Protein quality control for downstream processes – Course and Conference Office

EMBL Course

Protein quality control for downstream processes


Proof of COVID-19 vaccination or recovery is required to attend this on-site course. Please see EMBL’s COVID-19 safety policy for on-site events.

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Course Overview

Purified proteins are used in many biological experiments, including for structure determination, studying protein interactions, identifying drug metabolism and specificity, mechanistic studies, in vivo experiments and many other applications. In order for these experiments to be biologically relevant, they must be performed using high quality protein that is correctly folded, active, contains properly inserted cofactors and is in the correct oligomeric state. Ensuring that these parameters are fulfilled requires protein quality control. In this course, we will review some quality control basics and their application along with a description of various techniques that may be used to ensure high quality samples to produce reliable and reproducible data in downstream processes.


This course is aimed at life science researchers of all levels who would like to learn how to assess the quality of their purified proteins. Prior experience with protein quality control methods is not necessary, but it might be helpful to have a basic understanding of protein expression and purification. The course will focus on introducing the importance of protein quality control and how to approach and apply various techniques for ensuring high quality samples that will produce reliable data in downstream applications.


  • Thermal shift assays: NanoDSF and Thermofluor for buffer screening
  • Interaction studies: ITC and MST
  • Light Scattering: SEC-MALS and DLS
  • Cryo-EM: how sample quality control can make a difference in resolution

Learning Outcomes

After this course you should be able to:

  • Assess the quality of your protein(s) and protein complexes using the methods presented in the course
  • Analyse and interpret the data obtained from different biochemical and biophysical methods commonly used in protein quality control
  • Know which methods and technologies you can apply to try to improve the quality of your protein(s) and protein complexes
  • Decide which quality control parameters are the most important ones for different types of downstream applications

Scientific Organisers

Course Organisers

Yvonne Yeboah

Yvonne Yeboah

Training Laboratory Manager

EMBL Heidelberg



Sponsorship Opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other events, or contact sponsorship@embl.de for further information.

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Date: 28 Nov - 2 Dec 2022

Location: EMBL Heidelberg

Venue: EMBL Advanced Training Centre


EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme

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