The ALMF offers support for microscope automation – Adaptive Feedback Microscopy
Adaptive Feedback Microscopy is a powerful methodology for performing high-content microscopy experiments in an automated manner. Initially acquired images are automatically analyzed and coordinates of the features of interest are communicated back to trigger follow-up acquisitions.
In particular, we offer:
- Establishing automated acquisition protocols, including automated scanning of multi-well plates.
- Microscope experiments with online image analysis, where objects of interest are detected automatically and a complex acquisition protocol is launched for these objects.
Example: Fuqua et al. Nature (2020)
- To this end we have developed software interfaces between confocal microscopes (Leica SP5, Zeiss Zen Black and Zen Blue, Olympus FV3000) that establish a feedback loop between popular image analysis packages (Cellprofiler, Fiji) and the microscope software.
- Experiments involving liquid handling on the microscope stage (e.g. adding a drug) using automated dispensing systems.