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Hands-on flow cytometry: learning by doing! – Course and Conference Office

EMBL Course

Hands-on flow cytometry: learning by doing!

Overview

Course overview

Flow cytometry is a highly effective method for analysing individual cells that has become widely used in the fields of life sciences and medicine. By utilising flow cytometry, researchers can accurately identify and measure specific cell populations within complex mixtures, as well as assess cell cycle progression, proliferation status, and expression of fluorescent markers.

Our course aims to provide a comprehensive understanding of the underlying principles of flow cytometry, while also emphasising practical considerations related to the most frequently used assays. Through a combination of theoretical instruction and hands-on training, students will gain the knowledge and skills necessary to effectively design, execute, and interpret flow cytometry experiments.

Audience

The course is designed for researchers at any step of their career and will cover the subject from basic principles to complex assays. Prior basic working knowledge of flow cytometry, data acquisition or analysis software (DIVA + FlowJo)  is of advantage, but not strictly a must.

Modules/resources

  • In-depth principles of Flow Cytometry
  • Quality assessment and instrument validation
  • Analysis of cell cycle profiles in fixed cells
  • Particles counting
  • Apoptosis assays
  • Tissue staining of cell surface and intracellular antigens
  • Principles and guidelines for experimental design, especially for multi-colour Flow Cytometry
  • Essential of gating and gating controls
  • Detailed multi-sessions on analysing data from Flow Cytometers in cooperation with FlowJo
  • Guidelines to prepare flow cytometry data for publication 

Learning outcomes

Our goal for this course is to teach the basic of this technology with a very big emphasis on practical work at the instruments. Nothing is better than learning by doing! We have a great set of experienced trainers in this technology delivering lectures and accompanying you into the lab doing flow on your own. We will have support from FlowJo delivering the basics and some advanced features in multiple sessions. This in-depth five-day course will push your knowledge and skills preparing you for any flow projects ahead on your research path.

What past participants say about the course

My experience at EMBL was lovely and stimulating. The knowledge I acquired through this short time has improved my learning and practical skills. EMBL is a great place for scientific resources; a friendly environment both socially and professionally.” – Ibrahim Malami, Usmanu Danfodiyo University Sokoto

The course was excellently organized, with high quality theoretical and practical sessions! It is a must for everyone interested in basic and more specific aspects of flow cytometry techniques.” – Filipe Olim, University of Madeira

“I enjoyed the course a lot and learned what I was looking for: an opportunity to learn the principles of flow cytometry that are important for planning and performing experiments. Besides the theoretical part, I was able to run samples at the instruments by myself which was a great starting point to perform experiments by myself in the future.” – Maya Funk, German Cancer Research Center

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Please see EMBL’s COVID-19 safety policy for testing and other requirements to attend this on-site course.

Date: 13 - 17 Nov 2023

Location: EMBL Heidelberg

Venue: EMBL Advanced Training Centre


Deadline(s):

Closed


Organisers:


EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme

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