Publications

Triangular in vivo self-assembling coiled-coil protein origami.

Božič Abram S, Gradišar H, Aupič J, Round AR, Jerala R

ACS chemical biology 2021

33476117. doi:10.1021/acschembio.0c00812.

Statistically correcting dynamical electron scattering improves the refinement of protein nanocrystals, including charge refinement of coordinated metals.

Blum TB, Housset D, Clabbers MTB, van Genderen E, Bacia-Verloop M, Zander U, McCarthy AA, Schoehn G, Ling WL, Abrahams JP

Acta crystallographica. Section D, Structural biology 2021

33404527. doi:10.1107/S2059798320014540.

Structural basis of client specificity in mitochondrial membrane-protein chaperones.

Sučec I, Wang Y, Dakhlaoui O, Weinhäupl K, Jores T, Costa D, Hessel A, Brennich M, Rapaport D, Lindorff-Larsen K, Bersch B, Schanda P

Science advances 2020

33355130. doi:10.1126/sciadv.abd0263.

Low-dose in situ prelocation of protein microcrystals by 2D X-ray phase-contrast imaging for serial crystallography.

Martiel I, Huang CY, Villanueva-Perez P, Panepucci E, Basu S, Caffrey M, Pedrini B, Bunk O, Stampanoni M, Wang M

IUCrJ 2020

33209324. doi:10.1107/S2052252520013238.

Attaining atomic resolution from in situ data collection at room temperature using counter-diffusion-based low-cost microchips.

Gavira JA, Rodriguez-Ruiz I, Martinez-Rodriguez S, Basu S, Teychené S, McCarthy AA, Mueller-Dieckman C

Acta crystallographica. Section D, Structural biology 2020

32744257. doi:10.1107/S2059798320008475.

X-ray structure of the human karyopherin RanBP5, an essential factor for influenza polymerase nuclear trafficking.

Swale C, Da Costa B, Sedano L, Garzoni F, McCarthy AA, Berger I, Bieniossek C, Ruigrok RWH, Delmas B, Crépin T

Journal of molecular biology 2020

32222384. doi:10.1016/j.jmb.2020.03.021.

XFEL and NMR Structures of Francisella Lipoprotein Reveal Conformational Space of Drug Target against Tularemia.

Zook J, Shekhar M, Hansen D, Conrad C, Grant T, Gupta C, White T, Barty A, , Zhao Y, Zatsepin N, Ishchenko A, Batyuk A, Gati C, Li C, Coe J, Galli L, Liang M, Hunter M, Nelson G, Weierstall U, Stauch B, James D, Thifault D, Craciunescu F, Cherezov V, Liu W, Fromme P, Singharoy A

Structure (London, England : 1993) 2020

32142641. doi:10.1016/j.str.2020.02.005.

Structural and functional comparison of fumarylacetoacetate domain containing protein 1 (FAHD1) in human and mouse.

Weiss AKH, Naschberger A, Cappuccio E, Metzger C, Mottes L, Holzknecht M, von Velsen J, Bowler MW, Rupp B, Jansen-Dürr P

Bioscience reports 2020

32068790. doi:10.1042/BSR20194431.

Structure and function of roundabout receptors.

Bisiak F, McCarthy AA

Sub-cellular biochemistry 2019

31939155. doi:10.1007/978-3-030-28151-9_9.

Deciphering copper coordination in the mammalian prion protein amyloidogenic domain.

Salzano G, Brennich M, Mancini G, Tran TH, Legname G, D'Angelo P, Giachin G

Biophysical journal 2020

31952810. doi:10.1016/j.bpj.2019.12.025.

Decapping enzyme NUDT12 partners with BLMH for cytoplasmic surveillance of NAD-capped RNAs.

Wu H, Li L, Chen KM, Homolka D, Gos P, Fleury-Olela F, McCarthy AA, Pillai RS

Cell reports 2019

31875550. doi:10.1016/j.celrep.2019.11.108.

Controlled dehydration, structural flexibility and gadolinium MRI contrast compound binding in the human plasma glycoprotein afamin.

Naschberger A, Juyoux P, von Velsen J, Rupp B, Bowler MW

Acta crystallographica. Section D, Structural biology 2019

31793901. doi:10.1107/S2059798319013500.

A comparative anatomy of protein crystals: lessons from the automatic processing of 56 000 samples.

Svensson O, Gilski M, Nurizzo D, Bowler MW

IUCRJ 2019

31576216. doi:10.1107/S2052252519008017.

Lipid-based quaternary ammonium sophorolipid amphiphiles with antimicrobial and transfection activities.

Delbeke EIP, Everaert J, Lozach O, Le Gall T, Berchel M, Montier T, Jaffrès PA, Rigole P, Coenye T, Brennich M, Baccile N, Roelants SLKW, Soetaert W, Van Bogaert INA, Van Geem KM, Stevens CV

ChemSusChem 2019

31081279. doi:10.1002/cssc.201900721.

High-throughput production of influenza virus-like particle (VLP) array by using VLP-factory™, a MultiBac baculoviral genome customized for enveloped VLP expression.

Sari-Ak D, Bahrami S, Laska MJ, Drncova P, Fitzgerald DJ, Schaffitzel C, Garzoni F, Berger I

Methods in molecular biology (Clifton, N.J.) 2019

31267455. doi:10.1007/978-1-4939-9624-7_10.

Small-angle neutron and X-ray scattering analysis of the supramolecular organization of rhodopsin in photoreceptor membranes.

Feldman TB, Ivankov OI, Kuklin AI, Murugova TN, Yakovleva MA, Smitienko OA, Kolchugina IB, Round A, Gordeliy VI, Belushkin AV, Ostrovsky MA

BBA - Biomembranes 2019

31152688. doi:10.1016/j.bbamem.2019.05.022.

Structure of the human TRPML2 ion channel extracytosolic/lumenal domain.

Viet KK, Wagner A, Schwickert K, Hellwig N, Brennich M, Bader N, Schirmeister T, Morgner N, Schindelin H, Hellmich UA

Structure (London, England : 1993) 2019

31178222. doi:10.1016/j.str.2019.04.016.

Destabilization of the human RED-SMU1 splicing complex as a basis for host-directed antiinfluenza strategy.

Ashraf U, Tengo L, Le Corre L, Fournier G, Busca P, McCarthy AA, Rameix-Welti MA, Gravier-Pelletier C, Ruigrok RWH, Jacob Y, Vidalain PO, Pietrancosta N, Crépin T, Naffakh N

Proceedings of the National Academy of Sciences of the United States of America 2019

31076555. doi:10.1073/pnas.1901214116.

Asymmetrical, symmetrical, divalent, and Y-shaped (bola)amphiphiles: the relationship between the molecular structure and self-assembly in amino derivatives of sophorolipid biosurfactants.

Baccile N, Delbeke EIP, Brennich M, Seyrig C, Everaert J, Roelants SLKW, Soetaert W, Van Bogaert INA, Van Geem KM, Stevens CV

The journal of physical chemistry. B 2019

31002250. doi:10.1021/acs.jpcb.9b01013.

Fully autonomous characterization and data collection from crystals of biological macromolecules.

Hutin S, Van Laer B, Mueller-Dieckmann C, Leonard G, Nurizzo D, Bowler MW

Journal of visualized experiments : JoVE 2019

30958484. doi:10.3791/59032.

Structural principles in Robo activation and auto-inhibition.

Barak R, Yom-Tov G, Guez-Haddad J, Gasri-Plotnitsky L, Maimon R, Cohen-Berkman M, McCarthy AA, Perlson E, Henis-Korenblit S, Isupov MN, Opatowsky Y

Cell 2019

30853216. doi:10.1016/j.cell.2019.02.004.

Structure solution of the fluorescent protein cerulean using MeshAndCollect.

Hutin S, Santoni G, Zander U, Foos N, Aumonier S, Gotthard G, Royant A, Mueller-Dieckmann C, Leonard G

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS 2019

30958477. doi:10.3791/58594.

MXCuBE2: the dawn of MXCuBE Collaboration.

Oscarsson M, Beteva A, Flot D, Gordon E, Guijarro M, Leonard G, McSweeney S, Monaco S, Mueller-Dieckmann C, Nanao M, Nurizzo D, Popov AN, von Stetten D, Svensson O, Rey-Bakaikoa V, Chavas LMG, Chado I, Gourhant P, Gadea L, Legrand P, Isabet T, Sirigu S, Savko M, Thompson A, Shepard W, Nan J, Mueller U, Bolmsten F, Eguiraun M, Milàn-Otero A, Nardella A, Kastner A, Schmuckermaier L, Thunnissen M, Hellmig M, Weiss MS, Gerlach M, Feiler C, Bowler MW, Gobbo A, Papp G, Sinoir J, McCarthy AA, Karpics I, Nikolova M, Bourenkov G, Schneider T, Boer R, Juanhuix J, Cuní G, Andreu J, Paciorek W, Flensburg C, Keller P, Fogh R, de Sanctis D, Bricogne G, Vonrhein C

Journal of synchrotron radiation 2019

30855248. doi:10.1107/S1600577519001267.

MultiBac: baculovirus-mediated multigene DNA cargo delivery in insect and mammalian cells.

Gupta K, Tölzer C, Sari-Ak D, Fitzgerald DJ, Schaffitzel C, Berger I

Viruses 2019

30813511. doi:10.3390/v11030198.

A molecular mechanism for transthyretin amyloidogenesis.

Yee AW, Aldeghi M, Blakeley MP, Ostermann A, Mas PJ, Moulin M, de Sanctis D, Bowler MW, Mueller-Dieckmann C, Mitchell EP, Haertlein M, de Groot BL, Boeri Erba E & Forsyth VT

Nature Communications 2019

30804345. doi:10.1038/s41467-019-08609-z.

Long-wavelength Mesh&Collect native SAD phasing from microcrystals.

Cianci M, Nanao M, Schneider TR

Acta crystallographica. Section D, Structural biology 2019

30821707. doi:10.1107/S2059798319002031.

Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes.

Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA

Angewandte Chemie (International ed. in English) 2019

30605929. doi:10.1002/anie.201810470.

CrystalDirect-To-Beam: Opening the shortest path from crystal to data.

Felisaz F, Sinoir J, Papp G, Pica A, Bowler MW, Murphy P, Hoffmann G, Zander U, Lopez-Marrero M, Janocha R, Giraud T, Svensson O, Popov S, Leonard G, Mueller-Dieckmann C, Márquez JA, McCarthy A, Cipriani F

2019

doi:10.1063/1.5084627.

Structural basis for the activation of the deubiquitinase Calypso by the Polycomb protein ASX.

De I, Chittock EC, Grötsch H, Miller TCR, McCarthy AA, Müller CW

Structure (London, England : 1993) 2019

30639226. doi:10.1016/j.str.2018.11.013.

Structural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space.

Weinhäupl K, Lindau C, Hessel A, Wang Y, Schütze C, Jores T, Melchionda L, Schönfisch B, Kalbacher H, Bersch B, Rapaport D, Brennich M, Lindorff-Larsen K, Wiedemann N, Schanda P

Cell 2018

30445040. doi:10.1016/j.cell.2018.10.039.

Structural basis for the bi-functionality of human oxaloacetate decarboxylase FAHD1.

Weiss AKH, Naschberger A, Loeffler JR, Gstach H, Bowler MW, Holzknecht M, Cappuccio E, Pittl A, Etemad S, Dunzendorfer-Matt T, Scheffzek K, Liedl KR, Jansen-Dürr P

The Biochemical journal 2018

30348641. doi:10.1042/BCJ20180750.

Bio-based glyco-bolaamphiphile forms a temperature-responsive hydrogel with tunable elastic properties.

Baccile N, Van Renterghem L, Le Griel P, Ducouret G, Brennich M, Cristiglio V, Roelants SLKW, Soetaert W

Soft matter 2018

30211424. doi:10.1039/c8sm01167b.

Methylation of Structured RNA by the m6A Writer METTL16 Is Essential for Mouse Embryonic Development.

Mendel M, Chen KM, Homolka D, Gos P, Pandey RR, McCarthy AA, Pillai RS

Molecular cell 2018

30197299. doi:10.1016/j.molcel.2018.08.004.

Calibration of rotation axes for multi-axis goniometers in macromolecular crystallography.

White KI, Bugris V, McCarthy AA, Ravelli RBG, Csankó K, Cassetta A, Brockhauser S

Journal of applied crystallography 2018

30279641. doi:10.1107/S1600576718010956.

Structural basis for Scc3-dependent cohesin recruitment to chromatin.

Li Y, Muir K, Bowler MW, Metz J, Haering CH, Panne D

eLife 2018

30109982. doi:10.7554/eLife.38356.

Structural basis for reactivating the mutant TERT promoter by cooperative binding of p52 and ETS1.

Xu X, Li Y, Bharath SR, Ozturk MB, Bowler MW, Loo BZL, Tergaonkar V, Song H

Nature communications 2018

30093619. doi:10.1038/s41467-018-05644-0.

van der Waals Contact between Nucleophile and Transferring Phosphorus Is Insufficient To Achieve Enzyme Transition-State Architecture

Johnson LA, Robertson AJ, Baxter NJ, Trevitt CR, Bisson C, Jin Y, Wood HP, Hounslow AM, Cliff MJ, Blackburn GM, Bowler MW, Waltho JP

ACS Catalysis 2018

doi:10.1021/acscatal.8b01612.

Complex coacervation of natural sophorolipid bolaamphiphile micelles with cationic polyelectrolytes

Ben Messaoud G, Promeneur L, Brennich M, Roelants SLKW, Le Griel P, Baccile N

GREEN CHEMISTRY 2018

doi:10.1039/c8gc01531g.

Synthesis and Biological Evaluation of Bolaamphiphilic Sophorolipids

Delbeke EIP, Everaert J, Lozach O, Le Gall T, Berchel M, Montier T, Jaffres PA, Rigole P, Coenye T, Brennich M, Baccile N, Roelants SLKW, Soetaert W, van Bogaert INA, Van Geem KM, Stevens CV

ACS SUSTAINABLE CHEMISTRY & ENGINEERING 2018

doi:10.1021/acssuschemeng.8b01354.

ID30B - a versatile beamline for macromolecular crystallography experiments at the ESRF.

McCarthy AA, Barrett R, Beteva A, Caserotto H, Dobias F, Felisaz F, Giraud T, Guijarro M, Janocha R, Khadrouche A, Lentini M, Leonard GA, Lopez Marrero M, Malbet-Monaco S, McSweeney S, Papp G, Nurizzo D, Sinoir J, Rossi C, Surr J, Sorez C, Zander U, Svensson O, Theveneau P, Cipriani F, Mueller-Dieckmann C

Journal of synchrotron radiation 2018

29979188. doi:10.1107/S1600577518007166.

Smaller capillaries improve the small-angle X-ray scattering signal and sample consumption for biomacromolecular solutions.

Schroer MA, Blanchet CE, Gruzinov AY, Gräwert MA, Brennich ME, Hajizadeh NR, Jeffries CM, Svergun DI

2018

29979172. doi:10.1107/S1600577518007907.

A Novel Amyloid Designable Scaffold and Potential Inhibitor Inspired by GAIIG of Amyloid Beta and the HIV-1 V3 loop.

Kokotidou C, Jonnalagadda SVR, Orr AA, Seoane-Blanco M, Apostolidou CP, van Raaij MJ, Kotzabasaki M, Chatzoudis A, Jakubowski JM, Mossou E, Forsyth VT, Mitchell EP, Bowler MW, Llamas-Saiz AL, Tamamis P, Mitraki A

FEBS letters 2018

29772603. doi:10.1002/1873-3468.13096.

The antibiotic cyclomarin blocks arginine-phosphate-induced millisecond dynamics in the N-terminal domain of ClpC1 from Mycobacterium tuberculosis.

Weinhäupl K, Brennich M, Kazmaier U, Lelievre J, Ballell L, Goldberg A, Schanda P, Fraga H

The Journal of biological chemistry 2018

29632076. doi:10.1074/jbc.RA118.002251.

Author Correction: Structural analysis of human ARS2 as a platform for co-transcriptional RNA sorting.

Schulze WM, Stein F, Rettel M, Nanao M, Cusack S

Nature communications 2018

29855469. doi:10.1038/s41467-018-04643-5.

Multi-position data collection and dynamic beam sizing: recent improvements to the automatic data-collection algorithms on MASSIF-1

Svensson S, Gilski M, Nurizzo D, Bowler MW

2018

29717714. doi:10.1107/S2059798318003728.

Structural analysis of human ARS2 as a platform for co-transcriptional RNA sorting.

Schulze WM, Stein F, Rettel M, Nanao M, Cusack S

Nature communications 2018

29703953. doi:10.1038/s41467-018-04142-7.

Vesicle Adhesion and Fusion Studied by Small-Angle X-Ray Scattering.

Komorowski K, Salditt A, Xu Y, Yavuz H, Brennich M, Jahn R, Salditt T

Biophysical journal 2018

29694868. doi:10.1016/j.bpj.2018.02.040.

Insc:LGN tetramers promote asymmetric divisions of mammary stem cells.

Culurgioni S, Mari S, Bonetti P, Gallini S, Bonetto G, Brennich M, Round A, Nicassio F, Mapelli M

Nature communications 2018

29523789. doi:10.1038/s41467-018-03343-4.

Dynamic self-assembly of DNA minor groove-binding ligand DB921 into nanotubes triggered by an alkali halide.

Mizuta R, Devos JM, Webster J, Ling WL, Narayanan T, Round A, Munnur D, Mossou E, Farahat AA, Boykin DW, Wilson WD, Neidle S, Schweins R, Rannou P, Haertlein M, Forsyth VT, Mitchell EP

Nanoscale 2018

29517086. doi:10.1039/c7nr03875e.

Robo1 Forms a Compact Dimer-of-Dimers Assembly.

Aleksandrova N, Gutsche I, Kandiah E, Avilov SV, Petoukhov MV, Seiradake E, McCarthy AA

Structure (London, England : 1993) 2018

29307485. doi:10.1016/j.str.2017.12.003.

A new MR-SAD algorithm for the automatic building of protein models from low-resolution X-ray data and a poor starting model

Skubak P, Arac D, Bowler MW, Correia A, Hoelz A, Larsen S, Leonard G, McCarthy A, McSweeney S, Mueller-Dieckmann C, Otten H, Salzman G, Pannu N

IUCrJ 2018

29765606. doi:10.1107/S2052252517017961.

How to Analyze and Present SAS Data for Publication.

Brennich M, Pernot P, Round A

Advances in experimental medicine and biology 2017

29218553. doi:10.1007/978-981-10-6038-0_4.

Structural Evidence for a Role of the Multi-functional Human Glycoprotein Afamin in Wnt Transport.

Naschberger A, Orry A, Lechner S, Bowler MW, Nurizzo D, Novokmet M, Keller MA, Oemer G, Seppi D, Haslbeck M, Pansi K, Dieplinger H, Rupp B

Structure (London, England : 1993) 2017

29153507. doi:10.1016/j.str.2017.10.006.

Hierarchical clustering for multiple-crystal macromolecular crystallography experiments: the ccCluster program.

Santoni G, Zander U, Mueller-Dieckmann C, Leonard G, Popov A

Journal of applied crystallography 2017

29217993. doi:10.1107/S1600576717015229.

Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release.

Leloup N, Lössl P, Meijer DH, Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC

Nature communications 2017

29167428. doi:10.1038/s41467-017-01485-5.

Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID.

Gupta K, Watson AA, Baptista T, Scheer E, Chambers AL, Koehler C, Zou J, Obong-Ebong I, Kandiah E, Temblador A, Round A, Forest E, Man P, Bieniossek C, Laue ED, Lemke EA, Rappsilber J, Robinson CV, Devys D, Tora L, Berger I

eLife 2017

29111974. doi:10.7554/eLife.30395.

Structural basis for the shielding function of the dynamic trypanosome variant surface glycoprotein coat.

Bartossek T, Jones NG, Schäfer C, Cvitković M, Glogger M, Mott HR, Kuper J, Brennich M, Carrington M, Smith AS, Fenz S, Kisker C, Engstler M

Nature microbiology 2017

28894098. doi:10.1038/s41564-017-0013-6.

Observing enzyme ternary transition state analogue complexes by 19F NMR spectroscopy

Ampaw A, Carroll M, von Velsen J, Bhattasali D, Cohen A, Bowler MW, Jakeman DL

Chemical Science; Chem. Sci. 2017

29619190. doi:10.1039/C7SC04204C.

FlexED8: the first member of a fast and flexible sample-changer family for macromolecular crystallography

Papp G, Felisaz F, Sorez C, Lopez-Marrero M, Janocha R, Manjasetty B,Gobbo A, Belrhali H , Bowler MW, Cirpriani F

2017

28994413. doi:10.1107/S2059798317013596.

Towards a compact and precise sample holder for macromolecular crystallography.

Papp G, Rossi C, Janocha R, Sorez C, Lopez-Marrero M, Astruc A, McCarthy A, Belrhali H, Bowler MW, Cipriani F

Acta crystallographica. Section D, Structural biology 2017

28994412. doi:10.1107/S2059798317013742.

Assessing the Influence of Mutation on GTPase Transition States using X-ray, 19F NMR, and DFT Approaches.

Jin Y, Molt RW, Pellegrini E, Cliff MJ, Bowler MW, Richards NGJ, Blackburn GM, Waltho JP

Angewandte Chemie (International ed. in English) 2017

28498638. doi:10.1002/anie.201703074.

Structure of the Arabidopsis TOPLESS corepressor provides insight into the evolution of transcriptional repression.

Martin-Arevalillo R, Nanao MH, Larrieu A, Vinos-Poyo T, Mast D, Galvan-Ampudia C, Brunoud G, Vernoux T, Dumas R, Parcy F

Proceedings of the National Academy of Sciences of the United States of America 2017

28698367. doi:10.1073/pnas.1703054114.

Computational Design of Amyloid Self-Assembling Peptides Bearing Aromatic Residues and the Cell Adhesive Motif Arg-Gly-Asp

Jonnalagadda, S.V.R., Ornithopoulou, E., Orr, A., Mossou, E., Forsyth, T., Mitchell, E.P., Bowler, M.W., Mitraki, A. and Tamamis, P.

2017

doi:10.1039/C7ME00016B.

Bacterial protease uses distinct thermodynamic signatures for substrate recognition.

Bezerra GA, Ohara-Nemoto Y, Cornaciu I, Fedosyuk S, Hoffmann G, Round A, Márquez JA, Nemoto TK, Djinović-Carugo K

Scientific reports 2017

28588213. doi:10.1038/s41598-017-03220-y.

Innovative High-Throughput SAXS Methodologies Based on Photonic Lab-on-a-Chip Sensors: Application to Macromolecular Studies.

Rodríguez-Ruiz I, Radajewski D, Charton S, Phamvan N, Brennich M, Pernot P, Bonneté F, Teychené S

Sensors (Basel, Switzerland) 2017

28574461. doi:10.3390/s17061266.

Raoult's law revisited: accurately predicting equilibrium relative humidity points for humidity control experiments.

Bowler MG, Bowler DR, Bowler MW

Journal of applied crystallography 2017

28381983. doi:10.1107/S1600576717003636.

SAXS structural studies of Dps from Deinococcus radiodurans highlights the conformation of the mobile N-terminal extensions.

Santos SP, Cuypers MG, Round A, Finet S, Narayanan T, Mitchell EP, Romão CV

Journal of molecular biology 2017

28088481. doi:10.1016/j.jmb.2017.01.008.

Targeting Toxoplasma gondii CPSF3 as a new approach to control toxoplasmosis.

Palencia A, Bougdour A, Brenier-Pinchart MP, Touquet B, Bertini RL, Sensi C, Gay G, Vollaire J, Josserand V, Easom E, Freund YR, Pelloux H, Rosenthal PJ, Cusack S, Hakimi MA

EMBO molecular medicine 2017

28148555. doi:10.15252/emmm.201607370.

Coupling High Throughput Microfluidics and Small-Angle X-ray Scattering to Study Protein Crystallization from Solution.

Pham N, Radajewski D, Round A, Brennich M, Pernot P, Biscans B, Bonneté F, Teychené S

Analytical chemistry 2017

28192906. doi:10.1021/acs.analchem.6b03492.

Integral Membrane Proteins Can Be Crystallized Directly from Nanodiscs

Nikolaev M, Round E, Gushchin I, Polovinkin V, Balandin T, Kuzmichev P, Shewhenko V, Borshchevskiy V, Kuklin A, Round A, Bernhard F, Willbold D, Biildt G, Gordeliy V

CRYSTAL GROWTH & DESIGN 2017

doi:10.1021/acs.cgd.6b01631.

Preparation of an 18 F-Labeled Hydrocyanine Dye as a Multimodal Probe for Reactive Oxygen Species.

Al-Karmi S, Albu SA, Vito A, Janzen N, Czorny S, Banevicius L, Nanao M, Zubieta J, Capretta A, Valliant JF

Chemistry (Weinheim an der Bergstrasse, Germany) 2016

27768812. doi:10.1002/chem.201604473.

Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline.

Brennich ME, Round AR, Hutin S

Journal of visualized experiments : JoVE 2017

28117806. doi:10.3791/54861.

Structural Basis for the Subversion of MAP Kinase Signaling by an Intrinsically Disordered Parasite Secreted Agonist.

Pellegrini E, Palencia A, Braun L, Kapp U, Bougdour A, Belrhali H, Bowler MW, Hakimi MA

2016

27889209. doi:10.1016/j.str.2016.10.011.

Binding of Myomesin to Obscurin-Like-1 at the Muscle M-Band Provides a Strategy for Isoform-Specific Mechanical Protection.

Pernigo S, Fukuzawa A, Beedle AE, Holt M, Round A, Pandini A, Garcia-Manyes S, Gautel M, Steiner RA

Structure (London, England : 1993) 2016

27989621. doi:10.1016/j.str.2016.11.015.

Development of tools to automate quantitative analysis of radiation damage in SAXS experiments.

Brooks-Bartlett JC, Batters RA, Bury CS, Lowe ED, Ginn HM, Round A, Garman EF

Journal of synchrotron radiation 2017

28009547. doi:10.1107/s1600577516015083.

Self-assembled amyloid peptides with Arg-Gly-Asp (RGD) motifs as scaffolds for tissue engineering

Deidda G., Jonnalagadda SVR., Spies JW., Ranella A., Mossou E., Forsyth VT., Mitchell EP., Bowler MW., Tamamis P., Mitraki A.

2016

33429698. doi:10.1021/acsbiomaterials.6b00570.

Structural basis for blocking PD-1-mediated immune suppression by therapeutic antibody pembrolizumab.

Na Z, Yeo SP, Bharath SR, Bowler MW, Balıkçı E, Wang CI, Song H

Cell research 2016

27325296. doi:10.1038/cr.2016.77.

Vaccinia Virus Immunomodulator A46: A Lipid and Protein-Binding Scaffold for Sequestering Host TIR-Domain Proteins.

Fedosyuk S, Bezerra GA, Radakovics K, Smith TK, Sammito M, Bobik N, Round A, Ten Eyck LF, Djinović-Carugo K, Usón I, Skern T

PLoS pathogens 2016

27973613. doi:10.1371/journal.ppat.1006079.

Structural basis for specific recognition of pre-snRNA by Gemin5.

Tang X, Bharath SR, Piao S, Tan VQ, Bowler MW, Song H

Cell research 2016

27834343. doi:10.1038/cr.2016.133.

Combined small angle X-ray solution scattering with atomic force microscopy for characterizing radiation damage on biological macromolecules

Costa L, Andriatis A, Brennich M, Teulon JM, Chen SWW, Pellequer JL, Round A

BMC STRUCTURAL BIOLOGY 2016

27788689. doi:10.1186/s12900-016-0068-2.

Online ion-exchange chromatography for small-angle X-ray scattering.

Hutin S, Brennich M, Maillot B, Round A

Acta crystallographica. Section D, Structural biology 2016

27710930. doi:10.1107/S2059798316012833.

Structural basis of host Autophagy-related protein 8 (ATG8) binding by the Irish potato famine pathogen effector protein PexRD54.

Maqbool A, Hughes RK, Dagdas YF, Tregidgo N, Zess E, Belhaj K, Round A, Bozkurt TO, Kamoun S, Banfield MJ

The Journal of biological chemistry 2016

27458016. doi:10.1074/jbc.M116.744995.

Merging of synchrotron serial crystallographic data by a genetic algorithm.

Zander U, Cianci M, Foos N, Silva CS, Mazzei L, Zubieta C, de Maria A, Nanao MH

Acta crystallographica. Section D, Structural biology 2016

27599735. doi:10.1107/s2059798316012079.

Noncovalent PEGylation via Lectin-Glycopolymer Interactions

Antonik PM, Eissa AM, Round AR, Cameron NR, Crowley PB

Biomacromolecules 2016

27403588. doi:10.1021/acs.biomac.6b00766.

RoboDiff: combining a sample changer and goniometer for highly automated macromolecular crystallography experiments.

Nurizzo D, Bowler MW, Caserotto H, Dobias F, Giraud T, Surr J, Guichard N, Papp G, Guijarro M, Mueller-Dieckmann C, Flot D, McSweeney S, Cipriani F, Theveneau P, Leonard GA

Acta crystallographica. Section D, Structural biology 2016

27487827. doi:10.1107/s205979831601158x.

Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites

Simon B, Huart A, Temmerman K, Vahokoski J, Mertens H, Komadina D, Hoffmann J, Yumerefendi H, Svergun D, Kursula P, Schultz C, McCarthy A, Hart D, Wilmanns M

Structure 2016

27133022. doi:10.1016/j.str.2016.03.020.

Mechanism of Sulfur Transfer Across Protein-Protein Interfaces: The Cysteine Desulfurase Model System

Fernandez FJ, Arda A, Lopez-Estepa M, Aranda J, Pena-Soler E, Garces F, Round A, Campos-Olivas R, Bruix M, Coll M, Tunon I, Jimenez-Barbero J, Vega MC

ACS CATALYSIS 2016

doi:10.1021/acscatal.6b00360.

Structural basis of suppression of host translation termination by Moloney Murine Leukemia Virus.

Tang X, Zhu Y, Baker SL, Bowler MW, Chen BJ, Chen C, Hogg JR, Goff SP, Song H

Nature communications 2016

27329342. doi:10.1038/ncomms12070.

The Use of Small-Angle Scattering for the Characterization of Multi Subunit Complexes.

Round A

Advances in experimental medicine and biology 2016

27165335. doi:10.1007/978-3-319-27216-0_21.

The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics.

Sari D, Gupta K, Raj DB, Aubert A, Drncová P, Garzoni F, Fitzgerald D, Berger I

Advances in experimental medicine and biology 2016

27165327. doi:10.1007/978-3-319-27216-0_13.

The Myb domain of LUX ARRHYTHMO in complex with DNA: expression, purification and crystallization

Silva CS, Lai XL, Nanao M, Zubieta C

Acta crystallographica. Section F, Structural biology communications 2016

27139826. doi:10.1107/s2053230x16004684.

The Nucleosome Remodeling and Deacetylase Complex NuRD is built from preformed catalytically active sub-modules.

Zhang W, Aubert A, Gomez de Segura JM, Karuppasamy M, Basu S, Murthy A, Diamante A, Drury TA, Balmer J, Cramard J, Watson AA, Lando D, Lee SF, Palayret M, Kloet S, Smits A, Deery M, Vermeulen M, Hendrich B, Klenerman D, Schaffitzel C, Berger I, Laue ED

Journal of molecular biology 2016

27117189. doi:10.1016/j.jmb.2016.04.025.

A SAM oligomerization domain shapes the genomic binding landscape of the LEAFY transcription factor.

Sayou C, Nanao MH, Jamin M, Posé D, Thévenon E, Grégoire L, Tichtinsky G, Denay G, Ott F, Peirats Llobet M, Schmid M, Dumas R, Parcy F

Nature communications 2016

27097556. doi:10.1038/ncomms11222.

Zooming In On Transcription Preinitiation.

Gupta K, Sari-Ak D, Haffke M, Trowitzsch S, Berger I

Journal of molecular biology 2016

27067110. doi:10.1016/j.jmb.2016.04.003.

Influenza Polymerase Can Adopt an Alternative Configuration Involving a Radical Repacking of PB2 Domains.

Thierry E, Guilligay D, Kosinski J, Bock T, Gaudon S, Round A, Pflug A, Hengrung N, El Omari K, Baudin F, Hart DJ, Beck M, Cusack S

Molecular cell 2015

26711008. doi:10.1016/j.molcel.2015.11.016.

Fully automatic macromolecular crystallography: the impact of MASSIF-1 on the optimum acquisition and quality of data

Bowler MW, Svensson O, Nurizzo D

2016

doi:10.1080/0889311X.2016.1155050.

Radiation-damage-induced phasing: a case study using UV irradiation with light-emitting diodes.

de Sanctis D, Zubieta C, Felisaz F, Caserotto H, Nanao MH

Acta crystallographica. Section D, Structural biology 2016

26960126. doi:10.1107/s2059798315021658.

Domain organization of vaccinia virus helicase-primase D5.

Hutin S, Ling WL, Round A, Effantin G, Reich S, Iseni F, Tarbouriech N, Schoehn G, Burmeister WP

Journal of virology 2016

26912611. doi:10.1128/JVI.00044-16.

Online data analysis at the ESRF bioSAXS beamline, BM29

Brennich ME, Kieffer J, Bonamis G, Antolinos AD, Hutin S, Pernot P, Round A

JOURNAL OF APPLIED CRYSTALLOGRAPHY 2016

doi:10.1107/S1600576715024462.

The solution configurations of inactive and activated DntR have implications for the sliding dimer mechanism of LysR transcription factors.

Lerche M, Dian C, Round A, Lönneborg R, Brzezinski P, Leonard GA

Scientific reports 2016

26817994. doi:10.1038/srep19988.

FANCM interacts with PCNA to promote replication traverse of DNA interstrand crosslinks.

Rohleder F, Huang J, Xue Y, Kuper J, Round A, Seidman M, Wang W, Kisker C

Nucleic acids research 2016

26825464. doi:10.1093/nar/gkw037.

PIWI Slicing and EXD1 Drive Biogenesis of Nuclear piRNAs from Cytosolic Targets of the Mouse piRNA Pathway.

Yang Z, Chen KM, Pandey RR, Homolka D, Reuter M, Janeiro BK, Sachidanandam R, Fauvarque MO, McCarthy AA, Pillai RS

Molecular cell 2015

26669262. doi:10.1016/j.molcel.2015.11.009.

Evolution of the Plant Reproduction Master Regulators LFY and the MADS Transcription Factors: The Role of Protein Structure in the Evolutionary Development of the Flower.

Silva CS, Puranik S, Round A, Brennich M, Jourdain A, Parcy F, Hugouvieux V, Zubieta C

Frontiers in plant science 2015

26779227. doi:10.3389/fpls.2015.01193.

In situ hydrothermal syntheses, structures and photoluminescent properties of four novel metal-organic frameworks constructed by lanthanide (Ln=Ce(III), Pr(III), Eu(III)) and Cu(I) metals with flexible dicarboxylate acids and piperazine-based ligands

Ay B, Karaca S, Yildiz E, Lopez V, Nanao MH, Zubieta J

JOURNAL OF SOLID STATE CHEMISTRY 2016

doi:10.1016/j.jssc.2015.10.033.

Structural and Functional Highlights of Vacuolar Soluble Protein 1 from Pathogen Trypanosoma brucei brucei.

Jamwal A, Round AR, Bannwarth L, Venien-Bryan C, Belrhali H, Yogavel M, Sharma A

The Journal of biological chemistry 2015

26494625. doi:10.1074/jbc.m115.674176.

Asymmetric ring structure of Vps4 required for ESCRT-III disassembly.

Caillat C, Macheboeuf P, Wu Y, McCarthy AA, Boeri-Erba E, Effantin G, Göttlinger HG, Weissenhorn W, Renesto P

Nature communications 2015

26632262. doi:10.1038/ncomms9781.

MASSIF-1: a beamline dedicated to the fully automatic characterization and data collection from crystals of biological macromolecules.

Bowler MW, Nurizzo D, Barrett R, Beteva A, Bodin M, Caserotto H, Delagenière S, Dobias F, Flot D, Giraud T, Guichard N, Guijarro M, Lentini M, Leonard GA, McSweeney S, Oskarsson M, Schmidt W, Snigirev A, von Stetten D, Surr J, Svensson O, Theveneau P, Mueller-Dieckmann C

Journal of synchrotron radiation 2015

26524320. doi:10.1107/S1600577515016604.

Crystallization and X-ray diffraction studies of a complete bacterial fatty-acid synthase type I.

Enderle M, McCarthy A, Paithankar KS, Grininger M

Acta crystallographica. Section F, Structural biology communications 2015

26527268. doi:10.1107/s2053230x15018336.

Towards phasing using high X-ray intensity.

Galli L, Son SK, Barends TR, White TA, Barty A, Botha S, Boutet S, Caleman C, Doak RB, Nanao MH, Nass K, Shoeman RL, Timneanu N, Santra R, Schlichting I, Chapman HN

IUCrJ 2015

26594370. doi:10.1107/s2052252515014049.

Hydrothermal synthesis and crystal and molecular structure of a tetranuclear cobalt(II) tetracarboxylporphyrin cluster

Hagrman D, Garner D, Nanao MH, Zubieta J

INORGANIC CHEMISTRY COMMUNICATIONS 2015

doi:10.1016/j.inoche.2015.10.008.

MeshAndCollect: an automated multi-crystal data-collection workflow for synchrotron macromolecular crystallography beamlines.

Zander U, Bourenkov G, Popov AN, de Sanctis D, Svensson O, McCarthy AA, Round E, Gordeliy V, Mueller-Dieckmann C, Leonard GA

Acta crystallographica. Section D, Biological crystallography 2015

26527148. doi:10.1107/s1399004715017927.

Large Scale Conformational Dynamics Control H5N1 Influenza Polymerase PB2 Binding to Importin α.

Delaforge E, Milles S, Bouvignies G, Bouvier D, Boivin S, Salvi N, Maurin D, Martel A, Round A, Lemke EA, Jensen MR, Hart D, Blackledge M

Journal of the American Chemical Society 2015

26424125. doi:10.1021/jacs.5b07765.

Low resolution structural studies of apoferritin via SANS and SAXS: the effect of concentration

Murugova TN, Vlasov AV, Ivankov OI, Rogachev AV, Ryzhykau YL, Soloviov DV, Zhigunov A, Zinovev EV, Kovalev YS, Round A, Gordeliy VI, Kuklin AI

JOURNAL OF OPTOELECTRONICS AND ADVANCED MATERIALS 2015

doi:.

Fully automatic characterization and data collection from crystals of biological macromolecules.

Svensson O, Malbet-Monaco S, Popov A, Nurizzo D, Bowler MW

Acta crystallographica. Section D, Biological crystallography 2015

26249356. doi:10.1107/s1399004715011918.

Purification and characterization of DR_2577 (SlpA) a major S-layer protein from Deinococcus radiodurans.

Farci D, Bowler MW, Esposito F, McSweeney S, Tramontano E, Piano D

Frontiers in microbiology 2015

26074883. doi:10.3389/fmicb.2015.00414.

The sweet quartet: Binding of fucose to the norovirus capsid.

Koromyslova AD, Leuthold MM, Bowler MW, Hansman GS

Virology 2015

25980740. doi:10.1016/j.virol.2015.04.006.

Data Analysis WorkbeNch (DAWN)

Basham M, Filik J, Wharmby MT, Chang PCY, El Kassaby B, Gerring M, Aishima J, Levik K, Pulford BCA, Sikharulidze I, Sneddon D, Webber M, Dhesi SS, Maccherozzi F, Svensson O, Brockhauser S, Naray G, Ashton AW

JOURNAL OF SYNCHROTRON RADIATION 2015

25931106. doi:10.1107/S1600577515002283.

Electronic damage in S atoms in a native protein crystal induced by an intense X-ray free-electron laser pulse

Galli L, Son SK, Klinge M, Bajt S, Barty A, Bean R, Betzel C, Beyerlein KR, Caleman C, Doak RB, Duszenko M, Fleckenstein H, Gati C, Hunt B, Kirian RA, Liang M, Nanao MH, Nass K, Oberthur D, Redecke L, Shoeman R, Stellato F, Yoon CH, White TA, Yefanov O, Spence J, Chapman HN

Structural Dynamics 2015

26798803. doi:10.1063/1.4919398.

The Crystal Structure and Small-Angle X-Ray Analysis of CsdL/TcdA Reveal a New tRNA Binding Motif in the MoeB/E1 Superfamily.

López-Estepa M, Ardá A, Savko M, Round A, Shepard WE, Bruix M, Coll M, Fernández FJ, Jiménez-Barbero J, Vega MC

PloS one 2015

25897750. doi:10.1371/journal.pone.0118606.

The pentameric nucleoplasmin fold is present in Drosophila FKBP39 and a large number of chromatin-related proteins.

Edlich-Muth C, Artero JB, Callow P, Przewloka MR, Watson AA, Zhang W, Glover DM, Debski J, Dadlez M, Round AR, Trevor Forsyth V, Laue ED

Journal of molecular biology 2015

25813344. doi:10.1016/j.jmb.2015.03.010.

Metazoan Maelstrom is an RNA-binding protein that has evolved from an ancient nuclease active in protists.

Chen KM, Campbell E, Pandey RR, Yang Z, McCarthy AA, Pillai RS

2015

25778731. doi:10.1261/rna.049437.114.

Structure and dynamics of the MKK7-JNK signaling complex.

Kragelj J, Palencia A, Nanao MH, Maurin D, Bouvignies G, Blackledge M, Jensen MR

2015

25737554. doi:10.1073/pnas.1419528112.

Automation and Experience of Controlled Crystal Dehydration: Results from the European Synchrotron HC1 Collaboration

Bowler MW, Mueller U, Weiss MS, Sanchez-Weatherby J, Sorensen TLM, Thunnissen MMGM, Ursby T, Gobbo A, Russi S, Bowler MG, Brockhauser S, Svensson O, Cipriani F

CRYSTAL GROWTH & DESIGN 2015

doi:10.1021/cg500890r.

Towards RIP using free-electron laser SFX data.

Galli L, Son SK, White TA, Santra R, Chapman HN, Nanao MH

Journal of synchrotron radiation 2015

25723926. doi:10.1107/s1600577514027854.

Evolution. Response to Comment on "A promiscuous intermediate underlies the evolution of LEAFY DNA binding specificity".

Brockington SF, Moyroud E, Sayou C, Monniaux M, Nanao MH, Thévenon E, Chahtane H, Warthmann N, Melkonian M, Zhang Y, Wong GK, Weigel D, Dumas R, Parcy F

Science (New York, N.Y.) 2015

25657241. doi:10.1126/science.1256011.

ISPyB for BioSAXS, the gateway to user autonomy in solution scattering experiments.

De Maria Antolinos A, Pernot P, Brennich ME, Kieffer J, Bowler MW, Delageniere S, Ohlsson S, Malbet Monaco S, Ashton A, Franke D, Svergun D, McSweeney S, Gordon E, Round A

Acta crystallographica. Section D, Biological crystallography 2015

25615862. doi:10.1107/s1399004714019609.

The status of the macromolecular crystallography beamlines at the European Synchrotron Radiation Facility

Mueller-Dieckmann C, Bowler MW, Carpentier P, Flot D, McCarthy AA, Nanao MH, Nurizzo D, Pernot P, Popov A, Round A, Royant A, de Sanctis D, von Stetten D, Leonard GA

EUROPEAN PHYSICAL JOURNAL PLUS 2015

doi:10.1140/epjp/i2015-15070-0.

BioSAXS Sample Changer: a robotic sample changer for rapid and reliable high-throughput X-ray solution scattering experiments.

Round A, Felisaz F, Fodinger L, Gobbo A, Huet J, Villard C, Blanchet CE, Pernot P, McSweeney S, Roessle M, Svergun DI, Cipriani F

2015

25615861. doi:10.1107/S1399004714026959.

Structural insight into cap-snatching and RNA synthesis by influenza polymerase.

Reich S, Guilligay D, Pflug A, Malet H, Berger I, Crépin T, Hart D, Lunardi T, Nanao M, Ruigrok RW, Cusack S

Nature 2014

25409151. doi:10.1038/nature14009.

Mammalian translation elongation factor eEF1A2: X-ray structure and new features of GDP/GTP exchange mechanism in higher eukaryotes.

Crepin T, Shalak VF, Yaremchuk AD, Vlasenko DO, McCarthy A, Negrutskii BS, Tukalo MA, El'skaya AV

Nucleic acids research 2014

25326326. doi:10.1093/nar/gku974.

A new calmodulin-binding motif for inositol 1,4,5-trisphosphate 3-kinase regulation.

Franco-Echevarría E, Baños-Sanz JI, Monterroso B, Round A, Sanz-Aparicio J, González B

The Biochemical journal 2014

25101901. doi:10.1042/bj20140757.

Protein assembly mediated by sulfonatocalix[4]arene.

McGovern RE, McCarthy AA, Crowley PB

Chemical communications (Cambridge, England) 2014

25068633. doi:10.1039/c4cc04897k.

Structural Basis for the Oligomerization of the MADS Domain Transcription Factor SEPALLATA3 in Arabidopsis

Puranik S, Acajjaoui S, Conn S, Costa L, Conn V, Vial A, Marcellin R, Melzer R, Brown E, Hart D, Theißen G, Silva CS, Parcy F, Dumas R, Nanao, M, Zubieta C

The Plant cell 2014

25228343. doi:10.1105/tpc.114.127910.

α-Fluorophosphonates reveal how a phosphomutase conserves transition state conformation over hexose recognition in its two-step reaction.

Jin Y, Bhattasali D, Pellegrini E, Forget SM, Baxter NJ, Cliff MJ, Bowler MW, Jakeman DL, Blackburn GM, Waltho JP

Proceedings of the National Academy of Sciences of the United States of America 2014

25104750. doi:10.1073/pnas.1402850111.

Structure of the C. elegans ZYG-1 Cryptic Polo Box Suggests a Conserved Mechanism for Centriolar Docking of Plk4 Kinases

Shimanovskaya E, Viscardi V, Lesigang J, Lettman MM, Qiao R, Svergun DI, Round A, Oegema K, Dong G

Structure (London, England : 1993) 2014

24980795. doi:10.1016/j.str.2014.05.009.

New features of the cell wall of the radio-resistant bacterium Deinococcus radiodurans

Farci D, Bowler MW, Kirkpatrick J, McSweeney S, Tramontano E, Piano D

Biochimica et biophysica acta 2014

24589688. doi:10.1016/j.bbamem.2014.02.014.

The crystal structure of the Split End protein SHARP adds a new layer of complexity to proteins containing RNA recognition motifs

Arieti F, Gabus C, Tambalo M, Huet T, Round A, Thore S

Nucleic acids research 2014

24748666. doi:10.1093/nar/gku277.

The MID-PIWI module of Piwi proteins specifies nucleotide- and strand-biases of piRNAs

Cora E, Pandey RR, Xiol J, Taylor J, Sachidanandam R, McCarthy AA, Pillai RS

RNA (New York, N.Y.) 2014

24757166. doi:10.1261/rna.044701.114.

Molecular basis for AUXIN RESPONSE FACTOR protein interaction and the control of auxin response repression.

Korasick DA, Westfall CS, Lee SG, Nanao MH, Dumas R, Hagen G, Guilfoyle TJ, Jez JM, Strader LC

Proceedings of the National Academy of Sciences of the United States of America 2014

24706860. doi:10.1073/pnas.1400074111.

Structural basis for oligomerization of auxin transcriptional regulators

Nanao MH, Vinos-Poyo T, Brunoud G, Thévenon E, Mazzoleni M, Mast D, Lainé S, Wang S, Hagen G, Li H, Guilfoyle TJ, Parcy F, Vernoux T, Dumas R

Nature communications 2014

24710426. doi:10.1038/ncomms4617.

A promiscuous intermediate underlies the evolution of LEAFY DNA binding specificity

Sayou C, Monniaux M, Nanao MH, Moyroud E, Brockington SF, Thévenon E, Chahtane H, Warthmann N, Melkonian M, Zhang Y, Wong GK, Weigel D, Parcy F, Dumas R

Science (New York, N.Y.) 2014

24436181. doi:10.1126/science.1248229.

Lsm2 and Lsm3 bridge the interaction of the Lsm1-7 complex with Pat1 for decapping activation

Wu D, Muhlrad D, Bowler MW, Jiang S, Liu Z, Parker R, Song H

Cell research 2013

24247251. doi:10.1038/cr.2013.152.

Measurement of the intrinsic variability within protein crystals: implications for sample-evaluation and data-collection strategies

Bowler MG, Bowler MW

Acta crystallographica. Section F, Structural biology communications 2014

24419635. doi:10.1107/s2053230x13032007.

QsIA disrupts LasR dimerization in antiactivation of bacterial quorum sensing

Fan H, Dong Y, Wu D, Bowler MW, Zhang L, Song H

Proceedings of the National Academy of Sciences of the United States of America 2013

24319092. doi:10.1073/pnas.1314415110.

Predicting the X-ray lifetime of protein crystals

Zeldin OB, Brockhauser S, Bremridge J, Holton JM, Garman EF

Proceedings of the National Academy of Sciences of the United States of America 2013

24297937. doi:10.1073/pnas.1315879110.

Determination of the GH3.12 protein conformation through HPLC-integrated SAXS measurements combined with X-ray crystallography

Round A, Brown E, Marcellin R, Kapp U, Westfall CS, Jez JM, Zubieta C

Acta crystallographica. Section D, Biological crystallography 2013

24100325. doi:10.1107/s0907444913019276.

An 'open' structure of the RecOR complex supports ssDNA binding within the core of the complex

Radzimanowski J, Dehez F, Round A, Bidon-Chanal A, McSweeney S, Timmins J

Nucleic acids research 2013

23814185. doi:10.1093/nar/gkt572.

The use of a mini-κ goniometer head in macromolecular crystallography diffraction experiments

Brockhauser S, Ravelli RB, McCarthy AA

Acta crystallographica. Section D, Biological crystallography 2013

23793150. doi:10.1107/s0907444913003880.

Recent progress in robot-based systems for crystallography and their contribution to drug discovery

Ferrer JL, Larive NA, Bowler MW, Nurizzo D

Expert opinion on drug discovery 2013

23656378. doi:10.1517/17460441.2013.793666.

Upgraded ESRF BM29 beamline for SAXS on macromolecules in solution

Pernot P, Round A, Barrett R, De Maria Antolinos A, Gobbo A, Gordon E, Huet J, Kieffer J, Lentini M, Mattenet M, Morawe C, Mueller-Dieckmann C, Ohlsson S, Schmid W, Surr J, Theveneau P, Zerrad L, McSweeney S

Journal of synchrotron radiation 2013

23765312. doi:10.1107/s0909049513010431.

Conformational dynamics in phosphoglycerate kinase, an open and shut case?

Bowler MW

FEBS letters 2013

23684636. doi:10.1016/j.febslet.2013.05.012.

Automatic processing of macromolecular crystallography X-ray diffraction data at the ESRF

Monaco S, Gordon E, Bowler MW, Delagenière S, Guijarro M, Spruce D, Svensson O, McSweeney SM, McCarthy AA, Leonard G, Nanao MH

Journal of applied crystallography 2013

23682196. doi:10.1107/s0021889813006195.

Crystal structure and mechanism of activation of TANK-binding kinase 1

Larabi A, Devos JM, Ng SL, Nanao MH, Round A, Maniatis T, Panne D

Cell reports 2013

23453971. doi:10.1016/j.celrep.2013.01.034.

Le rayonnement synchrotron : comprendre la relation structure-fonction des macromolecules biologiques

Bowler MW, Nurizzo D

Reflets de la physique 2013

doi:10.1051/refdp/201334048.

Quaternary structure of the yeast Arc1p-aminoacyl-tRNA synthetase complex in solution and its compaction upon binding of tRNAs

Koehler C, Round A, Simader H, Suck D, Svergun D

Nucleic acids research 2013

23161686. doi:10.1093/nar/gks1072.

Charge-balanced metal fluoride complexes for protein kinase A with adenosine diphosphate and substrate peptide SP20

Jin Y, Cliff MJ, Baxter NJ, Dannatt HR, Hounslow AM, Bowler MW, Blackburn GM, Waltho JP

Angewandte Chemie (International ed. in English) 2012

23125010. doi:10.1002/anie.201204266.

Structural basis of the PNRC2-mediated link between mrna surveillance and decapping

Lai T, Cho H, Liu Z, Bowler MW, Piao S, Parker R, Kim YK, Song H

Structure (London, England : 1993) 2012

23085078. doi:10.1016/j.str.2012.09.009.

The multiple Tudor domain-containing protein TDRD1 is a molecular scaffold for mouse Piwi proteins and piRNA biogenesis factors

Mathioudakis N, Palencia A, Kadlec J, Round A, Tripsianes K, Sattler M, Pillai RS, Cusack S

RNA (New York, N.Y.) 2012

22996915. doi:10.1261/rna.034181.112.

Reflections on biocatalysis involving phosphorus

Blackburn GM, Bowler MW, Jin Y, Waltho JP

Biochemistry. Biokhimiia 2012

23157289. doi:10.1134/s000629791210001x.

Segmenting data sets for RIP

de Sanctis D, Nanao MH

Acta crystallographica. Section D, Biological crystallography 2012

22948916. doi:10.1107/s0907444912023475.

A structural basis for the biosynthesis of the major chlorogenic acids found in coffee

Lallemand LA, Zubieta C, Lee SG, Wang Y, Acajjaoui S, Timmins J, McSweeney S, Jez JM, McCarthy JG, McCarthy AA

Plant physiology 2012

22822210. doi:10.1104/pp.112.202051.

The use of workflows in the design and implementation of complex experiments in macromolecular crystallography

Brockhauser S, Svensson O, Bowler MW, Nanao M, Gordon E, Leal RM, Popov A, Gerring M, McCarthy AA, Gotz A

Acta crystallographica. Section D, Biological crystallography 2012

22868763. doi:10.1107/s090744491201863x.

Purification, crystallization and preliminary X-ray diffraction analysis of a hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyltransferase (HCT) from Coffea canephora involved in chlorogenic acid biosynthesis

Lallemand LA, McCarthy JG, McSweeney S, McCarthy AA

Acta crystallographica. Section F, Structural biology and crystallization communications 2012

22750875. doi:10.1107/s1744309112019082.

Structural basis for prereceptor modulation of plant hormones by GH3 proteins

Westfall CS, Zubieta C, Herrmann J, Kapp U, Nanao MH, Jez JM

Science (New York, N.Y.) 2012

22628555. doi:10.1126/science.1221863.

A high-affinity, dimeric inhibitor of PSD-95 bivalently interacts with PDZ1-2 and protects against ischemic brain damage

Bach A, Clausen BH, Møller M, Vestergaard B, Chi CN, Round A, Sørensen PL, Nissen KB, Kastrup JS, Gajhede M, Jemth P, Kristensen AS, Lundström P, Lambertsen KL, Strømgaard K

Proceedings of the National Academy of Sciences of the United States of America 2012

22343531. doi:10.1073/pnas.1113761109.

Chromodomains read the arginine code of post-translational targeting

Holdermann I, Meyer NH, Round A, Wild K, Sattler M, Sinning I

Nature structural & molecular biology 2012

22231402. doi:10.1038/nsmb.2196.

Structural and functional insights into endoglin ligand recognition and binding

Alt A, Miguel-Romero L, Donderis J, Aristorena M, Blanco FJ, Round A, Rubio V, Bernabeu C, Marina A

PloS one 2012

22347366. doi:10.1371/journal.pone.0029948.

Macro-to-micro structural proteomics: native source proteins for high-throughput crystallization

Totir M, Echols N, Nanao M, Gee CL, Moskaleva A, Gradia S, Iavarone AT, Berger JM, May AP, Zubieta C, Alber T

PloS one 2012

22393408. doi:10.1371/journal.pone.0032498.

Measurement of the equilibrium relative humidity for common precipitant concentrations: facilitating controlled dehydration experiments

Wheeler MJ, Russi S, Bowler MG, Bowler MW

Acta crystallographica. Section F, Structural biology and crystallization communications 2012

22232186. doi:10.1107/s1744309111054029.

ISPyB: an information management system for synchrotron macromolecular crystallography

Delagenière S, Brenchereau P, Launer L, Ashton AW, Leal R, Veyrier S, Gabadinho J, Gordon EJ, Jones SD, Levik KE, McSweeney SM, Monaco S, Nanao M, Spruce D, Svensson O, Walsh MA, Leonard GA

Bioinformatics (Oxford, England) 2011

21949273. doi:10.1093/bioinformatics/btr535.

Structural basis for the activation of innate immune pattern-recognition receptor RIG-I by viral RNA

Kowalinski E, Lunardi T, McCarthy AA, Louber J, Brunel J, Grigorov B, Gerlier D, Cusack S

Cell 2011

22000019. doi:10.1016/j.cell.2011.09.039.

Gold nanoparticle labeling of cells is a sensitive method to investigate cell distribution and migration in animal models of human disease

Menk RH, Schültke E, Hall C, Arfelli F, Astolfo A, Rigon L, Round A, Ataelmannan K, MacDonald SR, Juurlink BH

Nanomedicine : nanotechnology, biology, and medicine 2011

21333753. doi:10.1016/j.nano.2011.01.010.

Burkholderia cenocepacia BC2L-C is a super lectin with dual specificity and proinflammatory activity

Sulák O, Cioci G, Lameignère E, Balloy V, Round A, Gutsche I, Malinovská L, Chignard M, Kosma P, Aubert DF, Marolda CL, Valvano MA, Wimmerová M, Imberty A

PLoS pathogens 2011

21909279. doi:10.1371/journal.ppat.1002238.

Unique structure of iC3b resolved at a resolution of 24 A by 3D-electron microscopy

Alcorlo M, Martínez-Barricarte R, Fernández FJ, Rodríguez-Gallego C, Round A, Vega MC, Harris CL, de Cordoba SR, Llorca O

Proceedings of the National Academy of Sciences of the United States of America 2011

21788512. doi:10.1073/pnas.1106746108.

Translation calibration of inverse-kappa goniometers in macromolecular crystallography

Brockhauser S, White KI, McCarthy AA, Ravelli RB

Acta crystallographica. Section A, Foundations of crystallography 2011

21487180. doi:10.1107/s0108767311004831.

A spring-loaded release mechanism regulates domain movement and catalysis in phosphoglycerate kinase

Zerrad L, Merli A, Schröder GF, Varga A, Gráczer É, Pernot P, Round A, Vas M, Bowler MW

The Journal of biological chemistry 2011

21349853. doi:10.1074/jbc.m110.206813.

Inducing phase transitions in protein crystals by controlled crystal dehydration.

Silvia R, Babu M, McCarthy A, Cipriani F, Hassan B

2011

doi:10.1107/S010876731107944X.

Visualizing the metal-binding versatility of copper trafficking sites

Badarau A, Firbank SJ, McCarthy AA, Banfield MJ, Dennison C

Biochemistry 2010

20726513. doi:10.1021/bi101064w.

MxCuBE: a synchrotron beamline control environment customized for macromolecular crystallography experiments

Gabadinho J, Beteva A, Guijarro M, Rey-Bakaikoa V, Spruce D, Bowler MW, Brockhauser S, Flot D, Gordon EJ, Hall DR, Lavault B, McCarthy AA, McCarthy J, Mitchell E, Monaco S, Mueller-Dieckmann C, Nurizzo D, Ravelli RB, Thibault X, Walsh MA, Leonard GA, McSweeney SM

Journal of synchrotron radiation 2010

20724792. doi:10.1107/s0909049510020005.

Structural and mechanistic insights into Helicobacter pylori NikR activation

Bahlawane C, Dian C, Muller C, Round A, Fauquant C, Schauer K, de Reuse H, Terradot L, Michaud-Soret I

Nucleic acids research 2010

20089510. doi:10.1093/nar/gkp1216.

Structural basis of HIV-1 tethering to membranes by the BST-2/tetherin ectodomain

Hinz A, Miguet N, Natrajan G, Usami Y, Yamanaka H, Renesto P, Hartlieb B, McCarthy AA, Simorre JP, Göttlinger H, Weissenhorn W

Cell host & microbe 2010

20399176. doi:10.1016/j.chom.2010.03.005.

Full-length extracellular region of the var2CSA variant of PfEMP1 is required for specific, high-affinity binding to CSA

Srivastava A, Gangnard S, Round A, Dechavanne S, Juillerat A, Raynal B, Faure G, Baron B, Ramboarina S, Singh SK, Belrhali H, England P, Lewit-Bentley A, Scherf A, Bentley GA, Gamain B

Proceedings of the National Academy of Sciences of the United States of America 2010

20194779. doi:10.1073/pnas.1000951107.

New beamline dedicated to solution scattering from biological macromolecules at the ESRF

Pernot P, Theveneau P, Giraud T, Fernandes NR, Nurizzo D, Spruce D, Surr J, McSweeney S, Round A, Felisaz F, Foedinger L, Gobbo A, Huet J, Villard C, Cipriani F

Journal of physics. Conference series 2010

doi:10.1088/1742-6596/247/1/012009.

The abscisic acid receptor PYR1 in complex with abscisic acid

Santiago J, Dupeux F, Round A, Antoni R, Park SY, Jamin M, Cutler SR, Rodriguez PL, Márquez JA

Nature 2009

19898494. doi:10.1038/nature08591.

A decade of user operation on the macromolecular crystallography MAD beamline ID14-4 at the ESRF

McCarthy AA, Brockhauser S, Nurizzo D, Theveneau P, Mairs T, Spruce D, Guijarro M, Lesourd M, Ravelli RB, McSweeney S

Journal of synchrotron radiation 2009

19844017. doi:10.1107/s0909049509035377.

Structure and functional relevance of the Slit2 homodimerization domain

Seiradake E, von Philipsborn AC, Henry M, Fritz M, Lortat-Jacob H, Jamin M, Hemrika W, Bastmeyer M, Cusack S, McCarthy AA

EMBO reports 2009

19498462. doi:10.1038/embor.2009.95.

Structure of SRP14 from the Schizosaccharomyces pombe signal recognition particle

Brooks MA, Ravelli RB, McCarthy AA, Strub K, Cusack S

Acta crystallographica. Section D, Biological crystallography 2009

19390147. doi:10.1107/s0907444909005484.

X-ray tomographic reconstruction of macromolecular samples

Brockhauser S, Di Michiel M, McGeehan JE, McCarthy AA, Ravelli RBG

Journal of applied crystallography 2008

doi:10.1107/S002188980802935X.

Capturing the Shape of Macromolecular Crystals in 3D

Leal RMF, Teixeira SCM, Rey V, Forsyth VT, Mitchel EP, Brockhauser S, Di Michiel M, McGeehan JE, McCarthy A, Ravelli RBG

2008

doi:.