Edit
Join the Basics of cryo-FIB milling and in situ cryo-electron tomography course (13–17 October 2025) – EMBL Imaging Centre

EMBL Imaging Centre

Open access to cutting-edge electron and light microscopy

We provide researchers from Europe and beyond with a synergistic portfolio of imaging services including cryo-EM, super-resolution and intravital microscopy to enable new ground-breaking research that crosses the scales of biology.

virginia.pierini@embl.de

Category: General

Join the Basics of cryo-FIB milling and in situ cryo-electron tomography course (13–17 October 2025)

The EMBL Imaging Centre invites applications for an immersive 5-day hands-on course focused on the cryo-lamella tomography workflow — a cutting-edge technique to study cellular samples in their native environment at high resolution.

📅 Course Dates: 13 – 17 October 2025
📍 Location: EMBL Imaging Centre, Heidelberg, Germany
👥 Participants: Limited to 8
📝 Registration Deadline: 15 July 2025
💬 Announcement of Participants: 25 July 2025
💰 Registration Fee: Free (travel and accommodation must be covered by participants)

🔍 Course highlights

This course will provide theoretical foundations and practical training in:

  • Sample vitrification: Plunge- and high-pressure freezing techniques
  • Cryo-FIB milling: On-grid lamella preparation using state-of-the-art instruments
  • Cryo-electron tomography (cryo-ET): Data acquisition and high-resolution imaging
  • Computational analysis: Tomogram reconstruction, segmentation, and sub-tomogram averaging

An open equipment day will offer further exploration opportunities with expert guidance.

🎓 Lectures from leading scientists

Featuring invited talks by:

  • Dr. Petr Chlanda – Cryo-CLEM studies on Ebola virus
  • Dr. Bettina Zens – Cryo-lift-out and ECM analysis

And lectures by EMBL IC experts on sample prep, cryo-FIB milling, tomography tools, 3D modeling, and molecular-level analyses.

🛠️ Hands-On Practicals

You will gain hands-on experience with world-class instruments:

  • GP2 & EM ICE (Leica Microsystems) – Sample vitrification
  • Aquilos 2 (ThermoFisher Scientific) – Automated cryo-FIB milling
  • Titan Krios G4 (ThermoFisher Scientific) – High-resolution tomography
  • AreTomo3, MIB, SerialEM – Computational tools for 3D reconstruction and segmentation

📝 How to Apply

Interested participants must submit:

  • A CV
  • A motivation letter describing your background, access to cryo-lamella instrumentation, and how this course aligns with your research goals

📩 Apply now! Space is limited and highly competitive.

👉 https://www.embl.org/about/info/imaging-centre/basicsfibcourse2025/

For more information, contact:
Georg Wolff – georg.wolff@embl.de
Anna Steyer – anna.steyer@embl.de

Don’t miss this opportunity to master cryo-FIB milling and in situ cryo-electron tomography!

Edit