Time-resolved STED nanoscopy in life sciences

Course overview

This six-day comprehensive microscopy course focuses on stimulated emission depletion microscopy (STED) and its application to biological questions. Through lectures, tutorials, demonstrations and practical workshops, the course provides in-depth knowledge on all aspects of STED Nanoscopy. It covers the fundamentals of STED microscopy, different technical implementations and has a strong emphasis on sample preparation – including choice of fluorophores and refractive index matching, as well as image acquisition strategies for fixed samples and living cells. Students will have the opportunity for exercises on state-of the art STED microscopes in which they will acquire high quality nanoscopy data. The faculty consists of Invited guest researchers, experts from the EMBL and Leica Microsystems.

Audience

This course is designed for researchers at the early stages of their career in the life sciences (e.g. pre or postdoctoral level), ideally with access to a STED microscope. The participants should have an immediate need to apply the trained skills for their biological questions. The course is strongly centered on practical aspects of STED microscopy and is therefore especially suited for people who rely on STED nanoscopy for their research.

Modules/Resources

The course will cover the entire range of skills needed to apply STED nanoscopy in biology. The following topics will be covered in lectures, tutorials as well as in practical workshops:

Sample preparation for STED microscopy, including choice of fluorophores and refractive index matching

Technical implementations – CW-STED, pulsed-STED, gated-STED, time-resolved STED (tauSTED)

Multi-Color 2D and 3D STED microscopy

Image acquisition strategies for live-cell STED

What past participants say about the course

“The EMBL super-resolution microscopy course was exactly tailored to my needs and will allow me to get my own experiments running independently. Also, contacts I made will be very useful in the future!” – Paul Markus Müller, Freie Universität Berlin, Germany

“Very well organized and conceived course. Everything is covered here including the
fundamentals, the pratical sessions, creative application, innovative development of STED technology. If you are interested in the nanoscopy, I would recommend you to participate in this course.” – Shao-Chun ‘Peggy’ Hsu, National Taiwan University, Taiwan

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