Crossbeam 550
The Crossbeam 550 (focused ion beam-scanning electron microscope) from ZEISS allows data acquisition in 3 dimensions at isotropic nanometer voxel size. Samples from cells to tissues and organisms can be looked at with electron microscopic detail. Chemically fixed, high-pressure frozen and freeze-substituted as well as purely frozen samples can be analysed. On top of data production, the Crossbeam550 is also equipped to generate cryo-lamellae to be analysed further in a transmission electron microscope e.g. using tomography.
Unique features
- Isotropic volume acquisition down to a few nm
- Large 3D volume acquisition
- Cryo lamella preparation
Important specifications
- Detectors: InLens, EsB and Se
- Quorum Cryo-SEM preparation system
- ZEISS Atlas 5 software
Titan Krios G4
The Titan Krios G4 from Thermo Fisher Scientific is a 300 kV transmission electron microscope (TEM) with a three-condenser lens system. It features the latest technology for high-end single-particle and tomography data acquisition including a cold field emission gun (C-FEG), SelectrisX energy filter, Falcon 4 electron counting camera and volta phase plate. It’s configured for high-throughput automated single particle and tomography data acquisition.
Unique features
- C-FEG electron source with narrow energy spread
- State-of-the-art energy filter and electron counting camera
- phase plate
- Autoloader system for easy sample transfer between TFS TEMs
Important specifications
- 300 kV C-FEG electron source
- SelectrisX energy filter
- Falcon 4EC direct electron detector
- Volta phase plate
- SerialEM software for tailored, automated data acquisition
Aquilos 2
The Aquilos 2 (focused ion beam – scanning electron microscope) from Thermo Fisher Scientific enables the preparation of cellular lamellae from vitreous samples suitable for high-resolution cryo-electron tomography analyses. Plunge frozen as well as high-pressure frozen samples can be processed at cryo temperature by standard FIB milling and cryo-lift out approaches, respectively. Automated FIB milling routines based on commercial and in-house developed software packages enable unsupervised overnight operations for high-throughput milling.
Unique features
- Automated cryo lamella preparation
- Nanomanipulator for cryo-lift out
- Integrated widefield fluorescence microscopy
Important specifications
- Serial-FIB and AutoTEM software for automated lamella preparation
- EasyLift NanoManipulator
- Integrated Fluorescence Light Microscope (iFLM)
