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Advances in cryo-electron microscopy and 3D image processing – Course and Conference Office

EMBO Practical Course

Advances in cryo-electron microscopy and 3D image processing

Overview

Course overview

Technology advances in cryo-electron microscopy (cryoEM) over the last decade have transformed our understanding of biomolecular structures across different spatial scales. CryoEM has unique capabilities for obtaining structural information in situ, within complex (cellular) environments, and to obtain atomic-level structures of biologically flexible and heterogeneous macromolecular assemblies. With the advent of the cryoEM ‘resolution revolution’, the method has undergone a tremendous growth in user base. Yet, emerging breakthroughs, such as new (correlated) imagine technologies and in AI-based image processing, demonstrate the potential for cryoEM to make another leap forward towards new frontiers. Therefore, in depth advanced training is essential to ensure future generations can adapt such emerging technologies to expand into new areas of biological research. This course provides the theoretical and practical foundations of advanced cryoEM techniques, from sample preparation to data collection to image processing.

Audience

To meet the exceptional demand of the field for advanced training, this course is aimed at PhD students and postdocs with basic experience in cryoEM and 3D image processing.

Modules/resources

During this course we will focus on the following aspects:

  • Single particle reconstruction (cisTEM, Relion, cryoSPARC)
  • Subtomogram averaging
  • Atomic models (model building and integrative modeling)
  • Microscopy (cryoEM/ET and cryo-FIB)
  • Latest developments in instrumentations

Learning outcomes

The course will enable participants to seamlessly integrate single particle analysis and tomography approaches to their cutting-edge research, disseminate their gained expertise to colleagues and, eventually, to innovate cryoEM methods beyond the state-of-the-art.

What past participants say about the course

This a world-class course. The access to experts and cutting-edge techniques is incredible.” – Corbin Black, McGill University, Canada

 

The EMBL Imaging Centre training activities are generously supported by the Boehringer Ingelheim Foundation.

Speakers

Speakers, trainers, and staff

Alister Burt

Genentech

USA

Bridget Carragher

Chan Zuckerberg Imaging Institute

USA

(Virtual speaker)

Robert Glaeser

Lawrence Berkeley National Laboratory

USA

(Virtual speaker)

Michal Grzadkowski

Princeton University

USA

Hui Guo

Max Planck Institute of Biochemistry

Germany

Dari Kimanius

Chan Zuckerberg Imaging Institute

USA

Sjors Scheres

MRC Laboratory of Molecular Biology

UK

(Virtual speaker)

Dimitry Tegunov

Genentech

USA

Scientific Organisers

Conference Organisers

Irena Provaznikova

Scientific Training Manager

EMBL Heidelberg

Germany

Aline Cecilie Schnieder

Course and Conference Manager

EMBL Heidelberg

Germany

Preliminary programme

Are you on social media? Post using #EMBOCryoEM and don’t forget to tag @EMBLEvents.

  • Some pre-course work (such as watching pre-recordings, protocol reading and computational exercises) with estimated time 0-10 hours will be required and will be shared in advance of the course.
  • Some speakers may need to join virtually to give their lecture or Q&A, and the programme is subject to change.
Day 0 – Monday 8 July 2024
Time
(Europe/Berlin)
ProgrammeLocation
12:00 – 14:00Virtual onboarding session and introduction to EMBL e-Campus and how to view recorded lecturesVirtual
Day 1 – Sunday 18 August2024
Time
(Europe/Berlin
ProgrammeLocation
15:00 – 15:40Registration and refreshments
15:40 – 15:50Introduction and housenote slides
Sebastian Eustermann – EMBL Heidelberg, Germany
15:50 – 16:00Course overview
16:00 – 17:00Student flash talks
17:00 – 17:30Coffee break
17:30 – 18:30Student flash talks
18:30 – 19:45Remote Lecture 1: Past and future of cryoEM
Robert Glaeser – Lawrence Berkeley National Laboratory, USA (virtual speaker)
19:45 – 20:45DinnerISG Hotel
Day 2 – Monday 19 August 2024
Time
(Europe/Berlin
ProgrammeLocation
08:40 – 08:45Overview of the day
08:45 – 09:05Industry talk: Emerging technologies from leading industry experts
09:05 – 10:20Lecture: Cryo-EM image formation and optics
Chris Russo – MRC Laboratory of Molecular Biology, UK
10:20 – 10:50Coffee break
10:50 – 12:05Lecture: Next generation: microscope hardware
Carsten Sachse – Forschungszentrum Jülich, Germany
12:05 – 13:05Lunch break
13:05 – 14:20(Remote) Lecture: Next generation: cryoEM single particle sample preparation & acquisition
Lori Passmore – MRC Laboratory of Molecular Biology, UK
14:20 – 15:35Lecture: cryoET sample preparation & acquisition
Julia Mahamid – EMBL Heidelberg, Germany
15:35 – 16:05Coffee break
16:05 – 17:20Remote Lecture: Fundamentals in image processing: Maximum likelihood
Sjors Scheres – MRC Laboratory of Molecular Biology, UK
17:20 – 18:35Lecture: Fundamentals in image processing: cryoET
Tanmay Bharat – MRC Laboratory of Molecular Biology, UK
18:35 – 19:35DinnerEMBL Canteen
Day 3 – Tuesday 20 August 2024
Time
(Europe/Berlin
ProgrammeLocation
08:40 – 08:45Overview of the day
08:45 – 09:05Industry talk: Emerging technologies from leading industry experts
09:05 – 10:20Next generation: High resolution by cryo ET subtomogram averaging
Dimitry Tegunov – Genentech, USA
10:20 – 10:40Coffee break
10:40 – 11:55Next generation: 2D template matching and beyond
Nikolaus Grigorieff – University of Massachusetts Medical School, USA
11:55 – 12:25Panel discussion: Hardware and sample preparation
Panel leaders: Simone Mattei and Julia Mahamid – EMBL Heidelberg, Germany
12:25 – 13:25Lunch break
13:25 – 15:55Practical session A

Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic

Dari Kimanius – Chan Zuckerberg Imaging Institute, USA
Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic
Hui Guo – Max Planck Institute of Biochemistry, Germany
Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic
Tanmay Bharat – MRC Laboratory of Molecular Biology, UK
Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK
Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic
Dimitry Tegunov – Genentech, USA
Joe Dobbs – EMBL Heidelberg, Germany
Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany
Session 5 on Model building, refinement and validation
Sebastian Eustermann – EMBL Heidelberg, Germany
15:55 – 16:25Coffee break
16:25 – 18:55Practical session B

Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.)

Dari Kimanius – Chan Zuckerberg Imaging Institute, USA
Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.)
Hui Guo – Max Planck Institute of Biochemistry, Germany
Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.)
Tanmay Bharat – MRC Laboratory of Molecular Biology, UK
Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK
Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.)
Dimitry Tegunov – Genentech, USA
Joe Dobbs – EMBL Heidelberg, Germany
Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany
Session 5 on Model building, refinement and validation (cont.)
Sebastian Eustermann – EMBL Heidelberg, Germany
18:55 – 19:55Canteen dinner
19:55 – 20:55Remote Lecture: Hot topics: recent breakthroughs and emerging technologies
Bridget Carragher – Chan Zuckerberg Imaging Institute, USA
20:55 – 21:25Discussion round: Hot topics: recent breakthroughs and emerging technologies
Day 4 – Wednesday 21 August 2024
Time
(Europe/Berlin
ProgrammeLocation
08:40 – 08:45Overview of the day
08:45 – 09:05Industry talk: Emerging technologies from leading industry experts
09:05 – 10:20Lecture: Advances in cryoET particle identification
Beata Turonova – Max Planck Institute of Biophysics, Germany
10:20 – 11:35Lecture: AI-based methods: Molecular heterogeneity landscapes from cryoEM and cryoET
Ellen Zhong – Princeton University, USA
11:35 – 11:55Coffee break
11:55 – 12:25Panel discussion: Software and image processing
Panel leaders: Sebastian Eustermann – EMBL Heidelberg, Germany and Tanmay Bharat – MRC Laboratory of Molecular Biology, UK
12:25 – 13:25Lunch break
13:25 – 15:55Practical session C

Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.)

Dari Kimanius – Chan Zuckerberg Imaging Institute, USA
Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.)
Hui Guo – Max Planck Institute of Biochemistry, Germany
Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.)
Tanmay Bharat – MRC Laboratory of Molecular Biology, UK
Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK
Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.)
Dimitry Tegunov – Genentech, USA
Joe Dobbs – EMBL Heidelberg, Germany
Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany
Session 5 on Model building, refinement and validation (cont.)
Sebastian Eustermann – EMBL Heidelberg, Germany
15:55 – 16:25Coffee break
16:25 – 18:55Practical session D

Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.)

Dari Kimanius – Chan Zuckerberg Imaging Institute, USA
Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.)
Hui Guo – Max Planck Institute of Biochemistry, Germany
Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.)
Tanmay Bharat – MRC Laboratory of Molecular Biology, UK
Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK
Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.)
Dimitry Tegunov – Genentech, USA
Joe Dobbs – EMBL Heidelberg, Germany
Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany
Session 5 on Model building, refinement and validation (cont.)
Sebastian Eustermann – EMBL Heidelberg, Germany
18:55 – 19:55Pizza dinner
19:55 – 20:55Poster session with drinks (12 posters)
Day 5 – Thursday 22 August 2024
Time
(Europe/Berlin
ProgrammeLocation
08:40 – 08:45Overview of the day
08:45 – 09:05Industry talk: Emerging technologies from leading industry experts
09:05 – 10:05Lecture: Next generation: Atomic model building, refinement and validation
Sebastian Eustermann – EMBL Heidelberg, Germany
10:05 – 11:05Lecture: Next generation: integrative model building
Jan Kosinski – EMBL Hamburg, Germany
11:05 – 11:35Coffee break
11:35 – 12:35EMDB and EMPIAR Data repositories
Kyle Morris – EMBL-EBI, UK
12:35 – 13:05Panel discussion: Validation and FAIR data
Panel leader: Lori Passmore – MRC Laboratory of Molecular Biology, UK and Sebastian Eustermann – EMBL Heidelberg, Germany
13:05 – 14:05Lunch break
14:05 – 16:35Practical session E

Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.)

Dari Kimanius – Chan Zuckerberg Imaging Institute, USA
Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.)
Hui Guo – Max Planck Institute of Biochemistry, Germany
Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.)
Tanmay Bharat – MRC Laboratory of Molecular Biology, UK
Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK
Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.)
Dimitry Tegunov – Genentech, USA
Joe Dobbs – EMBL Heidelberg, Germany
Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany
Session 5 on Model building, refinement and validation (cont.)
Sebastian Eustermann – EMBL Heidelberg, Germany
Session 6 on Macromolecular Heterogeneity Analysis | cryoDRGN topic (cont.)
Ellen Zhong – Princeton University, USA
Session 7 2D template matching (cont.)
Nikolaus Grigorieff – University of Massachusetts Medical School, USA
16:35 – 17:05Coffee break
17:05 – 19:35Practical session F

Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.)

Dari Kimanius – Chan Zuckerberg Imaging Institute, USA
Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.)
Hui Guo – Max Planck Institute of Biochemistry, Germany
Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.)
Tanmay Bharat – MRC Laboratory of Molecular Biology, UK
Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK
Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.)
Dimitry Tegunov – Genentech, USA
Joe Dobbs – EMBL Heidelberg, Germany
Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany
Session 5 on Model building, refinement and validation (cont.)
Sebastian Eustermann – EMBL Heidelberg, Germany
Session 6 on Macromolecular Heterogeneity Analysis | cryoDRGN topic
Ellen Zhong – Princeton University, USA
Session 7 2D template matching
Nikolaus Grigorieff – University of Massachusetts Medical School, USA
Johannes Elferich – University of Massachusetts Medical School, USA
19:35 – 20:35Dinner
20:35 – 21:35Poster session with drinks (12 posters)
Day 6 – Friday 23 August 2024
Time
(Europe/Berlin
ProgrammeLocation
08:40 – 08:45Overview of the day
08:45 – 09:05Industry talk: Emerging technologies from leading industry experts
09:05 – 10:20High Throughput and Resolution Data collection
Simone Mattei – EMBL Heidelberg, Germany
10:20 – 11:35Correlative light and electron microscopy for Structural Cell Biology
Wanda Kukulski – University of Bern, Switzerland
11:35 – 11:55Coffee break
11:55 – 12:25Panel discussion: Data acquisition
Panel leader: Simone Mattei – EMBL Heidelberg, Germany
12:25 – 13:25Lunch break
13:25 – 15:55Practical Session G

Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.)

Dari Kimanius – Chan Zuckerberg Imaging Institute, USA
Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.)
Hui Guo – Max Planck Institute of Biochemistry, Germany
Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.)
Tanmay Bharat – MRC Laboratory of Molecular Biology, UK
Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK
Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.)
Dimitry Tegunov – Genentech, USA
Alister Burt – Genentech, USA
Joe Dobbs – EMBL Heidelberg, Germany
Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany
Session 5 on Model building, refinement and validation (cont.)
Sebastian Eustermann – EMBL Heidelberg, Germany
Session 6 on Macromolecular Heterogeneity Analysis | cryoDRGN topic (cont.)
Ellen Zhong – Princeton University, USA
Session 7 2D template matching (cont.)
Nikolaus Grigorieff – University of Massachusetts Medical School, USA
Johannes Elferich – University of Massachusetts Medical School, USA
15:55 – 16:25Coffee break
16:25 – 18:55Practical Session H

Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.)

Dari Kimanius – Chan Zuckerberg Imaging Institute, USA
Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.)
Hui Guo – Max Planck Institute of Biochemistry, Germany
Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.)
Tanmay Bharat – MRC Laboratory of Molecular Biology, UK
Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK
Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.)
Dimitry Tegunov – Genentech, USA
Alister Burt – Genentech, USA
Joe Dobbs – EMBL Heidelberg, Germany
Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany
Session 5 on Model building, refinement and validation (cont.)
Sebastian Eustermann – EMBL Heidelberg, Germany
Session 6 on Macromolecular Heterogeneity Analysis | cryoDRGN topic (cont.)
Ellen Zhong – Princeton University, USA
Session 7 2D template matching (cont.)
Nikolaus Grigorieff – University of Massachusetts Medical School, USA
Johannes Elferich – University of Massachusetts Medical School, USA
18:55 – 19:55Dinner in EMBL Canteen
19:55 – 20:25Lecture: cryoEM careers in academia and pharma
Dimitry Tegunov – Genentech, USA
20:25 – 21:25Discussion round: Career paths
Day 7 – Saturday 24 August 2024
Time
(Europe/Berlin
ProgrammeLocation
08:40 – 08:45Overview of the day
08:45 – 12:05Practical Session I – Drop in session

Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.)

Dari Kimanius – Chan Zuckerberg Imaging Institute, USA
Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.)
Hui Guo – Max Planck Institute of Biochemistry, Germany
Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.)
Tanmay Bharat – MRC Laboratory of Molecular Biology, UK
Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK
Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.)
Dimitry Tegunov – Genentech, USA
Alister Burt – Genentech, USA
Joe Dobbs – EMBL Heidelberg, Germany
Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany
Session 5 on Model building, refinement and validation (cont.)
Sebastian Eustermann – EMBL Heidelberg, Germany
Session 6 on Macromolecular Heterogeneity Analysis | cryoDRGN topic (cont.)
Ellen Zhong – Princeton University, USA
Session 7 2D template matching (cont.)
Nikolaus Grigorieff – University of Massachusetts Medical School, USA
Johannes Elferich – University of Massachusetts Medical School, USA
Session 8 on Cryo-TEM
Simon Fromm – EMBL Heidelberg, Germany
Session 9 on Cryo-LM/FIB-SEM
Zhengyi Yang – EMBL Heidelberg, Germany
Session 10 on HPF/Waffle prep
Trainer tbc
12:05 – 13:05Lunch break
13:05 – 15:05Practical Session J – Drop in session

Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.)

Dari Kimanius – Chan Zuckerberg Imaging Institute, USA
Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.)
Hui Guo – Max Planck Institute of Biochemistry, Germany
Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.)
Tanmay Bharat – MRC Laboratory of Molecular Biology, UK
Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK
Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.)
Dimitry Tegunov – Genentech, USA
Alister Burt – Genentech, USA
Joe Dobbs – EMBL Heidelberg, Germany
Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany
Session 5 on Model building, refinement and validation (cont.)
Sebastian Eustermann – EMBL Heidelberg, Germany
Session 6 on Macromolecular Heterogeneity Analysis | cryoDRGN topic (cont.)
Ellen Zhong – Princeton University, USA
Session 7 2D template matching (cont.)
Nikolaus Grigorieff – University of Massachusetts Medical School, USA
Johannes Elferich – University of Massachusetts Medical School, USA
Session 8 on Cryo-TEM
Simon Fromm – EMBL Heidelberg, Germany
Session 9 on Cryo-LM/FIB-SEM
Zhengyi Yang – EMBL Heidelberg, Germany
Session 10 on HPF/Waffle prep
Trainer tbc
15:05 – 15:25Coffee break
15:25 – 17:25Practical Session K – Drop in session

Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.)

Dari Kimanius – Chan Zuckerberg Imaging Institute, USA
Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.)
Hui Guo – Max Planck Institute of Biochemistry, Germany
Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.)
Tanmay Bharat – MRC Laboratory of Molecular Biology, UK
Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK
Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.)
Dimitry Tegunov – Genentech, USA
Alister Burt – Genentech, USA
Joe Dobbs – EMBL Heidelberg, Germany
Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany
Session 5 on Model building, refinement and validation (cont.)
Sebastian Eustermann – EMBL Heidelberg, Germany
Session 6 on Macromolecular Heterogeneity Analysis | cryoDRGN topic (cont.)
Ellen Zhong – Princeton University, USA
Session 7 2D template matching (cont.)
Nikolaus Grigorieff – University of Massachusetts Medical School, USA
Johannes Elferich – University of Massachusetts Medical School, USA
Session 8 on Cryo-TEM
Simon Fromm – EMBL Heidelberg, Germany
Session 9 on Cryo-LM/FIB-SEM
Zhengyi Yang – EMBL Heidelberg, Germany
Session 10 on HPF/Waffle prep
Trainer tbc
17:25 – 19:25Free time downtown
19:25 – 21:25Dinner in city centre
Day 8 – Sunday 25 August 2024
Time
(Europe/Berlin
ProgrammeLocation
10:00 – 10:05Overview of the day
10:05 – 12:05Practical Session L – Drop in session

Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.)

Dari Kimanius – Chan Zuckerberg Imaging Institute, USA
Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.)
Hui Guo – Max Planck Institute of Biochemistry, Germany
Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.)
Tanmay Bharat – MRC Laboratory of Molecular Biology, UK
Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK
Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.)
Dimitry Tegunov – Genentech, USA
Alister Burt – Genentech, USA
Joe Dobbs – EMBL Heidelberg, Germany
Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany
Session 5 on Model building, refinement and validation (cont.)
Sebastian Eustermann – EMBL Heidelberg, Germany
Session 6 on Macromolecular Heterogeneity Analysis | cryoDRGN topic (cont.)
Ellen Zhong – Princeton University, USA
Session 7 2D template matching (cont.)
Nikolaus Grigorieff – University of Massachusetts Medical School, USA
Johannes Elferich – University of Massachusetts Medical School, USA
Session 8 on Cryo-TEM
Simon Fromm – EMBL Heidelberg, Germany
Session 9 on Cryo-LM/FIB-SEM
Zhengyi Yang – EMBL Heidelberg, Germany
12:05 – 13:05Lunch break
13:05 – 16:05Practical Session M – Drop in session

Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.)

Dari Kimanius – Chan Zuckerberg Imaging Institute, USA
Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.)
Hui Guo – Max Planck Institute of Biochemistry, Germany
Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.)
Tanmay Bharat – MRC Laboratory of Molecular Biology, UK
Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK
Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.)
Dimitry Tegunov – Genentech, USA
Alister Burt – Genentech, USA
Joe Dobbs – EMBL Heidelberg, Germany
Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany
Session 5 on Model building, refinement and validation (cont.)
Sebastian Eustermann – EMBL Heidelberg, Germany
Session 6 on Macromolecular Heterogeneity Analysis | cryoDRGN topic (cont.)
Ellen Zhong – Princeton University, USA
Session 7 2D template matching (cont.)
Nikolaus Grigorieff – University of Massachusetts Medical School, USA
Johannes Elferich – University of Massachusetts Medical School, USA
Session 8 on Cryo-TEM
Simon Fromm – EMBL Heidelberg, Germany
Session 9 on Cryo-LM/FIB-SEM
Zhengyi Yang – EMBL Heidelberg, Germany
16:05 – 16:35Coffee break
16:35 – 18:35Practical Session N – Drop in session

Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.)

Dari Kimanius – Chan Zuckerberg Imaging Institute, USA
Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.)
Hui Guo – Max Planck Institute of Biochemistry, Germany
Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.)
Tanmay Bharat – MRC Laboratory of Molecular Biology, UK
Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK
Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.)
Dimitry Tegunov – Genentech, USA
Alister Burt – Genentech, USA
Joe Dobbs – EMBL Heidelberg, Germany
Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany
Session 5 on Model building, refinement and validation (cont.)
Sebastian Eustermann – EMBL Heidelberg, Germany
Session 6 on Macromolecular Heterogeneity Analysis | cryoDRGN topic (cont.)
Ellen Zhong – Princeton University, USA
Session 7 2D template matching (cont.)
Nikolaus Grigorieff – University of Massachusetts Medical School, USA
Johannes Elferich – University of Massachusetts Medical School, USA
Session 8 on Cryo-TEM
Simon Fromm – EMBL Heidelberg, Germany
Session 9 on Cryo-LM/FIB-SEM
Zhengyi Yang – EMBL Heidelberg, Germany
18:35 – 19:50Lecture: Future of cell structural biology
Julia Mahamid – EMBL Heidelberg, Germany
19:50 – 20:50Canteen dinner
Day 9 – Monday 26 August 2024
Time
(Europe/Berlin
ProgrammeLocation
08:40 – 08:45Overview of the day
08:45 – 09:45Lecture: Hot topics: recent breakthroughs and emerging technologies in structural biology via cryoET
Svetlana Dodonova – EMBL Heidelberg, Germany
09:45 – 10:45Lecture: Hot topics: recent breakthroughs and emerging technologies in structural biology via cryoET
Cristina Paulino – Heidelberg University, Germany
10:45 – 11:15Coffee break
11:15 – 12:15Wrap up practicals: highlights presented by participants
12:15 – 13:15Final panel discussion: Wrap up with emphasis on the future of cryoEM, responsible pracitce, reporing and validation standards
13:15 – 13:45Packed lunch and departure

Practical information

Registration fees and motivation letter

The course is limited to up to 24 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.

Registration

Registration fees include admission, course materials, COVID-19 safety measures, meals and coffee breaks.

This EMBO course includes accommodation and transportation to and from the ISG Hotel to the venue.

Academia€500
PhD Student€500
Industry€1000

Confirmation and payment

The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.

Motivation letter submission

After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.

Instructions

  1. Include your relevant skills, experience and qualifications showing why you would be suitable for the course.
  2. Explain why you would like to attend, including what you can contribute and how you think you will benefit from the course.

Please note:

  • The limit of 2000 characters refers to manually typed text and excludes spaces. If an error occurs try using a different web browser (preferably Google Chrome or Mozilla Firefox).
  • If you copy-paste the text into the form, hidden formatting might still be included which may cause the text to exceed the 2,000 character limit resulting in an error message. We recommend you clear all formatting before pasting in the text.
  • If you have special symbols in your text, make sure you are using Unicode characters, otherwise these will not be recognised.

For more detailed information, follow the instructions provided in our video on how to submit a course motivation letter.

Further details

For further information about registration and motivation letter submission please refer to the FAQ page.

Financial assistance

Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of both registration fee waivers and travel grants. Availability is limited to participants attending on-site events in Heidelberg and will be indicated during the abstract or motivation letter submission process.

Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.

Registration fee waiver

The fee waiver will cover the registration sum that you have paid to attend the course or conference.

Travel grant

The travel grant will cover the cost of travel (airfare, train, bus, taxi, accommodation, visa, and/or registration fees*) and is provided up to specified caps which are normally as follows:

– up to €400 for participants travelling to an EMBL Course, EMBL Conference or EMBO|EMBL Symposium from within Europe.
– up to €1000 for participants travelling to an EMBL Course, EMBL Conference or EMBO|EMBL Symposium from outside Europe.
– up to €500 for any participant travelling to an EMBO Practical Course or EMBO Workshop.
– up to €1000 for any participant working in Chile, India, Singapore or Taiwan travelling to an EMBO Practical Course or EMBO Workshop.

*Registration fees are only covered for EMBO Practical Courses or EMBO Workshops

The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.

Childcare grant (EMBO practical courses and workshops only)

For EMBO Practical Course or EMBO Workshop participants with children, there is the possibility to apply for a childcare grant to offset child care costs incurred by participants or speakers when participating at a course or conference. Eligible costs include fees for a baby-sitter or child-care facility, travel costs for a care giver, or travel costs for taking the child to the meeting etc. Please note that priority will be given to early stage researchers. A maximum amount of 500 EUR can be awarded per participant applying for an EMBO Childcare Grant. In order to apply for this grant for EMBO Workshops, you must be registered by the abstract submission deadline.

Application

You may apply for financial assistance when submitting your motivation letter for courses, and abstract for conferences. In your application you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.

For the Boehringer Ingelheim Fonds Travel Grant, there is a pre-application question during the motivation letter submission process, and if selected you will be requested to complete a standard form and documentation consisting of your travel expense estimation.

Selection

The scientific organisers will select the recipients of all financial assistance during the motivation letter or abstract selection process. Results will be announced approximately 6-8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the meeting. Selection is based on your current work or study location, the reasons for needing financial support and the impact this event will have on your career.

Reimbursement

Costs will be reimbursed after the meeting only once a reimbursement form and original receipts (from travel costs) have been received.

Further details

View our list of external funding opportunities and information on attending a conference as an event reporter.

For further information about financial assistance please refer to the FAQ page.

Accommodation and travel

Hotel

Accommodation in twin rooms, including breakfast, is included in the registration fee. Accommodation has been booked for you at the ISG Hotel for the default dates of 18 to 26 (8 nights). In case you would like to have a single room, please let the hotel know and please note that you will be required to pay an additional cost of 24€ per night (availability of single rooms may be limited). This should be paid by you directly to the ISG during your stay. Any additional nights need to be booked and paid for by you. We recommend that all course participants stay at the ISG Hotel which is the dedicated hotel for the course. 

Travel information

For travel information, please see here.

If you are travelling to the conference within Germany then you are eligible for the Deutsche Bahn ‘Event Ticket’ (called the ‘Veranstaltungsticket’ in German). This will result in a lower ticket price if your travel distance to Heidelberg is more than 100 km. You need to provide proof of your event attendance when purchasing the ticket.

For more information in English see here or in German see here.

You can book your ticket here.

Accommodation has been pre-booked for you at the ISG Hotel for the default dates of 18 to 26 August (8 nights). Please note that the course registration fee includes coverage of accommodation costs in twin rooms, including breakfasts, for your stay at the ISG but that you will be required to pay an additional cost of 21 EUR per night for a single room. This should be paid by you directly to the ISG during your stay. Any additional nights need to be booked and paid for by you. We recommend that all course participants stay at the ISG Hotel which is the dedicated hotel for the course.

Local transport

Shuttle buses will go from the ISG Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.

Further details

Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

For more information about accommodation and travel, please refer to the FAQ page.

On-site information

Catering

All meals and coffee breaks are included in the registration fee. Our catering staff will prepare a wide variety of vegetarian meals, meat and fish dishes, soups, pasta, fresh fruit and vegetables, as well as a variety of desserts.

Please wear your badge at all times when serving yourself.

No food or drinks are allowed in the laboratories.

Electricity and charging station

In most places the electricity is 220 volts AC (50 cycles). An adaptor and a plug that fits the German socket may be needed for your appliances/laptop (i.e. American, Japanese, etc.). A USB charging station for electronic devices is available at the registration desk.

EMBL Merchandise

If you are interested in purchasing EMBL merchandise (products presented in the glass display in the registration area), please email the EMBL shop to place an order or get in contact with your Course Organiser.

Kindly note the EMBL shop is only open upon request and all purchases must be made in cash (Euros only).

Health and safety notes

Please read EMBL’s COVID-19 safety policy for on-site events.
Do not smoke in any EMBL building.
Eating and drinking is prohibited in all laboratories.
Do not enter any restricted areas or the laboratories unless instructed to do so.

If first aid is required …

  • The first aid room is located next to the ATC Registration Desk (room 10- 205).
  • Dial the Emergency number 222 from any EMBL internal phone only.
  • Where is the accident? What happened? How many injured?
  • Emergency number 06221-387 7821 from mobile phones.
  • Please report all accidents to the conference officer at the registration desk.

In case of fire …

  • Press the nearest fire alarm button or the Emergency number 222 from any internal phone.
  • A loud fire alarm will go off if an evacuation is required. On hearing the alarm leave the building immediately following the escape route and fire wardens and head to one of the meeting points
  • Do not use the lifts.

Beyond first aid…

Please remember to bring your own medication, if needed, to the conference. Note that the next pharmacy is a 4-minute drive from the EMBL, but for many medications you will be required to see a doctor to get a prescription.

Ensure in advance that your medical insurance will cover you during your visit in the event that you do need to see a doctor while in Heidelberg. In any case, the EMBL Course and Conference Office will assist you to get to the pharmacy and a doctor of your choice if necessary.

Internet

Wi-Fi is available on campus. Log in using the EMBL-Events network and the event specific password, which will be provided on site. The eduroam network (secure, worldwide roaming access service developed for the international research and education community) is also available.

Lost and found

‘’Lost and Found’’ items are kept at the registration desk until the end of the conference.

Luggage

There are lockers available on-site to store your luggage, which require a 2 EURO coin to operate. There is another luggage room on level E0, which is free to use but remains unlocked during the conference.

Nursing room

There is a nursing room available in the ATC Rooftop Lounge on level A29.

Photography

During the conference, an EMBL Photographer may be taking photos. If you would not like to appear in these, please inform the photographer or a member of the Course and Conference Office.

Printing

We can help to print your boarding pass/train ticket. Please send it to events@embl.de and collect your print-outs at the registration desk.

Room for prayer, yoga, and meditation

There is a room for prayer, meditation, and yoga located on level E0 behind the Auditorium. Please be respectful of others using the room.

Sightseeing

A variety of activities in Heidelberg can be found on the website of Heidelberg Marketing.

Travel to and from the venue

During the event, we provide conference shuttle buses to and from EMBL. In addition, there is the public bus 39A that serves the EMBL campus and taxis can be easily booked at any time. Information on the shuttle buses can be found on the individual event website and more detailed information on travelling to EMBL can be found on our Travel Information page.

Additional information

Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

Please find additional information including FAQs, terms and conditions, COVID-19

Technical requirements and event software

Event software

The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus. 

Additional information

Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.

COVID-19 related questions

COVID-19 information for on-site events at EMBL Heidelberg can be found in our COVID-19 FAQs.

Sponsors


Media partners

Imaging and Microscopy, a Wiley magazine



Sponsorship opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other conferences, or contact sponsorship@embl.de for further information.

If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.

Media kit

Want to let others know you’re attending this event? Take a look at our shareable media and feel free to use them in your social media channels or presentations.

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Date: 18 - 26 Aug 2024

Location: EMBL Heidelberg

Venue: EMBL Advanced Training Centre and EMBL Imaging Centre


Deadline(s):

26 May 2024


Organisers:

  • Tanmay Bharat
    MRC Laboratory of Molecular Biology, UK
    • Sebastian Eustermann
      EMBL Heidelberg, Germany
      • Julia Mahamid
        EMBL Heidelberg, Germany

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