Sample Preparation and Characterisation Facility


The following publications are (partly) based on experiments performed at the SPC:


Current Research in Structural Biology 3. 2021 Mar 5; 85-94. DOI:10.1016/j.crstbi.2021.03.002 Cyclohexyl-α maltoside as a highly efficient tool for membrane protein studies. Missel J.W, Salutros N, Ramos Becares E, Steffen J H, Gerdt Laursen A, Struve Garcia A, Garcia-Alai M, Kolar C, Gourdon P, Gotfryd K.


Nat Commun. 2020 Mar 11;11(1):1314. DOI: 10.1038/s41467-020-14862-4.
Structures of peptide-free and partially loaded MHC class I molecules reveal mechanisms of peptide selection.
Anjanappa R, Garcia-Alai M, Kopicki JD, Lockhauserbäumer J, Aboelmagd M, Hinrichs J, Nemtanu IM, Uetrecht C, Zacharias M, Springer S, Meijers R.


Sci Immunol. 2019 Jul 19;4(37). pii: eaav0860. DOI: 10.1126/sciimmunol.aav0860.
High-throughput peptide-MHC complex generation and kinetic screenings of TCRs with peptide-receptive HLA-A*02:01 molecules.
Moritz A, Anjanappa R, Wagner C, Bunk S, Hofmann M, Pszolla G, Saikia A, Garcia-Alai M, Meijers R, Rammensee HG, Springer S, Maurer D.

Sci Immunol. 2019 Jul 19;4(37). pii: eaau9039. DOI: 10.1126/sciimmunol.aau9039.
Empty peptide-receptive MHC class I molecules for efficient detection of antigen-specific T cells.
Saini SK, Tamhane T, Anjanappa R, Saikia A, Ramskov S, Donia M, Svane IM, Jakobsen SN, Garcia-Alai M, Zacharias M, Meijers R, Springer S, Hadrup SR.

Sci Rep. 2019 Jul 17;9(1):10379. DOI: 10.1038/s41598-019-46686-8.
High-throughput stability screening for detergent-solubilized membrane proteins.
Kotov V, Bartels K, Veith K, Josts I, Subhramanyam UKT, Günther C, Labahn J, Marlovits TC, Moraes I, Tidow H, Löw C, Garcia-Alai MM.

Biochim Biophys Acta Mol Cell Res. 2019 Jul;1866(7):1162-1170. DOI: 10.1016/j.bbamcr.2018.12.010
Novel CaM-binding motif in its NudT9H domain contributes to temperature sensitivity of TRPM2.
Gattkowski E, Johnsen A, Bauche A, Möckl F, Kulow F, Garcia Alai M, Rutherford TJ, Fliegert R, Tidow H.


Acta Crystallogr F Struct Biol Commun. 2018 Jan 1;74(Pt 1):23-30. DOI:10.1107/S2053230X17017381. Epub 2017 Dec 15.
Structure and stability of the Human respiratory syncytial virus M2-1 RNA-binding core domain reveals a compact and cooperative folding unit.
Molina IG, Josts I, Almeida Hernandez Y, Esperante S, Salgueiro M, Garcia Alai MM, de Prat-Gay G, Tidow H.

Nat Commun. 2018 Jan 23;9(1):328. DOI:10.1038/s41467-017-02443-x
Epsin and Sla2 form assemblies through phospholipid interfaces.
Garcia-Alai MM, Heidemann J, Skruzny M, Gieras A, Mertens HDT, Svergun DI, Kaksonen M, Uetrecht C, Meijers R.

Structure. 2018 Feb 6;26(2):345-355.e5. doi:  10.1016/j.str.2018.01.007
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research.
Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C.

Structure. 2018 Mar 6;26(3):467-476.e4. doi: 10.1016/j.str.2018.01.005. Epub 2018 Feb 8.
Multispecific Substrate Recognition in a Proton-Dependent Oligopeptide Transporter.
Martinez Molledo M, Quistgaard EM, Flayhan A, Pieprzyk J, Löw C.

J Synchrotron Radiat. 2018 Mar 1;25(Pt 2):361-372. DOI:10.1107/S1600577517017568. Epub 2018 Feb 13.
Multi-channel in situ dynamic light scattering instrumentation enhancing biological small-angle X-ray scattering experiments at the PETRA III beamline P12.
Falke S, Dierks K, Blanchet C, Graewert M, Cipriani F, Meijers R, Svergun D, Betzel C.

Neuron. 2018 Mar 21;97(6):1261-1267.e4. DOI:10.1016/j.neuron.2018.02.010. Epub 2018 Mar 1.
Structural Basis for Draxin-Modulated Axon Guidance and Fasciculation by Netrin-1 through DCC.
Liu Y, Bhowmick T, Liu Y, Gao X, Mertens HDT, Svergun DI, Xiao J, Zhang Y, Wang JH, Meijers R.


Sci Rep. 2017 Mar 9;7:44297. DOI:10.1038/srep44297
High-efficient production and biophysical characterisation of nicastrin and its interaction with APPC100.
Yu K, Yang G, Labahn J.

PLoS One. 2017 Mar 6;12(3):e0173126. DOI:10.1371/journal.pone.0173126. eCollection 2017.
Structure determination of a major facilitator peptide transporter: Inward facing PepTSt from Streptococcus thermophilus crystallized in space group P3121.
Quistgaard EM, Martinez Molledo M, Löw C.

J Synchrotron Radiat. 2017 Jan 1;24(Pt 1):323-332. DOI:10.1107/S1600577516016465. Epub 2017 Jan 1.
P13, the EMBL macromolecular crystallography beamline at the low-emittance PETRA III ring for high- and low-energy phasing with variable beam focusing.
Cianci M, Bourenkov G, Pompidor G, Karpics I, Kallio J, Bento I, Roessle M, Cipriani F, Fiedler S, Schneider TR.

Structural and functional basis of mitochondrial tRNA processing
Author: Sridhara, Sagar Date: 2017-12-20

Chembiochem. 2017 Sep 5;18(17):1735-1742. DOI:10.1002/cbic.201700235. Epub 2017 Jul 17.
Lipid-like Peptides can Stabilize Integral Membrane Proteins for Biophysical and Structural Studies.
Veith K, Martinez Molledo M, Almeida Hernandez Y, Josts I, Nitsche J, Löw C, Tidow H.