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Advanced fluorescence imaging techniques – Course and Conference Office

EMBL Course

Advanced fluorescence imaging techniques

Overview

EMBL is committed to sharing research advances and sustaining scientific interaction throughout the coronavirus pandemic. We are delighted to announce that the course is going virtual and invite you to join us online.

Course Overview

This practical course will cover advanced light microscopy techniques and participants will learn how to derive qualitative and quantitative insights on molecular mechanisms in cells and developing organisms. Invited guest researchers together with experts from the EMBL and microscopy professionals will foster an intense information flow with a balance of lectures and practical workshops. The focus of the course will be on the use of fluorescence microscopy to obtain information about protein localisation, dynamics, and functions at different spatial scales.

Audience

This course is directed towards researchers in the life sciences with access to light microscopy equipment, who have an immediate need to apply the techniques they will learn to solve biological problems at the cellular and molecular level in vivo.

Modules/Resources

The topics will include fluorescence microscopy techniques such as fast time-lapse microscopy, spinning disk and point-scanning confocal microscopy, light sheet microscopy of cleared samples, photo-manipulation as a tool, multicolor single molecule localization microscopy (SMLM) with spectral demixing. For the demanding applications we will cover the aspects of image analysis and possibilities of microscope automation. The modules will cover all necessary knowledge and demonstrate tools to conduct similar pipelines at your home lab enabling you to answer emerging biological questions.

Course Format Information

The entire course, including hands-on practicals, will happen virtually. 

All participants will attend lectures and live Q&A sessions together each day between 13:00 and 16:00 (times might vary slightly depending on the day).

Some lectures will happen live, other lectures and tutorials will be prerecorded and provided to participants in advance. Each prerecorded module will be associated with live Q&A sessions to discuss with the speaker. For practicals, participants will be subdivided into four smaller groups. Two groups will attend the practicals each day between 16:00 and 19:00, two other groups will attend the practicals from 9:00 to 12:00.

Online practical sessions will run on four high-end microscopes located at four different sites. Practical providers will set up the audio-visual equipment, including cameras, to explain and discuss the hardware with participants. Then the screens of microscope computers will be shared with participants via Zoom. In some practicals participants will get an opportunity to control the software remotely to configure new experiments and adjust experiment settings.

Learning Outcomes

Participants will get an overview about advanced fluorescence imaging techniques.

This course is kindly supported by the EMBL Advanced Light Microscopy Facility.

This course is organised in cooperation with Olympus.

Date: 16 - 20 May 2022

Location: Virtual


Deadline(s):

Application: Closed


Organisers:

  • Joana Delgado
    University of Zurich, Switzerland
    • Manuel Gunkel
      EMBL Heidelberg, Germany
      • Aliaksandr Halavatyi
        EMBL Heidelberg, Germany

EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme

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