Time-resolved STED nanoscopy in life sciences

Overview

Course overview

This six-day comprehensive advanced microscopy course focuses on stimulated emission depletion microscopy (STED) and its application to biological questions. Through lectures, tutorials and practical workshops, the course provides in-depth knowledge on all aspects of STED Nanoscopy. It covers the fundamentals of STED microscopy, different technical implementations and has a strong emphasis on sample preparation – including choice of fluorophores and refractive index matching, as well as image acquisition strategies for fixed samples and living cells. Students will have the opportunity for exercises on state-of the art STED microscopes in which they will acquire high quality nanoscopy data. The faculty consists of invited guest researchers, experts from the EMBL and Leica Microsystems.

Audience

This course is designed for researchers at the early stages of their career in the life sciences (e.g. pre or postdoctoral level), ideally with access to a STED microscope. The participants should have an immediate need to apply the trained skills for their biological questions. The course is strongly centered on practical aspects of STED microscopy and is therefore especially suited for people who rely on STED nanoscopy for their research.

Modules/Resources

The course will cover the entire range of skills needed to apply STED nanoscopy in life sciences. The following topics will be covered in lectures, tutorials as well as in practical workshops:

• Sample preparation for STED microscopy, including choice of fluorophores and refractive index matching
• Technical implementations – pulsed-STED, gated-STED and time-resolved STED (tauSTED)
• Multi-colour 2D and 3D STED microscopy
• Image acquisition strategies for live-cell STED
• STED multiplexing via lifetime-based fluorophore separation
• Clearing for large volume STED imaging
• Expansion-microscopy STED
• Deconvolution of time-resolved STED data
• Image analysis

Learning Outcomes

At the end of the course, the participants will be able to design experiments using STED microscopy, prepare high-quality microscopy samples and acquire state-of-the-art nanoscopy images.

This course is organised in cooperation with Leica Microsystems.

What past participants say about the course

“The EMBL super-resolution microscopy course was exactly tailored to my needs and will allow me to get my own experiments running independently. Also, contacts I made will be very useful in the future!” – Paul Markus Müller, Freie Universität Berlin, Germany

“Very well organized and conceived course. Everything is covered here including the
fundamentals, the practical sessions, creative application, innovative development of STED technology. If you are interested in the nanoscopy, I would recommend you to participate in this course.” – Shao-Chun ‘Peggy’ Hsu, National Taiwan University, Taiwan

Sponsors

Media partners

Sponsorship opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other events, or contact sponsorship@embl.de for further information.

If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.

Media kit

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