Advanced light microscopy support and technology development
CREST Optics X-light V3 Spinning disk system, Lumencor CELESTA 7-line laser engine, NIKON Ti microscope body, controlled by NIKON NIS
Location: Room 53
Spinning disk confocal on an inverted microscope with a 95% quantum efficiency sCMOS camera allowing high-speed confocal imaging that is also well suited for live-cell imaging and high-resolution morphological imaging.
350-900 nm spectral detection range with 5 nm spectral resolution and sub-micron spatial resolution
Multi-dimension capability: xyz, xzy, xt, xyt, xzt, xyzt, xy, xz, xyt, xyz
Pinhole size: 50 µm
Software: NIS AR, Version 1.7.0, for image acquisition and processing. Includes NIS denoise.ai and JOBS for building complex acquisition pipelines.
Data structure: 16-bit images (*.tif) as default, also possible 12 bit.
Image size: 2048 x 2048 pixels
Photometrics Prime BSI, 95% quantum efficiency sCMOS
Type | Wavelenght (nm) | Dyes and use |
---|---|---|
UV | 405 | DAPI, Hoechst |
Argon | 458, 476, 488, 496, 514 | CyanFP, GFP, Alexa488, CY2 |
561 | RedFPs, Alexa568 | |
594 | Alexa 594, mCherry | |
HeNe | 633 | Cy5, Alexa 647 |
Leica DMI 6000 CS AFC Bino inverted microscope with motorized XY-stage
Magnification | NA | Type | Immersion | Coverslip |
---|---|---|---|---|
20x * | 0.70 | PL Apo UV | Imm/Corr | – / 0.17 |
40x | 1.25 | PL Apo UV | Oil | 0.17 |
63x | 1.20 | PL Apo UV | Water | 0.14 – 0.18 |
63x | 1.4 | PL Apo UV | Oil | 0.17 |
* The 20x immersion objective for water, glycerine and immersion oil
Leica EL6000 External light source for fluorescence excitation (alignment-free, mercury metal halide bulb with an extremely long lifetime of more than 3000 hours)
Name | Excitation filter | Emission filter | Dichroic |
---|---|---|---|
A | BP 340 – 380 nm | LP 430 nm | 400 nm |
CFP | BP 436/20 nm | BP 480/40 nm | 455 nm |
GFP | BP 470 40 | BP 525/50 nm | 580 nm |
N2.1 | BP 515 – 560 nm | LP 590 nm | 580 nm |
YFP | BP 500/20 nm | BP 535/30 nm | 515 nm |
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