Light Imaging Facility

Advanced light microscopy instrumentation and sample preparation services


The following equipment and microscope systems are available at the facility:

  • Stereomicroscope Leica MZ12
  • Motorised fluorescence stereomicroscope Nikon SMZ25
  • Widefield Leica Thunder
  • Laser scanning confocal microscope system Nikon AX/AR
  • XLight V3 Crest Optics Spinning Disk Confocal
  • Structure illumination module Crest Optics DeepSIM
  • Automated slide scanner Olympus VS200
  • Hybrid Open-top Light sheet system Alpenglow
  • Robotic pippetting system Opentrons OT2
  • Automated slide processing system BioGenex NanoVIP
  • GPU-powered Workstation with AIVIA and Imaris for image analysis
  • Cryostat Leica CM3050S
  • Cryostat Thermo Scientific NX70
  • Microtome Leica RM2135 (1 manual and 1 semi-automated)
  • Vibratome Leica VT1000S (2 units)

The following services are available at the facility:

  • Paraffin embedding and serial/step sectioning of tissue or whole embryo samples.
  • Whole-mount in situ hybridisation/immunohistochemistry of early to mid-gestation mouse embryos.
  • Section in situ hybridisation (mRNA, microRNA), immunohistochemistry, and immunofluorescence.
  • Assisting and providing advice to users in the proper extraction of samples for histological analysis.
  • Assisting in characterisation of Cre-lines.
  • In situ sequencing for spatial transcriptomics
  • Expansion microscopy