Crossbeam 550
The Crossbeam 550 (focused ion beam-scanning electron microscope) from ZEISS allows data acquisition in 3 dimensions at isotropic nanometer voxel size. Samples from cells to tissues and organisms can be looked at with electron microscopic detail. Chemically fixed, high-pressure frozen and freeze-substituted as well as purely frozen samples can be analyzed. On top of data production, the Crossbeam550 is also equipped to generate cryo-lamellae to be analyzed further in a transmission electron microscope e.g. using tomography.
Unique features
- Isotropic volume acquisition down to a few nm
- Large 3D volume acquisition
- Cryo lamella preparation
Important specifications
- Detectors: InLens, EsB and Se
- Quorum Cryo-SEM preparation system
- Zeiss Atlas 5 software
Aquilos 2
The Aquilos 2 (focused ion beam – scanning electron microscope) from Thermo Fisher Scientific enables the preparation of cellular lamellae from vitreous samples suitable for high-resolution cryo-electron tomography analyses. Plunge frozen as well as high-pressure frozen samples can be processed at cryo temperature by standard FIB milling and cryo-lift out approaches, respectively. Automated FIB milling routines based on commercial and in-house developed software packages enable unsupervised overnight operations for high-throughput milling.
Unique features
- Automated cryo lamella preparation
- Nanomanipulator for cryo-lift out
- Integrated widefield fluorescence microscopy
Important specifications
- Serial-FIB and AutoTEM software for automated lamella preparation
- EasyLift NanoManipulator
- Integrated Fluorescence Light Microscope (iFLM)
