Proof of COVID-19 vaccination or recovery is required to attend this on-site course. Please see EMBL’s COVID-19 safety policy for on-site events.
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This practical course will cover advanced light microscopy techniques and participants will learn how to derive qualitative and quantitative insights on molecular mechanisms in cells and developing organisms. Invited guest researchers together with experts from the EMBL and microscopy professionals will foster an intense information flow with a balance of lectures and practical workshops. The focus of the course will be on the use of fluorescence microscopy to obtain information about protein localisation, dynamics, and functions at different spatial scales.
This course is directed towards researchers in the life sciences with access to light microscopy equipment, who have an immediate need to apply the techniques they will learn to solve biological problems at the cellular and molecular level in vivo.
The topics will include fluorescence microscopy techniques such as fast time-lapse microscopy, spinning disk and point-scanning confocal microscopy, light sheet microscopy of cleared samples, photo-manipulation as a tool, multicolor single molecule localization microscopy (SMLM) with spectral demixing. For the demanding applications we will cover the aspects of image analysis and possibilities of microscope automation. The modules will cover all necessary knowledge and demonstrate tools to conduct similar pipelines at your home lab enabling you to answer emerging biological questions.
Participants will get an overview about advanced fluorescence imaging techniques.
This course is kindly supported by the EMBL Advanced Light Microscopy Facility.
This course is organised in cooperation with Evident-Olympus.
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