Structural characterisation of macromolecular complexes

Time (Europe/Grenoble)	Speaker
12:00 – 12:30	Arrival and registration
12:30 – 13:30	Buffet lunch
13:30 – 14:00	Opening and course overview
14:00 – 15:30	Student presentations – Part 1
15:30 – 16:00	Coffee break
16:00 – 17:30	Student presentations – Part 2
17:30 – 18:00	Break
18:00 – 18:45	Lecture 1: Structural studies of nucleosome binding complexes Song Tan – The Pennsylvania State University, USA
19:00 – 20:00	Dinner at the EPN campus

Time (Europe/Grenoble)	Speaker
08:45 – 09:00	Overview of the day Wojciech Galej – EMBL Grenoble, France
09:00 – 09:45	Lecture 2: Introduction to protein expression in E. coli Christophe Romier – Institut de génétique et de biologie moléculaire et cellulaire, France
09:45 – 10:30	Lecture 3: Eukaryotic expression systems Kim Remans – EMBL Heidelberg, Germany
10:30 – 11:00	Coffee break
11:00 – 11:45	Lecture 4: Mass spectrometry in structural biology Andrea Sinz – Martin Luther University Halle-Wittenberg, Germany
11:45 – 13:00	Lunch break
13:00 – 19:00	Social event: visit to Vizille Castle
19:00 – 21:00	Group dinner

Time (Europe/Grenoble)	Speaker
08:45 – 09:00	Overview of the day Carlo Petosa – Institut de Biologie Structurale, France
09:00 – 09:45	Lecture 5: Introduction to super-resolution microscopy Mark Bates – Institut für Nanophotonik Göttingen, Germany
09:45 – 10:30	Lecture 6: Single molecule FRET in studying proteins interactions and dynamics Sigrid Milles – Leibniz-Forschungsinstitut für Molekulare Pharmakologie im Forschungsverbund Berlin e.V. (FMP), Germany
10:30 – 11:00	Coffee break
11:00 – 11:45	Lecture 7: Introduction to small angle x-ray scattering Mark Tully – European Synchrotron Radiation Facility, France
11:45 – 13:15	Poster session 1
13:15 – 14:15	Lunch and networking
14:15 – 16:15	Parallel practical session 1 Practicals A, B, C, D Participants divided into groups of 4 attend one of the practical sessions A: EM intro (P1) B: Biophysics (P9) C: AF(P10) D: MS(P7)
16:15 – 16:45	Coffee break
16:45 – 18:45	Parallel practical session 2 Practicals A, B, C, D Participants divided into groups of 4 attend one of the practical sessions B: EM intro (P1) A: EM analysis (P2) C: Biophysics (P9) D: AF(P10)
18:45 – 19:45	Dinner

Time (Europe/Grenoble)	Speaker
08:45 – 09:00	Overview of the day Montserrat Soler Lopez – European Synchrotron Radiation Facility, France
09:00 – 09:45	Lecture 8: Introduction to automated synchrotron crystallography Matthew Bowler – EMBL Grenoble, France
09:45 – 10:30	Lecture 9: Serial X-ray crystallography Jörg Standfuss – Paul Scherrer Institut, USA
10:30 – 11:00	Coffee break
11:00 – 11:45	Lecture 10: Structure prediction and modeling of RNA Janusz Bujnicki International Institute of Molecular and Cell Biology in Warsaw, Poland
11:45 – 12:30	Lecture 11: Integrative structural biology of RNA-protein complexes Teresa Carlomagno – University of Birmingham, UK
12:30 – 13:30	Lunch
13:30 – 15:30	Parallel practical session 3 Practicals A, B, C, D Participants divided into groups of 4 attend one of the practical sessions D: Biophysics (P9) C: EM intro (P1) B: EM analysis (P2) A: AF(P10)
15:30 – 16:00	Coffee break
16:00 – 18:00	Parallel practical session 4 Practicals A, B, C, D Participants divided into groups of 4 attend one of the practical sessions A: Biophysics (P9) D: EM intro (P1) C: EM analysis (P2) B: MS(P7)
18:00 – 22:00	Get together with PSB scientists Networking event with local PSB scientists with buffet dinner and drinks

Time (Europe/Grenoble)	Speaker
08:45 – 09:00	Overview of the day Wojciech Galej – EMBL Grenoble, France
09:00 – 09:45	Lecture 12: Introduction to cryo-EM Rebecca Thompson – Thermo Fisher Scientific, UK
09:45 – 10:30	Lecture 13: Cryo-electron tomography Julia Mahamid – EMBL Heidelberg, Germany
10:30 – 11:00	Coffee break
11:00 – 12:30	Poster session 2
13:30 – 15:30	Parallel practical session 5 Practicals A+B, C, D Participants divided into groups of 4 attend one of the practical sessions A+B:NMR (P3) C: MS(P7) D: EM analysis (P2)
15:30 – 16:00	Coffee break
16:00 – 18:00	Parallel practical session 6 Practicals C+D, A, B Participants divided into groups of 4 attend one of the practical sessions C+D:NMR (P3) A: MS(P7) B: AF(P10)
18:00	Free evening

Time (Europe/Grenoble)	Speaker
08:45 – 09:00	Overview of the day Eva Kowalisnki – EMBL Grenoble, France
09:00 – 09:45	Lecture 14: Atomic Force Microscopy Luca Costa – Centre de Biologie Structurale, France
09:45 – 10:30	Lecture 15: Introduction to NMR spectroscopy Cameron Mackereth – University of Bordeaux, France
10:30 – 11:00	Coffee break
11:00 – 11:45	Lecture 16: Biophysical characterisation of protein complexes Carolina Mas – Institut de Biologie Structurale, France
11:45 – 12:30	Lecture 17: Talk title TBC Martin Steinegger – Seoul National University, South Korea
12:30 – 13:30	Lunch
13:30 – 15:30	Parallel practical session 7 Practicals A+B, C, D Participants divided into groups of 4 attend one of the practical sessions A+B: Serial prep (P5) C: SAXS (P4) D: XL-MS (P8)
15:30 – 16:00	Coffee break
16:00 – 18:00	Parallel practical session 8 Practicals A+B, C, D Participants divided into groups of 4 attend one of the practical sessions A+B: Serial-MX(P6) D: SAXS (P4) C: XL-MS (P8)
18:00	Dinner

Time (Europe/Grenoble)	Speaker
08:45 – 09:00	Overview of the day Wojciech Galej – EMBL Grenoble, France
09:00 – 09:45	Lecture 18: iNEXT-discovery and and large infrastructures for structural biology Anastassis (Tassos) Perrakis – Netherlands Cancer Institut, The Netherlands
09:45 – 10:30	Lecture 19: Opportunities and challenges of AI methods in structural biology Randy Read – University of Cambridge, UK
10:30 – 11:00	Coffee break
11:00 – 11:45	Lecture 20: Mechanistic studies of the RNA surveillance pathways Elena Conti – Max Planck Institute of Biochemistry, Germany
11:45 – 12:30	Panel discussion of responsible conduct of research
12:30 – 13:30	Lunch
13:30 – 15:30	Parallel practical session 9 Practicals A, B, C+D Participants divided into groups of 4 attend one of the practical sessions C+D: Serial prep (P5) A: SAXS (P4) B: XL-MS (P8)
15:30 – 16:00	Coffee break
16:00 – 18:00	Parallel practical session 10 Practicals A, B, C+D Participants divided into groups of 4 attend one of the practical sessions C+D: Serial-MX(P6) B: SAXS (P4) A: XL-MS (P8)
18:00 – 18:30	Break
18:30 – 19:15	Lecture 21: Structural data visualisation Janet Iwasa – University of Utah, USA (Virtual speaker)
19:15 – 21:00	Gala dinner

Time (Europe/Grenoble)	Speaker
08:45 – 09:00	Overview of the day Carlo Petosa – Institut de Biologie Structurale, France
09:00 – 09:45	Lecture 22: X-ray imaging in structural biology Alexandra Pacureanu – European Synchrotron Radiation Facility, France
09:45 – 10:30	Lecture 23: Phase separation and membraneless organelles Maria Hondele – The Biozentrum, University of Basel, Switzerland
10:30 – 11:00	Coffee break
11:00 – 11:45	Lecture 25: Structural studies of RNA protein complexes Wojtek Galej, EMBL Grenoble, France
11:45 – 12:30	Course evaluation
12:30 – 13:30	Lunch
13:30	Departure

The course is limited to 20 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.

Registration

Registration fees include accommodation (location to be confirmed), all meals throughout the duration of the course, admission and course materials. Travel expenses are NOT covered with the registration fee.

Academia	€500
PhD Student	€500
Industry	€1000

A letter to support your visa application will be issued, on request, once payment of the registration fee is confirmed. We recommend that you book your visa appointment as soon as possible, to avoid any delay with your visa application.

Confirmation and payment

The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.

Motivation letter submission

After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.

Instructions

1. Include your relevant skills, experience and qualifications showing why you would be suitable for the course.
2. Explain why you would like to attend, including what you can contribute and how you think you will benefit from the course.

Please note:

• The limit of 2000 characters refers to manually typed text and excludes spaces. If an error occurs try using a different web browser (preferably Google Chrome or Mozilla Firefox).
• If you copy-paste the text into the form, hidden formatting might still be included which may cause the text to exceed the 2,000 character limit resulting in an error message. We recommend you clear all formatting before pasting in the text.
• If you have special symbols in your text, make sure you are using Unicode characters, otherwise these will not be recognised.

For detailed instructions, please watch our video on how to submit a course motivation letter.

Further details

For further information about registration and motivation letter submission please refer to the FAQ page.

Financial assistance

Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of registration fee waivers, travel grants and childcare grants. 

Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.

Registration fee waiver

The fee waiver will cover the registration sum that you have paid to attend the course.

Travel grant

The travel grant will cover the cost of travel (airfare, train, bus, taxi, accommodation, visa, and/or registration fees*) and is provided up to specified caps which are normally as follows:

– up to €400 for participants travelling to an EMBL Course within Europe.

– up to €1000 for participants travelling to an EMBL Course from outside Europe.

– up to €500 for any participant travelling to an EMBO Practical Course.

– up to €1000 for any participant working in Chile, India, Singapore or Taiwan travelling to an EMBO Practical Course.

– up to €700 for any participant working in Croatia, Czech Republic, Estonia, Greece, Hungary, Italy, Lithuania, Luxembourg, Poland, Slovenia, and Turkey travelling to an EMBO Practical Course.

*Registration fees are only covered for EMBO Practical Courses

The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.

Childcare grant

There is the possibility to apply for a childcare grant to offset child care costs incurred by participants, speakers, trainers and organisers when attending a course. Eligible costs include (but are not limited to) fees for a babysitter or child-care facility and travel costs for a caregiver. Please note that priority will be given to early-stage researchers. There is a limited amount of funding available for the childcare grants and funds will be distributed amongst eligible applicants.

Boehringer Ingelheim Fonds travel grant

Applies to selected courses only. Availability will be indicated during the motivation letter submission process.

This grant covers costs related to your attendance at the course (registration, travel and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.

Whether you are eligible to apply for a travel grant, depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):

– for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course

– for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course

Application

You may apply for financial assistance when submitting your motivation letter for courses. In your application, you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.

For the Boehringer Ingelheim Fonds Travel Grant, there is a pre-application question during the motivation letter submission process, and if selected you will be requested to complete a standard form and documentation consisting of your travel expense estimation.

Selection

The scientific organisers will select the recipients of registration fee waivers and travel grants during the motivation letter or abstract selection process. Results will be announced approximately 6 – 8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the meeting. Selection for registration fee waivers and travel grants is based on scientific merit, your current work or study location, the reasons for needing financial support, and the impact this event will have on your career.

Childcare grants will be allocated in the same timeframe (6-8 weeks before the event start date). Please note that priority will be given to early-stage researchers.

Reimbursement

Costs will be reimbursed after the meeting only once a reimbursement form and original receipts (from travel costs) have been received.

Further details

See our list of external funding opportunities and for further information about financial assistance please refer to the FAQ page.

Hotel

Reservations have been made for course participants at accommodation near the EMBL Grenoble site. More details will be available soon. Note, accommodation is included in the registration fee. 

Local transport

Shuttle buses will go from the hotel to EMBL campus and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.

Further details

Address: EMBL Grenoble, 71 Av. des Martyrs, 38000 Grenoble, France

From Lyon-Saint Exupéry Airport take the airport shuttle bus Lyon-Saint Exupéry–Grenoble counter (Satobus) inside the airport or in the bus. A single ticket costs 22€, a return ticket 33€.The stop “Place de la Résistance” is within easy walking distance of the EMBL site.

From Grenoble take Tram B towards “Resistance roundabout” and exit at “Grenoble Presquile”. The site entry is 350m away from the stop.

For further information on getting to EMBL Grenoble visit our Travel Information page.

Technical requirements

Information will be provided ahead of the course about installing any software required.

Event software

The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus. 

Updated information will be provided closer to the time as required.