
Monica Ballarino
University of Rome, La Sapienza
Italy
EMBO Practical Course
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Over the past decades it became evident that a RNA transcripts specific amount and localisation are important in order to understand the biological function of its (non-) coding gene. Whereby state-of-the-art in situ hybridisation (ISH) technologies are key to visualise these RNAs in a given tissue or cell.
This course aims to highlight three powerful RNA visualisation techniques in histological tissue samples. During this course, the participants will learn to validate the cellular localisation of candidate genes from transcriptome studies through conventional ISH and, the more recent cutting-edge, smFISH (Single Molecule Fluorescent ISH) and HCR-ISH (Hybridization Chain Reaction), based RNA ISH hybridisation methods. Additionally, the course will also cover essential tissue embedding protocols that are adapted for use in visually interrogating RNA transcripts in mammalian tissues.
This course is directed to early stage scientists (PhD and Postdocs) from the fields of RNA and molecular biology as well as various fields with an interest in these methodologies.
University of Rome, La Sapienza
Italy
University of Trento
Italy
University of Nice, Institut de Biologie Valrose (iBV)
France
EMBL Heidelberg
Germany
University of Rome, La Sapienza
Italy
Friedrich Miescher Institute for Biomedical Research
Switzerland
Max Delbrück Center for Molecular Medicine
Germany
Center for Life Nano- & Neuro-Science of Istituto Italiano di Tecnologia (IIT)
Italy
Münster University Hospital
Germany
Institute of Microbiology Heinrich Heine University
Germany
Turbine.ai
Hungary
Princeton University
USA
EMBL Heidelberg
Germany
Max Planck Institute of Biochemistry
Germany
EMBL Rome
Italy
University of Rome, La Sapienza
Italy
Turbine.ai
Hungary
EMBL Rome
Italy
Course and Conference Officer
EMBL Heidelberg
Germany
Training Lab Technician
EMBL Heidelberg
Germany
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Date: 22 - 27 Oct 2023
Location: EMBL Heidelberg
Venue: EMBL Advanced Training Centre
Organisers:
Contact: Charlotte Courtney
EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme