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Structural characterisation of macromolecular complexes – Course and Conference Office

EMBO Practical Course

Structural characterisation of macromolecular complexes

Overview
Sponsors

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Course overview

Recent developments in cryo-EM/ET, super-resolution microscopy, and protein structure prediction — coupled with increasingly sophisticated methods of sample preparation — have revolutionised structural biology. Educating the next generation of researchers on how to strategically combine these disparate tools is critical for addressing the most challenging structural biology problems of the future.

Course participants will receive a comprehensive overview of state-of-the-art structural methods, with a focus on sample preparation, characterisation, and data integration strategies. Key aspects covered by lectures and practicals include multi-subunit protein expression in bacteria, insect, and mammalian cells; isolation of native complexes and in-vitro reconstitution of protein and protein-nucleic acid complexes; biochemical and biophysical characterization to assess stoichiometry and sample homogeneity; 3D structure determination of macromolecular assemblies at different resolution regimes; and the integration of data from multiple experimental sources (X-ray crystallography, cryo-EM, SAXS, NMR, mass spectrometry, smFRET, and super-resolution light microscopy). The current edition of this long-standing course refreshes the portfolio of speakers and topics, including a new module on the strategic exploitation of the latest protein structure prediction methods.

Sponsors

Sponsorship opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other events, or contact sponsorship@embl.de for further information.

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