Edit
The fundamentals of high-end cell sorting – Course and Conference Office

EMBO Practical Course

The fundamentals of high-end cell sorting

Overview

Proof of COVID-19 vaccination or recovery is required to attend this on-site course. Please see EMBL’s COVID-19 safety policy for on-site events.

Course overview

This course includes a robust theoretical part on the fundamentals of cell sorting delivered by world-leading experts and practical sessions on using standard cuvette sorters. In addition, the course program will include principles, applications, and hands-on sessions on Image Cell Sorting (ICS), allowing attendees to get in touch with this exciting technology!

Audience

The course is intended for Ph.D. students and postdocs who are starting to use or already using cell sorting in their research projects. Applicants with previous active knowledge of basic principles of flow cytometry and regular use of flow cytometers in daily research will profit most from the course content.

Modules/resources

The topics that will be covered include; instrument set-up and validation, correct operation to avoid technical insufficiency, sample quality, the essentials of gating, performing single-cell sorts for sequencing,  and sorting based on morphological traits. 

The course will provide knowledge transfer from EMBLs Gene Core regarding single-cell genomics. Our main goal is to provide our attendees with the proper knowledge and experience about cell sorting so that they can go back to their research with high confidence about their sorts.

Learning outcomes

The principle objectives are to deliver the theory of cell sorting and the practical sides of this high-end technology.

This course is kindly supported by The Flow Cytometry Core Facility at EMBL Heidelberg.

Speakers

Speakers, trainers, and staff

Derek Davies

Derek Davies

The Francis Crick Institute

UK

Gelo dela Cruz

Gelo dela Cruz

Novo Nordisk Foundation Center for Stem Cell Medicine – reNEW

Denmark

Aaron Middlebrook

BD Biosciences

USA

Malte Paulsen

Malte Paulsen

Novo Nordisk Foundation Center for Stem Cell Medicine – reNEW

Denmark

Steffen Schmitt

German Cancer Research Center (DKFZ)

Germany

Scientific Organisers

Derek Davies

Derek Davies

The Francis Crick Institute

UK

Malte Paulsen

Malte Paulsen

Novo Nordisk Foundation Center for Stem Cell Medicine – reNEW

Denmark

Steffen Schmitt

German Cancer Research Center (DKFZ)

Germany

Course Organisers

Maria Mercedes Bacadare Goitia

Course and Conference Manager

EMBL Heidelberg

Germany

Yvonne Yeboah

Yvonne Yeboah

Training Laboratory Manager

EMBL Heidelberg

Germany

Programme

Got something to say? Tweet it! #EMBOCellSort

  • Some pre-course work (such as watching pre-recordings, protocol reading and computational exercises) with estimated time 0-10 hours will be required and will be shared in advance of the course.
  • Some speakers may need to join virtually to give their lecture or Q&A, and the programme is subject to change.
Day 1 – Sunday 30 October 2022
Time
(Europe/Belin)
 SpeakerLocation
08:40 – 08:55Bus from hotel to EMBL ATC EntranceISG Hotel
08:55 – 09:10RegistrationFlex Lab A & B
09:10 – 09:55Welcome, house notes and course overview and Safety OverviewFlex Lab A & B
09:55 – 10:30FlashtalksFlex Lab A & B
10:30 – 11:00Coffee breakFlex Lab A & B
11:00 – 11:45Lecture: Fluidics and optics
Gelo de la cruz – Novo Nordisk Foundation Center for Stem Cell Medicine – reNEW, Denmark
Flex Lab A & B
11:45 – 12:30Lecture: Signal Generation
Malte Paulsen – Novo Nordisk Foundation Center for Stem Cell Medicine – reNEW, Denmark
Flex Lab A & B
12:30 – 13:30LunchCanteen
13:30 – 14:15Lecture: Stream, Nozzles & Drops
Andrew Filby – Newcastle University, UK
Flex Lab A & B
14:15 – 15:00Lecture: Drop Delay
Joana Cerveira – Cambridge University, UK
Flex Lab A & B
15:00 – 16:30Poster session 1 (odd numbers) and coffee break
16:30 – 18:30Practical session
Group 1: Drop delay and Rmax
Group 2: Laser alignment
All Instructors
Training Lab B
18:30 – 18:45Departure to HotelEMBL Bus Stop / ATC Entrance
18:45 – 19:45DinnerISG Hotel
Day 2 – Monday 31 October 2022
Time (Europe/Belin)SpeakerLocation
08:40 – 08:55Bus from hotel to EMBL ATC EntranceISG Hotel
08:55 – 09:05Daily check-in
09:05 – 09:15Overview of the dayFlex Lab A & B
09:15 – 10:00Lecture: Cell Sorting Applications
Michael Bonadonna – EMBL Heidelberg, Germany
Flex Lab A & B
10:00 – 10:45Lecture: Sample Quality Impact
Christopher Hall – Babraham Institute Cambridge University, UK
Flex Lab A & B
10:45 – 11:15BreakFlex Lab A & B
11:15 – 12:30Lecture: The essentials of gating, FMO and gating controls
Beata Ramasz – EMBL Heidelberg, Germany
Flex Lab A & B
12:30 – 13:30LunchEMBL Canteen
13:30 – 14:45Lecture: Cell Sorting Systems
Diana Ordonez – EMBL Heidelberg, Germany
Flex Lab A & B
14:45 – 15:30Lecture: Sorting modes and Index cell sorting
Daniel Gimenes – EMBL Heidelberg, Germany
Flex Lab A & B
15:30 – 16:15Poster session 2 (even numbers) and coffee break
16:15 – 18:30Practical session
Group 1: Drop delay and Rmax
Group 2: Laser alignment
All Instructors
Training Lab B
18:30 – 19:30DinnerEMBL Canteen
19:30 – 19:45Departure to HotelEMBL Bus Stop / ATC Entrance
Day 3 – Tuesday 1 November 2022
Time (Europe/Belin)SpeakerLocation
08:40 – 08:55Bus from hotel to EMBL ATC EntranceISG Hotel
08:55 – 09:05Daily check-in
09:05 – 09:15Overview of the day
09:15 – 10:00Lecture: Image cell sorting
BD Biosciences
Flex Lab A & B
10:00 – 10:45Coffee break and transfers to DKFZ
10:45 – 12:30Practical Session: Running Different Sorter Types
Group 1: Image cell sorter (EMBL)
Group 2: Aria Fusion (EMBL)
Group 3: Aria Fusion (DKFZ)
Training Lab B
12:30 – 13:30Group LunchEMBL/DKFZ Canteen
13:30 – 18:30Practical Session: Running Different Sorter Types
Group 1: Image cell sorter (EMBL)
Group 2: Aria Fusion (EMBL)
Group 3: Aria Fusion (DKFZ)
Training Lab B
18:30Free evening
18:30 – 18:45Departure to DowntownEMBL Bus Stop / ATC Entrance
Day 4 – Wednesday 2 November 2022
Time (Europe/Belin)SpeakerLocation
08:40 – 08:55Bus from hotel to EMBL ATC EntranceISG Hotel
08:55 – 09:05Daily check-in
09:05 – 09:15Overview of the dayFlex Lab A & B
09:15 – 10:00Lecture: Quality Single Cell Sorts
Cora Chadick – Amsterdam UMC, location VUmc, The Netherlands
Flex Lab A & B
10:00 – 10:45Lecture: Single Cell Genomics
Vladimir Benes – EMBL Heidelberg
Flex Lab A & B
10:45 – 11:15Coffee break and transfers to DKFZFlex Lab A & B
11:15 – 13:00Practical Session: Running Different Sorter Types
Group 1: Aria Fusion (DKFZ)
Group 2: Image cell sorter (EMBL)
Group 3: Aria Fusion (EMBL)
Training Lab B
13:00 – 14:00Group LunchEMBL / DKFZ Canteen
14:00 – 19:00Practical Session: Running Different Sorter Types
Group 1: Aria Fusion (DKFZ)
Group 2: Image cell sorter (EMBL)
Group 3: Aria Fusion (EMBL)
Training Lab B
19:00Dinner and networking downtown
19:00 – 19:15Departure to DowntownEMBL Bus Stop / ATC Entrance
Day 5 – Thursday 3 November 2022
Time (Europe/Belin)SpeakerLocation
08:40 – 08:55Bus from hotel to EMBL ATC EntranceISG Hotel
08:55 – 09:05Daily check-in
09:05 – 09:15Overview of the dayFlex Lab A & B
09:15 – 10:15Lecture: Biosafety Considerations
Steffen Schmitt – German Cancer Research Center (DKFZ), Germany
Flex Lab A & B
10:15 – 10:45Coffee break and transfers to DKFZFlex Lab A & B
10:45 – 12:30Practical Session: Running Different Sorter Types
Group 1: Aria Fusion (EMBL)
Group 2: Aria Fusion (DKFZ)
Group 3: Image cell sorter (EMBL)
Training Lab B
12:30 – 13:30Group LunchEMBL / DKFZ Canteen
13:30 – 18:30Practical Session: Running Different Sorter Types
Group 1: Aria Fusion (EMBL)
Group 2: Aria Fusion (DKFZ)
Group 3: Image cell sorter (EMBL)
Training Lab B
18:30Dinner and networking downtown
18:30 – 18:45Departure to DowntownEMBL Bus Stop / ATC Entrance
Day 6 – Friday 4 November 2022
Time (Europe/Belin)SpeakerLocation
08:40 – 08:55Bus from hotel to EMBL ATC EntranceISG Hotel
08:55 – 09:05Daily check-in
09:05 – 09:15Overview of the dayFlex Lab A & B
09:15 – 10:15Lecture: Tips for publishing high quality cytometry data
Derek Davies – The Francis Crick Institute, UK
Flex Lab A & B
10:15 – 10:45Coffee breakFlex Lab A & B
10:45 – 12:30Genomic centre tour & single cell genomics workflow
GeneCore Facility Team
GeneCore
12:30 – 13:00Feedback and discussionFlex Lab A & B
13:00 – 13:15Packed lunches and departure Flex Lab A & B
13:15 – 13:15Departure to Heidelberg Train Station and DowntownEMBL Bus Stop / ATC Entrance

Practical information

Registration fees and motivation letter

The course is limited to 12 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.

Registration

Registration fees include admission, course materials, COVID-19 safety measures, meals and coffee breaks.

This EMBO course includes accommodation and transportation to and from the ISG Hotel to the venue.

Academia€400
PhD Student€400
Industry€1000

NO visa support letters will be issued until payment of the registration fee is confirmed.

Confirmation and payment

The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.

Motivation letter submission

After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.

Instructions

  1. Include your relevant skills, experience and qualifications showing why you would be suitable for the course.
  2. Explain why you would like to attend, including what you can contribute and how you think you will benefit from the course.

Please note:

  • The limit of 2000 characters refers to manually typed text and excludes spaces. If an error occurs try using a different web browser (preferably Google Chrome or Mozilla Firefox).
  • If you copy-paste the text into the form, hidden formatting might still be included which may cause the text to exceed the 2,000 character limit resulting in an error message. We recommend you clear all formatting before pasting in the text.
  • If you have special symbols in your text, make sure you are using Unicode characters, otherwise these will not be recognised.

For detailed instructions, please watch our video on how to submit a course motivation letter.

Further details

For further information about registration and motivation letter submission please refer to the FAQ page.

Financial assistance

Financial assistance

Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of both registration fee waivers and travel grants. Availability is limited to participants attending on-site events in Heidelberg and will be indicated during the abstract or motivation letter submission process.

Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.

Registration fee waiver

The fee waiver will cover the registration sum that you have paid to attend the course or conference.

Travel grant

The travel grant will cover the cost of travel (airfare, train, bus, taxi, accommodation, visa, and/or registration fees*) and is provided up to specified caps which are normally as follows:

– up to €400 for participants travelling to an EMBL Course, EMBL Conference or EMBO|EMBL Symposium from within Europe.
– up to €1000 for participants travelling to an EMBL Course, EMBL Conference or EMBO|EMBL Symposium from outside Europe.
– up to €500 for any participant travelling to an EMBO Practical Course or EMBO Workshop.
– up to €1000 for any participant working in Chile, India, Singapore or Taiwan travelling to an EMBO Practical Course or EMBO Workshop.

*Registration fees are only covered for EMBO Practical Courses or EMBO Workshops

The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.

Childcare grant (EMBO Practical Courses and Workshops only)

For EMBO Practical Course or EMBO Workshop participants with children, there is the possibility to apply for a childcare grant to offset child care costs incurred by participants or speakers when participating at a course or conference. Eligible costs include fees for a babysitter or child-care facility, travel costs for a caregiver, or travel costs for taking the child to the meeting etc. Please note that priority will be given to early-stage researchers. A maximum amount of 500 EUR can be awarded per participant applying for an EMBO Childcare Grant. In order to apply for this grant for EMBO Workshops, you must be registered by the abstract submission deadline.

Boehringer Ingelheim Fonds travel grant

Applies to selected courses only. Availability will be indicated during the abstract or motivation letter submission process.

This grant covers costs related to your attendance at the course (registration, travel and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.
Whether you are eligible to apply for a travel grant, depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):

– for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course
– for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course

Application

You may apply for financial assistance when submitting your motivation letter for courses, and abstract for conferences. In your application, you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.

For the Boehringer Ingelheim Fonds Travel Grant, there is a pre-application question during the motivation letter submission process, and if selected you will be requested to complete a standard form and documentation consisting of your travel expense estimation.

Selection

The scientific organisers will select the recipients of all financial assistance during the motivation letter or abstract selection process. Results will be announced approximately 6-8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the meeting. Selection is based on your current work or study location, the reasons for needing financial support and the impact this event will have on your career.

Reimbursement

Costs will be reimbursed after the meeting only once a reimbursement form and original receipts (from travel costs) have been received.

Further details

See our list of external funding opportunities and information on attending a conference as an event reporter.

For further information about financial assistance please refer to the FAQ page.

Accommodation and shuttles

Hotel

The registration fee includes accommodation for 6 nights (29 Oct – 4 Nov) at the ISG Hotel in a single room, including breakfast. Any additional nights need to be booked and paid for by you to the hotel. We recommend that all course participants stay at the ISG Hotel, which is the dedicated hotel for the course.

Local transport

Shuttle buses will go from the ISG Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.

Further details

Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

For further information on getting to EMBL Heidelberg visit our Travel Information page.

For enquiries about accommodation and local transportation please refer to the FAQ page.

Event software

Event software

The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus. 

Additional information

Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.

COVID-19 related questions

COVID-19 information for on-site events at EMBL Heidelberg can be found in our COVID-19 FAQs.

Sponsors

Event Supporter:

Sponsorship opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other conferences, or contact sponsorship@embl.de for further information.

If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.

Date: 30 Oct - 4 Nov 2022

Location: EMBL Heidelberg


Deadline(s):

Application: Closed


Organisers:

  • Derek Davis
    The Francis Crick Institute, UK
    • Andrew Filby
      Newcastle University, UK
      • Diana Ordoñez
        EMBL Heidelberg, Germany
        • Malte Paulsen
          Novo Nordisk Foundation Center for Stem Cell Medicine – reNEW, Denmark
          • Steffen Schmitt
            German Cancer Research Center (DKFZ), Germany

          Contact: Maria Bacadare

          Download event poster


EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme

Founder partners

Corporate partners

Associate partners

Edit