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Regulation of translation is key in the control of gene expression, playing a critical role in many biological and pathophysiological settings. In the last decade, methodological advances have provided comprehensive insights into cellular transcriptomes and proteomes, however, the systems-wide analysis of translation remained an unsolved experimental challenge. The advent of ‘ribosome-profiling’ provided the methodological basis for comprehensive transcriptome-wide analyses of translation at sub-codon resolution by the precise and high-throughput determination of ribosomal positions on mRNA. Its application in a variety of organisms yielded novel insights into protein synthesis and its dynamic regulation, significantly advancing our understanding of the post-transcriptional regulation of gene expression.
The practical course ‘Measuring Translational Dynamics by Ribosome Profiling’ aims to:
a) generate a detailed theoretical understanding of ribosome-profiling, its variations to address specific aspects of translation and its experimental strengths and limitations,
b) provide hands-on wet-lab training to perform ribosome-profiling experiments with the aim to understand and master the critical steps of the experimental workflow and to train the participants to independently perform and adapt similar experiments in their own laboratories,
c) provide hands-on bioinformatics training on processing the high-throughput sequencing data, the quality control and on the use of specialised tools for data generated by ribosome-profiling.
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