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FISHing for RNAs: classical to single molecule approaches – Course and Conference Office

EMBO Practical Course

FISHing for RNAs: classical to single molecule approaches

Overview
Speakers
Sponsors
Media kit

Please see EMBL’s COVID-19 safety policy for testing and other requirements to attend this on-site course.

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Course overview

Over the past decades it became evident that a RNA transcripts specific amount and localisation are important in order to understand the biological function of its (non-) coding gene. Whereby state-of-the-art in situ hybridisation (ISH) technologies are key to visualise these RNAs in a given tissue or cell.

This course aims to highlight three powerful RNA visualisation techniques in histological tissue samples. During this course, the participants will learn to validate the cellular localisation of candidate genes from transcriptome studies through conventional ISH and, the more recent cutting-edge, smFISH (Single Molecule Fluorescent ISH) and HCR-ISH (Hybridization Chain Reaction), based RNA ISH hybridisation methods. Additionally, the course will also cover essential tissue embedding protocols that are adapted for use in visually interrogating RNA transcripts in mammalian tissues.

Emerald Perlas

EMBL Rome

Italy

Tiziana Santini

University of Rome, La Sapienza

Italy

Tim Weil

University of Cambridge

Scientific organisers

Monica Ballarino

University of Rome, La Sapienza

Italy

Imre Gaspar

Turbine.ai

Hungary

Emerald Perlas

EMBL Rome

Italy

Elisabeth Zielonka

EMBL Heidelberg

Germany

Course organisers

Charlotte Courtney

Course and Conference Officer

EMBL Heidelberg

Germany

Swetlana Krez

Training Lab Technician

EMBL Heidelberg

Germany

Sponsors

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Media kit

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