
Ed Boyden
Massachusetts Institute of Technology
USA
(Virtual Speaker)
EMBL Course
Please see EMBL’s COVID-19 safety policy for testing and other requirements to attend this on-site course.
Expansion microscopy (ExM) physically magnifies specimens, allowing to obtain super-resolution images using a conventional diffraction-limited microscope such as confocal microscopy. This powerful technique is easy to implement but it requires some hands-on training. This workshop aims at disseminating and discussing expansion microscopy protocols and is intended for cell biologists, parasitologists, etc, with applications ranging from tissue to cells.
This course is intended for researchers ranging from PhD students to post-docs and senior scientists as well as staff from bioimaging platforms who wish to establish expansion microscopy in their respective institutes.
After this course you should be able to:
Massachusetts Institute of Technology
USA
(Virtual Speaker)
University of Geneva
Switzerland
EMBL Heidelberg
Germany
Swiss Federal Institute of Technology Lausanne
Switzerland
University of Illinois Chicago
USA
(Virtual Speaker)
University of Geneva
Switzerland
University of Geneva
Switzerland
University of Geneva
Switzerland
University of Sheffield
UK
Utrecht University
The Netherlands
University of Geneva
Switzerland
EMBL Heidelberg
Germany
EMBL Heidelberg
Germany
Julius Maximilian University of Würzburg
Germany
EMBL Heidelberg
Germany
EMBL Heidelberg
Germany
University of Washington
USA
(Virtual Speaker)
EMBL Heidelberg
Germany
EMBL Heidelberg
Germany
University of Geneva
Switzerland
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Time | Speaker | Location |
---|---|---|
08:40 | Bus departure from Premier Inn Heidelberg City Centre Hotel to EMBL Heidelberg | Premier Inn Heidelberg City Centre Hotel bus stop |
09:00-09:30 | EMBL intro & House notes | Lecture Hall 103 |
09:30-09:45 | Course overview | Lecture Hall 103 |
09:45-10:45 | Keynote lecture | Lecture Hall 103 |
10:45-11:15 | Coffee break | Lecture Hall 103 |
11:15-12:45 | Icebreaking activity | Lecture Hall 103 |
12:45-13:30 | Lunch break | Canteen |
13:30-15:00 | Workstations by groups | TBA |
13:30-14:15 | Workstation 1: Cryo-fixation Gp1 | TBA |
Workstation 2: HPF Gp2 | TBA | |
Practical lectures: Expansion protocols: Gp3/Gp4 | TBA | |
14:15-15:30 | Workstation 1: Cryo-fixation Gp2 | TBA |
Workstation 2: HPF Gp1 | TBA | |
Practical lectures: Expansion protocols: Gp3/Gp4 | TBA | |
15:30-16:30 | Lecture | Lecture Hall 103 |
16:30-17:00 | Coffee break | Lecture Hall 103 |
17:00-18:30 | Workstations by groups | TBA |
17:00-17:45 | Workstation 1: Cryo-fixation Gp3 | TBA |
Workstation 2: HPF Gp4 | TBA | |
Practical lectures: Expansion protocols: Gp1/Gp2 | TBA | |
17:45-18:30 | Workstation 1: Cryo-fixation Gp4 | TBA |
Workstation 2: HPF Gp3 | TBA | |
Practical lectures: Expansion protocols: Gp1/Gp2 | TBA | |
18:30-19:30 | Pre-dinner drinks | TBA |
19:30-20:30 | Dinner | Canteen |
20:45 | Bus departure to Premier Inn Heidelberg City Centre Hotel | EMBL bus stop |
Time | Speaker | Location |
---|---|---|
08:30 | Bus departure from Premier Inn Heidelberg City Centre Hotel to EMBL Heidelberg | Premier Inn Heidelberg City Centre Hotel bus stop |
08:45-09:00 | Overview of the day | Lecture Hall 103 |
09:00-09:15 | Workstation – Freeze substitution Gp1/Gp2/Gp3/Gp4 | TBA |
09:15-10:00 | Lecture | Lecture Hall 103 |
10:00-10:45 | Workstation – Sample rehydration Gp1/Gp2/Gp3/Gp4 | TBA |
Workstation – U-ExM and iU-ExM STEP 1 – Gp1/Gp2/Gp3/Gp4 | ||
10:45-11:30 | Lecture | Lecture Hall 103 |
11:30-12:00 | Coffee break | Lecture Hall 103 |
12:00-12:45 | Lecture | Lecture Hall 103 |
12:45-13:45 | Lunch break | Canteen |
13:45-14:15 | Workstation – U-ExM and iU-ExM STEP 2 – Gp1/Gp2/Gp3/Gp4 | TBA |
14:15-15:00 | Coffee break | Lecture Hall 103 |
15:00-15:30 | Workstation – U-ExM and iU-ExM STEP 3 – Gp1/Gp2/Gp3/Gp4 | TBA |
15:45-16:30 | Lecture | Lecture Hall 103 |
17:00-17:30 | Workstation – U-ExM and iU-ExM STEP 4 – Gp1/Gp2/Gp3/Gp4 | TBA |
17:30-18:15 | Lecture | Lecture Hall 103 |
18:15-20:00 | Tips & Tricks with Pizza Dinner | Lecture Hall 103 |
20:15 | Bus departure to Premier Inn Heidelberg City Centre Hotel | EMBL bus stop |
Time | Speaker | Location |
---|---|---|
08:40 | Bus departure from Premier Inn Heidelberg City Centre Hotel to EMBL Heidelberg | Premier Inn Heidelberg City Centre Hotel bus stop |
09:00-09:15 | Overview of the day | Lecture Hall 103 |
09:15-10:00 | Workstation – Immunostaining STEP 1 – Gp1/Gp2/Gp3/Gp4 | TBA |
10:00-10:45 | Lecture | Lecture Hall 103 |
10:45-11:15 | Coffee break | Lecture Hall 103 |
11:15-12:00 | Lecture | Lecture Hall 103 |
12:00-13:00 | Lunch break | Canteen |
13:00-13:45 | Workstation – Immunostaining STEP 2 – Gp1/Gp2/Gp3/Gp4 | TBA |
13:45-14:15 | Coffee break | Lecture Hall 103 |
14:15-15:45 | Image analysis tutorial | TBA |
15:45-16:45 | Workstation – Immunostaining STEP 3 – Gp1/Gp2/Gp3/Gp4 | TBA |
17:00 | Bus departure to downtown | EMBL bus stop |
17:30-19:00 | Heidelberg Old Town tour | Neckarmünzplatz |
19:30 | Downtown dinner | TBA |
Time | Speaker | Location |
---|---|---|
08:00 | Bus departure from Premier Inn Heidelberg City Centre Hotel to EMBL Heidelberg | Premier Inn Heidelberg City Centre Hotel bus stop |
08:15-08:30 | Overview of the day | Lecture Hall 103 |
08:30-09:45 | Workstation – iU-ExM STEP 5 – Gp1 / Gp2 / Gp3 / Gp4 | TBA |
09:45-10:30 | Coffee break | Lecture Hall 103 |
10:30-11:00 | Workstation – iU-ExM STEP 6 – Gp1 / Gp2 / Gp3 / Gp4 | TBA |
11:00-12:45 | Imaging session (U-ExM) – Gp1 / Gp2 / Gp3 / Gp4 | TBA |
12:45-13:30 | Lunch break | Canteen |
13.30-14:15 | Workstation – iU-ExM STEP 7 – Gp1 / Gp2 / Gp3 / Gp4 | TBA |
14:15-15:15 | Imaging session (U-ExM) – Gp1 / Gp2 & Coffee break – Gp3 / Gp4 | TBA |
15:15-15:30 | Workstation – iU-ExM STEP 8 – Gp1 / Gp2 / Gp3 / Gp4 | TBA |
15:30-16:30 | Imaging session (U-ExM) – Gp3 / Gp4 & Coffee break – Gp1 / Gp2 | TBA |
16:30-17:00 | Workstation – iU-ExM STEP 8 – Gp1 / Gp2 / Gp3 / Gp4 | TBA |
17:00-17:30 | Workstation – iU-ExM STEP 9 – Gp1 / Gp2 / Gp3 / Gp4 | TBA |
17:30-18:30 | Imaging session (U-ExM) – Gp1 / Gp2 / Gp3 / Gp4 | TBA |
18:30-19:30 | Workstation – iU-ExM STEP 9 – Gp1 / Gp2 / Gp3 / Gp4 | TBA |
19.30-20:30 | Dinner | Canteen |
20:45 | Bus departure to Premier Inn Heidelberg City Centre Hotel | EMBL bus stop |
Time | Speaker | Location |
---|---|---|
08:30 | Bus departure from Premier Inn Heidelberg City Centre Hotel to EMBL Heidelberg | Premier Inn Heidelberg City Centre Hotel bus stop |
08.45-09.00 | Overview of the day | Lecture Hall 103 |
09.00-11.00 | Imaging session (iU-ExM) – Gp1 / Gp2 / Gp3 / Gp4 | TBA |
11.00-11.30 | Coffee break | Lecture Hall 103 |
11.30-13.00 | Imaging session (iU-ExM) – Gp1 / Gp2 / Gp3 / Gp4 | TBA |
13.00-14.00 | Lunch break | Canteen |
14.00-15.30 | Final discussion round | Lecture Hall 103 |
15.30-16.00 | Coffee break | Lecture Hall 103 |
16.00-16.30 | Feedback round & closing remarks | Lecture Hall 103 |
16:45 | Bus departure to Heidelberg train station and city center | EMBL bus stop |
The course is limited to 16 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.
Registration fees include admission, course materials, COVID-19 safety measures, meals and coffee breaks. Participants are expected to book and pay their own accommodation and travel expenses.
Academia | €675 |
PhD Student | €675 |
Industry | €1200 |
NO visa support letters will be issued until payment of the registration fee is confirmed.
The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.
After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.
Instructions
Please note:
For detailed instructions, please watch our video on how to submit a course motivation letter.
For further information about registration and motivation letter submission please refer to the FAQ page.
Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of both registration fee waivers and travel grants. Availability is limited to participants attending on-site EMBL Courses and virtual or on-site EMBO Practical Courses in Heidelberg, and will be indicated during the motivation letter submission process.
Your place in the course is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.
The fee waiver will cover the registration sum that you have paid to attend the course.
The travel grant will cover the cost of travel (airfare, train, bus, taxi, accommodation, visa, and/or registration fees*) and is provided up to specified caps which are normally as follows:
– up to €400 for participants travelling to an EMBL Course within Europe.
– up to €1000 for participants travelling to an EMBL Course from outside Europe.
– up to €500 for any participant travelling to an EMBO Practical Course.
– up to €1000 for any participant working in Chile, India, Singapore or Taiwan travelling to an EMBO Practical Course.
– up to €700 for any participant working in Croatia, Czech Republic, Estonia, Greece, Hungary, Italy, Lithuania, Luxembourg, Poland, Slovenia, and Turkey travelling to an EMBO Practical Course.
*Registration fees are only covered for EMBO Practical Courses
The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.
Applies to selected courses only. Availability will be indicated during the motivation letter submission process.
This grant covers costs related to your attendance at the course (registration, travel and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.
Whether you are eligible to apply for a travel grant, depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):
– for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course
– for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course
You may apply for financial assistance when submitting your motivation letter for courses. In your application, you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.
For the Boehringer Ingelheim Fonds Travel Grant, there is a pre-application question during the motivation letter submission process, and if selected you will be requested to complete a standard form and documentation consisting of your travel expense estimation.
The scientific organisers will select the recipients of all financial assistance during the motivation letter selection process. Results will be announced approximately 6-8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the course. Selection is based on scientific merit, your current work or study location, the reasons for needing financial support, and the impact this event will have on your career.
Costs will be reimbursed after the meeting only once a reimbursement form and original receipts (from travel costs) have been received.
See our list of external funding opportunities and for further information about financial assistance please refer to the FAQ page.
Single rooms have been reserved for 5 nights (21-26 May) at the Premier Inn Heidelberg City Centre Hotel. After the selection process, selected participants should contact the hotel directly to make a booking (booking code EIC23-02). We recommend that all course participants stay at the Premier Inn City Center Hotel which is the dedicated hotel for the course.
Shuttle buses will go from the Premier Inn Heidelberg City Centre Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.
Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany
For further information on getting to EMBL Heidelberg visit our Travel Information page.
For enquiries about accommodation and local transportation please refer to the FAQ page.
The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus.
Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.
COVID-19 information for on-site events at EMBL Heidelberg can be found in our COVID-19 FAQs.
Event supporters:
Media partners
FocalPlane, a The Company of Biologists microscopy community site
Sponsorship opportunities
We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other events, or contact sponsorship@embl.de for further information.
If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.
EMBL wishes to warn sponsors of EMBL conferences and courses of fraudulent schemes purporting to offer sponsorship opportunities on behalf of EMBL or affiliated with EMBL officials. One current scam campaign of which we are aware is conducted using the name ‘Judy Eastman’ (judy@gopcontact.a2hosted.com) and entails approaches to sponsors offering sponsorship opportunities on EMBL’s behalf. Please be kindly advised that all relevant communication regarding sponsorship of EMBL conferences, symposia and courses is handled by EMBL directly and is sent from an official EMBL account. EMBL does not work with any external providers on sponsorship acquisition.
Please also note that:
Suspicious communications purportedly from, for or on behalf of EMBL should be reported to EMBL at the following email address sponsorship@embl.de.
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Date: 22 - 26 May 2023
Location: EMBL Heidelberg
Venue: EMBL Imaging Centre
Deadline(s):
Application: Closed
Organisers:
Contact: Events
EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme