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Scientists from EMBL Heidelberg and the Institute of Molecular Biotechnology have uncovered how cells remove unwanted components from the nucleus following mitosis.
The organisation of cells into specific compartments is critical for their function. For instance, by separating the nucleus from the cytoplasm, the nuclear envelope prevents premature translation of immature RNAs.
During mitosis, however, the nuclear envelope disassembles, allowing large cytoplasmic components such as ribosomes to mix with nuclear material. When the nuclear envelope reassembles following mitosis, these cytoplasmic components must once again be removed. “The nuclear envelope can contribute to this by actively importing or exporting substrates up to a certain size, but it was not clear what happens with very large cytoplasmic components”, says Mina Petrovic, from the Gerlich lab at IMBA – the Institute of Molecular Biotechnology – and joint first author of the study.
The research team from IMBA and EMBL have now shown that large components such as ribosomes are in fact removed from the forming nucleus before the nuclear envelope is assembled again. This exclusion process requires the protein Ki-67, which was the focus of an earlier publication in Nature by Sara Cuylen-Häring, group leader at EMBL Heidelberg and the other joint first author of this study.
In this older study it was discovered that Ki-67 was responsible for keeping chromosomes separated in early stages of mitosis by acting as a surfactant. “Remarkably, we have now found that it changes its properties at the end of mitosis and performs the opposite function, namely clustering of chromosomes,” said Cuylen-Häring. By coming together into a dense cluster at the end of cell division, chromosomes are able to exclude large cytoplasmic components before the nuclear envelope reforms.
“This collaborative work shows how a single protein can dynamically change the surface properties of chromosomes,” explains Alberto Hernandez Armendariz, PhD student in the Cuylen-Häring group. “Ultimately, this facilitates effective compartmentalisation of key processes within the cell.”
Sara Cuylen et al. Ki-67 acts as a biological surfactant to disperse mitotic chromosomes. Nature, published 29 June 2016. DOI: 10.1038/nature18610
Sara Cuylen-Häring et al. Chromosome clustering by Ki-67 excludes cytoplasm during nuclear assembly. Nature, published on 02 September 2020. DOI: 10.1038/s41586-020-2672-3
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