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EMBL Imaging Centre

Cutting-edge electron and light microscopy technologies

MINFLUX

Important note: This system will be available in open access after the opening of the EMBL IC.

With the first commercially available MINFLUX from Abberior Instruments, the EMBL IC enables scientists to image a broad range of biological samples (living/fixed) with a lateral resolution of < 5 nm. This allows to study among others the structure of protein complexes like nuclear pores with unprecedented resolution. Additionally, MINFLUX tracking allows to study movement of single molecules in living cells with up to 10 kHz sampling rate (one localisation each 100 µs, which is  up to 100 x faster than conventional camera-based tracking). 


Unique features of our MINFLUX:

  • < 5 nm localisation precision (in x, y dependent on fluorophore, imaging mode)
  • tracking of single molecules with up to 10 kHz (one datapoint every 100 µs) featuring 20 nm resolution 
  • Nanometre-level active sample stabilization system based on fiducial markers in the sample

Important specifications of our MINFLUX:

  • Laser lines: MINFLUX: 640 nm, 561 nm, Confocal: 488 nm, 405 nm
  • Two-colour ratiometric MINFLUX imaging with 640 nm or 560 nm excitation
  • IX83 inverted microscope stand equipped with a 1.4 NA 100x oil objective and 1.35 NA 100x silicon oil objective lens
Fluorescently labeled nuclear pore proteins imaged by confocal microscopy, stimulated emission depletion (STED) microscopy and fluorophore coordinate detection by minimal emission fluxes (MINFLUX). For the MINFLUX images a different sample and label is shown.  (Credit:Sebastian Schnorrenberg/EMBL Imaging Centre)
Tracking of Atto647N coupled lipids in artificial lipid bilayers; Tracking speed: 10 kHz (Datapoint every 100µs)
(Credit:Sebastian Schnorrenberg/EMBL Imaging Centre)

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