Advances in cryo-electron microscopy and 3D image processing

Time (Europe/Berlin)	Session	Location
13:00 – 13:15	Bus from hotel to EMBL ATC Entrance	ISG Hotel
13:15 – 13:55	Registration and refreshments	Courtyard Room A & B
13:55 – 14:05	Introduction and housenote slides Sebastian Eustermann – EMBL Heidelberg, Germany	Courtyard Room A & B
14:05 – 14:15	Course overview	Courtyard Room A & B
14:15 – 15:15	Flash talks	Courtyard Room A & B
15:15 – 15:45	Coffee break	Courtyard Room A & B
15:45 – 16:45	Flash talks (continuation)	Courtyard Room A & B
16:45 – 18:00	Lecture: Future of cell structural biology Bridget Carragher – Chan Zuckerberg Institute for Advanced Biological Imaging, USA (Virtual speaker)	Courtyard Room A & B
18:00 – 19:00	Dinner	EMBL Canteen

08:20 – 08:30	Bus from hotel to EMBL ATC Entrance
08:30 – 08:35	Overview of the day	Courtyard Room A & B
08:35 – 09:00	Emerging technologies from leading R&D industry experts	Courtyard Room A & B
09:00 – 10:15	Lecture: Cryo-EM image formation and optics To be confirmed	Courtyard Room A & B
10:15 – 10:45	Coffee break sponsored by company	Courtyard Room A & B
10:45 – 12:00	Lecture: Next generation: microscope hardware Jürgen Plitzko – Max-PLanck Institute of Biochemistry, Germany	Courtyard Room A & B
12:00 – 13:00	Lunch	EMBL Canteen
13:00 – 14:15	Lecture: Perspectives on cryoEM single particle sample preparation & acquisition Kelly Nguyen – MRC Laboratory of Molecular Biology, United Kingdom	Courtyard Room A & B
14:15 – 15:30	Lecture: Perspectives on cryoET sample preparation & acquisition Julia Mahamid – EMBL Heidelberg, Germany	Courtyard Room A & B
15:30 – 16:00	Coffee break sponsored by company	Courtyard Room A & B
16:00 – 17:15	Lecture: Fundamentals in cryoEM image processing Sjors Scheres – MRC Laboratory of Molecular Biology, United Kingdom	Courtyard Room A & B
17:15 – 18:30	Lecture: Fundamentals in AI-approaches and cryoEM/ET data regularization Sjors Scheres – MRC Laboratory of Molecular Biology, United Kingdom	Courtyard Room A & B
18:30 – 19:30	Dinner	EMBL Canteen
19:30 – 20:30	Lecture: Time resolved cryoEM and novel avenues for cryoET Bonnie Murphy – Max-PLanck Institute of Biochemistry, Germany	Courtyard Room A & B

Time (Europe/Berlin)	Session	Location
08:20 – 08:30	Bus from hotel to EMBL ATC Entrance
08:30 – 08:35	Overview of the day	Courtyard Room A & B
08:35 – 08:55	Emerging technologies from leading R&D industry experts	Courtyard Room A & B
08:55 – 10:10	Lecture: Fundamentals in cryoET image processing Tanmay Bharat – MRC Laboratory of Molecular Biology, United Kingdom	Courtyard Room A & B
10:10 – 10:30	Coffee break sponsored by company	Courtyard Room A & B
10:30 – 11:45	Lecture: High resolution by cryo ET subtomogram averaging Dimitry Tegunov – Genentech, USA	Courtyard Room A & B
11:45 – 12:15	Lecture: Next generation: 2D template matching and beyond Nikolaus Grigorieff – University of Massachusetts Medical School, USA	Courtyard Room A & B
12:15 – 13:15	Lunch	EMBL Canteen
13:15 – 15:45	Practical session A Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic Dari Kimanius – Chan Zuckerberg Institute for Advanced Biological Imaging, USA Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic Industry trainer Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic Tanmay Bharat – MRC Laboratory of Molecular Biology, United Kingdom Bogdan Toader – MRC Laboratory of Molecular Biology, United Kingdom Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic Alister Burt – Genentech, USA Dimitry Tegunov – Genentech, USA Session 5 on Model building, refinement and validation Arjen Jakobi – Delft University of Technology, Netherlands	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix Seminar B Room
15:45 – 16:15	Coffee break sponsored by company	Courtyard Room A & B
16:15 – 18:45	Practical session B Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic Dari Kimanius – Chan Zuckerberg Institute for Advanced Biological Imaging, USA Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic Industry trainer Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic Tanmay Bharat – MRC Laboratory of Molecular Biology, United Kingdom Bogdan Toader – MRC Laboratory of Molecular Biology, United Kingdom Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic Alister Burt – Genentech, USA Dimitry Tegunov – Genentech, USA Session 5 on Model building, refinement and validation Arjen Jakobi – Delft University of Technology, Netherlands	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix Seminar Room B
18:45 – 19:45	Dinner	EMBL Canteen
19:45 – 20:45	Lecture: Hot topics: recent breakthroughs and emerging technologies Lori Passmore – MRC Laboratory of Molecular Biology, United Kingdom	Courtyard Room A & B
20:45 – 21:15	Discussion round – Hot topics: recent breakthroughs and emerging technologies	Courtyard Room A & B

Time (Europe/Berlin)	Session	Location
08:20 – 08:30	Bus from hotel to EMBL ATC Entrance
08:30 – 08:35	Overview of the day	Courtyard Room A & B
08:35 – 09:50	Lecture: Advances in cryoET particle identification Friedrich Förster – Utrecht University, Netherlands	Courtyard Room A & B
09:50 – 10:10	Coffee break sponsored by company	Courtyard Room A & B
10:10 – 11:25	Lecture: Molecular heterogeneity landscapes from cryoEM/ET Anna Kreshuk – EMBL Heidelberg, Germany	Courtyard Room A & B
11:25 – 12:25	Technology Lunch sponsored by company	Courtyard Room Foyer
12:25 – 12:55	Panel discussion: Software and image processing Panel leaders: Sebastian Eustermann – EMBL Heidelberg, Germany and Tanmay Bharat – MRC Laboratory of Molecular Biology, United Kingdom	Courtyard Room A & B
12:55 – 15:25	Practical session C Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic Dari Kimanius – Chan Zuckerberg Institute for Advanced Biological Imaging, USA Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic Industry trainer Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic Tanmay Bharat – MRC Laboratory of Molecular Biology, United Kingdom Bogdan Toader – MRC Laboratory of Molecular Biology, United Kingdom Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic Alister Burt – Genentech, USA Dimitry Tegunov – Genentech, USA Session 5 on Model building, refinement and validation Arjen Jakobi – Delft University of Technology, Netherlands	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix B Seminar Room
15:25 – 15:55	Coffee break sponsored by company	Courtyard Room A & B
15:55 – 18:25	Practical session D Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic Dari Kimanius – Chan Zuckerberg Institute for Advanced Biological Imaging, USA Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic Industry trainer Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic Tanmay Bharat – MRC Laboratory of Molecular Biology, United Kingdom Bogdan Toader – MRC Laboratory of Molecular Biology, United Kingdom Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic Alister Burt – Genentech, USA Dimitry Tegunov – Genentech, USA Session 5 on Model building, refinement and validation Arjen Jakobi – Delft University of Technology, Netherlands	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix B Seminar Room
18:25 – 19:25	Pizza dinner	Helix B
19:25 – 20:25	Poster session 1	Helix B

08:20 – 08:30	Bus from hotel to EMBL ATC Entrance
08:30 – 08:35	Overview of the day	Courtyard Room A & B
08:35 – 09:35	Lecture: Next generation: Atomic model building, refinement and validation Arjen Jakobi -Delft University of Technology, The Netherlands	Courtyard Room A & B
09:35 – 10:35	Lecture: Next generation: integrative model building Jan Kosinski – EMBL Hamburg, Germany	Courtyard Room A & B
10:35 – 11:05	Coffee break kindly sponsored by company	Courtyard Room A & B
11:05 – 12:05	EMDB and EMPIAR Data repositories Kyle Morris – EMBL-EBI, United Kingdom	Courtyard Room A & B
12:05 – 12:35	Panel discussion: Validation and FAIR data Panel leader: Kelly Nguyen – MRC Laboratory of Molecular Biology, United Kingdom and Sebastian Eustermann – EMBL Heidelberg, Germany	Courtyard Room A & B
12:35 – 13:35	Technology Lunch sponsored by company	Courtyard Room A & B
13:35 – 16:05	Practical session E Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic Dari Kimanius – Chan Zuckerberg Institute for Advanced Biological Imaging, USA Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic Industry trainer Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic Tanmay Bharat – MRC Laboratory of Molecular Biology, United Kingdom Bogdan Toader – MRC Laboratory of Molecular Biology, United Kingdom Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic Alister Burt – Genentech, USA Dimitry Tegunov – Genentech, USA Session 5 on Model building, refinement and validation Arjen Jakobi – Delft University of Technology, Netherlands Session 6 on Macromolecular Heterogeneity Analysis Sebastian Eustermann – EMBL Heidelberg, Germany Session 7 on 2D template matching Johannes Elferich – University of Massachusetts Medical School, USA Nikolaus Grigorieff – University of Massachusetts Medical School, USA	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix B Seminar Room Session 6: B11 Session 7: B28
16:05 – 16:35	Coffee break sponsored by company	Courtyard Room A & B
16:35 – 18:05	Practical session F Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic Dari Kimanius – Chan Zuckerberg Institute for Advanced Biological Imaging, USA Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic Industry trainer Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic Tanmay Bharat – MRC Laboratory of Molecular Biology, United Kingdom Bogdan Toader – MRC Laboratory of Molecular Biology, United Kingdom Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic Alister Burt – Genentech, USA Dimitry Tegunov – Genentech, USA Session 5 on Model building, refinement and validation Arjen Jakobi – Delft University of Technology, Netherlands Session 6 on Macromolecular Heterogeneity Analysis Sebastian Eustermann – EMBL Heidelberg, Germany Session 7 on 2D template matching Johannes Elferich – University of Massachusetts Medical School, USA Nikolaus Grigorieff – University of Massachusetts Medical School, USA	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix Seminar Room B Session 6: B11 Session 7: B28
18:05 – 19:05	Lecture: Advances in AI-based image processing and modelling of supra-molecular assemblies Martin Beck – Max Planck Institute of Biophysics, Germany	Courtyard Room A & B
19:05 – 20:05	Dinner + Poster session 2	Helix B

Time (Europe/Berlin)	Session	Location
08:55 – 09:05	Bus from hotel to EMBL ATC Entrance
09:05 – 09:10	Overview of the day	Courtyard Room A & B
09:10 – 10:25	Lecture: High Throughput and Resolution Data collection Sonja Welsch– EMBL Heidelberg, Germany	Courtyard Room A & B
10:25 – 11:40	Lecture: Correlative light and electron microscopy for Structural Cell Biology Wanda Kukulski – University of Bern, Switzerland	Courtyard Room A & B
11:40 – 12:00	Coffee break sponsored by company	Courtyard Room A & B
12:00 – 12:30	Panel discussion: Data acquisition Panel leader: Simone Mattei – EMBL Heidelberg, Germany	Courtyard Room A & B
12:30 – 13:30	Lunch	EMBL Canteen
13:30 – 16:00	Practical session G Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic Dari Kimanius – Chan Zuckerberg Institute for Advanced Biological Imaging, USA Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic Industry trainer Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic Tanmay Bharat – MRC Laboratory of Molecular Biology, United Kingdom Bogdan Toader – MRC Laboratory of Molecular Biology, United Kingdom Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic Alister Burt – Genentech, USA Dimitry Tegunov – Genentech, USA Session 5 on Model building, refinement and validation Arjen Jakobi – Delft University of Technology, Netherlands Session 6 on Macromolecular Heterogeneity Analysis Sebastian Eustermann – EMBL Heidelberg, Germany Session 7 on 2D template matching Johannes Elferich – University of Massachusetts Medical School, USA Nikolaus Grigorieff – University of Massachusetts Medical School, USA	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix Seminar Room B Session 6: B11 Session 7: B28
16:00 – 16:30	Coffee break sponsored by company	Courtyard Room A & B
16:30 – 19:00	Practical session H Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic Dari Kimanius – Chan Zuckerberg Institute for Advanced Biological Imaging, USA Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic Industry trainer Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic Tanmay Bharat – MRC Laboratory of Molecular Biology, United Kingdom Bogdan Toader – MRC Laboratory of Molecular Biology, United Kingdom Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic Alister Burt – Genentech, USA Dimitry Tegunov – Genentech, USA Session 5 on Model building, refinement and validation Arjen Jakobi – Delft University of Technology, Netherlands Session 6 on Macromolecular Heterogeneity Analysis Sebastian Eustermann – EMBL Heidelberg, Germany Session 7 on 2D template matching Johannes Elferich – University of Massachusetts Medical School, USA Nikolaus Grigorieff – University of Massachusetts Medical School, USA	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix Seminar Room B Session 6: B11 Session 7: B28
19:00 – 20:00	Dinner	EMBL Canteen
20:00 – 20:30	Lecture: cryoEM careers in academia and pharma Alexis Rohou – Genentech, USA	Courtyard Room A & B
20:30 – 21:30	Discussion round: Career paths	Courtyard Room A & B

Time (Europe/Berlin)	Session	Location
08:55 – 09:05	Bus from hotel to EMBL ATC Entrance
09:05 – 09:10	Overview of the day	Courtyard Room A & B
09:10 – 11:10	Practical Session I – Drop in session Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic Dari Kimanius – Chan Zuckerberg Institute for Advanced Biological Imaging, USA Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic Industry trainer Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic Tanmay Bharat – MRC Laboratory of Molecular Biology, United Kingdom Bogdan Toader – MRC Laboratory of Molecular Biology, United Kingdom Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic Alister Burt – Genentech, USA Dimitry Tegunov – Genentech, USA Session 5 on Model building, refinement and validation Arjen Jakobi – Delft University of Technology, Netherlands Session 6 on Macromolecular Heterogeneity Analysis Sebastian Eustermann – EMBL Heidelberg, Germany Session 7 on 2D template matching Johannes Elferich – University of Massachusetts Medical School, USA Nikolaus Grigorieff – University of Massachusetts Medical School, USA Session 8 on Cryo-TEM Simon Fromm – EMBL Heidelberg, Germany Session 9 on Cryo-LM Zhengyi Yang – EMBL Heidelberg, Germany Session 10 on HPF/Waffle prep Trainer – EMBL Heidelberg, Germany Session 11 on FIB-SEM Trainer – EMBL Heidelberg, Germany	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix B Seminar Room Session 6: B11 Session 7: B28 Session 8, 9, 10: Imaging Center
11:10 – 12:10	Lunch break	EMBL Canteen
12:10 – 14:10	Practical Session J – Drop in session Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic Dari Kimanius – Chan Zuckerberg Institute for Advanced Biological Imaging, USA Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic Industry trainer Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic Tanmay Bharat – MRC Laboratory of Molecular Biology, United Kingdom Bogdan Toader – MRC Laboratory of Molecular Biology, United Kingdom Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic Alister Burt – Genentech, USA Dimitry Tegunov – Genentech, USA Session 5 on Model building, refinement and validation Arjen Jakobi – Delft University of Technology, Netherlands Session 6 on Macromolecular Heterogeneity Analysis Sebastian Eustermann – EMBL Heidelberg, Germany Session 7 on 2D template matching Johannes Elferich – University of Massachusetts Medical School, USA Nikolaus Grigorieff – University of Massachusetts Medical School, USA Session 8 on Cryo-TEM Simon Fromm – EMBL Heidelberg, Germany Session 9 on Cryo-LM Zhengyi Yang – EMBL Heidelberg, Germany Session 10 on HPF/Waffle prep Trainer – EMBL Heidelberg, Germany Session 11 on FIB-SEM Trainer – EMBL Heidelberg, Germany	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix B Seminar Room Session 6: B11 Session 7: B28 Session 8, 9, 10: Imaging Center
14:10 – 14:30	Coffee break	Courtyard Room A & B
14:30 – 16:30	Practical Session K – Drop in session Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic Dari Kimanius – Chan Zuckerberg Institute for Advanced Biological Imaging, USA Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic Industry trainer Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic Tanmay Bharat – MRC Laboratory of Molecular Biology, United Kingdom Bogdan Toader – MRC Laboratory of Molecular Biology, United Kingdom Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic Alister Burt – Genentech, USA Dimitry Tegunov – Genentech, USA Session 5 on Model building, refinement and validation Arjen Jakobi – Delft University of Technology, Netherlands Session 6 on Macromolecular Heterogeneity Analysis Sebastian Eustermann – EMBL Heidelberg, Germany Session 7 on 2D template matching Johannes Elferich – University of Massachusetts Medical School, USA Nikolaus Grigorieff – University of Massachusetts Medical School, USA Session 8 on Cryo-TEM Simon Fromm – EMBL Heidelberg, Germany Session 9 on Cryo-LM Zhengyi Yang – EMBL Heidelberg, Germany Session 10 on HPF/Waffle prep Trainer – EMBL Heidelberg, Germany Session 11 on FIB-SEM Trainer – EMBL Heidelberg, Germany	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix B Seminar Room Session 6: B11 Session 7: B28 Session 8, 9, 10: Imaging Center
16:30 – 18:30	Free time downtown
18:30 – 20:30	Downtown dinner	To be confirmed

Time (Europe/Berlin)	Session	Location
08:50 – 09:00	Bus from hotel to EMBL ATC Entrance
09:00 – 09:05	Overview of the day	Courtyard Room A & B
09:05 – 11:05	Practical Session L – Drop in session Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic Dari Kimanius – Chan Zuckerberg Institute for Advanced Biological Imaging, USA Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic Industry trainer Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic Tanmay Bharat – MRC Laboratory of Molecular Biology, United Kingdom Bogdan Toader – MRC Laboratory of Molecular Biology, United Kingdom Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic Alister Burt – Genentech, USA Dimitry Tegunov – Genentech, USA Session 5 on Model building, refinement and validation Arjen Jakobi – Delft University of Technology, Netherlands Session 6 on Macromolecular Heterogeneity Analysis Sebastian Eustermann – EMBL Heidelberg, Germany Session 7 on 2D template matching Johannes Elferich – University of Massachusetts Medical School, USA Nikolaus Grigorieff – University of Massachusetts Medical School, USA Session 8 on Cryo-TEM Simon Fromm – EMBL Heidelberg, Germany Session 9 on Cryo-LM Zhengyi Yang – EMBL Heidelberg, Germany Session 10 on HPF/Waffle prep Trainer – EMBL Heidelberg, Germany Session 11 on FIB-SEM Trainer – EMBL Heidelberg, Germany	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix B Seminar Room Session 6: B11 Session 7: B28 Session 8, 9: Imaging Center
11:05 – 12:05	Lunch	EMBL Canteen
12:05 – 14:05	Practical Session M – Drop in session Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic Dari Kimanius – Chan Zuckerberg Institute for Advanced Biological Imaging, USA Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic Industry trainer Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic Tanmay Bharat – MRC Laboratory of Molecular Biology, United Kingdom Bogdan Toader – MRC Laboratory of Molecular Biology, United Kingdom Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic Alister Burt – Genentech, USA Dimitry Tegunov – Genentech, USA Session 5 on Model building, refinement and validation Arjen Jakobi – Delft University of Technology, Netherlands Session 6 on Macromolecular Heterogeneity Analysis Sebastian Eustermann – EMBL Heidelberg, Germany Session 7 on 2D template matching Johannes Elferich – University of Massachusetts Medical School, USA Nikolaus Grigorieff – University of Massachusetts Medical School, USA Session 8 on Cryo-TEM Simon Fromm – EMBL Heidelberg, Germany Session 9 on Cryo-LM Zhengyi Yang – EMBL Heidelberg, Germany Session 10 on HPF/Waffle prep Trainer – EMBL Heidelberg, Germany Session 11 on FIB-SEM Trainer – EMBL Heidelberg, Germany	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix B Seminar Room Session 6: B11 Session 7: B28 Session 8, 9: Imaging Center
14:05 – 14:35	Coffee break	Courtyard Room A & B
14:35 – 16:35	Practical Session N – Drop in session Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic Dari Kimanius – Chan Zuckerberg Institute for Advanced Biological Imaging, USA Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic Industry trainer Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic Tanmay Bharat – MRC Laboratory of Molecular Biology, United Kingdom Bogdan Toader – MRC Laboratory of Molecular Biology, United Kingdom Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic Alister Burt – Genentech, USA Dimitry Tegunov – Genentech, USA Session 5 on Model building, refinement and validation Arjen Jakobi – Delft University of Technology, Netherlands Session 6 on Macromolecular Heterogeneity Analysis Sebastian Eustermann – EMBL Heidelberg, Germany Session 7 on 2D template matching Johannes Elferich – University of Massachusetts Medical School, USA Nikolaus Grigorieff – University of Massachusetts Medical School, USA Session 8 on Cryo-TEM Simon Fromm – EMBL Heidelberg, Germany Session 9 on Cryo-LM Zhengyi Yang – EMBL Heidelberg, Germany Session 10 on HPF/Waffle prep Trainer – EMBL Heidelberg, Germany Session 11 on FIB-SEM Trainer – EMBL Heidelberg, Germany	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix B Seminar Room Session 6: B11 Session 7: B28 Session 8, 9: Imaging Center
16:35 – 17:50	Poster session (all participants)	Helix B
17:50 – 18:50	Dinner	EMBL Canteen

Time (Europe/Berlin)	Session	Location
08:30 – 08:40	Bus from hotel to EMBL ATC Entrance
08:40 – 08:45	Overview of the day	Courtyard Room A & B
08:45 – 09:45	Lecture: Emerging technologies in structural biology via cryoET Svetlana Dodonova – EMBL Heidelberg, Germany	Courtyard Room A & B
09:45 – 10:45	Lecture: Hot topics time resolved cryoEM /cryoEM Bonnie Murphy Max Planck Institute of Biophysics, Germany Cristina Paulino – Heidelberg University, Germany	Courtyard Room A & B
10:45 – 11:45	Coffee break	Courtyard Room A & B
11:45 – 12:15	Wrap up practicals: highlights presented by participants	Courtyard Room A & B
12:15 – 13:15	Final panel discussion: Wrap up with emphasis on the future of cryoEM, responsible practice, reporting and validation standards	Courtyard Room A & B
13:15 – 13:30	Packed lunch and departure	Courtyard Room A & B

The course is limited to 24 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.

Registration fees

Your registration for the event includes:

• Admission to the event
• Meals and coffee breaks
• Course materials
• Access to the e-learning platform
• A letter to support your visa application (for more information, check the ‘Travel and accommodation’ tab)

For this EMBO course, the fees include accommodation (twin room) and transportation to and from the ISG Hotel to the venue

Academia	€550
PhD Student	€550
Industry	€1100

Confirmation and payment

The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.

Cancellation policy

If you are no longer able to take part in the course, please inform your course contact responsible for this event. Your registration and the submitted motivation letter will be deleted.

Please also check our Terms and Conditions for the cancellation policy.

After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.

Instructions

1. Include your relevant skills, experience and qualifications showing why you would be suitable for the course.
2. Explain why you would like to attend, including what you can contribute and how you think you will benefit from the course.

Please note:

• The limit of 2,000 characters refers to manually typed text and excludes spaces. If an error occurs try using a different web browser (preferably Google Chrome or Mozilla Firefox).
• If you copy-paste the text into the form, hidden formatting might still be included which may cause the text to exceed the 2,000 character limit resulting in an error message. We recommend you clear all formatting before pasting in the text.
• If you have special symbols in your text, make sure you are using Unicode characters, otherwise these will not be recognised.

For detailed instructions, please watch our video on how to submit a course motivation letter.

Please note it is not possible to apply after the motivation letter deadline!

Further details

For further information about registration and motivation letter submission please refer to the FAQ page.

Financial assistance

Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of travel grants, childcare grants, and accessibility grants.

Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.

Travel grant

The travel grant will cover the cost of travel (airfare, train, bus, taxi, and visa) and/or registration fees and is provided up to specified caps which are normally as follows:

• up to €500 for any participant travelling to an EMBO Practical Course.

• up to €1000 for any participant working in Chile, India, Singapore, or Taiwan travelling to an EMBO Practical Course.

The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.

Childcare grants

There is the possibility to apply for a childcare grant to offset child care costs incurred by participants, speakers, trainers, and organisers when attending a course. Eligible costs include (but are not limited to) fees for a babysitter, child-care facility, or travel costs for a caregiver. Please note that priority will be given to early-stage researchers. There is a limited amount of funding available for the childcare grants and funds will be distributed amongst eligible applicants.

Boehringer Ingelheim Fonds travel grant

Applies to selected courses only. Availability will be indicated during the abstract or motivation letter submission process.

This grant covers costs related to your attendance to the course (registration, travel, and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.
Whether you are eligible to apply for a travel grant, depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):

• for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course
• for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course

EMBO accessibility grant

For EMBO Practical Course participants with accessibility needs, there is the possibility to apply for an accessibility grant to offset costs incurred by participants or speakers when participating at a course. Eligible costs include costs incurred for equipment or adaptations to the course environment, or travel costs for an event companion to assist the participant or speaker during the course where necessary. While we do our best to accommodate needs, please note that extensive changes to the course environment might not be possible. Priority will be given to early-stage researchers.

A maximum amount of €500 can be awarded per participant applying for an EMBO accessibility grant. In order to apply for this grant for EMBO Practical Courses, you must be registered by the application deadline. 

Application

Whether you are attending onsite or virtually, to apply for financial assistance, please follow the steps in the application system. During the process of submitting your motivation letter to the system, you will be asked which type of grant you want to apply for.

Applications for financial support will not affect the outcome of your selection results.

Information about the grant will be sent out shortly after the motivation letter deadline.

Results

Grant allocations are announced by email to all applicants after the selection results.

Allocation results do not impact your selection to the course. Selection for financial assistance is based on scientific merit, your current work or study location, the reasons for needing financial support, and the impact this event will have on your career.

Please note that for childcare grants, priority will be given to early-stage researchers.

Further details

Check out this list of external funding opportunities. 

For further information about financial assistance please refer to the FAQ page.

The selection results will be announced by email to all applicants. We aim to announce 3 weeks (21 days) after the motivation letter submission deadline but delays may occur.

Visa

We can send you a registration confirmation to support your visa procedure (not an official invitation letter). Please contact the Conference Officer responsible for your event. Read our FAQ for more information.

Travel

If you are travelling to Heidelberg via aeroplane, train, car, or long-distance bus, you can find useful information on our travel information page.

Accommodation

Accommodation in twin rooms, with breakfast included in the registration fee. Accommodation will be booked for the selected participants at ISG Hotel for the default dates of 16 to 25 August 2026 (8 nights).

In case you would like to have a single room, please let the hotel know and please note that you will be required to pay a surcharge per night (availability of single rooms may be limited). This should be paid by you directly to the Hotel during your stay.

We recommend that all course participants stay at the ISG Hotel for the course.

Local transportation

Course shuttle buses

Shuttle buses will go from the ISG Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.

Other local transportation

In addition to the shuttle busses, there is also the public bus 28 that serves the EMBL campus and taxis can be easily booked at any time. For more information, please refer to the local transportation (RNV) website.

You may also walk from the ISG Hotel to EMBL (takes up to 30 minutes)

Map to walk from ISG Hotel to EMBL

Further details

Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

For more information about accommodation and travel, please refer to the FAQ page.

Here you will find useful information for when you are onsite at the event.

Catering

All meals and coffee breaks are included in the registration fee. Our catering staff will prepare a variety of vegetarian meals, meat and fish dishes, soups, pasta, fresh fruit and vegetables, as well as a variety of desserts.

Please wear your badge at all times when serving yourself.

No food or drinks are allowed in the laboratories.

Internet

Wi-Fi is available on campus using the EMBL-Events network and the event specific password, which will be provided on site. The eduroam network (secure, worldwide roaming access service developed for the international research and education community) is also available.

Photography

During the course a member of the Marketing Team may be taking photos. If you would not like to appear in these, please inform the photographer or a member of the Course and Conference Office.

Charging stations

There are lockers available next to the stairs leading down into the Auditorium. You will find some of those equipped with sockets to charge your smartphone/tablet etc.

In most places the electricity is 220 volts AC (50 cycles). An adaptor and a plug that fits the German socket may be needed for your appliances/laptop (i.e. American, Japanese, etc.).

Printing

We can help to print your boarding pass/train ticket. Please send it to events@embl.de and collect your print-outs at the registration desk.

Luggage

There are lockers available on-site to store your luggage, which require a 2 EURO coin to operate. There is another luggage room on level E0, which is free to use but remains unlocked during the course.

Lost and found

‘’Lost and Found’’ items are kept at the main reception.

Nursing room

There is a nursing room available in the ATC Rooftop Lounge on level A29.

Room for prayers, meditation, and yoga

There is a room for prayer, yoga, and meditation, located on level E0 behind the Auditorium. Please be respectful of others using the room.

EMBL campus map

Health and safety notes

Please take a moment to read our health and safety policy.

• Do not smoke in any EMBL building.
• Eating and drinking is prohibited in the Auditorium and all laboratories.
• Do not enter any restricted areas or the laboratories unless instructed to do so.

If first aid is required

• The first aid room is located next to the ATC Registration Desk (room 10- 205).
• Dial the Emergency number 222 from any EMBL internal phone only.
• Be prepared to answer the following questions: Where is the accident? What happened? How many injured?
• Emergency number 06221-387 7821 from mobile phones.
• Please report all accidents to the conference officer at the registration desk.

In case of fire

• Press the nearest fire alarm button or the Emergency number 222 from any internal phone.
• A loud fire alarm will go off if an evacuation is required. On hearing the alarm leave the building immediately following the escape route and fire wardens and head to one of the meeting points
• Do not use the lifts.

Beyond first aid

The Heidelberg University Hospital or ‘Universitätsklinikum‘ has a 24 hour accident and emergency facility and is at ‘Im Neuenheimer Feld 672’. It is a 8km or a 20 minute taxi ride away from EMBL. In a medical emergency, the ambulance service generally transports patients to this hospital.

Please remember to bring your own medication, if needed, to the conference. Note that the next pharmacy is a 4-minute drive from the EMBL, but for many medications you will be required to see a doctor to get a prescription.

Ensure in advance that your medical insurance will cover you during your visit in the event that you do need to see a doctor while in Heidelberg.

The EMBL course and conference office team are able to advise you on where to turn for emergency or non-emergency medical treatment in Heidelberg and can assist you in making appointments and / or arranging transport to get to the pharmacy or a doctor of your choice if necessary. Please note, we are not however permitted to personally accompany you off-campus.

Sightseeing

A variety of activities in Heidelberg can be found on the website of Heidelberg Marketing.

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Technical requirements

Below you will find the software and technical requirements for this course. You will need admin rights to be able to install the software on your computer and we recommend you try this before the course. If you do not have admin rights, please contact your technical support team in advance to make sure the software can be installed.

Additional software

The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus. 

Zoom will be used for the live talks and Q&A sessions. More information will be provided closer to the start of the course.

Other information

While using the software during the course, please make sure you have nothing else using your bandwidth. We recommend using a wired connection.

Please find additional information including FAQs and terms and conditions on our Information for Participants page.