Time-resolved STED nanoscopy in life sciences – Course and Conference Office

EMBL Course

Time-resolved STED nanoscopy in life sciences


Registration is not yet open for this event. If you are interested in receiving more information please register your interest.

Course overview

This six-day comprehensive microscopy course focuses on stimulated emission depletion microscopy (STED) and its application to biological questions. Through lectures, tutorials, demonstrations and practical workshops, the course provides in-depth knowledge on all aspects of STED Nanoscopy. It covers the fundamentals of STED microscopy, different technical implementations and has a strong emphasis on sample preparation – including choice of fluorophores and refractive index matching, as well as image acquisition strategies for fixed samples and living cells. Students will have the opportunity for exercises on state-of the art STED microscopes in which they will acquire high quality nanoscopy data. The faculty consists of Invited guest researchers, experts from the EMBL and Leica Microsystems.


This course is designed for researchers at the early stages of their career in the life sciences (e.g. pre or postdoctoral level), ideally with access to a STED microscope. The participants should have an immediate need to apply the trained skills for their biological questions. The course is strongly centered on practical aspects of STED microscopy and is therefore especially suited for people who rely on STED nanoscopy for their research.


The course will cover the entire range of skills needed to apply STED nanoscopy in biology. The following topics will be covered in lectures, tutorials as well as in practical workshops:

  • Sample preparation for STED microscopy, including choice of fluorophores and refractive index matching
  • Technical implementations – CW-STED, pulsed-STED, gated-STED, time-resolved STED (tauSTED)
  • Multi-Color 2D and 3D STED microscopy
  • Image acquisition strategies for live-cell STED

Learning Outcomes

At the end of the course, the participants will be able to design experiments using STED microscopy, prepare high-quality microscopy samples and acquire state-of-the-art nanoscopy images.

This course is organised in cooperation with Leica Microsystems.

What past participants say about the course

“The EMBL super-resolution microscopy course was exactly tailored to my needs and will allow me to get my own experiments running independently. Also, contacts I made will be very useful in the future!” – Paul Markus Müller, Freie Universität Berlin, Germany

“Very well organized and conceived course. Everything is covered here including the
fundamentals, the pratical sessions, creative application, innovative development of STED technology. If you are interested in the nanoscopy, I would recommend you to participate in this course.”
– Shao-Chun ‘Peggy’ Hsu, National Taiwan University, Taiwan


Sponsorship opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other events, or contact sponsorship@embl.de for further information.

If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.


EMBL wishes to warn sponsors of EMBL conferences and courses of fraudulent schemes purporting to offer sponsorship opportunities on behalf of EMBL or affiliated with EMBL officials. One current scam campaign of which we are aware is conducted using the name ‘Judy Eastman’ (judy@gopcontact.a2hosted.com) and entails approaches to sponsors offering sponsorship opportunities on EMBL’s behalf. Please be kindly advised that all relevant communication regarding sponsorship of EMBL conferences, symposia and courses is handled by EMBL directly and is sent from an official EMBL account. EMBL does not work with any external providers on sponsorship acquisition.

Please also note that:

  • EMBL never provides attendee lists for purchase. Any offers of such are fraudulent.
  • EMBL will never call or email you to ask for your credit card details or to request a payment.
  • All payments are on invoice.

Suspicious communications purportedly from, for or on behalf of EMBL should be reported to EMBL at the following email address sponsorship@embl.de.

Date: 8 - 13 Jul 2024

Location: EMBL Heidelberg

Venue: EMBL Advanced Training Centre

Contact: Luana Ribeiro

EMBL Courses and Conferences are kindly supported by our Corporate Partnership Programme

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