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Microscopy Facility

Advanced light microscopy support and technology development

Widefield Leica DMR

Location: Room 63

System specifications

  • Upright fluorescence microscope system – Leica DM R
  • Five color excitation filters including blue, CFP and YFP
  • Suitable for fluorophores from DAPI to Texas Red and transmitted image (Brightfield and Phase Contrast).
  • Suitable for fluorophores from DAPI to Texas Red and transmitted image (Brightfield and Phase Contrast).
  • High resolution colored CCD camera CD500
  • Suitable for imaging fixed specimen (no environmental chamber).

Objectives

  • HCX PL FLUOTAR 1.6x/0.05
  • HCX PL FLUOTAR 5x/0.15-PH1
  • HC PL FLUOTAR 10x/0.30-PH1
  • HC PL FLUOTAR 20x/0.50-PH2
  • HCX PL APO 40x/1.25-OIL-PH3
  • HCX PL APO 63x/1.32-OIL-PH3
  • PL APO 100x/1.40 OIL-PH3

Filter cubes

  • 1: A4-UV: Ex filter:BP360/40-Dichromatic mirror:400-Suppression Filter:BP-470/40
  • 2: L5-blue: Ex filter:BP480/40-Dichromatic mirror:505-Suppression Filter:BP-527/30
  • 3: N2.1-green: Ex filter:BP515-560-Dichromatic mirror:580-Suppression Filter:LP590
  • 4: CFP-violet-blue:Exfilter:BP436/20-Dichromatic mirror:455-Suppression Filter:BP480/40
  • 5: YFP- blue:Exfilter:BP500/20-Dichromatic mirror:515-Suppression Filter:BP535/30
  • 6,7,8: EMPTY CUBES

Camera

  • CD500 CCD camera with 1300 x 1030 pixel array
  • 1300X1030 pixels: 4,9,16,36 shots for lower magnification objective

Stage: Manual moving X, Y stage

Contrast enhancement options: Brightfield, Darkfield and phase contrast

Power up and down procedure

  • Please contact Alvaro Crevenna to schedule a session
  • Report problems on the Log file

Book this resource online (intranet)

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