Hands-on flow cytometry: learning by doing!

Overview

Please see EMBL’s COVID-19 safety policy for testing and other requirements to attend this on-site course.

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Course overview

Flow Cytometry is one of the most powerful single cell analysis methods used throughout the life sciences and medicine. By utilising Flow Cytometry we can identify and quantify specific cell populations from heterogeneous samples, analyse the cell cycle or proliferation state or simply detect the expression of fluorescent reporters. In line with the suggestions from our course in 2019, we will significantly enhance the number of analysis sessions together with our partners from FlowJo. We will cover the theory of Flow Cytometry and corroborate this information intensively with the practical issues of the most frequent assays as part of our practical course curriculum.

Audience

The course is designed for researchers at any step of their career and will cover the subject from basic principles to complex assays. Prior basic working knowledge of Flow Cytometry, data acquisition or analysis software (DIVA + FlowJo)  is of advantage, but not strictly a must.

Modules/resources

• In-depth principles of Flow Cytometry
• Quality assessment and instrument validation
• Analysis of cell cycle profiles in fixed and live cells
• Proliferation and cell division assessment
• Apoptosis assays
• Tissue staining of cell surface and intracellular antigens
• Principles and guidelines for experimental design, especially for multi-colour Flow Cytometry
• Essential of gating and gating controls
• Detailed multi-sessions on analysing data from Flow Cytometers in cooperation with FlowJo
• Guidelines to prepare flow cytometry data for publication 

Learning outcomes

Our goal for this course is to teach the basic of this technology with a very big emphasis on practical work at the instruments. Nothing is better than learning by doing. We have a great set of experienced trainers in this technology delivering lectures and accompanying you into the lab doing flow on your own. We will have support from FlowJo delivering the basics and some advanced features in multiple sessions. This in-depth five-day course will push your knowledge and skills preparing you for any flow projects ahead on your research path.

What past participants say about the course

“My experience at EMBL was lovely and stimulating. The knowledge I acquired through this short time has improved my learning and practical skills. EMBL is a great place for scientific resources; a friendly environment both socially and professionally.” – Ibrahim Malami, Usmanu Danfodiyo University Sokoto

“The course was excellently organized, with high quality theoretical and practical sessions! It is a must for everyone interested in basic and more specific aspects of flow cytometry techniques.” – Filipe Olim, University of Madeira

“I enjoyed the course a lot and learned what I was looking for: an opportunity to learn the principles of flow cytometry that are important for planning and performing experiments. Besides the theoretical part, I was able to run samples at the instruments by myself which was a great starting point to perform experiments by myself in the future.” – Maya Funk, German Cancer Research Center

Sponsors

Sponsorship opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other events, or contact sponsorship@embl.de for further information.

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