Advanced light microscopy support and technology development
Zeiss Lightsheet Z.1
Location: Room 50
System specifications
Dual side illumination
Ultrafast light sheet Pivot scan
4 axis sample positioning system
Sample Chamber holder and overview camera with LED illumination
INDIMO 2971: Preparation for retrofit of optics for cleared samples
CO2-Module Lightsheet Z.1 (D) to incubate the Sample Chamber Lightsheet Z.1 with CO2-enriched air. Includes: TempModule CZ-LSFM with accessories TempModule S1 (D)
XY-stage: motorized
Fully equipped for live imaging, temperature and CO2 controlled
Software: Zen black for image acquisition multiview processing and alignment and deconvolution, Zen blue for measurements and processing
“SWAP” 32TB storage and processing workstation with 96GB RAM – ZEN Software incl. Module “Deconvolution”
Detector
2 detection modules: sCMOS PCOedge
Effective number of pixels: 1000 x 1000
Cell size: 8.0µm x 8.0µm
Effective Area: 8.0mm x 8.0mm
Max frame rate: 28.1 frames/second
Dynamic range: 14 bit
Quantum efficiency (peak): > 60%
Read out noise at min EM gain: 10 electrons
Read out noise at max EM gain: <1 electron
Laserlines
Wavelength (nm)
Laser type and power
Dyes and use
405
solid state laser, 20 mW
DAPI
488
solid state laser, 50 mW
GFP, Alexa488, CY2
561
solid state laser, 20 mW
RedFPs, Alexa568
638
solid state laser, 60 mW
CY5, Alexa647
Band pass Filters
Fluorochromes
Beam Splitter
Filter type
Emission
DAPI / GFP
LP490
Dual
BP 420-70 / BP 505-45
DAPI / RFP
LP510
Dual
BP 420-70 / BP 575-615
GFP / RFP
LP560
Dual
BP 505-45 / BP 575-615
GFP / m-Cherry
LP560
Dual
BP 505-45 / LP 585
GF P/ Far Red
LP560
Dual
BP 505-45 / LP 660
Special optics to generate chromatically corrected light sheets
Illumination Optics 5x/0.1 (in combination with 5X detection objectives)
Illumination Optics 10x/0.2 (in combination with 20X and 63X detection objectives)