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FISHing for RNAs: classical to single molecule approaches – Course and Conference Office

EMBO Practical Course

FISHing for RNAs: classical to single molecule approaches

Overview

Course overview

Over the past decades, it became evident that the specific amount and localisation of an RNA transcript are important to understand the biological function of its (non-) coding gene. Whereby state-of-the-art in situ hybridisation (ISH) technologies are key to visualise these RNAs in a given tissue or cell.

This course will teach participants to apply three powerful RNA visualisation techniques, all in histological tissue samples: conventional in situ hybridisation (ISH), hybridisation chain reaction ISH (HCR-ISH), and the cutting edge single molecule fluorescent ISH (smFISH). In addition, essential tissues embedding protocols adapted to facilitate visual interrogation of RNA transcripts will also be covered.

Benefit from our excellent trainer to participant ratio for personalised training in addition to world class research facilities and networking opportunities with leading experts in the field.

Audience

This course is targeted to early stage scientists (PhD and postdocs) across the field of molecular biology with an interest in application of these techniques for their research.

Onboarding day

This hybrid course will begin on 6 October 2023, with a joint onboarding session for on-site and virtual participants – where access to pre-course study materials, exercises and pre-recorded lectures will be provided through an introduction to our collaboration platform EMBL eCampus. This will prepare the participants for the course start on 22 October 2023.

Full-time attendance is required for both virtual and on-site components of this course. 

Information on the live stream and access to the virtual course platform will be provided shortly before the start of the event and will be available for 2 weeks after the course.

Lectures

All lectures will be delivered on site and live-streamed to all virtual participants via Zoom. Please see the preliminary programme for details on all lectures.

On-site practicals

These are the practicals for the course, all of the content will be delivered on-site in the training laboratories and the microscopy rooms at EMBL. The practicals will not be live-streamed to virtual participants, please see below for the virtual practical information which will take place in tandem to the on-site practicals:

  • Practical session 1: Introduction and theory of conventional ISH
  • Practical session 2: Probe preparation (ddUTP + dye conjugation)
  • Practical 3a and 3b: Introduction and theory smFISH and HCR
  • Practical 4: Imaging in groups (smFISH and HCR-ISH)
  • Practical session 5: Basics ISH image analysis

Virtual practicals

The content of the virtual practicals will be delivered in the form of interactive protocols as well as video tutorials recorded by the trainers. The following content will be available for virtual participants during the above on-site practical sessions. Please see the preliminary programme for when these will take place:

  • Overview of the complete workflow
  • smFISH probe design (~40 min video-Material)
  • HCR split initiator design (~45 min video-Material)
  • Dye conjugation of amino-11-ddUTP (12 min video material)
  • Probe labelling for smFISH (~30 min video-Material)
  • smFISH and HCR (30 min video material)
  • Image analysis (~60 min video-material)

The interactive protocol will cover NcRNA or microRNA in situ hybridisation on paraffin sections of mouse tissues.

We will also include Q&A sessions with trainers to give participants the opportunity to ask questions about the procedures of the material given.

.

Please see EMBL’s COVID-19 safety policy for testing and other requirements to attend this on-site course.

Speakers

Speakers, trainers, and staff

Chiara Capponi

University of Rome, La Sapienza

Italy

Jeffrey A. Chao

Friedrich Miescher Institute for Biomedical Research

Switzerland

Eleonora D’Ambra

Center for Life Nano- & Neuro-Science of Istituto Italiano di Tecnologia (IIT)

Italy

Scientific organisers

Course organisers

Charlotte Courtney

Course and Conference Officer

EMBL Heidelberg

Germany

Programme

Please note – This programme is preliminary at the moment and is subject to change.

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  • Some pre-course work (such as watching pre-recordings, protocol reading and computational exercises) with estimated time 0-10 hours will be required and will be shared in advance of the course.
  • Some speakers may need to join virtually to give their lecture or Q&A, and the programme is subject to change.
Day 0 – Friday 6 October 2023
Time (Europe/Berlin)Programme
Milestones of the dayMilestones of the day:
-How to use eCampus
-Explain milestones
-How to follow the workflow
-How to use the protocol
-How to use the resources
-VMs
-Watch pre-recorded lectures by Sunday 22nd October 2023 – the start of the course (eCampus, lectures 1, 2, 3 )
13:00 – 13:20Welcome – virtual onboarding
13:20 – 13:40Practical parts – Online Protocols
13:40 – 14:40Ice breaker
14:40 – 15:00Wrap up
Day 1 – Sunday 22 October 2023
Time (Europe/Berlin)Onsite ProgrammeVirtual Programme
Milestones of the dayMilestones of the day:
None
13:45 – 14:00Bus from ISG hotel to EMBLLog on and check connection
14:00 – 14:30Welcome and house notes and course overviewWelcome and house notes and course overview
14:30 – 16:30Ice breaking activity – Flash talks and activity in groups



with concurrent coffee break
Ice breaking activity – Flash talks and activity in groups

16:30 – 17:15Keynote Lecture 1: Applying RNA imaging methodologies to tackle ncRNAs in brain 
Marie-Laure Baudet – University of Trento, Italy
Keynote Lecture 1: Applying RNA imaging methodologies to tackle ncRNAs in brain 
Marie-Laure Baudet – University of Trento, Italy
17:15 – 18:00Keynote Lecture 2: Transcriptional profiling of spermatogenesis in human and issues in infertility.
Sara Di Persio – Münster University Hospital, Germany
Keynote Lecture 2: Transcriptional profiling of spermatogenesis in human and issues in infertility.
Sara Di Persio – Münster University Hospital, Germany
18: 00 – 18:45Comfort breakComfort break
18:00 – 21:00Moderated discussion rounds with concurrent dinner in the canteenModerated discussion rounds – The onsite participants will also eat during the time, please feel free to do this too.
18:45 – 19:30Discussion to Prerecorded LECTURE 1 – Topic: Histology overview and applications
Moderated by: Emerald Perlas – EMBL Rome, Italy
Discussion to Prerecorded LECTURE 1 – Topic: Histology overview and applications
Moderated by: Emerald Perlas – EMBL Rome, Italy
19:30 – 20:15Discussion to Prerecorded LECTURE 2 – Topic: RNA detection methods
Moderated by: Imre Gaspar – Turbine.ai, Hungary
Discussion to Prerecorded LECTURE 2 – Topic: RNA detection methods
Moderated by: Imre Gaspar – Turbine.ai, Hungary
20:15 – 21:00Discussion to Prerecorded LECTURE 3 – Topic:ncRNA Charme in cardiac and skeletal muscle
Moderated by: Monica Ballarino, University of Rome, La Sapienza, Italy
Discussion to Prerecorded LECTURE 3 – Topic:ncRNA Charme in cardiac and skeletal muscle
Moderated by: Monica Ballarino, University of Rome, La Sapienza, Italy
21:00 – 21:15Wrap upWrap up
21:15Departure to Hotel
Day 2 – Monday 23 October 2023
Time (Europe/Berlin)Onsite ProgrammeOnsite locationVirtual Programme
Milestones of the dayMilestones of the day:
Practical 1: Introduction and Preparation for practical e.g. buffer prep
Practical 1: Protocol steps 1-5
Practical 2: Protocol and Video: Dye conjugation of amino-11-ddUTP (12 min)
08:30 – 09:00Arrival and coffee
09:00 – 09:10Overview of the dayFlex labsOverview of the day
09:10 – 09:30Lab safety instructions  Flex labsLab safety instructions  
9:30 – 10:45Interactive lecture/demo: Specimen section preparation

Emerald Perlas – EMBL Rome, Italy
Training Lab A & BLIVESTREAM PRACTICAL: Interactive lecture/demo: Specimen section preparation
Emerald Perlas – EMBL Rome, Italy
10:45 – 11:00Coffee breakFlex labsEmerald continues and gives intro to conventional ISH for virtual participants, Imre gives an overview too
11:00 – 11:45Keynote Lecture 7: Neuronal sorting of RNA.
Marina Chekulaeva – Max Delbrück Center for Molecular Medicine, Germany
Flex labsKeynote Lecture 7: Neuronal sorting of RNA.
Marina Chekulaeva – Max Delbrück Center for Molecular Medicine, Germany
11:45 – 12:30Keynote Lecture 4: Interplay between genetic and environmental factors in shaping body plans.
Aissam Ikmi – EMBL Heidelberg, Germany
Flex labsKeynote Lecture 4: Interplay between genetic and environmental factors in shaping body plans.
Aissam Ikmi – EMBL Heidelberg, Germany
12:30 – 13:30Lunch breakEMBL Canteen
13:30 – 14:15Practical session 1: Introduction and theory of conventional ISH. Protocol Overview.
Emerald Perlas assisted by Chiara Capponi, Sara Di Persio, and Tiziana Santini
Training Lab A & B
14:15 – 14:45Practical session 2: Probe preparation (ddUTP + dye conjugation)
Imre Gaspar assisted by 3 helpers
Training Lab A & B
14:45 – 16:45Practical session 1: Conventional ISH; (section pre-treatment, Proteinase K digest, acetylation, hybridization set-up).
Emerald Perlas assisted by Chiara Capponi, Sara Di Persio, and Tiziana Santini
Training Lab A & B
16:45 – 17:00Coffee breakFlex labs
17:00 – 17:45Keynote Lecture 5: Phase separation in RNA localization.
Mainak Bose – EMBL Heidelberg, Germany
Flex labsKeynote Lecture 5: Phase separation in RNA localization.
Mainak Bose – EMBL Heidelberg, Germany
17:45 – 18:00Comfort break
18:00 – 19:30DinnerEMBL Canteen
19:30 – 21:30Student presentations: 10 student presentations (each: 5 min presentation plus 6 min discussion)

Flex labsStudent presentations: 10 student presentations (each: 5 min presentation plus 6 min discussion)
21:40Departure to HotelATC Entrance
Day 3 – Tuesday 24 October 2023
Time (Europe/Berlin)Onsite ProgrammeVirtual Programme
Milestones of the dayMilestones of the day:
Practical 3a and 3b:

-Video: smFISH probe design (part1, part 2, part3) (36 min)
-Video: HCR design (part1,part2) (43 min)
-OPTIONAL: Preparation of buffers for lab
-Protocol steps ‘probe labelling for smFISH’
-Video: probe labelling smFsFISH (part 1, part 2, part 3) (22 min)
-Video: sm FISH and HCR-ISH specimen pre-work and hybridisation set-up (8 min)
Practical 1: Protocol steps 6-end
08:30 – 09:00Arrival and coffee
09:00 – 09:10Overview of the day
09:10 – 10:15Practical session 1: (conventional ISH) post-hybridisation washes
Emerald Perlas assisted by Chiara Capponi, Sara Di Persio, and Tiziana Santini
10:15 – 11:00Keynote Lecture 3: RNP granule dynamics
Florence Besse – University of Nice, Institut de Biologie Valrose (iBV), France
Keynote Lecture 3: RNP granule dynamics
Florence Besse – University of Nice, Institut de Biologie Valrose (iBV), France
11:00 – 11:15Coffee break
11:15 – 12:00Practical session 1: Conventional ISH: staining set-up
Emerald Perlas assisted by Chiara Capponi, Sara Di Persio, and Tiziana Santini
12:00 – 13:00Lunch at EMBL canteen
13:00 – 13:30Practical session 2: Theory and demo of probe design
Imre Gaspar assisted by 3 helpers
LIVESTREAM PRACTICAL – Practical session 2: Theory and demo of probe design
Imre Gaspar assisted by 3 helpers
13:30 – 14:30Practical 3a and 3b: Introduction and theory smFISH and HCR
Monica Ballarino, Imre Gaspar, Eleonora D’Ambra, Tiziana Santini
LIVESTREAM PRACTICAL – Practical 3a and 3b: Introduction and theory smFISH and HCR
Monica Ballarino, Imre Gaspar, Eleonora D’Ambra, Tiziana Santini
14:30 – 15:00Coffee break
15:00 – 16:00Practical session 1: Conventional ISH: washing and mounting of specimen
Emerald Perlas assisted by Chiara Capponi, Sara Di Persio, and Tiziana Santini
16:00 – 16:30Practical 3a and 3b: sm FISH and HCR-ISH specimen pre-work and hybridisation set-up
Monica Ballarino, Imre Gaspar, Eleonora D’Ambra, Tiziana Santini
16:30 – 17:00Practical 3a: sm FISH: Probe labeling set-up
Monica Ballarino, Imre Gaspar, Eleonora D’Ambra, Tiziana Santini
ON incubation sm labeling!
17:00 – 18:00Practical 3a and 3b: continued: HCR-ISH specimen pre-work and hybridisation set-up
Monica Ballarino, Imre Gaspar, Eleonora D’Ambra, Tiziana Santini
18:00 – 19:00Dinner at EMBL
19:00 – 21:1011 student presentation (each: 5 min presentation plus 6 min discussion)


11 student presentation (each: 5 min presentation plus 6 min discussion)
21:15Departure to Hotel
Day 4 – Wednesday 25 October 2023
Time (Europe/Berlin)Onsite ProgrammeVirtual Programme
MilestonesMilestones of the day:
Practical 3a and 3b:

-Protocol parts: ‘smFISH in myotubes and ‘HCR’
-Video: smFISH and HCR generics (part 1, part2) (23 min)
-Video: Mounting?
08:30 – 09:00Arrival and coffee
09:00 – 09:10Overview of the day
09:10 – 11:30Practical 3a and 3b:
smFISH: Probe purifications and measurements
HCR-ISH: washes
smFISH pre-hybridisation set-up for incubation
HCR-ISH: Amplification set-up for reaction
smFISH hybridisation
Monica Ballarino, Imre Gaspar, Eleonora D’Ambra, Tiziana Santini
With concurrent coffee break
11:30 – 12:15Keynote Lecture 8: Adaptable P body physical states differentially regulate bicoid mRNA storage during early Drosophila development
Tim Weil – University of Cambridge, UK
Keynote Lecture 8: Adaptable P body physical states differentially regulate bicoid mRNA storage during early Drosophila development
Tim Weil – University of Cambridge, UK
12:15 – 13:15Lunch break at EMBL canteen
13:15 – 15:45Practical session 1: Conventional ISH imaging
Emerald Perlas assisted by Chiara Capponi, Sara Di Persio, and Tiziana Santini
With concurrent coffee break
LIVESTREAM Practical with Emerald and Trainers
15:45 – 16:30Keynote Lecture 9: Membrane associated RNA transport
Michael Feldbruegge – Institute of Microbiology Heinrich Heine University, Germany
Keynote Lecture 9: Membrane associated RNA transport
Michael Feldbruegge – Institute of Microbiology Heinrich Heine University, Germany
16:30 – 18:00Practical 3a and 3b: smFISH & HCR-ISH: washes and specimen mounting
Monica Ballarino, Imre Gaspar, Eleonora D’Ambra, Tiziana Santini
With concurrent coffee break
18:00 – 19:00Dinner at EMBL
19:00 – 20:30DISCUSSION ROUND (responsible conduct of research)
Experimental design
Standards and controls in the ISH field
DISCUSSION ROUND (responsible conduct of research)
Experimental design
Standards and controls in the ISH field
20:40Departure to Hotel
Day 5 – Thursday 26 October 2023
Time (Europe/Berlin)Onsite ProgrammeVirtual Programme
Milestones of the dayMilestones of the day:
Prepare for discussion rounds with Emerald, Monica and co.
08:30 – 09:00Arrival and coffee
09:00 – 09:10Overview of the day
09:10 – 10:30Practical 4: Imaging in groups ( smFISH and HCR-ISH)
Monica Ballarino, Imre Gaspar, Eleonora D’Ambra, Tiziana Santini
10:30 – 10:45Coffee break and group photoGroup photo – Please take a screen shot of yourselves on Zoom, we will add this to the group photo.
10:45 – 12:15Practical 4: Imaging in groups ( smFISH and HCR-ISH) Monica Ballarino, Imre Gaspar, Eleonora D’Ambra, Tiziana SantiniMeet the speaker for the virtual participants
12:15 – 13:15Lunch at EMBL canteen, table set-up based on next career steps
13:15 – 14:00Keynote Lecture 10: RNP assembly for transport and translational control
Elizabeth Gavis – Princeton University, USA
Keynote Lecture 10: RNP assembly for transport and translational control
Elizabeth Gavis – Princeton University, USA
14:00 – 14:45Keynote Lecture 6: Dynamics of localized translation of mRNA
Jeffrey Chao – Friedrich Miescher Institute for Biomedical Research, Switzerland
Keynote Lecture 6: Dynamics of localized translation of mRNA
Jeffrey Chao – Friedrich Miescher Institute for Biomedical Research, Switzerland
14:45 – 16:15Meet the speakers for the on-site participantsLIVESTREAM – Practical 4: Imaging ( smFISH and HCR-ISH) with Imre Gaspar
16:15 – 16:30Coffee breakCoffee break
16:30 – 17:15Keynote Lecture 11: PAINT superresolution imaging of nucleic acids
Ralf Jungmann – Max Planck Institute of Biochemistry, Germany
Keynote Lecture 11: PAINT superresolution imaging of nucleic acids
Ralf Jungmann – Max Planck Institute of Biochemistry, Germany
17:30 – 18:00Bus to Heidelberg
18:00 – 19:30Heidelberg Guided City tour
19:30 -22:30Dinner at Kulturbrauerei in downtown Heidelberg
Day 6 – Friday 27 October 2023
Time (Europe/Berlin)Onsite ProgrammeVirtual Programme
Milestones of the dayMilestones of the day:
None
08:30 – 09:00
09:00 – 10:00Discussion on Practical 4: Summary of Image taking, including best practices and standards
Monica Ballarino, Imre Gaspar, Eleonora D’Ambra, Tiziana Santini
10:00 – 10:30Coffee break
10:30 – 11:50Practical session 5: Basics ISH image analysis
Imre Gaspar assisted by 3 helpers
LIVESTREAM – Practical 5: Basics ISH image analysis
with Imre Gaspar
11:50 – 13:20Practical session 5: ISH image analysis (incl. standards in the field)
Imre Gaspar assisted by 3 helpers
LIVESTREAM – Practical 5: ISH image analysis
with Imre Gaspar
13:20 – 14:20Lunch at EMBL canteen
14:20 – 15:20Group work: Participants preparing group presentations
supported by trainers with concurrent coffee break
Virtual participants welcome to join, but no virtual presentation possible
15:20 – 16:20Discussion: Group Data Presentations incl. Discussions with concurrent coffee breakDiscussion: Group Data Presentations incl. Discussions
16:20 – 17:20Wrap upWrap up
17:45Bus to Heidelberg train station and city center
Post course day – Friday 3rd November 2023
Time (Europe/Berlin)Onsite ProgrammeVirtual Programme
13:00-15:00Postcourse day: Virtual session – Open discussion round on questions to practicals (first questions will be collected via EMBL eCampus)

All main-trainers
Postcourse day: Virtual session – Open discussion round on questions to practicals (first questions will be collected via EMBL eCampus)

All main-trainers

Practical information

Registration fees and motivation letter

The course is limited to 16 onsite participants and 5 virtual. For selection purposes, please note that your application will not be considered without a letter of motivation.

Registration

On-site registration fees include admission, course materials, meals and coffee breaks. This EMBO course includes accommodation and transportation to and from the ISG Hotel to the venue.

Virtual registration fees include access to live-streamed course content (lectures, Q&A sessions with speakers and trainers, discussion rounds) as well as virtual learning activities in the learning platform.

On-site Academia€500
On-site PhD Student€500
On-site Industry€1200
Virtual Academia€190
Virtual PhD Student€190
Virtual Industry€250

A letter to support your visa application will be issued, on request, once payment of the registration fee is confirmed. We recommend that you book your visa appointment as soon as possible, to avoid any delay with your visa application.

Confirmation and payment

The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.

Motivation letter submission

After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively, you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.

Instructions

  1. Include your relevant skills, experience and qualifications showing why you would be suitable for the course.
  2. Explain why you would like to attend, including what you can contribute and how you think you will benefit from the course.

Please note:

  • The limit of 2000 characters refers to manually typed text and excludes spaces. If an error occurs try using a different web browser (preferably Google Chrome or Mozilla Firefox).
  • If you copy-paste the text into the form, hidden formatting might still be included which may cause the text to exceed the 2,000 character limit resulting in an error message. We recommend you clear all formatting before pasting in the text.
  • If you have special symbols in your text, make sure you are using Unicode characters, otherwise these will not be recognised.

For detailed instructions, please watch our video on how to submit a course motivation letter.

Further details

For further information about registration and motivation letter submission please refer to the FAQ page.

Financial assistance

Financial assistance

Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of both registration fee waivers and travel grants. Availability is limited to participants attending on-site EMBL Courses and virtual or on-site EMBO Practical Courses in Heidelberg, and will be indicated during the motivation letter submission process.

Your place in the course is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.

Registration fee waiver

The fee waiver will cover the registration sum that you have paid to attend the course.

Travel grant

The travel grant will cover the cost of travel (airfare, train, bus, taxi, accommodation, visa, and/or registration fees*) and is provided up to specified caps which are normally as follows:

– up to €400 for participants travelling to an EMBL Course within Europe.

– up to €1000 for participants travelling to an EMBL Course from outside Europe.

– up to €500 for any participant travelling to an EMBO Practical Course.

– up to €1000 for any participant working in Chile, India, Singapore or Taiwan travelling to an EMBO Practical Course.

– up to €700 for any participant working in Croatia, Czech Republic, Estonia, Greece, Hungary, Italy, Lithuania, Luxembourg, Poland, Slovenia, and Turkey travelling to an EMBO Practical Course.

*Registration fees are only covered for EMBO Practical Courses

The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.

Childcare grant (EMBO Practical Courses only)

For EMBO Practical Course participants with children, there is the possibility to apply for a childcare grant to offset childcare costs incurred by participants or speakers when participating at a course. Eligible costs include fees for a babysitter or child-care facility, travel costs for a caregiver, or travel costs for taking the child to the meeting etc. Please note that priority will be given to early-stage researchers. A maximum amount of €500 can be awarded per participant applying for an EMBO childcare grant. 

Boehringer Ingelheim Fonds travel grant

Applies to selected courses only. Availability will be indicated during the motivation letter submission process.

This grant covers costs related to your attendance at the course (registration, travel and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.

Whether you are eligible to apply for a travel grant, depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):

– for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course

– for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course

Application

You may apply for financial assistance when submitting your motivation letter for courses. In your application, you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.

For the Boehringer Ingelheim Fonds Travel Grant, there is a pre-application question during the motivation letter submission process, and if selected you will be requested to complete a standard form and documentation consisting of your travel expense estimation.

Selection

The scientific organisers will select the recipients of all financial assistance during the motivation letter selection process. Results will be announced approximately 6-8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the course. Selection is based on scientific merit, your current work or study location, the reasons for needing financial support, and the impact this event will have on your career.

Reimbursement

Costs will be reimbursed after the meeting only once a reimbursement form and original receipts (from travel costs) have been received.

Further details

See our list of external funding opportunities and for further information about financial assistance please refer to the FAQ page.

Accommodation and shuttles

Hotel

Accommodation has been pre-booked for you at the ISG Hotel for the default dates of Sunday 22 October to Friday 27 October 2023 (5 nights).

Please note that the course registration fee includes coverage of accommodation costs in twin rooms, including breakfasts, for your stay at the ISG. In case you would like to have a single room, please let us know and please note that you will be required to pay an additional cost of 24€ per night. This should be paid by you directly to the ISG during your stay. Any additional nights need to be booked and paid for by you.

We recommend that all course participants stay at the ISG Hotel which is the dedicated hotel for the course.

Local transport

Shuttle buses will go from the ISG Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.

Further details

Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

For further information on getting to EMBL Heidelberg visit our Travel Information page.

For enquiries about accommodation and local transportation please refer to the FAQ page.

On-site information

Catering

All meals and coffee breaks are included in the registration fee. Our catering staff will prepare a wide variety of vegetarian meals, meat and fish dishes, soups, pasta, fresh fruit and vegetables, as well as a variety of desserts.

Please wear your badge at all times when serving yourself.

No food or drinks are allowed in the laboratories.

Electricity and charging station

In most places the electricity is 220 volts AC (50 cycles). An adaptor and a plug that fits the German socket may be needed for your appliances/laptop (i.e. American, Japanese, etc.). A USB charging station for electronic devices is available at the registration desk.

EMBL Merchandise

If you are interested in purchasing an EMBL souvenir (products presented in the glass display in the registration area), please ask at the registration desk for more information.

Health and safety notes

Please read EMBL’s COVID-19 safety policy for on-site events.
Do not smoke in any EMBL building.
Eating and drinking is prohibited in all laboratories.
Do not enter any restricted areas or the laboratories unless instructed to do so.

If first aid is required …

  • The first aid room is located next to the ATC Registration Desk (room 10- 205).
  • Dial the Emergency number 222 from any EMBL internal phone only.
  • Where is the accident? What happened? How many injured?
  • Emergency number 06221-387 7821 from mobile phones.
  • Please report all accidents to the conference officer at the registration desk.

In case of fire …

  • Press the nearest fire alarm button or the Emergency number 222 from any internal phone.
  • A loud fire alarm will go off if an evacuation is required. On hearing the alarm leave the building immediately following the escape route and fire wardens and head to one of the meeting points
  • Do not use the lifts.

Beyond first aid…

Please remember to bring your own medication, if needed, to the conference. Note that the next pharmacy is a 4-minute drive from the EMBL, but for many medications you will be required to see a doctor to get a prescription.

Ensure in advance that your medical insurance will cover you during your visit in the event that you do need to see a doctor while in Heidelberg. In any case, the EMBL Course and Conference Office will assist you to get to the pharmacy and a doctor of your choice if necessary.

Internet

Wi-Fi is available on campus. Log in using the EMBL-Events network and the event specific password, which will be provided on site. The eduroam network (secure, worldwide roaming access service developed for the international research and education community) is also available.

Lost and found

‘’Lost and Found’’ items are kept at the registration desk until the end of the conference.

Luggage

There are lockers available on-site to store your luggage, which require a 2 EURO coin to operate. There is another luggage room on level E0, which is free to use but remains unlocked during the conference.

Nursing room

There is a nursing room available in the ATC Rooftop Lounge on level A29.

Photography

During the conference, an EMBL Photographer may be taking photos. If you would not like to appear in these, please inform the photographer or a member of the Course and Conference Office.

Printing

We can help to print your boarding pass/train ticket. Please send it to events@embl.de and collect your print-outs at the registration desk.

Room for prayer, yoga, and meditation

There is a room for prayer, meditation, and yoga located on level E0 behind the Auditorium. Please be respectful of others using the room.

Sightseeing

A variety of activities in Heidelberg can be found on the website of Heidelberg Marketing.

Travel to and from the venue

During the event, we provide conference shuttle buses to and from EMBL. In addition, there is the public bus 39A that serves the EMBL campus and taxis can be easily booked at any time. Information on the shuttle buses can be found on the individual event website and more detailed information on travelling to EMBL can be found on our Travel Information page.

Additional information

Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.

Technical requirements and event software

Event software

The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus. 

Additional information

Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.

COVID-19 related questions

COVID-19 information for on-site events at EMBL Heidelberg can be found in our COVID-19 FAQs.

Sponsors

Event partner:

Event sponsor:

Event supporter:

Sponsorship opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other events, or contact sponsorship@embl.de for further information.

If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.

Warning

EMBL wishes to warn sponsors of EMBL conferences and courses of fraudulent schemes purporting to offer sponsorship opportunities on behalf of EMBL or affiliated with EMBL officials. One current scam campaign of which we are aware is conducted using the name ‘Judy Eastman’ (judy@gopcontact.a2hosted.com) and entails approaches to sponsors offering sponsorship opportunities on EMBL’s behalf. Please be kindly advised that all relevant communication regarding sponsorship of EMBL conferences, symposia and courses is handled by EMBL directly and is sent from an official EMBL account. EMBL does not work with any external providers on sponsorship acquisition.

Please also note that:

  • EMBL never provides attendee lists for purchase. Any offers of such are fraudulent.
  • EMBL will never call or email you to ask for your credit card details or to request a payment.
  • All payments are on invoice.

Suspicious communications purportedly from, for or on behalf of EMBL should be reported to EMBL at the following email address sponsorship@embl.de.

Media kit

Want to let others know you’re attending this event? Take a look at our shareable media and feel free to use them in your social media channels or presentations.

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Date: 22 - 27 Oct 2023

Location: EMBL Heidelberg and Virtual

Venue: EMBL Advanced Training Centre


Deadline(s):

Application: Closed

Application: Closed


Organisers:

  • Monica Ballarino
    University of Rome, La Sapienza, Italy

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