EMBL Hamburg has established a fully automated platform for the high-throughput crystallization of biological macromolecules in order to address a common bottleneck in x-ray crystallography. The HTP platform offers a wide range of crystallization screening conditions and flexible methods have been developed to adapt each step to individual project requirements. The services are available to the general user community.
Access the SPC crystallisation platform (CRIMS) here.
The identification of crystallisation conditions for biological molecules largely relies on a trial-and-error process in which a number of parameters are explored in large screening experiments. A significant effort is dedicated to sample characterization and quality-control experiments in order to identify at an early stage and prioritize those samples which would be more likely to crystallize.
The SPC facility can perform MALDI-TOF analysis, thermal denaturation assay, nanoDSF or DLS on samples submitted to the HTP crystallization platform on request. These methods provide additional information to the users which can be used to optimize protein stability and determine optimal temperatures for crystallization experiments.