Open access to cutting-edge electron and light microscopy
We provide researchers from Europe and beyond with a synergistic portfolio of imaging services including cryo-EM, super-resolution and intravital microscopy to enable new ground-breaking research that crosses the scales of biology.
In our lab we work on the structural analysis of different amyloid fibrils, including fibrils from Alzheimer’s disease. But at the moment our focus is mainly on ex vivo fibrils from systemic amyloidosis (AA-amyloidosis and AL-amyloidosis). I’m working with different amyloid fibrils and I try to reconstruct them at high resolution to create an atomic model.
At our university we have two EMs made by JEOL, which we use for our work, a JEM-1400Plus (120 kV EM used mostly for negative stain purposes) and a JEM-2100F (200 kV EM used for scanning cryo grids). With our setup it is not possible to collect large amounts of high-quality data for reconstructions around 3Å resolution.
I also had previous experience with EMBL thanks to a collaboration, and Heidelberg is close by, so EMBL seemed like a good choice.
The samples I’m working with are often unevenly distributed on a grid and in the holes. Wim Hagen helped find a solution to manually pick the sample in an efficient way with SerialEM. This allowed us to obtain a dataset with images of the sample and not a majority (80%) of empty ones. If the sample is good, I get the results I need.
Some of the results were published recently:
The flexibility to solve problems and the dedication to getting the best and most data are great.
Yes, go! You will get high-quality data. I don’t know a better place for that.
Yes, I’m planning to.