Advances in cryo-electron microscopy and 3D image processing

Overview

Course overview

Over the past decade, cryo-electron microscopy has transformed our understanding of biomolecular structures across multiple spatial scales from atoms to cells. Technological advances have provided unprecedented insights, from atomic-resolution structures of biologically heterogeneous states to the first glimpses into the molecular sociology of a cell. Yet, emerging breakthroughs, such as time-resolved approaches, novel (correlated) imaging technologies, and AI-driven image processing, highlight the potential of cryo-EM for another leap forward. To realise this potential, in-depth advanced training is essential for driving innovation and developing new technologies to tackle emerging biological challenges.

This course offers a comprehensive foundation in advanced single-particle and cellular tomography techniques, covering the entire workflow from sample preparation to data collection and image processing. Participants will have the chance to meet the leaders in the field, with unique 1:1 meetings and informal social programme.

Audience

Designed for PhD students and postdoctoral researchers with basic experience in cryo-EM and 3D image processing, the course will equip participants with the expertise to integrate state-of-the-art single-particle analysis and tomography into their research. It will also prepare them to critically discuss their knowledge with peers and ultimately contribute to the development of novel cryo-EM methodologies beyond the current state of the art.

Modules/resources

• Single particle reconstruction (cisTEM, Relion, cryoSPARC)
• Subtomogram averaging (RELION-5, Warp)
• Atomic models (model building and integrative modeling)
• Session at the microscopes (cryo-EM/ET and cryo-FIB)
• Latest developments in instrumentations
• Tomogram reconstruction, template matching

Learning outcomes

The course will enable participants to seamlessly integrate single particle analysis and tomography approaches to their cutting-edge research, disseminate their gained expertise to colleagues, and to innovate cryo-EM methods beyond the state-of-the-art.

What past participants say about the course

“The course was among the best I’ve attended on this topic. It was an exceptional opportunity for both learning and networking.” – Fernanda Sala, UMG and MPI, Germany

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Sponsors

Event sponsors

Sponsorship opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other events, or contact sponsorship@embl.org for further information.