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Metabolomics Core Facility

This facility provides services for analysis of metabolites and lipids.

The facility is equipped with a high-resolution mass spectrometry system (HRMS) coupled with liquid chromatography (UHPLC) and direct infusion (NanoMate) for separation and detection of various classes of small molecules, metabolites and lipids.
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Michael Zimmermann

Group Leader

ORCID: 0000-0002-5797-3589

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Major projects and accomplishments

Identification and detection of various classes of small molecules, metabolites, and lipids in various biological matrices
EMBL-MCF mass spectral library from over 700 metabolites & EMBL-MCF 2.0 (LC-MS/MS library using low-adsorption chromatopgraphy)
Lipidomics profiling platform for comprehensive analysis of lipid species from multiple lipid classes (e.g. glycerols (TGs, DGs), phospholipids, ceramides, sphingomyelins, free fatty acids, cholesterylesters, etc.)
Absolute quantitation of metabolites and lipids using internal standards
Analysis of drugs and related metabolites

Services provided

We provide services for mass spectrometry-based analysis of small molecules, metabolites and lipids.

We perform targeted and untargeted measurements of various classes of molecules ranging from small, polar metabolites (amino acids, nucleotides, sugars, phosphates) to lipids in a variety of biological matrices.

Further services

Global untargeted metabolomics and lipidomics profiling
Targeted metabolomics profiling
Free fatty acid analysis
Targeted PFAS analysis
Metabolite and lipid identification using MS and MS/MS analysis
Drug and drug metabolite analysis
Consultation of experimental study design
Data processing incl. metabolite & lipid identification (level 1 confidence), visualization, and statistical analysis
Data archiving and sharing/publishing

Acknowledgment and Authorship

As per EMBL Internal Publication Policy (No. 63); if the facility services were used in work described in a publication, the facility must be acknowledged. For acknowledgment, the following statement must be added:

We acknowledge the support of the EMBL Metabolomics Core Facility (MCF) in the acquisition and analysis of liquid chromatography-mass spectrometry data.

In addition, if a staff member of the Metabolomics Core Facility contributes substantially to a published work, the staff member should be included as an author in the published work.

More information on this topic can be found here: Recommended guidelines & Acknowledging and citing core facilities