Nickels Jensen, Abberior Instruments GmbH, Hans-Adolf-Krebs-Weg 6, 37077 Göttingen
Understanding the structure and dynamics of biological systems at the molecular scale is essential for revealing the principles of cellular function. Imaging and tracking are therefore two of the most important approaches for studying biological systems: imaging reveals molecular architecture, while tracking captures dynamic processes, often occurring on rapid timescales. To address both, microscopy methods must combine molecular specificity with nanometer-scale spatial resolution and high temporal precision.
MINFLUX nanoscopy meets this challenge by enabling both the precise imaging of biological structures and the tracking of molecular dynamics with exceptional accuracy [1]. Through iterative localization, MINFLUX determines fluorophore positions with exceptional precision while maximizing the information gained from each detected photon, making it the most photon-efficient approach in super-resolution microscopy. This enables isotropic 3D localization precisions of a few nanometers and temporal resolutions down to 100 µs in tracking experiments [Fig.1].
Here, we present the MINFLUX microscope, implemented on a conventional fluorescence microscope stand to bring this powerful technology into standard experimental workflows and make it more broadly accessible for biological research [2].
Combining ultra-high localization precision with user-friendly operation, the system enables a wider community of researchers to apply MINFLUX to biological questions. In line with Abberior’s vision of science beyond barriers, this workshop offers participants the opportunity to gain deeper insight into the capabilities of MINFLUX and to explore how it is helping to broaden access to nanoscale imaging and tracking.
In addition to the symposium programme, participants are warmly invited to schedule a demonstration of MINFLUX.
