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Protein quality control for downstream processes – Course and Conference Office

EMBL Course

Protein quality control for downstream processes

Overview

Registration is not yet open for this event. If you are interested in receiving more information please register your interest.

Course overview

Purified proteins are used in various types of scientific experiments and fields. For example, in structural biology, insights into the functional mechanisms can be obtained by elucidating the 3D molecular structure of proteins and protein complexes using technologies such as X-ray crystallography, cryo-EM and NMR.

In order for these experiments to produce reliable and biologically relevant results, they must be performed using high-quality proteins that are active, properly folded, in the right oligomeric state and contain correctly inserted co-factors. Ensuring that these parameters are fulfilled requires quality control.

In this course, we want to help the participants to become acquainted with the concept of protein quality control and provide them with an overview of the specific protein requirements for various downstream applications. Different techniques that can be applied for this purpose will be introduced and participants will have the opportunity to get hands-on experience with these technologies.

Audience

This course is aimed at life science researchers of all levels, who are using or plan to use purified proteins in their wet lab experiments.

After this course, participants should be familiar with the biochemical and biophysical techniques that can be applied to ensure that they are working with high-quality protein samples suitable for the planned downstream experiments.

Modules/resources

Basic Information

  • Protein expression and purification process
  • Protein concentration
  • Buffer conditions (pH, ionic strength, buffer components)

Purity and intactness

  • SDS-PAGE → right size, no degradation?
  • UV-VIS spectrum → nucleic acid contaminations?
  • Mass spectrometry → expected molecular weight and amino acid sequence?

Homogeneity/polydispersity

  • SEC, SEC-MALS → oligomeric state, no aggregates, molar mass?
  • DLS → presence of aggregates?
  • Mass photometry → oligomeric state, molar mass?
  • Flow Induced Dispersion Analysis → presence of aggregates?

Folding state and conformational stability:

  • Circular dichroism → secondary structure? influence of buffer conditions and/or ligands?
  • Nano-DSF, thermofluor → optimal buffer conditions? thermal stability?

Activity

  • Enzymatic assays → functionality?
  • Interaction studies: ITC, MST, Flow Induced Dispersion Analysis → affinity for binding partners?

Learning outcomes

During the course, specialists from both academia and industry will work closely together to introduce technologies frequently used in protein quality control. Some of these methodologies can be utilised both to assess protein quality and as standalone biophysical characterisation techniques.

Our main goals are:

  • Teach the participants how to perform proper protein quality control checks
  • Introduce the participants to various biophysical techniques used for protein characterization, such as SEC-MALS, DLS, SLS, mass photometry, DSF, flow induced dispersion analysis and spectroscopy
  • Show participants how to characterize protein interactions using ITC and flow induced dispersion analysis
  • When possible, allow participants to apply these technologies to characterise their own protein samples in the practicals
  • Overall, increase the success rate, reliability and reproducibility of downstream experiments in which purified proteins are used

Pre-requisites / technical requirements for joining the course

Participants are highly encouraged to bring their own sample(s) for protein quality control and biophysical characterization experiments during the practical sessions. This will provide an opportunity to directly apply the course content to their own research. However, bringing samples is not mandatory for course registration. Participants without samples are equally welcome and will still gain the full benefit of the training provided.

What past participants say about the course

“This advanced course was my first “live” experience at the EMBL and it was superb. It was packed with great lectures, state-of-the-art technology, fruitful discussions, and beautiful people. The course was also a great opportunity to meet new people and expand your network. Highly recommend and I will definitely attend the next event.” – Klemen Mezgec, National Institute of Chemistry Ljubljana, Slovenia

Speakers

Speakers, trainers and staff

Amit Meir Ben Efraim

Amit Meir Ben Efraim

University of Glasgow

UK

Markus Epe

Appled PhotoPhysics

Germany

Felix Gloge

Waters/Wyatt

Germany

To be confirmed

Waters/Wyatt

Germany

David Haak

FidaBio

Germany

Hasse Hedeby

FidaBio

Danmark

Sebastian Joecks

Refeyn

Germany

Karine Lapouge

EMBL Heidelberg

Germany

Kelvin Lau

EPFL

Switzerland

André Matagne

Université de Liège

Belgium

Bertrand Raynal

Institut Pasteur

France

Kim Remans

EMBL Heidelberg

Germany

Mandy Rettel

EMBL Heidelberg

Germany

Agatha Rosenthal

Malvern Pananalytical

UK

Kritika Sahni Ray

FidaBio

Denmark

Jan Schaeferkordt

Refeyn

Germany

Sascha Schifrin

Waters/Wyatt

Germany

Peter Spies

FidaBio

Denmark

Bernd Tartsch

Malvern PanAnlytical

Germany

Anne Zemella

Fraunhofer IZI-BB

Germany

Scientific organisers

Karine Lapouge

EMBL Heidelberg

Germany

Kim Remans

EMBL Heidelberg

Germany

Bertrand Raynal

Institut Pasteur

France

Course organisers

Lucía Rodriguez Minutta

Course and Conference Officer

EMBL Heidelberg

Germany

Michelle Steinhauer

Scientific Training Officer

EMBL Heidelberg

Germany

Programme

Are you on social media? Post using #EMBLProteinQC and don’t forget to tag @EMBLEvents

Day 1 – Monday 09 March 2026
Time (Europe/Berlin)SpeakerLocation
14:00 – 14:15Bus from hotel to EMBL ATC EntranceISG Hotel
14:30 – 15:00Welcome, house notes, course overview
Karine Lapouge – EMBL Heidelberg, Germany
Courtyard Room A & B
15:00 – 15:45Lecture 1, part 1: An overview of protein expression and purification methods for obtaining high-quality protein samples
Kim Remans – EMBL Heidelberg, Germany
Courtyard Room A & B
15:45 – 16:15Coffee Break Courtyard Room A & B
16:15 – 17:05Flash Talks round 1 (10 students)Courtyard Room A & B
17:05 – 17:55Lecture 2: Assessing and improving protein sample quality
Betrand Raynal – Institut Pasteur, France
Courtyard Room A & B
17:55 – 18:10Coffee BreakCourtyard Room A & B
18:10 – 19:00Flash Talks round 2 (10 students)Courtyard Room A & B
19:00 – 20:30DinnerATC
Rooftop bar
Day 2 – Tuesday 10 March 2026
Time (Europe/Berlin)SpeakerLocation
08:30 – 08:45Bus from hotel to EMBL ATC EntranceISG Hotel
09:00 – 09:10Overview of the dayCourtyard Room A & B
09:10 – 10:00
Lecture 1, part 2: An overview of protein expression and purification methods for obtaining high-quality protein samples
Kim Remans – EMBL Heidelberg, Germany
Courtyard Room A & B
10:00 – 10:30Applied PhotoPhysics introductionCourtyard Room A & B
10:30 – 11:00FidaBio introductionCourtyard Room A & B
11:00 – 11:15
Coffee breakCourtyard Room A & B
11:15 – 11:45Malvern introductionCourtyard Room A & B
11:45 – 12:15Refeyn introductionCourtyard Room A & B
12:15 – 12:45Wyatt/Waters introductionCourtyard Room A & B
12:45 – 13:45LunchEMBL Canteen
13:45 – 14:45Lecture 3: Membrane proteins
Amit Meir Ben Efraim – University of Glasgow, UK
Courtyard Room A & B
14:45 – 15:00Coffee breakCourtyard Room A & B
15:00 – 18:30Practical session 1Training Lab A & B
18:30 – 20:00DinnerTo be confirmed
20:00 – 20:30Bus from EMBL to HotelEMBL bus stop
Day 3 – Wednesday 11 March 2026
Time (Europe/Berlin)SpeakerLocation
08:30 – 08:45
Bus from hotel to EMBL ATC EntranceISG Hotel
09:00 – 09:10Overview of the dayCourtyard
Room A & B
12:40 – 13:30LunchEMBL
Canteen
13:30 – 14:45Lecture 4: Spectroscopy Methods for Protein Quality Control
Andre Matagne – University of Liege, Belgium
Courtyard
Room A & B
14:45 – 15:15Coffee breakCourtyard
Room A & B
15:15 – 18:45Practical session 3Training Lab
A & B
15:00 – 15:30Coffee breakCourtyard
Room A & B
18:45 – 20:00DinnerTo be confirmed
20:00 – 20:15Bus from EMBL to HotelEMBL bus stop
Day 4 – Thursday 12 March 2026
Time (Europe/Berlin)SpeakerLocation
08:30 – 08:45Bus from hotel to EMBL ATC EntranceISG Hotel
09:00 – 09:10Overview of the dayCourtyard
Room A & B
9:10 – 12:40Practical session 4Training Lab
A & B
12:40 – 13:30LunchEMBL
Canteen
13:30 – 14:20Lecture 5: Mass spectroscopy methods used in protein characterisation and quality control
Mandy Rettel – EMBL Heidelberg, Germany
Courtyard Room A & B
14:20 – 15:10Lecture 6: To be confirmed
Kelvin Lau – EPFL Lausanne, Switzerland
Courtyard Room A & B
15:10 – 15:40Coffee BreakCourtyard Room A & B
15:40 – 16:30Lecture 7: Cell-free
Anne-Zemella – Fraunhofer IZI-BB, Germany
Courtyard Room A & B
16:30 – 17:00PEPCF tour groups 1 and 2
Data collection groups 3 and 4
PEPCF EMBL
Training Lab
A & B
17:00 – 17:30PEPCF tour groups 3 and 4
Data collection groups 1 and 2
PEPCF EMBL
Training Lab
A & B
17:30 – 18:00Bus from EMBL to Heidelberg downtownEMBL bus stop
18:00 – 19:00Free time at Heidelberg downtown
19:00 – 21:00Dinner downtownTo be
confirmed
21:00 – 21:30Available bus to ISG Hotel
Day 5 – Friday 13 March 2026
Time (Europe/Berlin)SpeakerLocation
08:30 – 08:45Bus from hotel to EMBL ATC EntranceISG Hotel
09:00 – 09:10Overview of the dayCourtyard
Room A & B
9:10 – 12:00Preparation of student presentationsCourtyard
Room A & B
12:00 – 13:00LunchEMBL
Canteen
13:00 – 14:00Discussion group 1Courtyard
Room A & B
14:00 – 15:00Discussion group 2Courtyard
Room A & B
15:00 – 15:30Coffee breakCourtyard
Room A & B
15:30 – 16:30Discussion group 3Courtyard
Room A & B
16:30 – 17:30Discussion group 4Courtyard
Room A & B
17:30 – 18:00Feed-back and closing remarksCourtyard
Room A & B

Practical information

Registration fees and motivation letter

The course is limited to 20 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.

Registration

Registration fees include admission, course materials, meals and coffee breaks. Participants are expected to book and pay their own accommodation and travel expenses. 

Academia€ 740
PhD Student€ 740
Industry€ 1250

A letter to support your visa application will be issued, on request, once payment of the registration fee is confirmed. We recommend that you book your visa appointment as soon as possible, to avoid any delay with your visa application.

Confirmation and payment

The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.

Motivation letter submission

After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.

Instructions

  1. Include your relevant skills, experience and qualifications showing why you would be suitable for the course.
  2. Explain why you would like to attend, including what you can contribute and how you think you will benefit from the course.

Please note:

  • The limit of 2000 characters refers to manually typed text and excludes spaces. If an error occurs try using a different web browser (preferably Google Chrome or Mozilla Firefox).
  • If you copy-paste the text into the form, hidden formatting might still be included which may cause the text to exceed the 2,000 character limit resulting in an error message. We recommend you clear all formatting before pasting in the text.
  • If you have special symbols in your text, make sure you are using Unicode characters, otherwise these will not be recognised.

For detailed instructions, please watch our video on how to submit a course motivation letter.

Further details

For further information about registration and motivation letter submission please refer to the FAQ page.

Financial assistance

Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of registration fee waiverstravel grants and childcare grants

Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.

Registration fee waiver

The fee waiver will cover the registration sum that you have paid to attend the course.

Travel grant

The travel grant will cover the cost of travel (airfare, train, bus, taxi, accommodation, visa, and/or registration fees*) and is provided up to specified caps which are normally as follows:

– up to €400 for participants travelling to an EMBL Course within Europe.

– up to €1000 for participants travelling to an EMBL Course from outside Europe.

The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.

Childcare grant

There is the possibility to apply for a childcare grant to offset child care costs incurred by participants, speakers, trainers and organisers when attending a course. Eligible costs include (but are not limited to) fees for a babysitter or child-care facility and travel costs for a caregiver. Please note that priority will be given to early-stage researchers. There is a limited amount of funding available for the childcare grants and funds will be distributed amongst eligible applicants.

Boehringer Ingelheim Fonds travel grant

Applies to selected courses only. Availability will be indicated during the motivation letter submission process.

This grant covers costs related to your attendance at the course (registration, travel and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.

Whether you are eligible to apply for a travel grant, depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):

– for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course

– for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course

Application

You may apply for financial assistance when submitting your motivation letter for courses. In your application, you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.

For the Boehringer Ingelheim Fonds Travel Grant, there is a pre-application question during the motivation letter submission process, and if selected you will be requested to complete a standard form and documentation consisting of your travel expense estimation.

Selection

The scientific organisers will select the recipients of all financial assistance during the motivation letter selection process. Results will be announced approximately 6-8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the course. Selection is based on scientific merit, your current work or study location, the reasons for needing financial support, and the impact this event will have on your career.

Reimbursement

Costs will be reimbursed after the meeting only once a reimbursement form and original receipts (from travel costs) have been received.

Further details

See our list of external funding opportunities and for further information about financial assistance please refer to the FAQ page.

Accommodation and travel

Travel information

For travel information, please see here.

If you are travelling to the conference within Germany then you are eligible for the Deutsche Bahn ‘Event Ticket’ (called the ‘Veranstaltungsticket’ in German). This will result in a lower ticket price if your travel distance to Heidelberg is more than 100 km. You need to provide proof of your event attendance when purchasing the ticket.

For more information in English see here or in German see here.

You can book your ticket here.

Hotel

Accommodation is not included in the registration fee. Participants are expected to book and pay for their own accommodation. A mix of single and twin rooms have been reserved for 4 nights (09 – 13 March 2026) at the Hotel ISG Heidelberg.  Single rooms are limited and will be available on a first come, first served basis. After the selection process, selected participants should contact the hotel directly to make a booking with a booking code (code of your course e.g. PQC26-01) by the deadline of 6 February 2026. We recommend that all course participants stay in the dedicated hotel for the course.

Local transport

Shuttle buses will go from the Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.

Further details

Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

For more information about accommodation and travel, please refer to the FAQ page

On-site information

Travel information

For travel information, please see here.

If you are travelling to the conference within Germany then you are eligible for the Deutsche Bahn ‘Event Ticket’ (called the ‘Veranstaltungsticket’ in German). This will result in a lower ticket price if your travel distance to Heidelberg is more than 100 km. You need to provide proof of your event attendance when purchasing the ticket.

For more information in English see here or in German see here.

You can book your ticket here.

Catering

All meals and coffee breaks are included in the registration fee. Our catering staff will prepare a wide variety of vegetarian meals, meat and fish dishes, soups, pasta, fresh fruit and vegetables, as well as a variety of desserts.

Please wear your badge at all times when serving yourself.

No food or drinks are allowed in the laboratories.

Electricity and charging station

In most places the electricity is 220 volts AC (50 cycles). An adaptor and a plug that fits the German socket may be needed for your appliances/laptop (i.e. American, Japanese, etc.). A USB charging station for electronic devices is available at the registration desk.

EMBL Merchandise

If you are interested in purchasing EMBL merchandise (products presented in the glass display in the registration area), please email the EMBL shop to place an order or get in contact with your Course Organiser.

Kindly note the EMBL shop is only open upon request and all purchases must be made in cash (Euros only).

Health and safety notes

Please read EMBL’s COVID-19 safety policy for on-site events.
Do not smoke in any EMBL building.
Eating and drinking is prohibited in all laboratories.
Do not enter any restricted areas or the laboratories unless instructed to do so.

If first aid is required …

  • The first aid room is located next to the ATC Registration Desk (room 10- 205).
  • Dial the Emergency number 222 from any EMBL internal phone only.
  • Where is the accident? What happened? How many injured?
  • Emergency number 06221-387 7821 from mobile phones.
  • Please report all accidents to the conference officer at the registration desk.

In case of fire …

  • Press the nearest fire alarm button or the Emergency number 222 from any internal phone.
  • A loud fire alarm will go off if an evacuation is required. On hearing the alarm leave the building immediately following the escape route and fire wardens and head to one of the meeting points
  • Do not use the lifts.

Beyond first aid…

Please remember to bring your own medication, if needed, to the conference. Note that the next pharmacy is a 4-minute drive from the EMBL, but for many medications you will be required to see a doctor to get a prescription.

Ensure in advance that your medical insurance will cover you during your visit in the event that you do need to see a doctor while in Heidelberg. In any case, the EMBL Course and Conference Office will assist you to get to the pharmacy and a doctor of your choice if necessary.

Internet

Wi-Fi is available on campus. Log in using the EMBL-Events network and the event specific password, which will be provided on site. The eduroam network (secure, worldwide roaming access service developed for the international research and education community) is also available.

Lost and found

‘’Lost and Found’’ items are kept at the registration desk until the end of the conference.

Luggage

There are lockers available on-site to store your luggage, which require a 2 EURO coin to operate. There is another luggage room on level E0, which is free to use but remains unlocked during the conference.

Nursing room

There is a nursing room available in the ATC Rooftop Lounge on level A29.

Photography

During the conference, an EMBL Photographer may be taking photos. If you would not like to appear in these, please inform the photographer or a member of the Course and Conference Office.

Printing

We can help to print your boarding pass/train ticket. Please send it to events@embl.de and collect your print-outs at the registration desk.

Room for prayer, yoga, and meditation

There is a room for prayer, meditation, and yoga located on level E0 behind the Auditorium. Please be respectful of others using the room.

Sightseeing

A variety of activities in Heidelberg can be found on the website of Heidelberg Marketing.

Travel to and from the venue

During the event, we provide conference shuttle buses to and from EMBL. In addition, there is the public bus 39A that serves the EMBL campus and taxis can be easily booked at any time. Information on the shuttle buses can be found on the individual event website and more detailed information on travelling to EMBL can be found on our Travel Information page.

Additional information

Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.

Technical requirements and event software

Event software

The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus. 

Sponsors

Sponsorship opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other conferences, or contact sponsorship@embl.de for further information.

If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.

Warning

EMBL wishes to warn sponsors of EMBL conferences and courses of fraudulent schemes purporting to offer sponsorship opportunities on behalf of EMBL or affiliated with EMBL officials. One current scam campaign of which we are aware is conducted using the name ‘Judy Eastman’ (judy@gopcontact.a2hosted.com) and entails approaches to sponsors offering sponsorship opportunities on EMBL’s behalf. Please be kindly advised that all relevant communication regarding sponsorship of EMBL conferences, symposia and courses is handled by EMBL directly and is sent from an official EMBL account. EMBL does not work with any external providers on sponsorship acquisition.

Please also note that:

  • EMBL never provides attendee lists for purchase. Any offers of such are fraudulent.
  • EMBL will never call or email you to ask for your credit card details or to request a payment.
  • All payments are on invoice.

Suspicious communications purportedly from, for or on behalf of EMBL should be reported to EMBL at the following email address sponsoring@embl.de.

Media kit

Want to let others know you’re attending this event? Take a look at our shareable media and feel free to use them in your social media channels or presentations.

Date: 9 - 13 Mar 2026

Location: EMBL Heidelberg

Venue: EMBL Advanced Training Centre


Deadline(s):

Application: 17 Nov 2025


EMBL staff registration


Organisers:


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