Cutting-edge electron and light microscopy technologies
The user stories listed so far are based on the EMBL Cryo-EM Service Platform.
Weizmann Institute of Science, Israel
“If there’s a possibility to get scope time in Heidelberg, that would be a better choice than any other microscope in the world today, just because of the little tweaks they made. We were able for the first time to determine the structure of FAS-1 multi-enzyme complex to high enough resolution to now try the approach to locate the drug bound to the complex.” Read more
Institute of Biochemistry, ETH Zurich, Switzerland
“Using the data collected at EMBL, we were able to reconstruct the 3D structure of the membrane adenylyl cyclase AC9 bound to a G protein alpha subunit at 3.4 Å resolution and publish it in Science in April 2019.” Read more
Department of Biological Sciences, Birkbeck College, United Kingdom
“At EMBL we collected a dataset that we used to obtain a 4.9 Å structure of the assembled COPII coat, now published in Nature Communications. This is the highest-resolution structure of a membrane-assembled coat, and one of the highest-resolution subtomogram averages obtained so far.” Read more
Institute of Genetics and Molecular and Cellular Biology, France
“The resolution of data we collected at EMBL was high enough to visualise the bound substrate in the active site, and a single dataset allowed us to directly visualise two mutually exclusive states – the reactivated, substrate-bound state of RNA polymerase and the Gre factor-bound state.” Read more
Institute of Protein Biochemistry, Ulm University, Germany
“The samples I’m working with are often unevenly distributed on a grid and in the holes. Wim Hagen from EMBL Cryo-EM platform helped find a solution to manually pick the sample in an efficient way with SerialEM and get good results for two Nature Communications papers.” Read more