Edit
Extracellular vesicles: from biology to biomedical applications – Course and Conference Office

EMBO Practical Course

Extracellular vesicles: from biology to biomedical applications

Overview

Please see EMBL’s COVID-19 safety policy for testing and other requirements to attend this on-site course.

Course overview

A rapidly increasing number of researchers aim to purify and identify extracellular vesicles and many methodologies to isolate and analyse these vesicles are available and have been implemented. This course will cover the basics, possibilities, and limitations of different purification and characterisation techniques and will address strategies to understand their role in biomedical applications.

Audience

This course is intended for PhD students and Postdoctoral researchers who are interested in learning how to work with extracellular vesicles and know more about their biomedical applications.

Modules/resources

  • Extracellular vesicles (continuous and discontinuous density gradient ultracentrifugation, ultrafiltration techniques, bead-capture and precipitation techniques, size-exclusion chromatography (SEC))
  • Extracellular vesicles characterisation (Immunoblot and electron microscopy, fluorescent labeling, quantitative and qualitative analysis by flow cytometry, nanoparticle tracking analysis, in vivo imaging, proteomic and transcriptomic analysis of EV)
  • Extracellular vesicles composition (lipidomics, proteomics, transcriptomics)
  • Extracellular vesicles and function (molecular players for extracellular vesicle biogenesis, extracellular vesicle targeting to cells, extracellular vesicles as diagnostics and/or therapeutics in pathophysiology)

Learning outcomes

In this course we intend to make PhD students and post-doctoral researchers familiar with good-practice-guidelines and methods for purification and identification of extracellular vesicles with practical sessions included.

What past participants say about the course

“This course was very informative and covers all the major aspects of EV research. It addresses questions of what level of quality is required for good publications but also offers relevant approaches towards analysing data and critical thinking in the EV field. Whether you are taking your first steps in this field or more experienced, this course applies to you.” – Dr. Berglind Eva Benediktsdóttir, University of Iceland, Iceland

“My research is focused on understanding the role of exosomes in the progression of glioblastoma. While working on exosome-mediated regulation of angiogenesis in glioblastoma, I found significant difficulties in getting a pure population of exosomes as it is often contaminated with other biomolecules. As a result, we were in delusion whether the data which we were getting was the effect of exosomes or other biomolecules. Fortunately, I found the EMBO Practical Course on extracellular vesicles, focusing on the isolation and purification of extracellular vesicles, which was extremely important for my research work. We were trained to use cutting-edge research tools for extracellular vesicles and learned bioinformatic analysis of biomolecules that are present in exosomes. I also got an opportunity to interact with world-leading researchers and discuss my research. ” – Abir Mondal, National Centre for Cell Science, India

Speakers

Speakers, trainers, and staff

Scientific organisers

Course organisers

Raili Pall

Course and Conference Officer

EMBL Heidelberg

Germany

Preliminary programme

Got something to say? Tweet it! #EMBOEVs 

  • Some pre-course work (such as watching pre-recordings, protocol reading and computational exercises) with estimated time 0-10 hours will be required and will be shared in advance of the course.
  • Some speakers may need to join virtually to give their lecture or Q&A, and the programme is subject to change.
Day 1 – Sunday 12 March 2023
Time (Europe/Berlin)SpeakerLocation
16:00 – 16:30Registration ISG Hotel
16:30 – 17:00Arrival & Welcome ISG Hotel
17:00 – 19:00Ice BreakerISG Hotel
19:00Buffer dinner & NetworkingISG Hotel
Day 2 – Monday 13 March 2023
Time (Europe/Berlin)SpeakerLocation
08:40Bus from ISG hotel to EMBLISG hotel
bus stop
09:00 – 09:15 Outline of the day
An Hendrix – Ghent University, Belgium
Courtyard
Room A & B
09:15 – 10:00Lecture: Heterogeneity in EV methods
An Hendrix – Ghent University, Belgium
Courtyard
Room A & B
10:00 – 10:30Coffee breakFlex Lab
A & B
10:30 – 11:15Lecture: Heterogeneity in EV subtypes
Edit Buzas – Semmelweis University, Hungary
Courtyard
Room A & B
11:15 – 11:45Introduction to practicals and
separation methods

An Hendrix – Ghent University, Belgium
Courtyard
Room
A & B
11:45 – 12:45Lunch break EMBL Canteen
12:45 – 13:45Practical 1: Preparation of EVs by
differential ultracentrifugation
Training
Lab A & B
13:45 – 14:30Lecture: Fluorescent labeling of EVs
Esther Nolte-’t Hoen – Utrecht University,
The Netherlands
Courtyard
Room A & B
14:30 – 15:30Practical 1: Fluorescent labeling
Trainers: An Hendrix, Esther Nolte-’t
Hoen, Cecilia Lässer, Cláudio Pinheiro,
Estefania Lozano Andrés, Maribel Lara Corona
Training
Lab A & B
15:30 – 16:15Lecture: EV biogenesis
Guillaume Van Niel – Institute of Psychiatry
and Neuroscience of Paris, France
Courtyard
Room A & B
16:15 – 16:45Coffee breakFlex Lab A & B
16:45 – 17:30Lecture: RNA sorting in EVs
Esther Nolte-’t Hoen – Utrecht University,
The Netherlands
Courtyard Room
A & B
17:30 – 19:45Practical 2: EV separation using Optiprep
density gradient centrifugation
Trainers: An Hendrix, Esther Nolte-’t
Hoen, Cecilia Lässer, Cláudio Pinheiro,
Estefania Lozano Andrés, Maribel Lara Corona
Training
Lab A & B
19:45 – 20:45DinnerEMBL canteen
20:45Bus from EMBL to ISG hotelEMBL bus
stop
Day 3 – Tuesday 14 March 2023
Time (Europe/Berlin)SpeakerLocation
08:40Bus from ISG hotel to EMBLISG hotel
bus stop
09:00 – 09:15Outline of the day
Guillaume Van Niel – Institute of Psychiatry and Neuroscience of Paris, France
Courtyard Room
A & B
09:15 – 10:25 Practical 3: EV separation using
size exclusion chromatography
Trainers: An Hendrix, Esther Nolte-’t Hoen, Cecilia Lässer, Cláudio Pinheiro, Estefania Lozano Andrés,
Maribel Lara Corona
Training
Lab A & B
10:25 – 10:55 Coffee breakFlex Lab
A & B
10:55 – 12:00 Practical 2 (continuation): Collect and process fractions
from density gradients
Trainers: An Hendrix, Esther Nolte-’t Hoen,
Cecilia Lässer, Cláudio Pinheiro, Estefania Lozano Andrés,
Maribel Lara Corona
Training
Lab A & B
12:00 – 12:45Lecture: Quantitative and qualitative
EV analysis by flow cytometry
Marca Wauben – Utrecht University,
The Netherlands
Courtyard Room
A & B
12:45 – 13:45Lunch breakEMBL Canteen
13:45 – 14:30Lecture: Single EV analysis
André Görgens – Karolinska Institutet, Sweden
Courtyard Room
A & B
14:30 – 16:00 Practical 4: Particle analysis
Trainers: An Hendrix, Esther Nolte-’t Hoen, Cecilia Lässer,
Cláudio Pinheiro, Estefania Lozano Andrés,
Maribel Lara Corona
Training Lab
A & B
16:00 – 16:30 Coffee breakFlex Lab
A & B
16:30 – 18:45 Practical 4 (continuation): Particle analysis
Trainers: An Hendrix, Esther Nolte-’t Hoen,
Cecilia Lässer, Cláudio Pinheiro, Estefania Lozano Andrés,
Maribel Lara Corona
Training Lab
A & B
18:45 – 19:30 Dinner ATC Foyer
19:30 – 21:30Poster session I (ODD numbers) with drinksATC Foyer
21:30Bus from EMBL to ISG hotelEMBL bus stop
Day 4 – Wednesday 15 March 2023
Time (Europe/Berlin)SpeakerLocation
08:40Bus from ISG hotel to EMBLISG hotel bus stop
09:00 – 09:15Outline of the day
Esther Nolte-’t Hoen – Utrecht University,
The Netherlands
Courtyard Room
A & B
09:15 – 10:15Exercise 1: All Data AnalysisCourtyard Room
A & B
10:15 – 10:45Coffee break Flex Lab
A & B
10:45 – 11:45Lecture: EV characterization by transmission electron microscopy and western blot analysis
Guillaume Van Niel – Institute of Psychiatry
and Neuroscience of Paris, France
Courtyard Room
A & B
11:45 – 12:00Intro to practical session:
Guillaume Van Niel – Institute of Psychiatry
and Neuroscience of Paris, France
Courtyard Room
A & B
12:00 – 12:45Group activity: Tour of electron microscopy
core facility with Q&A session
12:45 – 13:45Lunch breakEMBL Canteen
13:45 – 17:15Practical sessions 5,6,7 in parallel (with flexible coffee break)
Group 1: EM Prep – Maribel Lara Corona
Group 2: EM remote – Guillaume Van Niel
Group 3: EV Track – An Hendrix, Cláudio Pinheiro
Training Lab A & B
Group 1: EM Prep – Maribel Lara Corona
Group 2: EM remote – Guillaume Van Niel
Group 3: EV Track – An Hendrix, Cláudio Pinheiro
Training Lab A & B
Group 1: EM Prep – Maribel Lara Corona
Group 2: EM remote – Guillaume Van Niel
Group 3: EV Track – An Hendrix, Cláudio Pinheiro
Training Lab A & B
17:15 – 18:15Q&A SessionCourtyard Room
A & B
18:15 – 19:00Pizza DinnerATC Foyer
19:00 – 21:00Poster session II (EVEN numbers) with drinksATC Foyer
21:00Bus from EMBL to ISG hotelEMBL bus stop
Day 5 – Thursday 16 March 2023
Time (Europe/Berlin)SpeakerLocation
08:40Bus from ISG hotel to EMBLISG hotel bus stop
09:00 – 09:15Outline of the day
Cecilia Lässer – University of Gothenburg, Sweden
Courtyard Room
A & B
09:15 – 10:00Lecture: Impact of pre-analytical factors on EV separation
Cecilia Lässer – University of Gothenburg, Sweden
Courtyard Room
A & B
10:00 – 11:15Practical 8: Data Analysis
Trainers: An Hendrix, Esther Nolte-’t Hoen, Cecilia Lässer, Cláudio Pinheiro, Estefania Lozano Andrés, Maribel Lara Corona, Guillaume Van Niel
Training
Lab A & B
11:15 – 11:45Coffee breakFlex Lab
A & B
11:45 – 12:45Practical 8 (continuation): Data Analysis
Trainers: An Hendrix, Esther Nolte-’t Hoen, Cecilia Lässer, Cláudio Pinheiro, Estefania Lozano Andrés, Maribel Lara Corona, Guillaume Van Niel
Training
Lab A & B
12:45 – 13:45Lunch break EMBL Canteen
13:45 – 15:45Practical 8 (continuation): Presentation data analysis and discussionCourtyard Room
A & B
15:45 – 16:15Coffee breakFlex Lab
A & B
16:15 – 17:00Lecture: Functional EV analysis in vivo
Michiel Pegtel – Amsterdam UMC, The Netherlands
Courtyard Room
A & B
17:00Bus from EMBL to ISG HotelEMBL bus stop
Free evening
Day 6 – Friday 17 March 2023
Time (Europe/Berlin)SpeakerLocation
08:40Bus from ISG hotel to EMBLISG hotel
bus stop
09:00 – 09:15 Outline of the day
An Hendrix – Ghent University, Belgium
Courtyard
Room
A & B
09:15 – 10:00Lecture: Transcriptomics
Jo Vandesompele – Ghent University, Belgium
Courtyard
Room
A & B
10:00 – 10:45Lecture: Separation of EVs from different biofluids
Olivier De Wever – Ghent University, Belgium
Courtyard
Room
A & B
10:45 – 11:15Coffee breakFlex Lab
A & B
11:15 – 12:00Lecture: Proteomics
Suresh Mathivanan – La Trobe University,
Australia
Courtyard
Room
A & B
12:00 – 13:00Lunch breakEMBL Canteen
13:00 – 15:30Practical 9: Practical/Bioinformatics
Suresh Mathivanan – La Trobe University,
Australia
Computer Training
Lab
15:30 – 16:00Coffee breakFlex Lab
A & B
16:00 – 16:45Lecture: Lipidomics
Johan Swinnen – KU Leuven, Belgium
Courtyard
Room
A & B
16:45 – 17:30Lecture: Clinical application
Mario Gimona – Paracelsus Medical University, Austria
Courtyard
Room A & B
17:30 – 18:30‘Meet-the-speakers’ sessionCourtyard Room
A & B
18:30 – 19:00Wrap up sessionCourtyard Room
A & B
19:00Bus from EMBL to NeckarmünzplatzEMBL bus stop
19:45Course dinner downtown

Practical information

Registration fees and motivation letter

The course is limited to 24 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.

Registration

Registration fees include admission, course materials, COVID-19 safety measures, meals and coffee breaks.
This EMBO course includes accommodation and transportation to and from the ISG Hotel to the venue.

Academia€500
PhD Student€500
Industry€1000

NO visa support letters will be issued until payment of the registration fee is confirmed.

Confirmation and payment

The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.

Motivation letter submission

After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.

Instructions

  1. Include your relevant skills, experience and qualifications showing why you would be suitable for the course.
  2. Explain why you would like to attend, including what you can contribute and how you think you will benefit from the course.

Please note:

  • The limit of 2000 characters refers to manually typed text and excludes spaces. If an error occurs try using a different web browser (preferably Google Chrome or Mozilla Firefox).
  • If you copy-paste the text into the form, hidden formatting might still be included which may cause the text to exceed the 2,000 character limit resulting in an error message. We recommend you clear all formatting before pasting in the text.
  • If you have special symbols in your text, make sure you are using Unicode characters, otherwise these will not be recognised.

For detailed instructions, please watch our video on how to submit a course motivation letter.

Further details

For further information about registration and motivation letter submission please refer to the FAQ page.

Financial assistance

Financial assistance

Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of both registration fee waivers and travel grants. Availability is limited to participants attending on-site EMBL Courses and virtual or on-site EMBO Practical Courses in Heidelberg, and will be indicated during the motivation letter submission process.

Your place in the course is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.

Registration fee waiver

The fee waiver will cover the registration sum that you have paid to attend the course.

Travel grant

The travel grant will cover the cost of travel (airfare, train, bus, taxi, accommodation, visa, and/or registration fees*) and is provided up to specified caps which are normally as follows:

– up to €400 for participants travelling to an EMBL Course within Europe.

– up to €1000 for participants travelling to an EMBL Course from outside Europe.

– up to €500 for any participant travelling to an EMBO Practical Course.

– up to €1000 for any participant working in Chile, India, Singapore or Taiwan travelling to an EMBO Practical Course.

– up to €700 for any participant working in Croatia, Czech Republic, Estonia, Greece, Hungary, Italy, Lithuania, Luxembourg, Poland, Slovenia, and Turkey travelling to an EMBO Practical Course.

*Registration fees are only covered for EMBO Practical Courses

The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.

Childcare grant (EMBO Practical Courses only)

For EMBO Practical Course participants with children, there is the possibility to apply for a childcare grant to offset child care costs incurred by participants or speakers when participating at a course. Eligible costs include fees for a babysitter or child-care facility, travel costs for a caregiver, or travel costs for taking the child to the meeting etc. Please note that priority will be given to early-stage researchers. A maximum amount of €500 can be awarded per participant applying for an EMBO childcare grant. 

Boehringer Ingelheim Fonds travel grant

Applies to selected courses only. Availability will be indicated during the motivation letter submission process.

This grant covers costs related to your attendance at the course (registration, travel and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.

Whether you are eligible to apply for a travel grant, depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):

– for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course

– for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course

Application

You may apply for financial assistance when submitting your motivation letter for courses. In your application, you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.

For the Boehringer Ingelheim Fonds Travel Grant, there is a pre-application question during the motivation letter submission process, and if selected you will be requested to complete a standard form and documentation consisting of your travel expense estimation.

Selection

The scientific organisers will select the recipients of all financial assistance during the motivation letter selection process. Results will be announced approximately 6-8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the course. Selection is based on scientific merit, your current work or study location, the reasons for needing financial support, and the impact this event will have on your career.

Reimbursement

Costs will be reimbursed after the meeting only once a reimbursement form and original receipts (from travel costs) have been received.

Further details

See our list of external funding opportunities and for further information about financial assistance please refer to the FAQ page.

Accommodation and shuttles

Hotel

Accommodation has been pre-booked for you at the ISG Hotel for the default dates of Sunday 12 March to Saturday 18 March (6 nights). Please note that the course registration fee includes coverage of accommodation costs in twin rooms, including breakfasts, for your stay at the ISG. In case you would like to have a single room, please let us know and please note that you will be required to pay an additional cost of 21€ per night. This should be paid by you directly to the ISG during your stay. Any additional nights need to be booked and paid for by you. We recommend that all course participants stay at the ISG Hotel which is the dedicated hotel for the course.

Local transport

Shuttle buses will go from the ISG Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.

Further details

Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

For further information on getting to EMBL Heidelberg visit our Travel Information page.

For enquiries about accommodation and local transportation please refer to the FAQ page.

Technical requirements and event software

Technical requirements

Below you will find the software and technical requirements for this course.

  • Participants need to bring their own laptops, which will be used to remotely access EMBL virtual machines with pre-installed software.
  • You will need admin rights to install the software required to access the virtual machines. If you do not have admin rights, please contact your technical support team in advance to make sure the software can be installed.
  • If you have a non-standard HDMI port (other than type A), you need to bring an adaptor.
  • Keyboard and PC mouse are available for use with a standard USB port. If you have a different port type, such as USB-C, you will need to bring your own.

Event software

The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus. 

Additional information

Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.

COVID-19 related questions

COVID-19 information for on-site events at EMBL Heidelberg can be found in our COVID-19 FAQs.

Sponsors

Event supporter:

Sponsorship opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other conferences, or contact sponsorship@embl.de for further information on sponsoring possibilities.

If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.

Warning

EMBL wishes to warn sponsors of EMBL conferences and courses of fraudulent schemes purporting to offer sponsorship opportunities on behalf of EMBL or affiliated with EMBL officials. One current scam campaign of which we are aware is conducted using the name ‘Judy Eastman’ (judy@gopcontact.a2hosted.com) and entails approaches to sponsors offering sponsorship opportunities on EMBL’s behalf. Please be kindly advised that all relevant communication regarding sponsorship of EMBL conferences, symposia and courses is handled by EMBL directly and is sent from an official EMBL account. EMBL does not work with any external providers on sponsorship acquisition.

Please also note that:

  • EMBL never provides attendee lists for purchase. Any offers of such are fraudulent.
  • EMBL will never call or email you to ask for your credit card details or to request a payment.
  • All payments are on invoice.

Suspicious communications purportedly from, for or on behalf of EMBL should be reported to EMBL at the following email address sponsorship@embl.de.

Media kit

Want to let others know you’re attending this event? Take a look at our shareable media and feel free to use them in your social media channels or presentations.

Social 1080×1920
Social 1080×1080
Social 1600×900
PPT Slide 16×9

Date: 12 - 17 Mar 2023

Location: EMBL Heidelberg

Venue: EMBL Advanced Training Centre


Deadline(s):

Application: 8 Jan 2023


Organisers:

  • An Hendrix
    Ghent University, Belgium
    • Esther Noltet Hoen
      Utrecht University, Netherlands
      • Guillaume Van Niel
        INSTITUTE OF PSYCHIATRY AND NEUROSCIENCE OF PARIS, France

      Contact: Raili Pall

      Download event poster


EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme

Founder partners

Corporate partners

Associate partners

Edit