P13 parameters

• Beam: P13 offers beam with diameters between 15 and 100 micrometers and divergence below 0.15 mrad. Typical crystal lifetimes are <3 min.
• Energy tunability: The beamline is tunable between 6.5 and 17.5 keV (1.9 Å to 0.7 Å). Fluorescence spectra can be recorded with an AmpTek silicon drift detector.
• Diffractometer: The diffractometer is an MD2 instrument including a mini-kappa goniostat. The horizontal spindle-axis has a sphere of confusion of less than 2 micron when the mini-kappa goniostat is mounted. Three-click centering and 4D-scans are available.
• Detector: EIGER 16M (133 Hz, 750 Hz in 4M ROI mode).
• User Interface: The user interacts with the beamline via the Hamburg version of mxCuBE v2 user interface.
• Sample mounting: Crystals can be mounted manually. A MARVIN robot for mounting SPINE pins from a large capacity storage dewar (170 samples) is available; cycle time between two crystals is < 1 min. Only EMBL-SPINE pucks are supported!
• Data processing: 1144 core cluster is available for online data processing with XDS, EDNA fast processing and autoPROC. DIALS and XIA2 are installed.
• Data storage and archiving: Diffraction images are stored on a fast petabyte cluster storage system in gzip-compressed mini-cbf format. Instructions on how to download your data can be found on the data backup page.

Typical experiments presently possible

• Collection of native data on crystals with minimum dimension of 20 micron.
• Maximum resolution achievable: 1.1 Å at detector edge using 17 keV radiation.
• Collection of highly redundant data using different kappa settings.
• Crystal characterization with beam sizes between 15 and 100 micron.
• Typical duration of a full data collection: 30 sec.
• Full MAD-phasing capability.

How to reference P13

The use of the EMBL beamlines must be acknowledged in all publications or presentations arising from data collection at the EMBL Hamburg unit. The following note is expected to appear in the acknowledgements, giving reference to the station(s) used, supporting beamline staff or any other assistance or information as appropriate, e.g. EU contract numbers, proposal numbers.

The synchrotron data was collected at beamline operated by EMBL Hamburg at the PETRA III storage ring (DESY, Hamburg, Germany). We would like to thank N.N.** for the assistance in using the beamline.

**If appropriate, names of the beamline staff in case they are not co-authors.In case of P13, please cite the beamline paper:

Cianci, Bourenkov, Pompidor et al. J.Synchr.Rad. 24:323-332 (OPEN ACCESS)