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EMBL Imaging Centre

Open access to cutting-edge electron and light microscopy

We provide researchers from Europe and beyond with a synergistic portfolio of imaging services including cryo-EM, super-resolution and intravital microscopy to enable new ground-breaking research that crosses the scales of biology.

Cryo-FIB Milling for Lamella Preparation

Aquilos 2

The Aquilos 2 (focused ion beam – scanning electron microscope) from Thermo Fisher Scientific enables the preparation of cellular lamellae from vitreous samples suitable for high-resolution cryo-electron tomography analyses. Plunge frozen as well as high-pressure frozen samples can be processed at cryo temperature by standard FIB milling and cryo-lift out approaches, respectively. Automated FIB milling routines based on commercial and in-house developed software packages enable unsupervised overnight operations for high-throughput milling.

Unique features

  • Automated cryo lamella preparation
  • Nanomanipulator for cryo-lift out
  • Integrated widefield fluorescence microscopy

Important specifications

  • Serial-FIB and AutoTEM software for automated lamella preparation
  • EasyLift NanoManipulator
  • Integrated Fluorescence Light Microscope (iFLM)
Lamella through plunge frozen yeast cells imaged with the iFLM inside the Aquilos 2. Credit Zhengyi Yang / EMBL.
Lamella through plunge frozen yeast cells imaged with the iFLM inside the Aquilos 2.
Thermo Fisher Scientific Aquilos 2. Credit: Stuart Ingham/EMBL.
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