluorescent Imaging Techniques: FRET and Complementary Methods.

Terjung S, Belyaev Y

2015

doi:10.1201/b18007-4.

Systematic analysis of human protein complexes identifies chromosome segregation proteins

Hutchins JR, Toyoda Y, Hegemann B, Poser I, Hériché JK, Sykora MM, Augsburg M, Hudecz O, Buschhorn BA, Bulkescher J, Conrad C, Comartin D, Schleiffer A, Sarov M, Pozniakovsky A, Slabicki MM, Schloissnig S, Steinmacher I, Leuschner M, Ssykor A, Lawo S, Pelletier L, Stark H, Nasmyth K, Ellenberg J, Durbin R, Buchholz F, Mechtler K, Hyman AA, Peters JM

Science (New York, N.Y.) 2010

20360068. doi:10.1126/science.1181348.

A genome wide RNAI screen by time lapse microscopy in order to identify mitotic genes - Computational aspects and challenges.

Walter T, Held M, Neumann B, Heriche JK, Conrad C, Pepperkok R, Ellenberg J

2008 IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING: FROM NANO TO MACRO, VOLS 1-4 2008

doi:10.1109/ISBI.2008.4540999.

EAMNET - a resource and teaching framework for advanced light microscopy

Terjung S, Pepperkok R, Ankerhold R, Caiolfa VR, Castel S, Majer P, Geiger B, Luini A, Salamero J, Shorte S, Small JV

European journal of cell biology 2005

doi:.

Immunofluorescence microscopy

Pepperkok R, Girod A, Simpson J, Rietdorf J

2000

doi:.


QUAREP-LiMi: a community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy.

Nelson G, Boehm U, Bagley S, Bajcsy P, Bischof J, Brown CM, Dauphin A, Dobbie IM, Eriksson JE, Faklaris O, Fernandez-Rodriguez J, Ferrand A, Gelman L, Gheisari A, Hartmann H, Kukat C, Laude A, Mitkovski M, Munck S, North AJ, Rasse TM, Resch-Genger U, Schuetz LC, Seitz A, Strambio-De-Castillia C, Swedlow JR, Alexopoulos I, Aumayr K, Avilov S, Bakker GJ, Bammann RR, Bassi A, Beckert H, Beer S, Belyaev Y, Bierwagen J, Birngruber KA, Bosch M, Breitlow J, Cameron LA, Chalfoun J, Chambers JJ, Chen CL, Conde-Sousa E, Corbett AD, Cordelieres FP, Nery ED, Dietzel R, Eismann F, Fazeli E, Felscher A, Fried H, Gaudreault N, Goh WI, Guilbert T, Hadleigh R, Hemmerich P, Holst GA, Itano MS, Jaffe CB, Jambor HK, Jarvis SC, Keppler A, Kirchenbuechler D, Kirchner M, Kobayashi N, Krens G, Kunis S, Lacoste J, Marcello M, Martins GG, Metcalf DJ, Mitchell CA, Moore J, Mueller T, Nelson MS, Ogg S, Onami S, Palmer AL, Paul-Gilloteaux P, Pimentel JA, Plantard L, Podder S, Rexhepaj E, Royon A, Saari MA, Schapman D, Schoonderwoert V, Schroth-Diez B, Schwartz S, Shaw M, Spitaler M, Stoeckl MT, Sudar D, Teillon J, Terjung S, Thuenauer R, Wilms CD, Wright GD, Nitschke R

Journal of microscopy 2021

34214188. doi:10.1111/jmi.13041.

REMBI: recommended metadata for biological images-enabling reuse of microscopy data in biology.

Sarkans U, Chiu W, Collinson L, Darrow MC, Ellenberg J, Grunwald D, Hériché JK, Iudin A, Martins GG, Meehan T, Narayan K, Patwardhan A, Russell MRG, Saibil HR, Strambio-De-Castillia C, Swedlow JR, Tischer C, Uhlmann V, Verkade P, Barlow M, Bayraktar O, Birney E, Catavitello C, Cawthorne C, Wagner-Conrad S, Duke E, Paul-Gilloteaux P, Gustin E, Harkiolaki M, Kankaanpää P, Lemberger T, McEntyre J, Moore J, Nicholls AW, Onami S, Parkinson H, Parsons M, Romanchikova M, Sofroniew N, Swoger J, Utz N, Voortman LM, Wong F, Zhang P, Kleywegt GJ, Brazma A

Nature methods 2021

34021280. doi:10.1038/s41592-021-01166-8.

A high throughput SEC23 functional interaction screen reveals a role for focal adhesion and extracellular matrix signalling in the regulation of COPII subunit SEC23A

Jung J, Khan MM, Landry J, Halavatyi A, Machado P, Reiss M, Pepperkok R

2021

doi:10.1101/2021.03.16.435679.

Microscopy-based assay for semi-quantitative detection of SARS-CoV-2 specific antibodies in human sera: A semi-quantitative, high throughput, microscopy-based assay expands existing approaches to measure SARS-CoV-2 specific antibody levels in human sera.

Pape C, Remme R, Wolny A, Olberg S, Wolf S, Cerrone L, Cortese M, Klaus S, Lucic B, Ullrich S, Anders-Össwein M, Wolf S, Cerikan B, Neufeldt CJ, Ganter M, Schnitzler P, Merle U, Lusic M, Boulant S, Stanifer M, Bartenschlager R, Hamprecht FA, Kreshuk A, Tischer C, Kräusslich HG, Müller B, Laketa V

BioEssays : news and reviews in molecular, cellular and developmental biology 2020

33377226. doi:10.1002/bies.202000257.

Integrative imaging reveals SARS-CoV-2-induced reshaping of subcellular morphologies.

Cortese M, Lee JY, Cerikan B, Neufeldt CJ, Oorschot VMJ, Köhrer S, Hennies J, Schieber NL, Ronchi P, Mizzon G, Romero-Brey I, Santarella-Mellwig R, Schorb M, Boermel M, Mocaer K, Beckwith MS, Templin RM, Gross V, Pape C, Tischer C, Frankish J, Horvat NK, Laketa V, Stanifer M, Boulant S, Ruggieri A, Chatel-Chaix L, Schwab Y, Bartenschlager R

Cell host & microbe 2020

33245857. doi:10.1016/j.chom.2020.11.003.

A joint action in times of pandemic: the German BioImaging recommendations for operating imaging core facilities during the SARS-Cov-2 emergency.

Dietzel S, Ferrando-May E, Fried H, Kukat C, Naumann A, Nitschke R, Pasierbek P, Peychl J, Rasse TM, Schroth-Diez B, Stöckl MT, Terjung S, Thuenauer R, Tulok S, Weidtkamp-Peters S, German BioImaging-Society for Microscopy and Image Analysis

Cytometry. Part A : the journal of the International Society for Analytical Cytology 2020

32583531. doi:10.1002/cyto.a.24178.

One step ahead: Innovation in core facilities.

Lippens S, D'Enfert C, Farkas L, Kehres A, Korn B, Morales M, Pepperkok R, Premvardhan L, Schlapbach R, Tiran A, Meder D, Van Minnebruggen G

EMBO reports 2019

30872318. doi:10.15252/embr.201948017.

FRAP and other photoperturbation techniques.

Halavatyi A, Terjung S

2017

doi:10.1002/9781119096948.ch5.

Measuring molecular dynamics and interactions by Förster Resonance Energy Transfer (FRET).

Halavatyi A, Terjung S

2017

doi:10.1002/9781119096948.ch4.

Acetylated tubulin is essential for touch sensation in mice

Morley SJ, Qi YM, Iovino L, Andolfi L, Guo D, Kalebic N, Castaldi L, Tischer C, Portulano C, Bolasco G, Shirlekar K, Fusco CM, Asaro A, Fermani F, Sundukova M, Matti U, Reymond L, De Ninno A, Businaro L, Johnsson K, Lazzarino M, Ries J, Schwab Y, Hu J, Heppenstall PA

ELIFE 2016

27976998. doi:10.7554/eLife.20813.

Fluorescent Imaging Techniques: FRET and Complementary Methods.

Terjung S, Belyaev Y

2015

doi:10.1201/b18007-4.

Systematic analysis of human protein complexes identifies chromosome segregation proteins

Hutchins JR, Toyoda Y, Hegemann B, Poser I, Hériché JK, Sykora MM, Augsburg M, Hudecz O, Buschhorn BA, Bulkescher J, Conrad C, Comartin D, Schleiffer A, Sarov M, Pozniakovsky A, Slabicki MM, Schloissnig S, Steinmacher I, Leuschner M, Ssykor A, Lawo S, Pelletier L, Stark H, Nasmyth K, Ellenberg J, Durbin R, Buchholz F, Mechtler K, Hyman AA, Peters JM

Science (New York, N.Y.) 2010

20360068. doi:10.1126/science.1181348.

A genome wide RNAI screen by time lapse microscopy in order to identify mitotic genes – Computational aspects and challenges.

Walter T, Held M, Neumann B, Heriche JK, Conrad C, Pepperkok R, Ellenberg J

2008 IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING: FROM NANO TO MACRO, VOLS 1-4 2008

doi:10.1109/ISBI.2008.4540999.

EAMNET – a resource and teaching framework for advanced light microscopy

Terjung S, Pepperkok R, Ankerhold R, Caiolfa VR, Castel S, Majer P, Geiger B, Luini A, Salamero J, Shorte S, Small JV

European journal of cell biology 2005

doi:.

Immunofluorescence microscopy

Pepperkok R, Girod A, Simpson J, Rietdorf J

2000

doi:.

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