Super-resolution microscopy: time-resolved STED nanoscopy – Course and Conference Office

EMBL Course

Super-resolution microscopy: time-resolved STED nanoscopy


Proof of COVID-19 vaccination or recovery is required to attend this on-site course. Please see EMBL’s COVID-19 safety policy for on-site events.

Registration is not yet open for this event. If you are interested in receiving more information please register your interest.

Course Overview

This six-day comprehensive microscopy course focuses on stimulated emission depletion microscopy (STED) and its application to biological questions. It covers the fundamentals of STED microscopy, different technical implementations and has a strong emphasis on sample preparation – including choice of fluorophores and refractive index matching, as well as image acquisition strategies for fixed samples and living cells. Students will have the opportunity for exercises on state-of-the-art STED microscopes in which they will acquire high quality nanoscopy data. Through lectures, tutorials, demonstrations and practical workshops, the course provides in-depth knowledge on all aspects of STED Nanoscopy.


This course is designed for researchers at the early stages of their career in the life sciences (e.g. pre or postdoctoral level), ideally with access to a STED microscope. The participants should have an immediate need to apply the trained skills for their biological questions. The course is strongly centered on practical aspects of STED microscopy and is therefore especially suited for people who rely on STED nanoscopy for their research.


The course will cover the entire range of skills needed to apply STED nanoscopy in biology. The following topics will be covered in lectures, tutorials as well as in practical workshops:

  • Sample preparation for STED microscopy, including choice of fluorophores and refractive index matching
  • Technical implementations – CW-STED, pulsed-STED, gated-STED, time-resolved STED (tauSTED)
  • Multi-Color 2D and 3D STED microscopy
  • Image acquisition strategies for live-cell STED
  • STED Fluorescence Correlation Spectroscopy (STED-FCS)

Learning Outcomes

At the end of the course, the participants will be able to design experiments using STED microscopy, prepare high-quality microscopy samples and acquire state-of-the-art nanoscopy images.

This course is organised in cooperation with Leica Microsystems.


Sponsorship Opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other events, or contact sponsorship@embl.de for further information.

Date: 11 - 16 Jul 2022

Location: EMBL Heidelberg

Venue: EMBL Advanced Training Centre


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